EY728517 | Citrus Genome Database

Overview


Name	EY728517
Unique Name	EY728517
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	902

Libraries


Library Name	Type
Sweet orange fruit, development stadium (5 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1B_ARATH (GTP-binding protein SAR1B OS=Arabidopsis thaliana GN=SAR1B PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 1.713e-66
Identity = 111/128 (86.72%), Postives = 112/128 (87.50%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGH IARRVWKDYYAKVDAVVYLVD+YDKERFAESKR             VPFL  G
Sbjct:    1 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKRELDALLSDEALATVPFLILG 128

HSP 2 Score: 49.6766 bits (117), Expect = 1.713e-66
Identity = 24/36 (66.67%), Postives = 29/36 (80.56%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGLTSFSTGQW*VNRVDS 611
            G++IDIPYAASEDEL Y LGLT+F+TG+  V   DS
Sbjct:  128 GNKIDIPYAASEDELRYHLGLTNFTTGKGKVTLGDS 163

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1A_ARATH (GTP-binding protein SAR1A OS=Arabidopsis thaliana GN=SAR1A PE=2 SV=1)

HSP 1 Score: 220.705 bits (561), Expect = 6.460e-66
Identity = 107/128 (83.59%), Postives = 111/128 (86.72%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            MF+ DWFYG+LASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGH IARRVWKDYYAKVDAVVYLVD+YDKERFAESK+             VPFL  G
Sbjct:    1 MFMIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKKELDALLSDESLASVPFLILG 128

HSP 2 Score: 52.373 bits (124), Expect = 6.460e-66
Identity = 24/38 (63.16%), Postives = 32/38 (84.21%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGLTSFSTGQW*VNRVDSHL 617
            G++IDIPYAASEDEL Y LGL++F+TG+  VN  DS++
Sbjct:  128 GNKIDIPYAASEDELRYHLGLSNFTTGKGKVNLTDSNV 165

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1A_BRACM (GTP-binding protein SAR1A OS=Brassica campestris GN=SAR1A PE=2 SV=1)

HSP 1 Score: 220.32 bits (560), Expect = 7.043e-65
Identity = 107/128 (83.59%), Postives = 111/128 (86.72%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            MF+ DWFYG+LASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGH IARRVWKDYYAKVDAVVYLVD+YDKERFAESK+             VPFL  G
Sbjct:    1 MFVIDWFYGVLASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFAESKKELDALLSDESLATVPFLILG 128

HSP 2 Score: 49.2914 bits (116), Expect = 7.043e-65
Identity = 23/38 (60.53%), Postives = 32/38 (84.21%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGLTSFSTGQW*VNRVDSHL 617
            G++IDIPYAASEDEL Y LGL++F+TG+  V+ V S++
Sbjct:  128 GNKIDIPYAASEDELRYHLGLSNFTTGKGKVDLVGSNV 165

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1B_BRACM (GTP-binding protein SAR1B OS=Brassica campestris GN=SAR1B PE=2 SV=1)

HSP 1 Score: 223.016 bits (567), Expect = 1.197e-64
Identity = 109/128 (85.16%), Postives = 112/128 (87.50%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGH IARRVWKDYYAKVDAVVYLVD+YDKERF+ESK+             VPFL  G
Sbjct:    1 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDAYDKERFSESKKELDALLSDDALATVPFLILG 128

HSP 2 Score: 45.8246 bits (107), Expect = 1.197e-64
Identity = 20/28 (71.43%), Postives = 25/28 (89.29%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGLTSFSTGQ 587
            G++ID PYAASEDEL Y LGLT+F+TG+
Sbjct:  128 GNKIDNPYAASEDELRYHLGLTNFTTGK 155

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR2_SOLLC (GTP-binding protein SAR2 OS=Solanum lycopersicum GN=SAR2 PE=2 SV=1)

HSP 1 Score: 206.838 bits (525), Expect = 4.878e-60
Identity = 100/128 (78.12%), Postives = 108/128 (84.38%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            MFL DWFYG+LASLGLWQK+AKILFLGLDNAGKTTLLHMLKDERLVQHQPTQ+PTSEELSIG IKFKAFDLGGH IARRVW+DYYAKVDAVVYLVD+ D+ERF E+K+             VPFL  G
Sbjct:    1 MFLVDWFYGVLASLGLWQKDAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGNIKFKAFDLGGHQIARRVWRDYYAKVDAVVYLVDANDRERFPEAKKELDGLLSDESLTNVPFLILG 128

HSP 2 Score: 46.595 bits (109), Expect = 4.878e-60
Identity = 21/32 (65.62%), Postives = 26/32 (81.25%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGLTSFSTGQW*VN 599
            G++IDIPYAASEDEL Y LGLT  +TG+  +N
Sbjct:  128 GNKIDIPYAASEDELRYHLGLTGVTTGKGNIN 159

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1_TOBAC (GTP-binding protein SAR1 OS=Nicotiana tabacum GN=SAR1 PE=2 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 1.885e-54
Identity = 102/108 (94.44%), Postives = 106/108 (98.15%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKR 444
            MFL DWFYGILA+LGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQ+PTSEELSIGKIKFKAFDLGGH IARRVWKDYYAKVDAVVYLVDS+DKERFAESK+
Sbjct:    1 MFLVDWFYGILATLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQYPTSEELSIGKIKFKAFDLGGHQIARRVWKDYYAKVDAVVYLVDSFDKERFAESKK 108

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1_PICST (Small COPII coat GTPase SAR1 OS=Pichia stipitis GN=SAR1 PE=3 SV=1)

HSP 1 Score: 166.777 bits (421), Expect = 1.661e-41
Identity = 79/128 (61.72%), Postives = 95/128 (74.22%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            M+LFDWF  +L+SLGLW K AK+LFLGLDNAGKTTLLHMLK++RL   QPT HPTSEEL+IG ++F  FDLGGH  ARR+WKDY+ +V+ +V+LVD+ D ERFAESK              VPFL  G
Sbjct:    1 MWLFDWFQDVLSSLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGSVRFTTFDLGGHQQARRLWKDYFPEVNGIVFLVDAADPERFAESKAELESLFKIEELSHVPFLILG 128

HSP 2 Score: 24.6386 bits (52), Expect = 1.661e-41
Identity = 11/21 (52.38%), Postives = 14/21 (66.67%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGL 566
            G++ID+P A  E EL   LGL
Sbjct:  128 GNKIDVPTAVGEMELKSALGL 148

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1_LODEL (Small COPII coat GTPase SAR1 OS=Lodderomyces elongisporus GN=SAR1 PE=3 SV=2)

HSP 1 Score: 166.777 bits (421), Expect = 1.661e-41
Identity = 79/128 (61.72%), Postives = 95/128 (74.22%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            M++FDWF  ILASLGLW K AK+LFLGLDNAGKTTLLHMLK++RL   QPT HPTSEEL+IG ++F  FDLGGH  ARR+WKDY+ +V+ +V+LVD+ D ERFAESK              VPF+  G
Sbjct:    1 MWIFDWFQDILASLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGSVRFTTFDLGGHQQARRLWKDYFPEVNGIVFLVDAADSERFAESKAELESLFKIEELSQVPFVILG 128

HSP 2 Score: 24.6386 bits (52), Expect = 1.661e-41
Identity = 11/21 (52.38%), Postives = 14/21 (66.67%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGL 566
            G++ID+P A  E EL   LGL
Sbjct:  128 GNKIDVPTAVGEMELKNALGL 148

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1_CANAL (Small COPII coat GTPase SAR1 OS=Candida albicans GN=SAR1 PE=3 SV=2)

HSP 1 Score: 165.236 bits (417), Expect = 4.788e-41
Identity = 78/128 (60.94%), Postives = 95/128 (74.22%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            M++FDWF  IL+SLGLW K AK+LFLGLDNAGKTTLLHMLK++RL   QPT HPTSEEL+IG ++F  FDLGGH  ARR+WKDY+ +V+ +V+LVD+ D ERFAESK              VPF+  G
Sbjct:    1 MWIFDWFQDILSSLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGSVRFTTFDLGGHQQARRLWKDYFPEVNGIVFLVDAADTERFAESKAELESLFRIEELSQVPFVILG 128

HSP 2 Score: 24.6386 bits (52), Expect = 4.788e-41
Identity = 11/21 (52.38%), Postives = 14/21 (66.67%), Query Frame = 3

Query:  504 GSRIDIPYAASEDELAYRLGL 566
            G++ID+P A  E EL   LGL
Sbjct:  128 GNKIDVPTAVGEMELKNALGL 148

[Close]

BLAST of EY728517 vs. ExPASy Swiss-Prot
Match: SAR1_DEBHA (Small COPII coat GTPase SAR1 OS=Debaryomyces hansenii GN=SAR1 PE=3 SV=1)

HSP 1 Score: 167.933 bits (424), Expect = 6.952e-41
Identity = 80/128 (62.50%), Postives = 95/128 (74.22%), Query Frame = 1

Query:  121 MFLFDWFYGILASLGLWQKEAKILFLGLDNAGKTTLLHMLKDERLVQHQPTQHPTSEELSIGKIKFKAFDLGGHHIARRVWKDYYAKVDAVVYLVDSYDKERFAESKRXXXXXXXXXXXXXVPFLSFG 504
            M+LFDWF  +LASLGLW K AK+LFLGLDNAGKTTLLHMLK++RL   QPT HPTSEEL+IG ++F  FDLGGH  ARR+WKDY+ +V+ +V+LVD+ D ERFAESK              VPFL  G
Sbjct:    1 MWLFDWFQDVLASLGLWNKHAKLLFLGLDNAGKTTLLHMLKNDRLATLQPTLHPTSEELAIGSVRFTTFDLGGHQQARRLWKDYFPEVNGIVFLVDAADPERFAESKAELESLFKIEELASVPFLILG 128

The following BLAST results are available for this feature:

BLAST of EY728517 vs. ExPASy Swiss-Prot

BLAST of EY728517 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 155


Match Name	E-value	Identity	Description
SAR1B_ARATH	1.713e-66	86.72	GTP-binding protein SAR1B OS=Arabidopsis thaliana ...	[more]
SAR1A_ARATH	6.460e-66	83.59	GTP-binding protein SAR1A OS=Arabidopsis thaliana ...	[more]
SAR1A_BRACM	7.043e-65	83.59	GTP-binding protein SAR1A OS=Brassica campestris G...	[more]
SAR1B_BRACM	1.197e-64	85.16	GTP-binding protein SAR1B OS=Brassica campestris G...	[more]
SAR2_SOLLC	4.878e-60	78.13	GTP-binding protein SAR2 OS=Solanum lycopersicum G...	[more]
SAR1_TOBAC	1.885e-54	94.44	GTP-binding protein SAR1 OS=Nicotiana tabacum GN=S...	[more]
SAR1_PICST	1.661e-41	61.72	Small COPII coat GTPase SAR1 OS=Pichia stipitis GN...	[more]
SAR1_LODEL	1.661e-41	61.72	Small COPII coat GTPase SAR1 OS=Lodderomyces elong...	[more]
SAR1_CANAL	4.788e-41	60.94	Small COPII coat GTPase SAR1 OS=Candida albicans G...	[more]
SAR1_DEBHA	6.952e-41	62.50	Small COPII coat GTPase SAR1 OS=Debaryomyces hanse...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-704-005-A02-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY728517 ID=EY728517; Name=EY728517; organism=Citrus sinensis; type=EST; length=902bp
CACGCGTCCGATCACGCACTCGTTGATTCCTCCTAATTATCTCAAAGATT
CATCATATTTGAGCCAAAATTCGATCTTTTTGATAGATTCATTATAAATA
AGGAGTAATAAAAGAAACCCATGTTTTTGTTTGATTGGTTTTATGGGATT
CTTGCATCTCTTGGGCTCTGGCAAAAAGAGGCCAAGATCTTGTTTCTCGG
CCTCGATAATGCCGGTAAAACGACGCTGCTTCATATGTTGAAAGATGAGA
GATTAGTTCAACATCAACCAACACAACATCCCACTTCGGAGGAACTGAGT
ATTGGGAAAATCAAGTTTAAGGCTTTTGATTTGGGAGGGCATCACATTGC
TCGTAGAGTTTGGAAAGATTACTACGCCAAGGTTGATGCGGTTGTGTACC
TGGTAGATTCGTATGACAAGGAGAGGTTTGCTGAGTCGAAAAGGGAGCTT
GATGCCCTGCTTGCAGATGAATCCTTGGCCAGTGTACCATTCCTTAGTTT
TGGGGCAGCAGGATTGATATCCCATATGCTGCATCAGAAGATGAGTTGGC
ATATCGCCTTGGCCTCACCAGCTTCAGCACTGGGCAGTGGTAGGTGAATC
GGGTCGATTCGCATCTTCATGCGCCTTGGAGGGGACCGTATACAGGATTG
TACTGAGGAATTGGATACGACAGGGATTGAGGTGTTTGGTCGGGTATTTC
ATTTGGGGGGGGACTAACGGGGGAGGATGTGTGTGGATAGGTGGGGGCGC
GTAGCTGGCGGGGGAGGCGGAGGCCGCGCGCGGGGAGGGTGGGTGAGTGC
CGTCGAGTTGCGAAGTTTTATGGAGGATGTATCGGTTATGGTAGGATTCG
CATGCTGAAATTGTGTGTGTGGGAGAGGGGTGGGCGGGGGGGGCCGTGGA
GG