EY729380

Overview
NameEY729380
Unique NameEY729380
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length834
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig8920 contig Csv1_Contig8920:1..834. BLAST: Citrus Unigene V1 Contigs to SwissProt
Homology
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEFS (Ribonuclease PH OS=Pseudomonas fluorescens (strain SBW25) GN=rph PE=3 SV=1)

HSP 1 Score: 239.195 bits (609), Expect = 2.185e-62
Identity = 123/124 (99.19%), Postives = 123/124 (99.19%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLK GDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKAGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEF5 (Ribonuclease PH OS=Pseudomonas fluorescens (strain Pf-5 / ATCC BAA-477) GN=rph PE=3 SV=1)

HSP 1 Score: 233.032 bits (593), Expect = 1.566e-60
Identity = 119/124 (95.97%), Postives = 122/124 (98.39%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPA+LNAMLALAQ+GM EIF+LQ AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPAELNAMLALAQQGMNEIFQLQQAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEU2 (Ribonuclease PH OS=Pseudomonas syringae pv. syringae (strain B728a) GN=rph PE=3 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 4.555e-60
Identity = 118/124 (95.16%), Postives = 120/124 (96.77%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVAL DALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTS GGFIEVQGTAEGAPFQP +LNAMLALAQKGMTE+FELQ AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALADALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSAGGFIEVQGTAEGAPFQPEELNAMLALAQKGMTELFELQRAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSESM (Ribonuclease PH OS=Pseudomonas syringae pv. tomato GN=rph PE=3 SV=1)

HSP 1 Score: 230.72 bits (587), Expect = 7.770e-60
Identity = 117/124 (94.35%), Postives = 120/124 (96.77%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVAL DALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTS GGFIEVQGTAEGAPFQP +LNAMLALAQKGMTE+FE+Q AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALADALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSAGGFIEVQGTAEGAPFQPEELNAMLALAQKGMTELFEMQRAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEPF (Ribonuclease PH OS=Pseudomonas fluorescens (strain Pf0-1) GN=rph PE=3 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 2.953e-59
Identity = 117/124 (94.35%), Postives = 119/124 (95.97%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMI AVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQP +LNAML LA+KGM EIFELQ AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIGAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPEELNAMLELAKKGMNEIFELQKAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEPK (Ribonuclease PH OS=Pseudomonas putida (strain KT2440) GN=rph PE=3 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 8.041e-57
Identity = 114/124 (91.94%), Postives = 115/124 (92.74%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVAL DAL VIKKRGGLKGG+PLK MIAAVSVGMYQGE VLDLDYLEDSAAETDLNVVMTS GGFIEVQGTAEGAPFQP D NAMLALAQKGM EIFELQ AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALCDALAVIKKRGGLKGGNPLKHMIAAVSVGMYQGEAVLDLDYLEDSAAETDLNVVMTSAGGFIEVQGTAEGAPFQPEDFNAMLALAQKGMNEIFELQQAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEP1 (Ribonuclease PH OS=Pseudomonas putida (strain F1 / ATCC 700007) GN=rph PE=3 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 8.041e-57
Identity = 114/124 (91.94%), Postives = 115/124 (92.74%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVAL DAL VIKKRGGLKGG+PLK MIAAVSVGMYQGE VLDLDYLEDSAAETDLNVVMTS GGFIEVQGTAEGAPFQP D NAMLALAQKGM EIFELQ AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALCDALAVIKKRGGLKGGNPLKHMIAAVSVGMYQGEAVLDLDYLEDSAAETDLNVVMTSAGGFIEVQGTAEGAPFQPEDFNAMLALAQKGMNEIFELQQAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEPW (Ribonuclease PH OS=Pseudomonas putida (strain W619) GN=rph PE=3 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 3.055e-56
Identity = 113/124 (91.13%), Postives = 114/124 (91.94%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVALVDAL VIKKRGGLK G+PLK MIAAVSVGMYQGE VLDLDYLEDSAAETDLNVVMTS GGFIEVQGTAEGAPFQP D NAMLALAQKGM EIFELQ  ALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALVDALAVIKKRGGLKAGNPLKHMIAAVSVGMYQGEAVLDLDYLEDSAAETDLNVVMTSAGGFIEVQGTAEGAPFQPEDFNAMLALAQKGMNEIFELQQVALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEPG (Ribonuclease PH OS=Pseudomonas putida (strain GB-1) GN=rph PE=3 SV=1)

HSP 1 Score: 215.698 bits (548), Expect = 2.587e-55
Identity = 112/124 (90.32%), Postives = 113/124 (91.13%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVAL DAL VIKKRGGLK G+PLK MIAAVSVGMYQGE VLDLDY EDSAAETDLNVVMTS GGFIEVQGTAEGAPFQP D NAMLALAQKGM EIFELQ AALAD
Sbjct:  117 CDVIQADGGTRTASITGAMVALCDALAVIKKRGGLKAGNPLKHMIAAVSVGMYQGEAVLDLDYPEDSAAETDLNVVMTSAGGFIEVQGTAEGAPFQPEDFNAMLALAQKGMNEIFELQQAALAD 240
[Close]
BLAST of EY729380 vs. ExPASy Swiss-Prot
Match: RNPH_PSEMY (Ribonuclease PH OS=Pseudomonas mendocina (strain ymp) GN=rph PE=3 SV=1)

HSP 1 Score: 214.157 bits (544), Expect = 7.526e-55
Identity = 110/124 (88.71%), Postives = 116/124 (93.55%), Query Frame = 1 
Query:    1 CDVIQADGGTRTASITGAMVALVDALKVIKKRGGLKGGDPLKQMIAAVSVGMYQGEPVLDLDYLEDSAAETDLNVVMTSTGGFIEVQGTAEGAPFQPADLNAMLALAQKGMTEIFELQNAALAD 372
            CDVIQADGGTRTASITGAMVAL+DALKV+KKRG LKG +PLKQMIAAVSVG+YQGEPVLDLDYLEDSAAETDLNVVMT+ GGFIEVQGTAEGAPFQP + NAMLALAQKGM EIFELQ AAL D
Sbjct:  117 CDVIQADGGTRTASITGAMVALIDALKVLKKRGSLKG-EPLKQMIAAVSVGIYQGEPVLDLDYLEDSAAETDLNVVMTNAGGFIEVQGTAEGAPFQPEEFNAMLALAQKGMNEIFELQKAALVD 239
The following BLAST results are available for this feature:
BLAST of EY729380 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 423
Match NameE-valueIdentityDescription
RNPH_PSEFS2.185e-6299.19Ribonuclease PH OS=Pseudomonas fluorescens (strain... [more]
RNPH_PSEF51.566e-6095.97Ribonuclease PH OS=Pseudomonas fluorescens (strain... [more]
RNPH_PSEU24.555e-6095.16Ribonuclease PH OS=Pseudomonas syringae pv. syring... [more]
RNPH_PSESM7.770e-6094.35Ribonuclease PH OS=Pseudomonas syringae pv. tomato... [more]
RNPH_PSEPF2.953e-5994.35Ribonuclease PH OS=Pseudomonas fluorescens (strain... [more]
RNPH_PSEPK8.041e-5791.94Ribonuclease PH OS=Pseudomonas putida (strain KT24... [more]
RNPH_PSEP18.041e-5791.94Ribonuclease PH OS=Pseudomonas putida (strain F1 /... [more]
RNPH_PSEPW3.055e-5691.13Ribonuclease PH OS=Pseudomonas putida (strain W619... [more]
RNPH_PSEPG2.587e-5590.32Ribonuclease PH OS=Pseudomonas putida (strain GB-1... [more]
RNPH_PSEMY7.526e-5588.71Ribonuclease PH OS=Pseudomonas mendocina (strain y... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-016-B10-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY729380 ID=EY729380; Name=EY729380; organism=Citrus sinensis; type=EST; length=834bp
TGCGACGTGATCCAGGCTGACGGTGGCACTCGCACGGCGTCCATCACCGG
CGCTATGGTTGCCCTGGTCGATGCGCTCAAAGTCATCAAGAAGCGTGGCG
GCCTGAAAGGCGGCGATCCGCTCAAGCAGATGATCGCGGCTGTATCGGTA
GGCATGTATCAGGGCGAGCCAGTGCTGGATCTGGACTATCTTGAAGATTC
GGCTGCCGAGACCGACCTGAACGTGGTGATGACCAGCACCGGTGGTTTCA
TCGAAGTGCAGGGCACCGCCGAAGGCGCGCCATTCCAGCCGGCGGACCTG
AATGCCATGCTGGCATTGGCCCAGAAAGGCATGACCGAGATCTTCGAACT
GCAGAACGCCGCCCTGGCCGACTGATTCAACCCCAAGGAGCTACGCCATG
AGTGACAGTCAACTTCCGCTGCCGACACCGTCCAAGGAAGTTCGCCAGTG
GGCGATGCTCTGTCACTTCGCGGCGTTTTTCGGCTTGATGTTCCCGTTCG
GCAGCCTGCTGGGGCCTTTGATTCTCTGGCAGGTGAAGAAGGATACGGAC
CCGTTCATTGATGATCAGGGCAAGGAAGCGCTGAATTTCCAGATCACCGT
GGGCATTGCCTGGGCGGTCTGCTTCCTGCTGGGTTTTATCGTGATCGGCT
TCGTGTTGATGACCGTCCTGGTGATCGGCGCCGTGGTGATGACGATCATT
GGGGGCATCAAGCCCACAAGGGCGTGGCTTATCGTTATCCGTGGACGTTG
CGGTTGATCAAATAACACCCGCCCACAAAAAACGACAGNGATGTCGGTTT
TTTTGTGCCCGGGAAAAAACCTAAAATGGCATGT
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