EY756182

Overview
NameEY756182
Unique NameEY756182
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length619
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12994 contig Csv1_Contig12994:407..1025. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_BRANA (10 kDa chaperonin OS=Brassica napus PE=3 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 2.051e-36
Identity = 71/97 (73.20%), Postives = 85/97 (87.63%), Query Frame = 2
Query:   59 MAKRLIPAFSRVLVEKIIPPSKTNSGILLPEKTSKLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            M KRLIP F+R+LV+ +I P+KT SGILLPEK SKL SGKV+AVGPG+RD +GK IPVSVKEGDTVLLPEYGG +VKLG+K+YHL+ DE +LGTLH+
Sbjct:    1 MMKRLIPTFNRILVQGVIQPAKTESGILLPEKASKLNSGKVIAVGPGSRDKDGKLIPVSVKEGDTVLLPEYGGTQVKLGEKEYHLFRDEDVLGTLHE 97          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_ARATH (10 kDa chaperonin OS=Arabidopsis thaliana GN=CPN10 PE=2 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 2.051e-36
Identity = 70/97 (72.16%), Postives = 86/97 (88.66%), Query Frame = 2
Query:   59 MAKRLIPAFSRVLVEKIIPPSKTNSGILLPEKTSKLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            M KRLIP F+R+LV+++I P+KT SGILLPEK+SKL SGKV+AVGPG+RD +GK IPVSVKEGDTVLLPEYGG +VKLG+ +YHL+ DE +LGTLH+
Sbjct:    1 MMKRLIPTFNRILVQRVIQPAKTESGILLPEKSSKLNSGKVIAVGPGSRDKDGKLIPVSVKEGDTVLLPEYGGTQVKLGENEYHLFRDEDVLGTLHE 97          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_ORYLA (10 kDa heat shock protein, mitochondrial OS=Oryzias latipes GN=hspe1 PE=3 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 2.519e-18
Identity = 46/96 (47.92%), Postives = 65/96 (67.71%), Query Frame = 2
Query:   65 KRLIPAFSRVLVEKIIPPSKTNSGILLPEKTS-KLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            ++ +P F RVLVE+++  + T  GI+LPEK+  K++   VVAVGPG+ +  G+  P+SVK G+ VLLP+YGG +V L DK Y L+ D  ILG   D
Sbjct:    4 RKFLPLFDRVLVERLMAETVTKGGIMLPEKSQGKVLQATVVAVGPGSMNQKGEVQPMSVKVGEKVLLPQYGGTKVVLEDKDYFLFRDADILGKYVD 99          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_MOUSE (10 kDa heat shock protein, mitochondrial OS=Mus musculus GN=Hspe1 PE=1 SV=2)

HSP 1 Score: 92.0485 bits (227), Expect = 2.519e-18
Identity = 48/96 (50.00%), Postives = 62/96 (64.58%), Query Frame = 2
Query:   65 KRLIPAFSRVLVEKIIPPSKTNSGILLPEKTS-KLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            ++ +P F RVLVE+    + T  GI+LPEK+  K++   VVAVG G +  +G+  PVSVK GD VLLPEYGG +V L DK Y L+ D  ILG   D
Sbjct:    7 RKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGGKGKSGEIEPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDSDILGKYVD 102          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_HUMAN (10 kDa heat shock protein, mitochondrial OS=Homo sapiens GN=HSPE1 PE=1 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 4.297e-18
Identity = 48/96 (50.00%), Postives = 62/96 (64.58%), Query Frame = 2
Query:   65 KRLIPAFSRVLVEKIIPPSKTNSGILLPEKTS-KLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            ++ +P F RVLVE+    + T  GI+LPEK+  K++   VVAVG G++   G+  PVSVK GD VLLPEYGG +V L DK Y L+ D  ILG   D
Sbjct:    7 RKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD 102          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_BOVIN (10 kDa heat shock protein, mitochondrial OS=Bos taurus GN=HSPE1 PE=2 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 4.297e-18
Identity = 48/96 (50.00%), Postives = 62/96 (64.58%), Query Frame = 2
Query:   65 KRLIPAFSRVLVEKIIPPSKTNSGILLPEKTS-KLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            ++ +P F RVLVE+    + T  GI+LPEK+  K++   VVAVG G++   G+  PVSVK GD VLLPEYGG +V L DK Y L+ D  ILG   D
Sbjct:    7 RKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGSKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD 102          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_RAT (10 kDa heat shock protein, mitochondrial OS=Rattus norvegicus GN=Hspe1 PE=1 SV=3)

HSP 1 Score: 90.8929 bits (224), Expect = 5.611e-18
Identity = 48/96 (50.00%), Postives = 61/96 (63.54%), Query Frame = 2
Query:   65 KRLIPAFSRVLVEKIIPPSKTNSGILLPEKTS-KLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            ++ +P F RVLVE+    + T  GI+LPEK+  K++   VVAVG G +   G+  PVSVK GD VLLPEYGG +V L DK Y L+ D  ILG   D
Sbjct:    7 RKFLPLFDRVLVERSAAETVTKGGIMLPEKSQGKVLQATVVAVGSGGKGKGGEIQPVSVKVGDKVLLPEYGGTKVVLDDKDYFLFRDGDILGKYVD 102          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_SCHPO (10 kDa heat shock protein, mitochondrial OS=Schizosaccharomyces pombe GN=hsp10 PE=1 SV=1)

HSP 1 Score: 89.7373 bits (221), Expect = 1.250e-17
Identity = 44/99 (44.44%), Postives = 64/99 (64.65%), Query Frame = 2
Query:   56 KMAKRLIPAFSRVLVEKIIPPSKTNSGILLPEKT-SKLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESILGTLHD 349
            K AK ++P   R+LV++I   +KT SGI LPEK+  KL  G+V++VG G  +  GK    SV  GD VLLP YGG+ +K+G+++Y LY D  +L  + +
Sbjct:    6 KSAKSIVPLLDRILVQRIKADTKTASGIFLPEKSVEKLSEGRVISVGKGGYNKEGKLAQPSVAVGDRVLLPAYGGSNIKVGEEEYSLYRDHELLAIIKE 104          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_YEAST (10 kDa heat shock protein, mitochondrial OS=Saccharomyces cerevisiae GN=HSP10 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.860e-16
Identity = 46/98 (46.94%), Postives = 62/98 (63.27%), Query Frame = 2
Query:   56 KMAKRLIPAFSRVLVEKIIPPSKTNSGILLPEKT-SKLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLG-DKKYHLYEDESILGTL 343
            K AK ++P   RVLV++I   +KT SG+ LPEK   KL   +VVAVGPG  D NG  +   VK GD VL+P++GG+ +KLG D +  L+ D  IL  +
Sbjct:    6 KSAKSIVPLMDRVLVQRIKAQAKTASGLYLPEKNVEKLNQAEVVAVGPGFTDANGNKVVPQVKVGDQVLIPQFGGSTIKLGNDDEVILFRDAEILAKI 103          
BLAST of EY756182 vs. ExPASy Swiss-Prot
Match: CH10_SCHJA (10 kDa heat shock protein, mitochondrial OS=Schistosoma japonicum GN=SJCHGC01960 PE=3 SV=2)

HSP 1 Score: 81.2629 bits (199), Expect = 4.446e-15
Identity = 40/91 (43.96%), Postives = 57/91 (62.64%), Query Frame = 2
Query:   65 KRLIPAFSRVLVEKIIPPSKTNSGILLPEKTS-KLISGKVVAVGPGARDVNGKFIPVSVKEGDTVLLPEYGGAEVKLGDKKYHLYEDESIL 334
            ++  P F RVLV++    +K+  GI+LPEK   K++   VVA GPG ++  G+ +PV V  GD V LPEYGG +V L D +Y L+ +  IL
Sbjct:    7 RKFAPLFDRVLVQRFEAETKSKGGIMLPEKAKGKVLEATVVAHGPGVKNEKGEVVPVCVTVGDKVFLPEYGGTKVVLEDTEYFLFRESDIL 97          
The following BLAST results are available for this feature:
BLAST of EY756182 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 62
Match NameE-valueIdentityDescription
CH10_BRANA2.051e-3673.2010 kDa chaperonin OS=Brassica napus PE=3 SV=1[more]
CH10_ARATH2.051e-3672.1610 kDa chaperonin OS=Arabidopsis thaliana GN=CPN10... [more]
CH10_ORYLA2.519e-1847.9210 kDa heat shock protein, mitochondrial OS=Oryzia... [more]
CH10_MOUSE2.519e-1850.0010 kDa heat shock protein, mitochondrial OS=Mus mu... [more]
CH10_HUMAN4.297e-1850.0010 kDa heat shock protein, mitochondrial OS=Homo s... [more]
CH10_BOVIN4.297e-1850.0010 kDa heat shock protein, mitochondrial OS=Bos ta... [more]
CH10_RAT5.611e-1850.0010 kDa heat shock protein, mitochondrial OS=Rattus... [more]
CH10_SCHPO1.250e-1744.4410 kDa heat shock protein, mitochondrial OS=Schizo... [more]
CH10_YEAST6.860e-1646.9410 kDa heat shock protein, mitochondrial OS=Saccha... [more]
CH10_SCHJA4.446e-1543.9610 kDa heat shock protein, mitochondrial OS=Schist... [more]

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Libraries
Library NameType
Sweet orange leaf, field plant Bcdna_library
Properties
Property NameValue
Genbank descriptionCS13-C1-001-005-F01-CT.F Sweet orange leaf, field plant B Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY756182 ID=EY756182|Name=EY756182|organism=Citrus sinensis|type=EST|length=619bp
CCACGCGTCCGGAAGAAAGTTTTGGTGAAGTCCCCTCCTGTTTGTTCGTG
AAGAGAAAATGGCAAAGAGATTGATTCCAGCATTCAGTCGCGTTTTGGTT
GAAAAAATAATCCCTCCTTCCAAAACCAACTCCGGAATCTTACTTCCTGA
GAAAACAAGCAAGCTGATTTCTGGAAAAGTTGTTGCTGTGGGTCCTGGAG
CTCGTGATGTCAATGGAAAGTTTATTCCTGTAAGTGTGAAGGAAGGAGAT
ACTGTTCTTTTGCCCGAGTATGGGGGGGCTGAAGTTAAGCTTGGTGATAA
AAAGTACCATCTGTACGAGGATGAGTCCATCCTGGGAACACTTCATGACC
ATTGAAAAATATTGCTCTTTGATTGCATCTAGTAATGGCAGCTTTAATAA
TTCAAAGTTGTCGGCTGGAGCAGCCTTGTGACTGAATATTAGTGCTATAT
ACATTTAAACTTATTTAGGACTTGAGAAGTGTTTTCATATAACTTGATGT
ACTAGTTCAGAAACTAATTTGCGACTGAGAATTGCTAAGAATTGCTAAGG
GCTGCAGTTTATAGCGCATTATTCGAAGTGNNCCTTGGTCAAAAAAAAAA
AAAAAAAAAAAAAAAAAAA
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