EY735451 | Citrus Genome Database

Overview


Name	EY735451
Unique Name	EY735451
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1054

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY735451_ssr142	microsatellite	EY735451_ssr142:142..155.	BLAST: Citrus ESTs to Prunus persica proteins V1
EY735451_ssr141	microsatellite	EY735451_ssr141:141..152.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig12265	contig	Csv1_Contig12265:14..1067.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA4_ARATH (ATPase 4, plasma membrane-type OS=Arabidopsis thaliana GN=AHA4 PE=1 SV=2)

HSP 1 Score: 93.2041 bits (230), Expect = 1.136e-31
Identity = 60/138 (43.48%), Postives = 66/138 (47.83%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDGLYXXXXXXXXXXXMSPH*PPKMWLVLCMKNLLLYPGSMCRVCFVFILNRWFLVCSSFKIPELPGTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLA 416
            YAVSITIRIVLGFMLLALIWQFDF                                PP M L++ + N                                 GTIMTISKDRVKPSPLPDSWKL+EIF TGV+ G Y+A
Sbjct:  656 YAVSITIRIVLGFMLLALIWQFDF--------------------------------PPFMVLIIAILN--------------------------------DGTIMTISKDRVKPSPLPDSWKLSEIFATGVVFGSYMA 729

HSP 2 Score: 55.0694 bits (131), Expect = 1.136e-31
Identity = 28/50 (56.00%), Postives = 34/50 (68.00%), Query Frame = 1

Query:  418 MMTVIFFWAAYRTDFFPRTFGVSSLHEKGYK*LEKALPQQYTLQVSTIYQ 567
            MMTVIFFW +Y+TDFFPRTFGV++L +  +    K     Y LQVS I Q
Sbjct:  730 MMTVIFFWVSYKTDFFPRTFGVATLEKTAHDDFRKLASAIY-LQVSIISQ 778

HSP 3 Score: 28.1054 bits (61), Expect = 1.136e-31
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 2

Query:  566 RALIFLTRARSWSF 607
            +ALIF+TR+RSWSF
Sbjct:  778 QALIFVTRSRSWSF 791

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA7_ARATH (ATPase 7, plasma membrane-type OS=Arabidopsis thaliana GN=AHA7 PE=2 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 1.153e-25
Identity = 56/138 (40.58%), Postives = 62/138 (44.93%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDGLYXXXXXXXXXXXMSPH*PPKMWLVLCMKNLLLYPGSMCRVCFVFILNRWFLVCSSFKIPELPGTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLA 416
            YAVSITIRIV+GFMLL + W+FDF                                PP M LV+ + N                                 GTIMTISKDRVKPSP PD WKL EIF TGV+LG YLA
Sbjct:  648 YAVSITIRIVMGFMLLCVFWEFDF--------------------------------PPFMVLVIAILN--------------------------------DGTIMTISKDRVKPSPTPDCWKLKEIFATGVVLGAYLA 721

HSP 2 Score: 41.2022 bits (95), Expect = 1.153e-25
Identity = 23/58 (39.66%), Postives = 32/58 (55.17%), Query Frame = 1

Query:  418 MMTVIFFWAAYRTDFFPRTFGVSSLHEKGYK*LEKALPQQYT--------LQVSTIYQ 567
            +MTV+FFWAAY T+FF   F V + ++  +K  +K +             LQVSTI Q
Sbjct:  722 IMTVVFFWAAYETNFFHNIFHVRNFNQHHFKMKDKKVAAHLNEQMASAVYLQVSTISQ 779

HSP 3 Score: 29.6462 bits (65), Expect = 1.153e-25
Identity = 18/44 (40.91%), Postives = 28/44 (63.64%), Query Frame = 2

Query:  566 RALIFLTRARSWSFC*PSWAWLLVLGF---RLWSN*LQFLICWP 688
            +ALIF+TR+RSWSF      +LLV+ F   +L ++ +  +  WP
Sbjct:  779 QALIFVTRSRSWSFV-ERPGFLLVIAFLIAQLVASVISAMANWP 821

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA3_NICPL (Plasma membrane ATPase 3 OS=Nicotiana plumbaginifolia GN=PMA3 PE=1 SV=1)

HSP 1 Score: 103.219 bits (256), Expect = 2.660e-24
Identity = 75/180 (41.67%), Postives = 83/180 (46.11%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDGLYXXXXXXXXXXXMSPH*PPKMWLVLCMKNLLLYPGSMCRVCFVFILNRWFLVCSSFKIPELPGTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLANDDSDFLLGCVPDRFLPTYIWCFESS*KRI*MTGKSLASAIY 542
            YAVSITIRIVLGFMLLALIWQFDF                                PP M L++ + N                                 GTIMTISKDRVKPSPLPDSWKLAEIFTTGV+LGGYLA     F        F P  ++   +  K      + LASAIY
Sbjct:  652 YAVSITIRIVLGFMLLALIWQFDF--------------------------------PPFMVLIIAILN--------------------------------DGTIMTISKDRVKPSPLPDSWKLAEIFTTGVVLGGYLAMMTVIFFWAAYKTNFFPR-VFGVSTLEKTATDDFRKLASAIY 766

HSP 2 Score: 28.1054 bits (61), Expect = 2.660e-24
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 2

Query:  566 RALIFLTRARSWSF 607
            +ALIF+TR+RSWSF
Sbjct:  774 QALIFVTRSRSWSF 787

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA1_NICPL (Plasma membrane ATPase 1 OS=Nicotiana plumbaginifolia GN=PMA1 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.660e-24
Identity = 73/180 (40.56%), Postives = 84/180 (46.67%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDGLYXXXXXXXXXXXMSPH*PPKMWLVLCMKNLLLYPGSMCRVCFVFILNRWFLVCSSFKIPELPGTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLANDDSDFLLGCVPDRFLPTYIWCFESS*KRI*MTGKSLASAIY 542
            YAVSITIRIVLGFMLLALIW+FDF                                PP M L++ + N                                 GTIMTISKDRVKPSPLPDSWKLAEIFTTG++LGGYLA     F        F P +++   +  K      + LASAIY
Sbjct:  653 YAVSITIRIVLGFMLLALIWKFDF--------------------------------PPFMVLIIAILN--------------------------------DGTIMTISKDRVKPSPLPDSWKLAEIFTTGIVLGGYLAMMTVIFFWAAYKTNFFP-HVFGVSTLEKTATDDFRKLASAIY 767

HSP 2 Score: 28.8758 bits (63), Expect = 2.660e-24
Identity = 15/27 (55.56%), Postives = 20/27 (74.07%), Query Frame = 2

Query:  566 RALIFLTRARSWSFC*PSWAWLLVLGF 646
            +ALIF+TR+RSWSF      +LLV+ F
Sbjct:  775 QALIFVTRSRSWSFV-ERPGFLLVIAF 800

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA1_SOLLC (Plasma membrane ATPase 1 OS=Solanum lycopersicum GN=LHA1 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 5.860e-24
Identity = 75/180 (41.67%), Postives = 83/180 (46.11%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDGLYXXXXXXXXXXXMSPH*PPKMWLVLCMKNLLLYPGSMCRVCFVFILNRWFLVCSSFKIPELPGTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLANDDSDFLLGCVPDRFLPTYIWCFESS*KRI*MTGKSLASAIY 542
            YAVSITIRIVLGFMLLALIW+FDF                                PP M L++ + N                                 GTIMTISKDRVKPSPLPDSWKLAEIFTTGV+LGGYLA     F        F P  I+   +  K      + LASAIY
Sbjct:  652 YAVSITIRIVLGFMLLALIWKFDF--------------------------------PPFMVLIIAILN--------------------------------DGTIMTISKDRVKPSPLPDSWKLAEIFTTGVVLGGYLAMMTVIFFWAAYKTNFFPR-IFGVSTLEKTATDDFRKLASAIY 766

HSP 2 Score: 28.1054 bits (61), Expect = 5.860e-24
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 2

Query:  566 RALIFLTRARSWSF 607
            +ALIF+TR+RSWSF
Sbjct:  774 QALIFVTRSRSWSF 787

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA2_SOLLC (Plasma membrane ATPase 2 (Fragment) OS=Solanum lycopersicum GN=LHA2 PE=3 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 1.297e-23
Identity = 67/155 (43.23%), Postives = 73/155 (47.10%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDGLYXXXXXXXXXXXMSPH*PPKMWLVLCMKNLLLYPGSMCRVCFVFILNRWFLVCSSFKIPELPGTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLANDDSDFLLGCVPDRFLP 467
            YAVSITIRIVLGFMLLALIW+FDF                                PP M L++ + N                                 GTIMTISKDRVKPSPLPDSWKLAEIFTTGV+LGGYLA     F       +F P
Sbjct:  400 YAVSITIRIVLGFMLLALIWKFDF--------------------------------PPFMVLIIAILN--------------------------------DGTIMTISKDRVKPSPLPDSWKLAEIFTTGVVLGGYLAMMTVIFFWAAYETQFFP 490

HSP 2 Score: 28.1054 bits (61), Expect = 1.297e-23
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 2

Query:  566 RALIFLTRARSWSF 607
            +ALIF+TR+RSWSF
Sbjct:  522 QALIFVTRSRSWSF 535

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA2_ARATH (ATPase 2, plasma membrane-type OS=Arabidopsis thaliana GN=AHA2 PE=1 SV=2)

HSP 1 Score: 73.9442 bits (180), Expect = 1.620e-23
Identity = 44/80 (55.00%), Postives = 52/80 (65.00%), Query Frame = 3

Query:  198 MKNLLLYPGSMC-RVCFVFILNR--WFLVCSSFKIPELP----GTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLA 416
            MKN  +Y  S+  R+ F F+L    W    S+F +  +     GTIMTISKDRVKPSP PDSWKL EIF TGV+LGGY A
Sbjct:  642 MKNYTIYAVSITIRIVFGFMLIALIWEFDFSAFMVLIIAILNDGTIMTISKDRVKPSPTPDSWKLKEIFATGVVLGGYQA 721

HSP 2 Score: 46.2098 bits (108), Expect = 1.620e-23
Identity = 25/50 (50.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query:  418 MMTVIFFWAAYRTDFFPRTFGVSSLHEKGYK*LEKALPQQYTLQVSTIYQ 567
            +MTVIFFWAA++TDFF  TFGV S+ +  ++     L     LQVS I Q
Sbjct:  722 IMTVIFFWAAHKTDFFSDTFGVRSIRDNNHE-----LMGAVYLQVSIISQ 766

HSP 3 Score: 28.4906 bits (62), Expect = 1.620e-23
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 2

Query:  566 RALIFLTRARSWSFC*PSWAWLLV 637
            +ALIF+TR+RSWSF     A L++
Sbjct:  766 QALIFVTRSRSWSFVERPGALLMI 789

HSP 4 Score: 68.5514 bits (166), Expect = 7.177e-11
Identity = 33/38 (86.84%), Postives = 35/38 (92.11%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDG 116
            YAVSITIRIV GFML+ALIW+FDF  FMVLIIAILNDG
Sbjct:  648 YAVSITIRIVFGFMLIALIWEFDFSAFMVLIIAILNDG 685

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA4_NICPL (Plasma membrane ATPase 4 OS=Nicotiana plumbaginifolia GN=PMA4 PE=2 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 4.749e-22
Identity = 44/80 (55.00%), Postives = 53/80 (66.25%), Query Frame = 3

Query:  198 MKNLLLYPGSMC-RVCF--VFILNRWFLVCSSFKIPELP----GTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLA 416
            MKN  +Y  S+  R+ F  +FI   W    S+F +  +     GTIMTISKDRVKPSP+PDSWKL EIF TGV+LGGY A
Sbjct:  645 MKNYTIYAVSITIRIVFGFMFIALIWKYDFSAFMVLIIAILNDGTIMTISKDRVKPSPMPDSWKLKEIFATGVVLGGYQA 724

HSP 2 Score: 40.4318 bits (93), Expect = 4.749e-22
Identity = 22/50 (44.00%), Postives = 28/50 (56.00%), Query Frame = 1

Query:  418 MMTVIFFWAAYRTDFFPRTFGVSSLHEKGYK*LEKALPQQYTLQVSTIYQ 567
            +MTV+FFWA + TDFF   FGV SL        ++ +     LQVS I Q
Sbjct:  725 LMTVVFFWAMHDTDFFSDKFGVKSLRNS-----DEEMMSALYLQVSIISQ 769

HSP 3 Score: 28.4906 bits (62), Expect = 4.749e-22
Identity = 15/29 (51.72%), Postives = 20/29 (68.97%), Query Frame = 2

Query:  566 RALIFLTRARSWSFC*PSWAWLLVLGFRL 652
            +ALIF+TR+RSWSF       LLV+ F +
Sbjct:  769 QALIFVTRSRSWSFL-ERPGMLLVIAFMI 796

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA1_ARATH (ATPase 1, plasma membrane-type OS=Arabidopsis thaliana GN=AHA1 PE=1 SV=3)

HSP 1 Score: 73.559 bits (179), Expect = 7.983e-22
Identity = 43/80 (53.75%), Postives = 52/80 (65.00%), Query Frame = 3

Query:  198 MKNLLLYPGSMC-RVCFVFILNR--WFLVCSSFKIPELP----GTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLA 416
            MKN  +Y  S+  R+ F F+L    W    S+F +  +     GTIMTISKDRVKPSP PDSWKL EIF TG++LGGY A
Sbjct:  642 MKNYTIYAVSITIRIVFGFMLIALIWEFDFSAFMVLIIAILNDGTIMTISKDRVKPSPTPDSWKLKEIFATGIVLGGYQA 721

HSP 2 Score: 40.817 bits (94), Expect = 7.983e-22
Identity = 23/50 (46.00%), Postives = 32/50 (64.00%), Query Frame = 1

Query:  418 MMTVIFFWAAYRTDFFPRTFGVSSLHEKGYK*LEKALPQQYTLQVSTIYQ 567
            +M+VIFFWAA++TDFF   FGV S+ +      ++ +   Y LQVS I Q
Sbjct:  722 IMSVIFFWAAHKTDFFSDKFGVRSIRDNN----DELMGAVY-LQVSIISQ 766

HSP 3 Score: 28.4906 bits (62), Expect = 7.983e-22
Identity = 13/24 (54.17%), Postives = 18/24 (75.00%), Query Frame = 2

Query:  566 RALIFLTRARSWSFC*PSWAWLLV 637
            +ALIF+TR+RSWSF     A L++
Sbjct:  766 QALIFVTRSRSWSFVERPGALLMI 789

HSP 4 Score: 68.5514 bits (166), Expect = 7.177e-11
Identity = 33/38 (86.84%), Postives = 35/38 (92.11%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDG 116
            YAVSITIRIV GFML+ALIW+FDF  FMVLIIAILNDG
Sbjct:  648 YAVSITIRIVFGFMLIALIWEFDFSAFMVLIIAILNDG 685

[Close]

BLAST of EY735451 vs. ExPASy Swiss-Prot
Match: PMA3_ARATH (ATPase 3, plasma membrane-type OS=Arabidopsis thaliana GN=AHA3 PE=1 SV=2)

HSP 1 Score: 74.7146 bits (182), Expect = 1.132e-21
Identity = 44/80 (55.00%), Postives = 52/80 (65.00%), Query Frame = 3

Query:  198 MKNLLLYPGSMC-RVCFVFILNR--WFLVCSSFKIPELP----GTIMTISKDRVKPSPLPDSWKLAEIFTTGVILGGYLA 416
            MKN  +Y  S+  R+ F F+L    W    S F +  +     GTIMTISKDRVKPSP PDSWKL EIF TGV+LGGY+A
Sbjct:  643 MKNYTIYAVSITIRIVFGFMLIALIWKFDFSPFMVLIIAILNDGTIMTISKDRVKPSPTPDSWKLKEIFATGVVLGGYMA 722

HSP 2 Score: 47.7506 bits (112), Expect = 1.132e-21
Identity = 25/50 (50.00%), Postives = 30/50 (60.00%), Query Frame = 1

Query:  418 MMTVIFFWAAYRTDFFPRTFGVSSLHEKGYK*LEKALPQQYTLQVSTIYQ 567
            +MTV+FFWAAY+TDFFPRTF V  L        E  +     LQVS + Q
Sbjct:  723 IMTVVFFWAAYKTDFFPRTFHVRDLRGS-----EHEMMSALYLQVSIVSQ 767

HSP 3 Score: 71.2478 bits (173), Expect = 1.107e-11
Identity = 34/38 (89.47%), Postives = 36/38 (94.74%), Query Frame = 3

Query:    3 YAVSITIRIVLGFMLLALIWQFDFPPFMVLIIAILNDG 116
            YAVSITIRIV GFML+ALIW+FDF PFMVLIIAILNDG
Sbjct:  649 YAVSITIRIVFGFMLIALIWKFDFSPFMVLIIAILNDG 686

The following BLAST results are available for this feature:

BLAST of EY735451 vs. ExPASy Swiss-Prot

BLAST of EY735451 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 19


Match Name	E-value	Identity	Description
PMA4_ARATH	1.136e-31	43.48	ATPase 4, plasma membrane-type OS=Arabidopsis thal...	[more]
PMA7_ARATH	1.153e-25	40.58	ATPase 7, plasma membrane-type OS=Arabidopsis thal...	[more]
PMA3_NICPL	2.660e-24	41.67	Plasma membrane ATPase 3 OS=Nicotiana plumbaginifo...	[more]
PMA1_NICPL	2.660e-24	40.56	Plasma membrane ATPase 1 OS=Nicotiana plumbaginifo...	[more]
PMA1_SOLLC	5.860e-24	41.67	Plasma membrane ATPase 1 OS=Solanum lycopersicum G...	[more]
PMA2_SOLLC	1.297e-23	43.23	Plasma membrane ATPase 2 (Fragment) OS=Solanum lyc...	[more]
PMA2_ARATH	1.620e-23	55.00	ATPase 2, plasma membrane-type OS=Arabidopsis thal...	[more]
PMA4_NICPL	4.749e-22	55.00	Plasma membrane ATPase 4 OS=Nicotiana plumbaginifo...	[more]
PMA1_ARATH	7.983e-22	53.75	ATPase 1, plasma membrane-type OS=Arabidopsis thal...	[more]
PMA3_ARATH	1.132e-21	55.00	ATPase 3, plasma membrane-type OS=Arabidopsis thal...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-704-109-C08-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY735451 ID=EY735451; Name=EY735451; organism=Citrus sinensis; type=EST; length=1054bp
TATATGCAGTATCTATTACCATCCGTATTGTGCTTGGTTTCATGCTGCTG
GCTCTCATTTGGCAGTTTGACTTCCCACCCTTCATGGTGCTGATAATTGC
TATTCTTAATGATGGTTTGTATTGCTCTTTCTTTTTTTTTTGTGTGTGTG
TGTGTATGTCTCCCCACTGACCCCCCAAAATGTGGTTGGTTCTCTGTATG
AAGAATTTATTACTTTATCCTGGTTCCATGTGTCGAGTTTGTTTTGTCTT
TATCCTGAATAGATGGTTCCTTGTCTGTTCTTCCTTCAAAATACCTGAAC
TGCCAGGTACCATTATGACAATATCGAAAGATAGAGTGAAACCATCTCCT
CTGCCAGACAGCTGGAAATTGGCTGAGATCTTTACAACTGGAGTCATTCT
TGGTGGTTATTTAGCAAATGATGACAGTGATTTTCTTCTGGGCTGCGTAC
CGGACAGATTTCTTCCCACGTACATTTGGTGTTTCGAGTCTTCATGAAAA
AGGATATAAATGACTGGAAAAAGCCTTGCCTCAGCAATATACCCTGCAAG
TGAGTACCATTTATCAGGGCCCTCATATTCTTGACCCGTGCTAGGAGTTG
GTCTTTTTGTTGACCGTCCTGGGCCTGGTTACTTGTTCTGGGTTTTCGCT
TGTGGTCCAACTAATTGCAATTCCTGATTTGCTGGCCAATGCAAACGTGG
AACTTTTTCTGAAATTTAAAGGATTTGGGATGGGGGTTGGGGTTGGTGGG
GGTCCGGCTTTTACAAATCCACCTTTTTACCTCCCCACTTTGATTTTTTT
AATATCTTCCCATCCAAAATCGCTTTGAACGGAGAAAGGGGTTGAAATTT
TTTCCTTTTTAGTAACAAGGGATATTTCCTTTCTACGGAAAAAAAAAAGT
TTTTGAGGTAAAAAAAAGAAATGAAGTGTATTTTATTAGGGCCATATTCA
ATAACATAAAAGACTCCCCCCACCCTCCGGGTGTTATATACCTACACACC
TATTGTAATAATAAAACGGAATATATTCTTCATGACTAGAAATAAACGCA
AAAC