EY729212 | Citrus Genome Database

Overview


Name	EY729212
Unique Name	EY729212
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	895

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: DCS4_GOSAR ((+)-delta-cadinene synthase isozyme C2 OS=Gossypium arboreum GN=CAD1-C2 PE=2 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 2.070e-29
Identity = 51/122 (41.80%), Postives = 71/122 (58.20%), Query Frame = 2

Query:   50 SSGETFRPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSN--RTDLHTGSPHFPMRKRPRIPNSCD 409
            S+ +  RP ADF P++W + FL      K +D   T++R++ LKE+VR+MI        QKL  ID +QRLGV YHF KEIED   +L +IY ++N    DL+T S  F + +      SCD
Sbjct:   16 SNKDEIRPKADFQPSIWGDFFLNCPD--KNID-AGTEKRHQQLKEEVRKMIVAPMANSTQKLAFIDSVQRLGVSYHFTKEIED---ELENIYHNNNDAENDLYTTSLRFRLLREHGYNVSCD 131

HSP 2 Score: 50.8322 bits (120), Expect = 2.070e-29
Identity = 27/75 (36.00%), Postives = 49/75 (65.33%), Query Frame = 1

Query:  436 RGVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAH-DHVTPMLAEQINHAVYRPLS*TLPK 657
            +G ++SS+ +D+  +L L +A+Y+ V GE IL + ++FTT HL   V+  DH    L+E+++HA+ + +   LP+
Sbjct:  140 QGNFKSSVTSDVQGLLELYQASYLRVHGEDILDEAISFTTNHLSLAVSSLDH---PLSEEVSHALKQSIRRGLPR 211

HSP 3 Score: 37.3502 bits (85), Expect = 2.070e-29
Identity = 20/50 (40.00%), Postives = 28/50 (56.00%), Query Frame = 2

Query:  665 EVVYVLDQFHSDHLYNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEF 811
            E  + L  +     +N  LL FAK+DFN+L  L R   S I  WW++L+F
Sbjct:  213 EARHYLSVYQDIESHNKALLEFAKIDFNMLQFLHRKELSEICRWWKDLDF 262

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: DCS1_GOSAR ((+)-delta-cadinene synthase isozyme XC1 OS=Gossypium arboreum PE=1 SV=1)

HSP 1 Score: 81.6481 bits (200), Expect = 3.489e-29
Identity = 51/122 (41.80%), Postives = 71/122 (58.20%), Query Frame = 2

Query:   50 SSGETFRPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSN--RTDLHTGSPHFPMRKRPRIPNSCD 409
            S+ +  RP ADF P++W + FL      K +D   T++R++ LKE+VR+MI        QKL  ID +QRLGV YHF KEIED   +L +IY ++N    DL+T S  F + +      SCD
Sbjct:   16 SNKDEMRPKADFQPSIWGDLFLNCPD--KNID-AETEKRHQQLKEEVRKMIVAPMANSTQKLAFIDSVQRLGVSYHFTKEIED---ELENIYHNNNDAENDLYTTSIRFRLLREHGYNVSCD 131

HSP 2 Score: 51.2174 bits (121), Expect = 3.489e-29
Identity = 28/75 (37.33%), Postives = 49/75 (65.33%), Query Frame = 1

Query:  436 RGVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAH-DHVTPMLAEQINHAVYRPLS*TLPK 657
            +G ++SS+ +D+  +L L +A+Y+ V GE IL + ++FTT HL   VA  DH    L+E+++HA+ + +   LP+
Sbjct:  140 QGNFKSSVTSDVRGLLELYQASYLRVHGEDILDEAISFTTHHLSLAVASLDH---PLSEEVSHALKQSIRRGLPR 211

HSP 3 Score: 37.3502 bits (85), Expect = 3.489e-29
Identity = 20/50 (40.00%), Postives = 28/50 (56.00%), Query Frame = 2

Query:  665 EVVYVLDQFHSDHLYNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEF 811
            E  + L  +     +N  LL FAK+DFN+L  L R   S I  WW++L+F
Sbjct:  213 EARHYLSVYQDIESHNKALLEFAKIDFNMLQFLHRKELSEICRWWKDLDF 262

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: DCS2_GOSAR ((+)-delta-cadinene synthase isozyme XC14 OS=Gossypium arboreum PE=2 SV=1)

HSP 1 Score: 82.0333 bits (201), Expect = 4.530e-29
Identity = 51/122 (41.80%), Postives = 71/122 (58.20%), Query Frame = 2

Query:   50 SSGETFRPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSN--RTDLHTGSPHFPMRKRPRIPNSCD 409
            S+ +  RP ADF P++W + FL      K +D   T++R++ LKE+VR+MI        QKL  ID +QRLGV YHF KEIED   +L +IY ++N    DL+T S  F + +      SCD
Sbjct:   16 SNKDEMRPKADFQPSIWGDLFLNCPD--KNID-AETEKRHQQLKEEVRKMIVAPMANSTQKLAFIDSVQRLGVSYHFTKEIED---ELENIYHNNNDAENDLYTTSLRFRLLREHGFNVSCD 131

HSP 2 Score: 50.447 bits (119), Expect = 4.530e-29
Identity = 26/74 (35.14%), Postives = 48/74 (64.86%), Query Frame = 1

Query:  436 RGVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAHDHVTPMLAEQINHAVYRPLS*TLPK 657
            +G ++SS+ +D+  +L L +A+Y+ V GE IL + ++FTT HL   VA   +   L+E+++HA+ + +   LP+
Sbjct:  140 QGNFKSSVTSDVRGLLELYQASYLRVHGEDILDEAISFTTNHLSLAVA--SLDYPLSEEVSHALKQSIRRGLPR 211

HSP 3 Score: 37.3502 bits (85), Expect = 4.530e-29
Identity = 19/50 (38.00%), Postives = 29/50 (58.00%), Query Frame = 2

Query:  665 EVVYVLDQFHSDHLYNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEF 811
            E  + L  +     +N  LL FAK+DFN++  L R   S I+ WW++L+F
Sbjct:  213 EARHYLSVYQDIESHNKVLLEFAKIDFNMVQLLHRKELSEISRWWKDLDF 262

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: DCS3_GOSAR ((+)-delta-cadinene synthase isozyme A OS=Gossypium arboreum GN=CAD1-A PE=2 SV=1)

HSP 1 Score: 81.2629 bits (199), Expect = 2.169e-28
Identity = 51/119 (42.86%), Postives = 69/119 (57.98%), Query Frame = 2

Query:   68 RPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSNR----TDLHTGSPHFPMRKRPRIPNSCDS 412
            RP ADFHP +W + F+       T    AT+ + E LK +VR+MI +  +   QKL  ID +QRLGV YHFEKEIED   +L +IY D+N     TDL+T +  F + +      SCD+
Sbjct:   21 RPKADFHPGIWGDMFIICPD---TDIDAATELQYEELKAQVRKMIMEPVDDSNQKLPFIDAVQRLGVSYHFEKEIED---ELENIYRDTNNNDADTDLYTTALRFRLLREHGFDISCDA 133

HSP 2 Score: 48.1358 bits (113), Expect = 2.169e-28
Identity = 27/73 (36.99%), Postives = 44/73 (60.27%), Query Frame = 1

Query:  439 GVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAHDHVTPMLAEQINHAVYRPLS*TLPK 657
            G +++SL +D+  +L L EA+YM V GE IL + ++FTT  L   +   H    L+EQ+ HA+ + +   LP+
Sbjct:  142 GNFKASLTSDVQGLLELYEASYMRVHGEDILDEAISFTTAQLTLALPTLH--HPLSEQVGHALKQSIRRGLPR 212

HSP 3 Score: 38.1206 bits (87), Expect = 2.169e-28
Identity = 18/37 (48.65%), Postives = 26/37 (70.27%), Query Frame = 2

Query:  707 YNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEFS 814
            +N +LL FAK+DFN+L  L R   S I  WW++L+F+
Sbjct:  228 HNKSLLQFAKIDFNLLQLLHRKELSEICRWWKDLDFT 264

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: DCS1_GOSHI ((+)-delta-cadinene synthase OS=Gossypium hirsutum GN=CDN1 PE=1 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 4.745e-28
Identity = 50/122 (40.98%), Postives = 69/122 (56.56%), Query Frame = 2

Query:   50 SSGETFRPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSN--RTDLHTGSPHFPMRKRPRIPNSCD 409
            S+ +  RP ADF P++W + FL      K +D   TQ+R++ LKE+VR+MI         KL  ID +Q LGV YHF KEIED   +L +IY ++N    DL+T S  F + +      SCD
Sbjct:   16 SNKDEMRPKADFQPSIWGDFFLNCPD--KNID-AETQKRHQQLKEEVRKMIVAPMANSTLKLAFIDSVQGLGVSYHFTKEIED---ELENIYHNNNDAENDLYTTSLRFRLLREHGFHVSCD 131

HSP 2 Score: 50.447 bits (119), Expect = 4.745e-28
Identity = 27/75 (36.00%), Postives = 49/75 (65.33%), Query Frame = 1

Query:  436 RGVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAH-DHVTPMLAEQINHAVYRPLS*TLPK 657
            +G ++SS+ +D+  +L L +A+Y+ V GE IL + ++FT+ HL   VA  DH    L+E+++HA+ + +   LP+
Sbjct:  140 QGNFKSSVTSDVRGLLELYQASYLRVHGEDILDEAISFTSNHLSLAVASLDH---PLSEEVSHALKQSIRRGLPR 211

HSP 3 Score: 37.3502 bits (85), Expect = 4.745e-28
Identity = 19/50 (38.00%), Postives = 29/50 (58.00%), Query Frame = 2

Query:  665 EVVYVLDQFHSDHLYNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEF 811
            E  + L  +     +N  LL FAK+DFN++  L R   S I+ WW++L+F
Sbjct:  213 EARHYLSVYQDIESHNKVLLEFAKIDFNMVQLLHRKELSEISRWWKDLDF 262

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: 5EAS_TOBAC (Aristolochene synthase OS=Nicotiana tabacum GN=EAS3 PE=1 SV=3)

HSP 1 Score: 71.2478 bits (173), Expect = 6.727e-26
Identity = 42/109 (38.53%), Postives = 62/109 (56.88%), Query Frame = 2

Query:   59 ETFRPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSNRTDLHTGSPHFPMRKR 385
            E  RP ADF P+LW + FL  +   +  +  A +   EALKE+ R M+     K    L LID I+RLG+ YHFEKEI+D + ++ +   +SN  DL T +  F + ++
Sbjct:   12 EIVRPVADFSPSLWGDQFLSFSIKNQVAEKYAKEI--EALKEQTRNMLLATGMKLADTLNLIDTIERLGISYHFEKEIDDILDQIYN--QNSNCNDLCTSALQFRLLRQ 116

HSP 2 Score: 48.9062 bits (115), Expect = 6.727e-26
Identity = 27/73 (36.99%), Postives = 45/73 (61.64%), Query Frame = 1

Query:  439 GVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAHDHVTPMLAEQINHAVYRPLS*TLPK 657
            G ++ SL +D+  +LNL EA+++    + IL D +AF+T HL+S  A  H+   L EQ+ HA+ + L   +P+
Sbjct:  134 GKFKESLASDVLGLLNLYEASHVRTHADDILEDALAFSTIHLES--AAPHLKSPLREQVTHALEQCLHKGVPR 204

HSP 3 Score: 38.891 bits (89), Expect = 6.727e-26
Identity = 17/47 (36.17%), Postives = 29/47 (61.70%), Query Frame = 2

Query:  674 YVLDQFHSDHLYNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEF 811
            ++   +  +   NN LL FAKLDFN+L  L +   + ++ WW++L+F
Sbjct:  210 FISSIYDKEQSKNNVLLRFAKLDFNLLQMLHKQELAQVSRWWKDLDF 256

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: ECS1_ARTAN (Epi-cedrol synthase OS=Artemisia annua GN=ECS1 PE=1 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 8.849e-18
Identity = 30/94 (31.91%), Postives = 48/94 (51.06%), Query Frame = 2

Query:   59 ETFRPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQK---LRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYID 331
            +  RP A+F   +W + FL    D    +    ++  + LKE+V+  +  A N P Q    L+ ID I+RLG+ Y+FE+EI      + + Y D
Sbjct:    7 DVIRPNANFPSEIWGDQFLAYDQD----EQEGVEQVIKDLKEEVKSELLTALNSPTQHTELLKFIDAIERLGIAYYFEEEINQVFQHMYTAYGD 96

HSP 2 Score: 48.9062 bits (115), Expect = 8.849e-18
Identity = 29/74 (39.19%), Postives = 43/74 (58.11%), Query Frame = 1

Query:  439 GVYQSSLINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAHDHVT-PMLAEQINHAVYRPLS*TLPK 657
            G ++ SL  D+H +L L EAAYM V GE IL D + FT T L  +  +  ++   ++ QI  A+ +PL   LP+
Sbjct:  130 GRFKESLEKDVHGLLELYEAAYMFVPGEGILDDALVFTRTCLDEIAKNPSLSNSAVSSQIREALTQPLHKRLPR 203

HSP 3 Score: 29.261 bits (64), Expect = 8.849e-18
Identity = 17/49 (34.69%), Postives = 28/49 (57.14%), Query Frame = 2

Query:  707 YNNNLLXFAKLDFNILLELPRTN-SMINNWWEELEFSSGHLIQKRNKDV 850
            ++  LL  AKL FN L  L +   S+I+ WW+  + ++ +L   RN+ V
Sbjct:  219 HSETLLKLAKLGFNQLQSLHKKELSIISKWWKSFDVAN-NLPYARNRPV 266

[Close]

BLAST of EY729212 vs. ExPASy Swiss-Prot
Match: RLC1_CITLI ((R)-limonene synthase 1 OS=Citrus limon PE=2 SV=1)

HSP 1 Score: 51.6026 bits (122), Expect = 9.291e-11
Identity = 35/114 (30.70%), Postives = 63/114 (55.26%), Query Frame = 2

Query:   68 RPTADFHPNLWRNHFLKGASDFKTVDHTATQERNEALKEKVREMITDAENKPVQKLRLIDEIQRLGVGYHFEKEIEDAIPKLCSIYIDSN--RTDLHTGSPHFPMRKRPRIPNS 403
            R +A++ P++W + FL+  +   T +  A + R E L+ KV+  I D   +P+ +L LID +QRLG+ + FE EI + +  + +   D N  + +L+  S  F + ++   P S
Sbjct:   53 RRSANYQPSIWDHDFLQSLNSNYTDE--AYKRRAEELRGKVKIAIKDVI-EPLDQLELIDNLQRLGLAHRFETEIRNILNNIYNNNKDYNWRKENLYATSLEFRLLRQHGYPVS 163

HSP 2 Score: 36.1946 bits (82), Expect = 9.291e-11
Identity = 21/68 (30.88%), Postives = 38/68 (55.88%), Query Frame = 1

Query:  457 LINDIHRMLNLCEAAYMAVRGEHILYDDVAFTTTHLKSLVAHDHVTP--MLAEQINHAVYRPLS*TLP 654
            + +D   +L+L EA+Y ++ GE I+ +   FT+ HLK ++   ++     +AEQ   A+  PL   +P
Sbjct:  178 ICDDFKGILSLHEASYYSLEGESIMEEAWQFTSKHLKEVMISKNMEEDVFVAEQAKRALELPLHWKVP 245

The following BLAST results are available for this feature:

BLAST of EY729212 vs. ExPASy Swiss-Prot

BLAST of EY729212 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 8


Match Name	E-value	Identity	Description
DCS4_GOSAR	2.070e-29	41.80	(+)-delta-cadinene synthase isozyme C2 OS=Gossypiu...	[more]
DCS1_GOSAR	3.489e-29	41.80	(+)-delta-cadinene synthase isozyme XC1 OS=Gossypi...	[more]
DCS2_GOSAR	4.530e-29	41.80	(+)-delta-cadinene synthase isozyme XC14 OS=Gossyp...	[more]
DCS3_GOSAR	2.169e-28	42.86	(+)-delta-cadinene synthase isozyme A OS=Gossypium...	[more]
DCS1_GOSHI	4.745e-28	40.98	(+)-delta-cadinene synthase OS=Gossypium hirsutum ...	[more]
5EAS_TOBAC	6.727e-26	38.53	Aristolochene synthase OS=Nicotiana tabacum GN=EAS...	[more]
ECS1_ARTAN	8.849e-18	31.91	Epi-cedrol synthase OS=Artemisia annua GN=ECS1 PE=...	[more]
RLC1_CITLI	9.291e-11	30.70	(R)-limonene synthase 1 OS=Citrus limon PE=2 SV=1	[more]

Properties


Property Name	Value
Genbank description	CS00-C3-704-014-A01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY729212 ID=EY729212; Name=EY729212; organism=Citrus sinensis; type=EST; length=895bp
CCACGCGTCCGCAAAATTCTGCAGCACCCGTTTGTCTGACACAAAAATGT
CGTCTGGAGAAACATTTCGTCCTACTGCAGATTTCCATCCTAATTTATGG
AGAAACCATTTCCTCAAAGGTGCTTCTGATTTCAAGACAGTTGATCATAC
TGCAACTCAAGAACGAAACGAAGCACTGAAAGAAAAAGTTAGGGAAATGA
TAACAGATGCTGAAAATAAACCTGTTCAAAAATTACGCTTGATTGATGAA
ATACAACGCCTGGGGGTGGGTTATCACTTTGAGAAAGAAATTGAAGATGC
CATACCAAAATTATGTTCCATCTATATTGACAGTAATAGAACTGATCTCC
ACACCGGTTCCCCTCATTTTCCAATGCGTAAGCGGCCAAGGATACCAAAT
TCATGTGATAGTGTGTGAGAAATTCTCAAATGATGAGAGGTGTATACCAG
TCATCGTTGATAAACGATATTCACAGGATGTTAAATTTGTGCGAGGCGGC
ATATATGGCAGTTCGCGGAGAACATATATTATATGATGACGTTGCTTTCA
CTACCACTCACCTGAAATCATTGGTAGCTCACGATCATGTAACCCCTATG
CTTGCGGAGCAGATAAATCATGCTGTATACCGTCCTCTCTCGTAAACCCT
ACCAAAATATATGCGAGGTAGTTTATGTCCTTGATCAATTTCACAGTGAT
CATTTATACAATAACAATCTGCTGANTTTTGCAAAGTTAGATTTTAACAT
ATTGCTAGAACTGCCCCGGACGAACTCAATGATTAACAATTGGTGGGAGG
AATTAGAATTCTCTTCTGGACACCTTATCCAAAAACGAAATAAAGATGTT
ATTTTGGGATTGAGGGGCGTGTTCCAGCCTCATTTGGTTGGGGAG