EY736074 | Citrus Genome Database

Overview


Name	EY736074
Unique Name	EY736074
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	854

Libraries


Library Name	Type
Sweet orange fruit, development stadium (6 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_MAIZE (Soluble inorganic pyrophosphatase OS=Zea mays GN=IPP PE=2 SV=1)

HSP 1 Score: 270.781 bits (691), Expect = 5.616e-82
Identity = 129/159 (81.13%), Postives = 140/159 (88.05%), Query Frame = 3

Query:  231 ETSSKTPVSQPPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKEL-PH 704
            E  +     QP   P LNERILSS+SRRSVAAHPWHDLEIGP AP +FN V+EI KGSKVKYELDKK+GLI VDRVLYSSVVYPHNYGF+PRTLCEDNDP+DVL++MQEPV+PG FLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDI EL PH
Sbjct:    4 EDKTAASAEQPKRAPKLNERILSSLSRRSVAAHPWHDLEIGPDAPAVFNVVVEITKGSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGFVPRTLCEDNDPMDVLVLMQEPVVPGSFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDISELSPH 162

HSP 2 Score: 42.3578 bits (98), Expect = 5.616e-82
Identity = 19/35 (54.29%), Postives = 28/35 (80.00%), Query Frame = 1

Query:  742 QENENKEVAVNDFLPASDALXSVXQSMNLYADYMV 846
            ++NENKEVAV+ FLPA+ A  ++  SM+LYA Y++
Sbjct:  175 KKNENKEVAVDAFLPATTAREAIQYSMDLYAQYIL 209

HSP 3 Score: 34.2686 bits (77), Expect = 5.616e-82
Identity = 14/23 (60.87%), Postives = 18/23 (78.26%), Query Frame = 2

Query:  698 STHRLAEIRRFFEDYKKMRTRKL 766
            S HRL EI+RFFEDYKK   +++
Sbjct:  160 SPHRLQEIKRFFEDYKKNENKEV 182

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_ORYSJ (Soluble inorganic pyrophosphatase OS=Oryza sativa subsp. japonica GN=IPP PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 5.616e-82
Identity = 126/159 (79.25%), Postives = 140/159 (88.05%), Query Frame = 3

Query:  231 ETSSKTPVSQ--PPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            E   K P+    PPA   LNERILSSMS++ VAAHPWHDLEIGPGAP +FNCV+EI +GSKVKYELDK +GLI VDRVLYSSVVYPHNYGFIPRTLCED DP+DVL++MQE V+PGCFLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRH+ DIKE+P
Sbjct:    4 EADGKAPLGSRYPPA--ALNERILSSMSQKHVAAHPWHDLEIGPGAPAVFNCVVEIPRGSKVKYELDKATGLIKVDRVLYSSVVYPHNYGFIPRTLCEDGDPMDVLVLMQEQVVPGCFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHFRDIKEIP 160

HSP 2 Score: 48.1358 bits (113), Expect = 5.616e-82
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = 1

Query:  742 QENENKEVAVNDFLPASDALXSVXQSMNLYADYMV 846
            ++NENKEVAVN+FLPA DA+ ++  SM+LY  Y++
Sbjct:  175 KKNENKEVAVNEFLPAEDAINAIKYSMDLYGAYII 209

HSP 3 Score: 34.2686 bits (77), Expect = 5.616e-82
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 2

Query:  704 HRLAEIRRFFEDYKKMRTRKL 766
            HRL EIRRFFEDYKK   +++
Sbjct:  162 HRLQEIRRFFEDYKKNENKEV 182

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_ORYSI (Soluble inorganic pyrophosphatase OS=Oryza sativa subsp. indica GN=IPP PE=2 SV=1)

HSP 1 Score: 265.003 bits (676), Expect = 5.616e-82
Identity = 126/159 (79.25%), Postives = 140/159 (88.05%), Query Frame = 3

Query:  231 ETSSKTPVSQ--PPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            E   K P+    PPA   LNERILSSMS++ VAAHPWHDLEIGPGAP +FNCV+EI +GSKVKYELDK +GLI VDRVLYSSVVYPHNYGFIPRTLCED DP+DVL++MQE V+PGCFLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRH+ DIKE+P
Sbjct:    4 EADGKAPLGSRYPPA--ALNERILSSMSQKHVAAHPWHDLEIGPGAPAVFNCVVEIPRGSKVKYELDKATGLIKVDRVLYSSVVYPHNYGFIPRTLCEDGDPMDVLVLMQEQVVPGCFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHFRDIKEIP 160

HSP 2 Score: 48.1358 bits (113), Expect = 5.616e-82
Identity = 20/35 (57.14%), Postives = 29/35 (82.86%), Query Frame = 1

Query:  742 QENENKEVAVNDFLPASDALXSVXQSMNLYADYMV 846
            ++NENKEVAVN+FLPA DA+ ++  SM+LY  Y++
Sbjct:  175 KKNENKEVAVNEFLPAEDAINAIKYSMDLYGAYII 209

HSP 3 Score: 34.2686 bits (77), Expect = 5.616e-82
Identity = 14/21 (66.67%), Postives = 17/21 (80.95%), Query Frame = 2

Query:  704 HRLAEIRRFFEDYKKMRTRKL 766
            HRL EIRRFFEDYKK   +++
Sbjct:  162 HRLQEIRRFFEDYKKNENKEV 182

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_SOLTU (Soluble inorganic pyrophosphatase OS=Solanum tuberosum GN=PPA PE=2 SV=1)

HSP 1 Score: 268.855 bits (686), Expect = 3.085e-78
Identity = 126/143 (88.11%), Postives = 135/143 (94.41%), Query Frame = 3

Query:  273 PPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            P LNERILSS+SRRSVAAHPWHDLEIGP AP +FN VIEI KGSKVKYELDKK+GLI VDR+LYSSVVYP NYGFIPRTLCEDNDP+DVL++MQEPVLPGCFLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRHY DIK+LP
Sbjct:   15 PRLNERILSSISRRSVAAHPWHDLEIGPEAPSVFNVVIEISKGSKVKYELDKKTGLIKVDRILYSSVVYPQNYGFIPRTLCEDNDPMDVLVLMQEPVLPGCFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHYTDIKQLP 157

HSP 2 Score: 44.669 bits (104), Expect = 3.085e-78
Identity = 18/35 (51.43%), Postives = 30/35 (85.71%), Query Frame = 1

Query:  742 QENENKEVAVNDFLPASDALXSVXQSMNLYADYMV 846
            ++NENK+VAV+DFLP + A+ ++  SM+LYA+Y++
Sbjct:  172 KKNENKDVAVDDFLPPNSAVNAIQYSMDLYAEYIL 206

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR2_ARATH (Soluble inorganic pyrophosphatase 2 OS=Arabidopsis thaliana GN=PPA2 PE=2 SV=2)

HSP 1 Score: 249.21 bits (635), Expect = 2.569e-75
Identity = 114/141 (80.85%), Postives = 127/141 (90.07%), Query Frame = 3

Query:  279 LNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            LNER  ++ + RS AAHPWHDLEIGP AP +FNCV+EI KG KVKYELDK SGLI VDRVLYSS+VYPHNYGFIPRT+CED+DP+DVL++MQEPVL G FLRA+AIGLMPMIDQGEKDDKIIAVCADDPE+RHY DIKELP
Sbjct:   24 LNERNFAAFTHRSAAAHPWHDLEIGPEAPTVFNCVVEISKGGKVKYELDKNSGLIKVDRVLYSSIVYPHNYGFIPRTICEDSDPMDVLVLMQEPVLTGSFLRARAIGLMPMIDQGEKDDKIIAVCADDPEFRHYRDIKELP 164

HSP 2 Score: 38.1206 bits (87), Expect = 2.569e-75
Identity = 17/34 (50.00%), Postives = 25/34 (73.53%), Query Frame = 1

Query:  742 QENENKEVAVNDFLPASDALXSVXQSMNLYADYM 843
            ++NENK+V V  FLPA  A+ ++  SM+LYA Y+
Sbjct:  179 KKNENKKVDVEAFLPAQAAIDAIKDSMDLYAAYI 212

HSP 3 Score: 37.7354 bits (86), Expect = 2.569e-75
Identity = 16/21 (76.19%), Postives = 18/21 (85.71%), Query Frame = 2

Query:  704 HRLAEIRRFFEDYKKMRTRKL 766
            HRLAEIRRFFEDYKK   +K+
Sbjct:  166 HRLAEIRRFFEDYKKNENKKV 186

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_HORVD (Soluble inorganic pyrophosphatase OS=Hordeum vulgare var. distichum GN=IPP PE=2 SV=1)

HSP 1 Score: 256.914 bits (655), Expect = 4.388e-74
Identity = 123/158 (77.85%), Postives = 139/158 (87.97%), Query Frame = 3

Query:  237 SSKTPVSQPPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYN-DIKELPHI 707
            +++ P S+P   P LNERILSS+SRR   AHPWHDLEIGPGAP +FN V+EI KGSKVKYELDKK+GLI VDRVLYSSVVYPHNYGFIPRTLCEDNDP+DVL++MQEPV+PG FLRA+AIGLMPMIDQGEKDDKIIAVCADDPEYRHY+  +  LP +
Sbjct:   11 AAQQPTSRPA--PKLNERILSSLSRRGGGAHPWHDLEIGPGAPAVFNVVVEITKGSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGFIPRTLCEDNDPMDVLVLMQEPVIPGSFLRARAIGLMPMIDQGEKDDKIIAVCADDPEYRHYSTSVSLLPRL 166

HSP 2 Score: 42.743 bits (99), Expect = 4.388e-74
Identity = 25/49 (51.02%), Postives = 32/49 (65.31%), Query Frame = 1

Query:  727 LL*RLQE---------NENKEVAVNDFLPASDALXSVXQSMNLYADYMV 846
            LL RLQE         NENKEVAV+ FLPA+ A  ++  SM+LYA Y++
Sbjct:  162 LLPRLQEIKRLEDYKKNENKEVAVDAFLPATTAREAIQYSMDLYAQYIL 210

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR2_CHLRE (Soluble inorganic pyrophosphatase 2 OS=Chlamydomonas reinhardtii GN=ppa2 PE=1 SV=1)

HSP 1 Score: 204.142 bits (518), Expect = 2.089e-60
Identity = 92/130 (70.77%), Postives = 109/130 (83.85%), Query Frame = 3

Query:  312 RSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            R  A+HPWHDL  G  AP   +CVIEI +GSKVKYELDK +GL  VDR+LYSSVVYPHNYGF+P+TLCED DPLDVL++MQEPV+P CFLRAK IG+M M+DQGE+DDK+IAV ADDPEY+ + DI +LP
Sbjct:    5 RGTASHPWHDLHPGNDAPNFVSCVIEIPRGSKVKYELDKDTGLCFVDRILYSSVVYPHNYGFVPKTLCEDGDPLDVLVLMQEPVVPMCFLRAKPIGVMQMLDQGERDDKLIAVHADDPEYKGFTDISQLP 134

HSP 2 Score: 36.1946 bits (82), Expect = 2.089e-60
Identity = 16/35 (45.71%), Postives = 25/35 (71.43%), Query Frame = 1

Query:  742 QENENKEVAVNDFLPASDALXSVXQSMNLYADYMV 846
            ++NE+KEV V+DFL A +A   V  S+N+Y ++ V
Sbjct:  149 KKNEHKEVVVDDFLGAEEAKKVVKDSLNMYQEHYV 183

HSP 3 Score: 34.6538 bits (78), Expect = 2.089e-60
Identity = 14/22 (63.64%), Postives = 19/22 (86.36%), Query Frame = 2

Query:  704 HRLAEIRRFFEDYKKMRTRKLL 769
            HRLAEI+RFFEDYKK   ++++
Sbjct:  136 HRLAEIKRFFEDYKKNEHKEVV 157

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_LEPIN (Inorganic pyrophosphatase OS=Leptospira interrogans GN=ppa PE=3 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 5.882e-39
Identity = 71/125 (56.80%), Postives = 92/125 (73.60%), Query Frame = 3

Query:  327 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            HPWHD+  G   P+I N VIEI +GS+ KYE+DK+ G++ +DRVLYSS  YP NYGFIP++ C D DPLD+L++ Q  + P C ++AK IG+M M+D GE+DDKIIAV A+D    H NDI ELP
Sbjct:    3 HPWHDISPGDQNPEIVNGVIEIKRGSRAKYEVDKEYGILKLDRVLYSSFYYPANYGFIPQSYCGDQDPLDILVLSQVELEPLCLVKAKVIGVMRMLDSGEEDDKIIAVAANDMSVNHINDISELP 127

HSP 2 Score: 26.5646 bits (57), Expect = 5.882e-39
Identity = 9/22 (40.91%), Postives = 15/22 (68.18%), Query Frame = 2

Query:  704 HRLAEIRRFFEDYKKMRTRKLL 769
            H   E++ FFEDYKK+  + ++
Sbjct:  129 HFTLELKHFFEDYKKLENKTVV 150

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_LEPIC (Inorganic pyrophosphatase OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=ppa PE=3 SV=1)

HSP 1 Score: 155.606 bits (392), Expect = 5.882e-39
Identity = 71/125 (56.80%), Postives = 92/125 (73.60%), Query Frame = 3

Query:  327 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            HPWHD+  G   P+I N VIEI +GS+ KYE+DK+ G++ +DRVLYSS  YP NYGFIP++ C D DPLD+L++ Q  + P C ++AK IG+M M+D GE+DDKIIAV A+D    H NDI ELP
Sbjct:    3 HPWHDISPGDQNPEIVNGVIEIKRGSRAKYEVDKEYGILKLDRVLYSSFYYPANYGFIPQSYCGDQDPLDILVLSQVELEPLCLVKAKVIGVMRMLDSGEEDDKIIAVAANDMSVNHINDISELP 127

HSP 2 Score: 26.5646 bits (57), Expect = 5.882e-39
Identity = 9/22 (40.91%), Postives = 15/22 (68.18%), Query Frame = 2

Query:  704 HRLAEIRRFFEDYKKMRTRKLL 769
            H   E++ FFEDYKK+  + ++
Sbjct:  129 HFTLELKHFFEDYKKLENKTVV 150

[Close]

BLAST of EY736074 vs. ExPASy Swiss-Prot
Match: IPYR_PYRFU (Inorganic pyrophosphatase OS=Pyrococcus furiosus GN=ppa PE=1 SV=1)

HSP 1 Score: 145.591 bits (366), Expect = 3.008e-34
Identity = 66/125 (52.80%), Postives = 92/125 (73.60%), Query Frame = 3

Query:  327 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHNYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLRAKAIGLMPMIDQGEKDDKIIAVCADDPEYRHYNDIKELP 701
            +P+HDLE GP  P++   +IEI KGS+ KYELDKK+GL+ +DRVLYS   YP +YG IPRT  ED+DP D+++IM+EPV P   + A+ IGL  MID G+KD K++AV  +DP ++ + DI ++P
Sbjct:    2 NPFHDLEPGPDVPEVVYAIIEIPKGSRNKYELDKKTGLLKLDRVLYSPFFYPVDYGIIPRTWYEDDDPFDIMVIMREPVYPLTIIEARPIGLFKMIDSGDKDYKVLAVPVEDPYFKDWKDIDDVP 126

HSP 2 Score: 20.7866 bits (42), Expect = 3.008e-34
Identity = 7/20 (35.00%), Postives = 15/20 (75.00%), Query Frame = 2

Query:  710 LAEIRRFFEDYKKMRTRKLL 769
            L EI  FF+ YK+++ ++++
Sbjct:  130 LDEIAHFFKRYKELQGKEII 149

The following BLAST results are available for this feature:

BLAST of EY736074 vs. ExPASy Swiss-Prot

BLAST of EY736074 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 113


Match Name	E-value	Identity	Description
IPYR_MAIZE	5.616e-82	81.13	Soluble inorganic pyrophosphatase OS=Zea mays GN=I...	[more]
IPYR_ORYSJ	5.616e-82	79.25	Soluble inorganic pyrophosphatase OS=Oryza sativa ...	[more]
IPYR_ORYSI	5.616e-82	79.25	Soluble inorganic pyrophosphatase OS=Oryza sativa ...	[more]
IPYR_SOLTU	3.085e-78	88.11	Soluble inorganic pyrophosphatase OS=Solanum tuber...	[more]
IPYR2_ARATH	2.569e-75	80.85	Soluble inorganic pyrophosphatase 2 OS=Arabidopsis...	[more]
IPYR_HORVD	4.388e-74	77.85	Soluble inorganic pyrophosphatase OS=Hordeum vulga...	[more]
IPYR2_CHLRE	2.089e-60	70.77	Soluble inorganic pyrophosphatase 2 OS=Chlamydomon...	[more]
IPYR_LEPIN	5.882e-39	56.80	Inorganic pyrophosphatase OS=Leptospira interrogan...	[more]
IPYR_LEPIC	5.882e-39	56.80	Inorganic pyrophosphatase OS=Leptospira interrogan...	[more]
IPYR_PYRFU	3.008e-34	52.80	Inorganic pyrophosphatase OS=Pyrococcus furiosus G...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-705-009-F09-CT.F Sweet orange fruit, development stadium (6 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY736074 ID=EY736074; Name=EY736074; organism=Citrus sinensis; type=EST; length=854bp
CCACGCGTCCGCCCACGCGTCCGAAGAGAGCGGGAAGGGAACCAGCATCA
GTTCTCTTGCTTTTCTCTACTTAAGCGCCCGTAGAGGAACTTAACTTTAA
TATCTTCGCCATTACTCCTCTCTCTTTTCGAGATCGGTGCGTTTCTTTGT
GGTCTTGAAGCTTCGTTCTCGTTAGGCCCTGTTTGGCTTTCAGTACTTGA
ACTGTTACCGTTATGATGGCTCCACCAATTGAGACTTCAAGCAAAACTCC
TGTCTCCCAACCTCCAGCACATCCTCCGCTTAATGAAAGGATACTTTCTT
CAATGAGCAGGAGGTCTGTTGCTGCACATCCTTGGCATGATCTTGAGATT
GGACCTGGAGCTCCAAAGATTTTCAATTGCGTGATTGAAATTGGGAAAGG
GAGCAAGGTGAAATATGAACTTGACAAAAAATCTGGACTGATCATGGTTG
ATCGTGTTCTTTACTCTTCAGTTGTGTACCCCCATAACTATGGTTTCATA
CCTCGTACTCTTTGCGAAGACAATGATCCCCTTGATGTCCTGATCATCAT
GCAGGAGCCAGTTCTTCCAGGCTGCTTTCTTCGGGCTAAAGCCATCGGAC
TCATGCCTATGATTGATCAGGGTGAGAAAGATGATAAGATCATTGCTGTC
TGTGCTGATGATCCCGAGTACCGACACTACAATGACATTAAGGAGCTTCC
ACACATCGTTTGGCTGAGATCCGNCGCTTCTTTGAAGACTACAAGAAAAT
GAGAACAAGGAAGTTGCTGTAAATGACTTTCTGCCAGCCTCTGATGCCCT
ATANTCAGTCCANCAGTCTATGAACCTCTATGCTGACTATATGGTGGGAG
AGCT