CK935118

Overview
NameCK935118
Unique NameCK935118
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length873
Libraries
Library NameType
Developing fruit peel at 38 DAFBcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CK935118_ssr9 microsatellite CK935118_ssr9:9..20. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: YPTM2_MAIZE (GTP-binding protein YPTM2 OS=Zea mays GN=YPTM2 PE=2 SV=1)

HSP 1 Score: 333.569 bits (854), Expect = 2.539e-95
Identity = 165/186 (88.71%), Postives = 175/186 (94.09%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCCST 761
            GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKT+KLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYAS+NVNKLLVGNK DLT  KVV+ ETAKAFADE+GIPFMETSAK++ NV+QAFMAM A IK+RMASQPA  NARP TVQI+GQPVNQK SCCS+
Sbjct:   18 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASDNVNKLLVGNKSDLTANKVVATETAKAFADEMGIPFMETSAKNATNVQQAFMAMAASIKDRMASQPAAANARPATVQIRGQPVNQKTSCCSS 203

HSP 2 Score: 37.7354 bits (86), Expect = 2.539e-95
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    1 MNPEYDYLFKLLLIGD 16
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RBD2A_ARATH (Ras-related protein RABD2A OS=Arabidopsis thaliana GN=RABD2A PE=1 SV=3)

HSP 1 Score: 333.569 bits (854), Expect = 2.539e-95
Identity = 162/186 (87.10%), Postives = 175/186 (94.09%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCCST 761
            GVGKSCLLLRF+DDSY++SYISTIGVDFKIRTVEQDGKT+KLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTD+ESFNNVKQWL+EIDRYAS+NVNKLLVGNK DLT+ + + YETAKAFADEIGIPFMETSAKD+ NVEQAFMAM+A IK RMASQPA NNARPPTVQI+GQPV QK  CCST
Sbjct:   18 GVGKSCLLLRFSDDSYVESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDEESFNNVKQWLSEIDRYASDNVNKLLVGNKSDLTENRAIPYETAKAFADEIGIPFMETSAKDATNVEQAFMAMSASIKERMASQPAGNNARPPTVQIRGQPVAQKNGCCST 203

HSP 2 Score: 37.7354 bits (86), Expect = 2.539e-95
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    1 MNPEYDYLFKLLLIGD 16
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RIC1_ORYSJ (Ras-related protein RIC1 OS=Oryza sativa subsp. japonica GN=RIC1 PE=2 SV=2)

HSP 1 Score: 327.02 bits (837), Expect = 2.332e-93
Identity = 157/185 (84.86%), Postives = 172/185 (92.97%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCCS 758
            GVGKSCLLLRFADDSYL+SYISTIGVDFKIRTVEQDGKT+KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDL + +VVSYE  KA ADEIGIPF+ETSAKD+ NVE+AFM M  +IKNRMASQPA N ++P TVQ++GQPV Q++SCCS
Sbjct:   18 GVGKSCLLLRFADDSYLESYISTIGVDFKIRTVEQDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLAENRVVSYEAGKALADEIGIPFLETSAKDATNVEKAFMTMAGEIKNRMASQPATNASKPATVQMRGQPVAQQSSCCS 202

HSP 2 Score: 37.7354 bits (86), Expect = 2.332e-93
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    1 MNPEYDYLFKLLLIGD 16
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: YPTC1_CHLRE (GTP-binding protein YPTC1 OS=Chlamydomonas reinhardtii GN=YPTC1 PE=3 SV=1)

HSP 1 Score: 301.982 bits (772), Expect = 7.440e-86
Identity = 150/185 (81.08%), Postives = 163/185 (88.11%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQ-IKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRTVE DGK +KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNK DLT KKVV Y  AKAFADEIGIPF+ETSAK++ NVEQAFM M A+IKNRMASQP    A  P V+  +G+P+N K+S C
Sbjct:   18 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTVELDGKVIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKSDLTSKKVVEYSVAKAFADEIGIPFLETSAKNATNVEQAFMTMAAEIKNRMASQPIPTKAGGPVVRPQEGKPINSKSSSC 202

HSP 2 Score: 37.7354 bits (86), Expect = 7.440e-86
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    1 MNPEYDYLFKLLLIGD 16
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: YPTV1_VOLCA (GTP-binding protein yptV1 OS=Volvox carteri GN=YPTV1 PE=3 SV=1)

HSP 1 Score: 297.745 bits (761), Expect = 1.384e-84
Identity = 148/185 (80.00%), Postives = 162/185 (87.57%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQ-IKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRTVE DGK +KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWL EIDRYASENVNKLLVGNK DLT KKVV Y+ AKAFADEIGIPF+ETSAK++ NVEQAFM M A+IKNRMASQP       P V+  +G+P+N K+S C
Sbjct:   18 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTVELDGKVIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLAEIDRYASENVNKLLVGNKSDLTGKKVVDYQAAKAFADEIGIPFLETSAKNATNVEQAFMTMAAEIKNRMASQPVPPKPGGPVVRPTEGKPINNKSSSC 202

HSP 2 Score: 37.7354 bits (86), Expect = 1.384e-84
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    1 MNPEYDYLFKLLLIGD 16
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RAB1A_RAT (Ras-related protein Rab-1A OS=Rattus norvegicus GN=Rab1A PE=1 SV=3)

HSP 1 Score: 287.345 bits (734), Expect = 1.808e-81
Identity = 140/184 (76.09%), Postives = 153/184 (83.15%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRT+E DGKT+KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWL EIDRYASENVNKLLVGNKCDLT KKVV Y TAK FAD +GIPF+ETSAK++ NVEQ+FM M A+IK RM        A    V+I+  PV Q    C
Sbjct:   21 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGC 204

HSP 2 Score: 37.7354 bits (86), Expect = 1.808e-81
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    4 MNPEYDYLFKLLLIGD 19
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RAB1A_MOUSE (Ras-related protein Rab-1A OS=Mus musculus GN=Rab1A PE=1 SV=3)

HSP 1 Score: 287.345 bits (734), Expect = 1.808e-81
Identity = 140/184 (76.09%), Postives = 153/184 (83.15%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRT+E DGKT+KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWL EIDRYASENVNKLLVGNKCDLT KKVV Y TAK FAD +GIPF+ETSAK++ NVEQ+FM M A+IK RM        A    V+I+  PV Q    C
Sbjct:   21 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGC 204

HSP 2 Score: 37.7354 bits (86), Expect = 1.808e-81
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    4 MNPEYDYLFKLLLIGD 19
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RAB1A_HUMAN (Ras-related protein Rab-1A OS=Homo sapiens GN=RAB1A PE=1 SV=3)

HSP 1 Score: 287.345 bits (734), Expect = 1.808e-81
Identity = 140/184 (76.09%), Postives = 153/184 (83.15%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRT+E DGKT+KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWL EIDRYASENVNKLLVGNKCDLT KKVV Y TAK FAD +GIPF+ETSAK++ NVEQ+FM M A+IK RM        A    V+I+  PV Q    C
Sbjct:   21 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGC 204

HSP 2 Score: 37.7354 bits (86), Expect = 1.808e-81
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    4 MNPEYDYLFKLLLIGD 19
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RAB1A_CANFA (Ras-related protein Rab-1A OS=Canis familiaris GN=RAB1A PE=1 SV=3)

HSP 1 Score: 287.345 bits (734), Expect = 1.808e-81
Identity = 140/184 (76.09%), Postives = 153/184 (83.15%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRT+E DGKT+KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWL EIDRYASENVNKLLVGNKCDLT KKVV Y TAK FAD +GIPF+ETSAK++ NVEQ+FM M A+IK RM        A    V+I+  PV Q    C
Sbjct:   21 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQSFMTMAAEIKKRMGPGATAGGAEKSNVKIQSTPVKQSGGGC 204

HSP 2 Score: 37.7354 bits (86), Expect = 1.808e-81
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    4 MNPEYDYLFKLLLIGD 19
[Close]
BLAST of CK935118 vs. ExPASy Swiss-Prot
Match: RAB1_DISOM (Ras-related protein ORAB-1 OS=Discopyge ommata PE=2 SV=1)

HSP 1 Score: 285.034 bits (728), Expect = 8.907e-81
Identity = 139/184 (75.54%), Postives = 151/184 (82.07%), Query Frame = 3 
Query:  204 GVGKSCLLLRFADDSYLDSYISTIGVDFKIRTVEQDGKTVKLQIWDTAGQERFRTITSSYYRGAHGIIIVYDVTDQESFNNVKQWLNEIDRYASENVNKLLVGNKCDLTDKKVVSYETAKAFADEIGIPFMETSAKDSMNVEQAFMAMTADIKNRMASQPAMNNARPPTVQIKGQPVNQKASCC 755
            GVGKSCLLLRFADD+Y +SYISTIGVDFKIRT+E DGKT+KLQIWDTAGQERFRTITSSYYRGAHGII+VYDVTDQESFNNVKQWL EIDRYASENVNKLLVGNKCDLT KKVV Y TAK FAD +GIPF+ETSAK++ NVEQAFM M A+IK RM        +    V I+  PV      C
Sbjct:   18 GVGKSCLLLRFADDTYTESYISTIGVDFKIRTIELDGKTIKLQIWDTAGQERFRTITSSYYRGAHGIIVVYDVTDQESFNNVKQWLQEIDRYASENVNKLLVGNKCDLTTKKVVDYTTAKEFADSLGIPFLETSAKNATNVEQAFMTMAAEIKKRMGPGATSGGSEKSNVNIQSTPVKSSGGGC 201

HSP 2 Score: 37.7354 bits (86), Expect = 8.907e-81
Identity = 16/16 (100.00%), Postives = 16/16 (100.00%), Query Frame = 1 
Query:  154 MNPEYDYLFKLLLIGD 201
            MNPEYDYLFKLLLIGD
Sbjct:    1 MNPEYDYLFKLLLIGD 16
The following BLAST results are available for this feature:
BLAST of CK935118 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
YPTM2_MAIZE2.539e-9588.71GTP-binding protein YPTM2 OS=Zea mays GN=YPTM2 PE=... [more]
RBD2A_ARATH2.539e-9587.10Ras-related protein RABD2A OS=Arabidopsis thaliana... [more]
RIC1_ORYSJ2.332e-9384.86Ras-related protein RIC1 OS=Oryza sativa subsp. ja... [more]
YPTC1_CHLRE7.440e-8681.08GTP-binding protein YPTC1 OS=Chlamydomonas reinhar... [more]
YPTV1_VOLCA1.384e-8480.00GTP-binding protein yptV1 OS=Volvox carteri GN=YPT... [more]
RAB1A_RAT1.808e-8176.09Ras-related protein Rab-1A OS=Rattus norvegicus GN... [more]
RAB1A_MOUSE1.808e-8176.09Ras-related protein Rab-1A OS=Mus musculus GN=Rab1... [more]
RAB1A_HUMAN1.808e-8176.09Ras-related protein Rab-1A OS=Homo sapiens GN=RAB1... [more]
RAB1A_CANFA1.808e-8176.09Ras-related protein Rab-1A OS=Canis familiaris GN=... [more]
RAB1_DISOM8.907e-8175.54Ras-related protein ORAB-1 OS=Discopyge ommata PE=... [more]

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Properties
Property NameValue
Genbank descriptionCGF1004177_G05 Developing fruit peel at 38 DAFB Citrus sinensis cDNA clone P38DA0001_IIF_G05 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CK935118 ID=CK935118; Name=CK935118; organism=Citrus sinensis; type=EST; length=873bp
GGGGGGTTTCATCATCATCATCGACATTTTGCTTTCTTCGTCAAATTCTC
TCTCAATCTCACCTCCTTAGCTTGTCTGTGCCAAACTCCCGGTCCAATCT
CCAGCTGGACCGGTTCGATTCTGCGCGAACCCCCTCAACATCTTATTGCA
GTCATGAATCCTGAATATGACTACTTGTTCAAGCTTTTGCTGATTGGAGA
CTTGGAGTTGGCAAATCGTGTCTTCTTCTGAGATTTGCTGATGATTCGTA
CCTGGACAGTTACATTAGCACTATTGGGGTTGACTTTAAAATTCGCACAG
TGGAGCAAGACGGGAAGACTGTAAAACTCCAAATTTGGGACACTGCAGGG
CAAGAACGATTCAGGACAATCACTAGCAGCTACTACCGTGGAGCACATGG
CATTATCATTGTTTATGATGTTACAGACCAAGAGAGCTTCAACAACGTTA
AGCAGTGGTTGAATGAAATTGATCGCTATGCAAGTGAAAATGTAAACAAG
CTTCTGGTTGGAAACAAGTGTGATCTCACTGATAAGAAGGTTGTCTCTTA
TGAAACAGCGAAGGCATTTGCTGATGAAATTGGCATCCCGTTCATGGAGA
CCAGCGCAAAGGATTCTATGAACGTGGAACAAGCTTTTATGGCTATGACT
GCTGACATTAAGAACAGGATGGCAAGTCAACCTGCAATGAACAATGCAAG
GCCACCAACTGTGCAGATAAAAGGACAACCGGTGAACCAGAAGGCCAGCT
GCTGCTCAACTTAATCGTTTGTCTTATCCTTCATTGCGTGCATTTGTTTA
TGGGTAGCTTTCTCAATCACGTTGTAGGAACCAGTGAAAAACTATTCCTA
CCCCATGAATCATATGACGGATT
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