EY728351

Overview
NameEY728351
Unique NameEY728351
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length811
Libraries
Library NameType
Sweet orange fruit, development stadium (5 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_BACFR (ATP-dependent Clp protease ATP-binding subunit clpX OS=Bacteroides fragilis GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.820e-16
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            G+NP   R VSGEG+  GLLK+L+ + +NVPP   G RK P  + IP+ TK++ F+
Sbjct:  194 GDNPSITRDVSGEGVQQGLLKLLEGSVVNVPP--QGGRKHPDQKMIPVNTKNILFI 247

HSP 2 Score: 52.373 bits (124), Expect = 1.820e-16
Identity = 24/35 (68.57%), Postives = 29/35 (82.86%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHV 117
            PFTI DAT LT+AGYVGED ES+L +LL  AD++V
Sbjct:  140 PFTIVDATVLTEAGYVGEDIESILTRLLQVADYNV 174
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_BACFN (ATP-dependent Clp protease ATP-binding subunit clpX OS=Bacteroides fragilis (strain ATCC 25285 / NCTC 9343) GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.820e-16
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            G+NP   R VSGEG+  GLLK+L+ + +NVPP   G RK P  + IP+ TK++ F+
Sbjct:  194 GDNPSITRDVSGEGVQQGLLKLLEGSVVNVPP--QGGRKHPDQKMIPVNTKNILFI 247

HSP 2 Score: 52.373 bits (124), Expect = 1.820e-16
Identity = 24/35 (68.57%), Postives = 29/35 (82.86%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHV 117
            PFTI DAT LT+AGYVGED ES+L +LL  AD++V
Sbjct:  140 PFTIVDATVLTEAGYVGEDIESILTRLLQVADYNV 174
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_BACTN (ATP-dependent Clp protease ATP-binding subunit clpX OS=Bacteroides thetaiotaomicron GN=clpX PE=3 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.821e-16
Identity = 27/56 (48.21%), Postives = 38/56 (67.86%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            G+NP   R VSGEG+  GLLK+L+ + +NVPP   G RK P  + IP+ TK++ F+
Sbjct:  193 GDNPSITRDVSGEGVQQGLLKLLEGSVVNVPP--QGGRKHPDQKMIPVNTKNILFI 246

HSP 2 Score: 52.373 bits (124), Expect = 1.821e-16
Identity = 24/35 (68.57%), Postives = 29/35 (82.86%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHV 117
            PFTI DAT LT+AGYVGED ES+L +LL  AD++V
Sbjct:  139 PFTIVDATVLTEAGYVGEDIESILTRLLQVADYNV 173
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRSV (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus sanguinis (strain SK36) GN=clpX PE=3 SV=1)

HSP 1 Score: 57.3806 bits (137), Expect = 1.927e-15
Identity = 26/40 (65.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DATALT+AGYVGED E++L +LL AADF++E  +R
Sbjct:  138 PFAIADATALTEAGYVGEDVENILLKLLQAADFNIERAER 177

HSP 2 Score: 46.2098 bits (108), Expect = 1.927e-15
Identity = 25/56 (44.64%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I ++TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPQQEMIQVDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_PORGI (ATP-dependent Clp protease ATP-binding subunit clpX OS=Porphyromonas gingivalis GN=clpX PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.927e-15
Identity = 24/40 (60.00%), Postives = 31/40 (77.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED ES+L +LL AAD+ V+  +R
Sbjct:  138 PFAVVDATVLTEAGYVGEDIESILTRLLQAADYDVKQAER 177

HSP 2 Score: 50.447 bits (119), Expect = 1.927e-15
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+  GLLK+L+ + +NVPP   G RK P  + IP++T+ + F+
Sbjct:  193 DNPSITRDVSGEGVQQGLLKLLEGSIVNVPP--QGGRKHPEQKMIPVDTRHILFV 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_PORG3 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / JCM 12257) GN=clpX PE=3 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.927e-15
Identity = 24/40 (60.00%), Postives = 31/40 (77.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF + DAT LT+AGYVGED ES+L +LL AAD+ V+  +R
Sbjct:  138 PFAVVDATVLTEAGYVGEDIESILTRLLQAADYDVKQAER 177

HSP 2 Score: 50.447 bits (119), Expect = 1.927e-15
Identity = 25/55 (45.45%), Postives = 37/55 (67.27%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            +NP   R VSGEG+  GLLK+L+ + +NVPP   G RK P  + IP++T+ + F+
Sbjct:  193 DNPSITRDVSGEGVQQGLLKLLEGSIVNVPP--QGGRKHPEQKMIPVDTRHILFV 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_CORK4 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Corynebacterium kroppenstedtii (strain DSM 44385 / CCUG 35717) GN=clpX PE=3 SV=1)

HSP 1 Score: 56.6102 bits (135), Expect = 2.502e-15
Identity = 26/40 (65.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF +TDAT+LT+AGYVGED E++L +LL AADF V+  QR
Sbjct:  142 PFAMTDATSLTEAGYVGEDVENILLKLLQAADFDVDRAQR 181

HSP 2 Score: 46.595 bits (109), Expect = 2.502e-15
Identity = 26/55 (47.27%), Postives = 35/55 (63.64%), Query Frame = 3 
Query:  177 ENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            ENP   R VSGEG+   LLK+L+ T  +VPP   G RK P  E I ++TK++ F+
Sbjct:  197 ENPSITRDVSGEGVQQALLKILEGTVASVPP--QGGRKHPNQEFIQLDTKNVLFI 249
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRMU (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus mutans GN=clpX PE=3 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 4.229e-15
Identity = 25/40 (62.50%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DATALT+AGYVGED E++L +L+ AAD++VE  +R
Sbjct:  138 PFAIADATALTEAGYVGEDVENILLKLIQAADYNVERAER 177

HSP 2 Score: 46.595 bits (109), Expect = 4.229e-15
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  192 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPQQEMIQIDTKNILFI 245
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRTD (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus thermophilus (strain ATCC BAA-491 / LMD-9) GN=clpX PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 7.142e-15
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AADF++E  +R
Sbjct:  137 PFAIADATSLTEAGYVGEDVENILLKLIQAADFNIERAER 176

HSP 2 Score: 46.2098 bits (108), Expect = 7.142e-15
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  191 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 244
[Close]
BLAST of EY728351 vs. ExPASy Swiss-Prot
Match: CLPX_STRT2 (ATP-dependent Clp protease ATP-binding subunit clpX OS=Streptococcus thermophilus (strain ATCC BAA-250 / LMG 18311) GN=clpX PE=3 SV=1)

HSP 1 Score: 55.4546 bits (132), Expect = 7.142e-15
Identity = 24/40 (60.00%), Postives = 33/40 (82.50%), Query Frame = 1 
Query:   13 PFTITDATALTQAGYVGEDSESLLYQLLSAADFHVETPQR 132
            PF I DAT+LT+AGYVGED E++L +L+ AADF++E  +R
Sbjct:  137 PFAIADATSLTEAGYVGEDVENILLKLIQAADFNIERAER 176

HSP 2 Score: 46.2098 bits (108), Expect = 7.142e-15
Identity = 26/56 (46.43%), Postives = 35/56 (62.50%), Query Frame = 3 
Query:  174 GENPKNGRKVSGEGISTGLLKMLKRTGINVPPPDNGTRKPPPWENIPIETKDLPFL 341
            GEN    R VSGEG+   LLK+++ T  +VPP   G RK P  E I I+TK++ F+
Sbjct:  191 GENVSITRDVSGEGVQQALLKIIEGTVASVPP--QGGRKHPNQEMIQIDTKNILFI 244
The following BLAST results are available for this feature:
BLAST of EY728351 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
CLPX_BACFR1.820e-1648.21ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_BACFN1.820e-1648.21ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_BACTN1.821e-1648.21ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRSV1.927e-1565.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_PORGI1.927e-1560.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_PORG31.927e-1560.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_CORK42.502e-1565.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRMU4.229e-1562.50ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRTD7.142e-1560.00ATP-dependent Clp protease ATP-binding subunit clp... [more]
CLPX_STRT27.142e-1560.00ATP-dependent Clp protease ATP-binding subunit clp... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-704-002-F01-CT.F Sweet orange fruit, development stadium (5 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728351 ID=EY728351; Name=EY728351; organism=Citrus sinensis; type=EST; length=811bp
CCACGCGTCCGGCCATTTACCATCACTGATGCAACAGCTTTAACGCAGGC
TGGGTATGTTGGAGAAGACTCGGAATCACTATTGTACCAGTTGTTATCGG
CTGCTGATTTCCACGTGGAAACACCTCAGAGACGGAATCGTCTACATTGA
TTAAGTTGAAAAAAATATTGAAAGGTGAAAATCCAAAAAATGGCAGAAAA
GTTTCGGGCGAAGGAATCTCCACAGGCTTACTCAAAATGCTAAAAAGGAC
TGGCATTAATGTTCCCCCACCTGATAACGGAACTCGGAAACCTCCCCCTT
GGGAAAACATTCCAATTGAAACCAAAGACCTGCCTTTCCTATCGTTGTGG
GGCCCTTCGCTGCTTTCCGACAAGCCGACTCCCACAAACCCCCCGAGCCC
TCTGTCCGCGGTCGGGCGCCTGCTCTCCCCCACATGCCAACGCCTTGTCT
GCGGGTACGCCGGTGGCATCCCCGTTGTGTGTCGCTGGATAGGGCAGGCG
TTCGGGTGTTGATGTGCCTGGTCCGGGGGCGCGCGCACGGCCCCTCTACG
TCGCAACGTCCTCTTGTGACTGGCGTCTGCACGCAGTGATTCGTAGTCAT
CCGCAAAGGTGGGGTTTGGGCTTCGGGGCGTGCGGGCACGAGGAGGTGGT
AGCTGTCCCCGGGGTTATCCGGTGTGTGGCGCCCATACGGTCTTCTCCCC
CGGAGCCGATGTGTGTGGCTGAGGATTATCTACTTCTCCGTGTCCCGACC
GCCGGCGTGTACTTGATCTGAGTCGGCGGATGCCACCCCAGGTCTCTCCG
CGCTCCAGGCA
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