EY755663

Overview
NameEY755663
Unique NameEY755663
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length896
Libraries
Library NameType
Sweet orange leaf, field plant Acdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig10887 contig Csv1_Contig10887:97..995. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_TOBAC (Proteasome subunit alpha type-6 OS=Nicotiana tabacum GN=PAA1 PE=2 SV=1)

HSP 1 Score: 362.073 bits (928), Expect = 2.485e-99
Identity = 187/246 (76.02%), Postives = 202/246 (82.11%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKKIXXXXXXXXXXXXXXSXI-XVLSIEEIDEHLTALSERD 795
            MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAG+TSIGVRGKDSVCVVTQKKVPDKLLD TSV+HLFPITKYLGLLATGMTADARTLVQQAR EAAEFRFKYGYEMPVDVL+KWIADKSQVYTQHAYMRPLGVVAM+L IDEE GP+LFKCDPAGHFFGHKATSAG KEQEAINFLEK+ +      +++  +          ++                 I  VL+ EEIDEHLTA+SERD
Sbjct:    1 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKLLDQTSVSHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRFKYGYEMPVDVLSKWIADKSQVYTQHAYMRPLGVVAMILGIDEEKGPQLFKCDPAGHFFGHKATSAGSKEQEAINFLEKKMKNDPAFSYEETVQTAISALQSVLQEDFKASEIEVGVVKKEDPIFRVLTTEEIDEHLTAISERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_SOYBN (Proteasome subunit alpha type-6 OS=Glycine max GN=PAA1 PE=2 SV=2)

HSP 1 Score: 355.14 bits (910), Expect = 3.037e-97
Identity = 181/246 (73.58%), Postives = 201/246 (81.71%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKK-IXXXXXXXXXXXXXXSXIXVLSIEEIDEHLTALSERD 795
            MSRGSGGGY+RHITIFSPEGRLFQVEYAFKAVKAAG+TSIGVRGKDS+CVVT KKVPDKLLD+TSVTHLFPITKYLGLLATGMTADARTLVQQAR EAAEFRF YGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVL ID+E GP+L+KCDPAGH+FGHKATSAGLK+QEAINFLEK+ +      +++  +          ++                    VL+ +EIDEHLTA+SERD
Sbjct:    1 MSRGSGGGYNRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSICVVTHKKVPDKLLDNTSVTHLFPITKYLGLLATGMTADARTLVQQARNEAAEFRFTYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLGIDDEYGPQLYKCDPAGHYFGHKATSAGLKDQEAINFLEKKMKNDPSFTYEETVQTAISALQSVLQEDFKATEIEVGVVRKDNPEFRVLTTDEIDEHLTAISERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_ORYSJ (Proteasome subunit alpha type-6 OS=Oryza sativa subsp. japonica GN=PAA1 PE=2 SV=1)

HSP 1 Score: 347.436 bits (890), Expect = 6.333e-95
Identity = 177/246 (71.95%), Postives = 200/246 (81.30%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKKIXXXXXXXXXXXXXXSXI-XVLSIEEIDEHLTALSERD 795
            MSRG+G GYDRHITIFSPEGRL+QVEYAFKAVK+AGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKY+GLLATG+TADAR+LV QAR EAAEFRFK+GYEMPVDVLAKWIADK+QVYTQHAYMRPLGVVAMVL  DEE   +LFKCDPAGHFFGHKATSAGLKEQEAINFLEK+ +   +  +++  ++         ++                 +   L+ EEID+HLTA+SERD
Sbjct:    1 MSRGTGAGYDRHITIFSPEGRLYQVEYAFKAVKSAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYIGLLATGLTADARSLVYQARNEAAEFRFKWGYEMPVDVLAKWIADKAQVYTQHAYMRPLGVVAMVLGYDEEKNAQLFKCDPAGHFFGHKATSAGLKEQEAINFLEKKMKDDPQFSYEETVQIAISALQSVLQEDFKATEIEVGVVRKDDRVFRALTTEEIDQHLTAISERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6B_ARATH (Proteasome subunit alpha type-6-B OS=Arabidopsis thaliana GN=PAA2 PE=1 SV=1)

HSP 1 Score: 346.28 bits (887), Expect = 1.411e-94
Identity = 176/246 (71.54%), Postives = 196/246 (79.67%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKK-IXXXXXXXXXXXXXXSXIXVLSIEEIDEHLTALSERD 795
            MSRGSG GYDRHITIFSPEGRLFQVEYAFKAVKAAG+TSIGVRGKDSVCVVTQKKVPDKLLD +SV+HLFP+TKYLGLLATGMTAD+R+LVQQAR EAAEFRF+YGYEMP D+LAKWIADKSQVYTQHAYMRPLGVVAMVL IDEE GP L+KCDPAGHF+GHKATSAG+KEQEA+NFLEK+ +      + +  +          ++                     L  EEIDEHLTA+SERD
Sbjct:    1 MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKAAGITSIGVRGKDSVCVVTQKKVPDKLLDQSSVSHLFPVTKYLGLLATGMTADSRSLVQQARNEAAEFRFQYGYEMPADILAKWIADKSQVYTQHAYMRPLGVVAMVLGIDEERGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKENPAFTYDETVQTAISALQSVLQEDFKATEIEVGVVRADDPLFRSLRTEEIDEHLTAISERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6A_ARATH (Proteasome subunit alpha type-6-A OS=Arabidopsis thaliana GN=PAA1 PE=1 SV=2)

HSP 1 Score: 343.969 bits (881), Expect = 7.002e-94
Identity = 173/246 (70.33%), Postives = 196/246 (79.67%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKK-IXXXXXXXXXXXXXXSXIXVLSIEEIDEHLTALSERD 795
            MSRGSG GYDRHITIFSPEGRLFQVEYAFKAVK AG+TSIGVRGKDSVCVVTQKKVPDKLLD +SVTHLFPITKY+GL+ATG+TADAR+LVQQAR +AAEFRF YGYEMPVD+LAKWIADKSQVYTQHAYMRPLGVVAMV+ +DEE GP L+KCDPAGHF+GHKATSAG+KEQEA+NFLEK+ +      F +  +          ++                     L+ EEI+EHLTA+SERD
Sbjct:    1 MSRGSGAGYDRHITIFSPEGRLFQVEYAFKAVKTAGITSIGVRGKDSVCVVTQKKVPDKLLDQSSVTHLFPITKYIGLVATGITADARSLVQQARNQAAEFRFTYGYEMPVDILAKWIADKSQVYTQHAYMRPLGVVAMVMGVDEENGPLLYKCDPAGHFYGHKATSAGMKEQEAVNFLEKKMKENPSFTFDETVQTAISALQSVLQEDFKATEIEVGVVRAENPEFRALTTEEIEEHLTAISERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_RAT (Proteasome subunit alpha type-6 OS=Rattus norvegicus GN=Psma6 PE=1 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 6.599e-68
Identity = 133/247 (53.85%), Postives = 174/247 (70.45%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKKIXXXXXXXXXXXXXXS--XIXVLSIEEIDEHLTALSERD 795
            MSRGS  G+DRHITIFSPEGRL+QVEYAFKA+   G+TS+ VRGKD   +VTQKKVPDKLLD ++VTHLF IT+ +G + TGMTAD+R+ VQ+ARYEAA +++KYGYE+PVD+L K IAD SQVYTQ+A MRPLG   +++ IDEE GP+++KCDPAG++ G KAT+AG+K+ E+ +FLEK+ +      F++        C  +   I                    +L+  EID HL AL+ERD
Sbjct:    1 MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAI-TCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_MOUSE (Proteasome subunit alpha type-6 OS=Mus musculus GN=Psma6 PE=1 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 6.599e-68
Identity = 133/247 (53.85%), Postives = 174/247 (70.45%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKKIXXXXXXXXXXXXXXS--XIXVLSIEEIDEHLTALSERD 795
            MSRGS  G+DRHITIFSPEGRL+QVEYAFKA+   G+TS+ VRGKD   +VTQKKVPDKLLD ++VTHLF IT+ +G + TGMTAD+R+ VQ+ARYEAA +++KYGYE+PVD+L K IAD SQVYTQ+A MRPLG   +++ IDEE GP+++KCDPAG++ G KAT+AG+K+ E+ +FLEK+ +      F++        C  +   I                    +L+  EID HL AL+ERD
Sbjct:    1 MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITESIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAI-TCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_HUMAN (Proteasome subunit alpha type-6 OS=Homo sapiens GN=PSMA6 PE=1 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 6.599e-68
Identity = 133/247 (53.85%), Postives = 174/247 (70.45%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKKIXXXXXXXXXXXXXXS--XIXVLSIEEIDEHLTALSERD 795
            MSRGS  G+DRHITIFSPEGRL+QVEYAFKA+   G+TS+ VRGKD   +VTQKKVPDKLLD ++VTHLF IT+ +G + TGMTAD+R+ VQ+ARYEAA +++KYGYE+PVD+L K IAD SQVYTQ+A MRPLG   +++ IDEE GP+++KCDPAG++ G KAT+AG+K+ E+ +FLEK+ +      F++        C  +   I                    +L+  EID HL AL+ERD
Sbjct:    1 MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAI-TCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_BOVIN (Proteasome subunit alpha type-6 OS=Bos taurus GN=PSMA6 PE=1 SV=1)

HSP 1 Score: 257.684 bits (657), Expect = 6.599e-68
Identity = 133/247 (53.85%), Postives = 174/247 (70.45%), Query Frame = 1
Query:   61 MSRGSGGGYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKKLCRLQFPPCNQSCKKIXXXXXXXXXXXXXXS--XIXVLSIEEIDEHLTALSERD 795
            MSRGS  G+DRHITIFSPEGRL+QVEYAFKA+   G+TS+ VRGKD   +VTQKKVPDKLLD ++VTHLF IT+ +G + TGMTAD+R+ VQ+ARYEAA +++KYGYE+PVD+L K IAD SQVYTQ+A MRPLG   +++ IDEE GP+++KCDPAG++ G KAT+AG+K+ E+ +FLEK+ +      F++        C  +   I                    +L+  EID HL AL+ERD
Sbjct:    1 MSRGSSAGFDRHITIFSPEGRLYQVEYAFKAINQGGLTSVAVRGKDCAVIVTQKKVPDKLLDSSTVTHLFKITENIGCVMTGMTADSRSQVQRARYEAANWKYKYGYEIPVDMLCKRIADISQVYTQNAEMRPLGCCMILIGIDEEQGPQVYKCDPAGYYCGFKATAAGVKQTESTSFLEKKVKKKFDWTFEQTVETAI-TCLSTVLSIDFKPSEIEVGVVTVENPKFRILTEAEIDAHLVALAERD 246          
BLAST of EY755663 vs. ExPASy Swiss-Prot
Match: PSA6_SCHPO (Probable proteasome subunit alpha type-6 OS=Schizosaccharomyces pombe GN=SPBC646.16 PE=2 SV=1)

HSP 1 Score: 226.098 bits (575), Expect = 2.128e-58
Identity = 123/247 (49.80%), Postives = 157/247 (63.56%), Query Frame = 1
Query:   82 GYDRHITIFSPEGRLFQVEYAFKAVKAAGVTSIGVRGKDSVCVVTQKKVPDKLLDHTSVTHLFPITKYLGLLATGMTADARTLVQQARYEAAEFRFKYGYEMPVDVLAKWIADKSQVYTQHAYMRPLGVVAMVLSIDEECGPRLFKCDPAGHFFGHKATSAGLKEQEAINFLEKR*RMILRLPFKK---------LCRLQFPPCNQSCKKIXXXXXXXXXXXXXXSXIXVLSIEEIDEHLTALSERD 795
            G+DR IT+FSPEGRL+QVEYAFKA   AG+TS+GV GK+  CV++QKKVPDKL+D ++V H+FPITK +G + TG  ADAR  V +AR EAAEF +K GY MP DVLAK +A+ +QV TQ A MRPLGV   ++++D+E GP LFK DPAG + G+KATSAG K+ E IN+LEKR        FKK                    S                      VLS+EEI+ HL +++E+D
Sbjct:    6 GFDRTITVFSPEGRLYQVEYAFKAFNNAGITSVGVTGKNCACVISQKKVPDKLIDASTVKHVFPITKGIGCVMTGSIADARAQVSRARSEAAEFEYKNGYPMPCDVLAKRMANIAQVSTQRAAMRPLGVAMTLVAVDDEIGPSLFKLDPAGFYIGYKATSAGPKQTETINWLEKR--------FKKDGIPSNLTDTVETGIQALMSSLSTDFKSTELQIGVVEDDKPFRVLSVEEIEAHLQSIAEKD 244          
The following BLAST results are available for this feature:
BLAST of EY755663 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 175
Match NameE-valueIdentityDescription
PSA6_TOBAC2.485e-9976.02Proteasome subunit alpha type-6 OS=Nicotiana tabac... [more]
PSA6_SOYBN3.037e-9773.58Proteasome subunit alpha type-6 OS=Glycine max GN=... [more]
PSA6_ORYSJ6.333e-9571.95Proteasome subunit alpha type-6 OS=Oryza sativa su... [more]
PSA6B_ARATH1.411e-9471.54Proteasome subunit alpha type-6-B OS=Arabidopsis t... [more]
PSA6A_ARATH7.002e-9470.33Proteasome subunit alpha type-6-A OS=Arabidopsis t... [more]
PSA6_RAT6.599e-6853.85Proteasome subunit alpha type-6 OS=Rattus norvegic... [more]
PSA6_MOUSE6.599e-6853.85Proteasome subunit alpha type-6 OS=Mus musculus GN... [more]
PSA6_HUMAN6.599e-6853.85Proteasome subunit alpha type-6 OS=Homo sapiens GN... [more]
PSA6_BOVIN6.599e-6853.85Proteasome subunit alpha type-6 OS=Bos taurus GN=P... [more]
PSA6_SCHPO2.128e-5849.80Probable proteasome subunit alpha type-6 OS=Schizo... [more]

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Properties
Property NameValue
Genbank descriptionCS12-C1-001-031-B06-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY755663 ID=EY755663; Name=EY755663; organism=Citrus sinensis; type=EST; length=896bp
AGTAGAACTGCGTGAAGAAGAAAATTGAAAGAGAGGAATCACTGTAGCGT
ATAATCAACAATGAGTAGAGGAAGTGGAGGAGGATACGATCGTCATATTA
CAATTTTTTCGCCTGAGGGTCGTCTCTTTCAAGTCGAGTATGCTTTCAAA
GCTGTTAAGGCTGCTGGGGTTACCTCTATTGGTGTTCGAGGAAAGGATTC
AGTCTGTGTTGTGACTCAGAAGAAGGTCCCGGACAAGCTTTTGGATCATA
CAAGTGTCACACATCTTTTCCCAATCACAAAGTACCTTGGATTGCTGGCA
ACTGGAATGACAGCTGATGCAAGGACATTGGTTCAGCAGGCAAGATATGA
AGCCGCTGAGTTTCGCTTCAAATATGGATATGAAATGCCTGTGGATGTGT
TAGCCAAATGGATTGCAGACAAATCACAAGTATATACTCAACATGCTTAT
ATGAGACCACTTGGTGTAGTTGCTATGGTTTTGAGTATTGACGAAGAATG
TGGACCTCGCCTCTTTAAGTGTGACCCTGCTGGCCATTTCTTTGGCCACA
AGGCAACAAGTGCTGGATTGAAGGAGCAAGAAGCAATTAATTTCTTGGAG
AAGAGATGAAGAATGATCCTGCGTTTACCTTTCAAGAAACTGTGCAGACT
GCAATTTCCACCCTGCAATCAGTCCTGCAAGAAGATTTTAAGGCGAGTGA
NATTGAGGGTTGCGTGGTGAGCAAGGAGATCCANAATCANAGTTTTGTCC
ATCGAAGAGATCGATGAGCACTTGACTGCTTTAAGTGAACGAGATAAGGA
GGTGGTTCATGCCCAGTCCAAATGCTGAAACAAAAACAAAATGCTTTNCC
CTGTATCTGATTATATTTTAGTGTATTCGTAATCCACACCCTTGCC
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