Study on genetic diversity of 39 lemon, lime, and Rangpur germplasm resources with scot and ISSR markers

The genetic relationships and genetic diversity of 39 lemon (Citrus limon), lime (C. aurantifolia), and Rangpur (C. limonia) germplasm resources were examined by SCoT and ISSR markers in this study. Of the 80 SCoT primers screened, 9 primers gave reproducible, polymorphic DNA amplification patterns with a total 69 of amplified bands, and the percentage of polymorphic bands was 76.8%. Of the 100 ISSR primers screened, 9 primers gave reproducible, polymorphic DNA amplification patterns with a total 84 of amplified bands, and the percentage of polymorphic bands was 77.4%. UPGMA relationship trees were established based on their similarity coefficients. At the coefficient level of 0.85 for SCoT and 0.77 for ISSR, the 39 germplasm accessions were divided into six groups and five groups, respectively. The application of SCoT and ISSR markers is discussed in this paper.