EY754218 | Citrus Genome Database

Overview


Name	EY754218
Unique Name	EY754218
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	862

Libraries


Library Name	Type
Sweet orange leaf, field plant A	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: AFC1_ARATH (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana GN=AFC1 PE=2 SV=2)

HSP 1 Score: 259.996 bits (663), Expect = 2.580e-75
Identity = 120/176 (68.18%), Postives = 141/176 (80.11%), Query Frame = 2

Query:  155 METQRIIEFPHKNMDKRPRKRPRLTWDXXXXXXXXXXXXA-----LYCVQEFGNGGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 667
            +ETQR +EFPH+ +DKRPRKRPRLTWD                  LY   EF +G +PN+   +MFY  +PR GSPPWRPDDKDGHYVF +G+ LTPRY+ILSKMGEGTFGQV+ECFDN+ KE+VAIK++RSINKYREAAMIEIDVLQRL R+D+GG+RCVQIRNWFDYRNHICI+
Sbjct:   17 LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIV 192

HSP 2 Score: 42.743 bits (99), Expect = 2.580e-75
Identity = 19/32 (59.38%), Postives = 21/32 (65.62%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPEIFFLFPLSMXKSPDYSF 759
            +MH+LRLIHTDLKPE   L      K PDY F
Sbjct:  230 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKF 261

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: AFC2_ARATH (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana GN=AFC2 PE=1 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 1.490e-63
Identity = 105/177 (59.32%), Postives = 131/177 (74.01%), Query Frame = 2

Query:  155 METQRIIEFPHKNMDKRPRKRPRLTWDXXXXXXXXXXXXALYCVQEFGN------GGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 667
            ME +R+ EFPH +MD+RPRKR RL WD             ++C QE GN       G P+   SS+    + R GSPPWR DDKDGHY+F +G++LTPRY+I SKMGEGTFGQV+EC+D E+KE+VA+KIVR + KYREAAMIEI++LQ+L ++D GG RCVQIRNWFDYRNHICI+
Sbjct:    1 MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQV--GMFCGQEIGNISSFASSGAPSDNSSSLCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIV 175

HSP 2 Score: 39.2762 bits (90), Expect = 1.490e-63
Identity = 17/30 (56.67%), Postives = 20/30 (66.67%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPEIFFLFPLSMXKSPDY 753
            FMH+LR+IHTDLKPE   L      K P+Y
Sbjct:  213 FMHDLRMIHTDLKPENILLVSSDYVKIPEY 242

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: AFC3_ARATH (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana GN=AFC3 PE=2 SV=2)

HSP 1 Score: 175.637 bits (444), Expect = 3.097e-48
Identity = 90/161 (55.90%), Postives = 113/161 (70.19%), Query Frame = 2

Query:  188 KNMDK-RPRKRPRLTWDXXXXXXXXXXXXALYCVQEFGNGGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 667
            ++MDK R RKRPR+TWD                ++  G+ G               R  SPP R DD+DGHYVF++ +NLTPRY+ILSKMGEGTFG+V+EC+D + KE VAIKI+RSI KYR+AAMIEIDVLQ+L ++D G TRCVQ++NWFDYRNHICI+
Sbjct:    7 ESMDKERVRKRPRMTWDEAPAEPEAKRAV----IKGHGSDG---------------RILSPPLRDDDRDGHYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTRCVQMKNWFDYRNHICIV 148

HSP 2 Score: 36.5798 bits (83), Expect = 3.097e-48
Identity = 16/29 (55.17%), Postives = 19/29 (65.52%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPEIFFLFPLSMXKSPD 750
            +MHEL+L+HTDLKPE   L      K PD
Sbjct:  186 YMHELQLVHTDLKPENILLVSSENVKLPD 214

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: LKH1_SCHPO (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe GN=lkh1 PE=1 SV=3)

HSP 1 Score: 99.3673 bits (246), Expect = 1.900e-23
Identity = 46/105 (43.81%), Postives = 66/105 (62.86%), Query Frame = 2

Query:  356 TGSPPWRPDDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARND-IGGTRCVQIRNWFDYRNHICIL 667
            T   P   DD DGHY          RY ++  +G GTFG+V++C+D       AIK+ R+I KYREA++IE+ VLQ +A +D     +C+Q+R++FDYR HICI+
Sbjct:  336 TAFDPSTFDDDDGHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDPTNENKCIQLRDYFDYRKHICIV 440

HSP 2 Score: 29.6462 bits (65), Expect = 1.900e-23
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPE 708
            F+H L L+HTDLKPE
Sbjct:  478 FLHSLGLVHTDLKPE 492

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: CLK3_MOUSE (Dual specificity protein kinase CLK3 OS=Mus musculus GN=Clk3 PE=1 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 5.454e-23
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  286 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 382

HSP 2 Score: 29.261 bits (64), Expect = 5.454e-23
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPE 708
            L F+HE +L HTDLKPE
Sbjct:  419 LRFLHENQLTHTDLKPE 435

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: CLK3_HUMAN (Dual specificity protein kinase CLK3 OS=Homo sapiens GN=CLK3 PE=1 SV=3)

HSP 1 Score: 98.2117 bits (243), Expect = 5.454e-23
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  286 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 382

HSP 2 Score: 29.261 bits (64), Expect = 5.454e-23
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPE 708
            L F+HE +L HTDLKPE
Sbjct:  419 LRFLHENQLTHTDLKPE 435

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: CLK3_RAT (Dual specificity protein kinase CLK3 OS=Rattus norvegicus GN=Clk3 PE=2 SV=2)

HSP 1 Score: 98.2117 bits (243), Expect = 5.480e-23
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  138 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 234

HSP 2 Score: 29.261 bits (64), Expect = 5.480e-23
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPE 708
            L F+HE +L HTDLKPE
Sbjct:  271 LRFLHENQLTHTDLKPE 287

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: CLK3_BOVIN (Dual specificity protein kinase CLK3 OS=Bos taurus GN=CLK3 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 5.480e-23
Identity = 47/97 (48.45%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG+VVEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  138 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 234

HSP 2 Score: 29.261 bits (64), Expect = 5.480e-23
Identity = 12/17 (70.59%), Postives = 14/17 (82.35%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPE 708
            L F+HE +L HTDLKPE
Sbjct:  271 LRFLHENQLTHTDLKPE 287

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: DOA_DROME (Serine/threonine-protein kinase Doa OS=Drosophila melanogaster GN=Doa PE=1 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 2.024e-22
Identity = 43/97 (44.33%), Postives = 70/97 (72.16%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICIL 667
            DD DGH ++  G+ L  RY+I++ +GEGTFG+VV+  D E+   +A+KI++++ KYREAA +EI+ L+++A+ D      CV++ +WFDY  H+CI+
Sbjct:  461 DDADGHLIYHTGDILHHRYKIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYREAAKLEINALEKIAQKDPHCDHLCVKMIDWFDYHGHMCIV 557

HSP 2 Score: 28.8758 bits (63), Expect = 2.024e-22
Identity = 11/15 (73.33%), Postives = 13/15 (86.67%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPE 708
            F+H+ RL HTDLKPE
Sbjct:  595 FLHDNRLTHTDLKPE 609

[Close]

BLAST of EY754218 vs. ExPASy Swiss-Prot
Match: CLK2_HUMAN (Dual specificity protein kinase CLK2 OS=Homo sapiens GN=CLK2 PE=1 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.043e-22
Identity = 44/97 (45.36%), Postives = 69/97 (71.13%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILSKMGEGTFGQVVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICI 664
            DD +GH ++ +G+ L  RY I+S +GEGTFG+VV+C D+ +    VA+KI++++ KY+EAA +EI+VL+++   D      CVQ+ +WFDY  H+CI
Sbjct:  145 DDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCI 241

HSP 2 Score: 27.7202 bits (60), Expect = 2.043e-22
Identity = 10/15 (66.67%), Postives = 13/15 (86.67%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPE 708
            F+H+ +L HTDLKPE
Sbjct:  280 FLHDNKLTHTDLKPE 294

The following BLAST results are available for this feature:

BLAST of EY754218 vs. ExPASy Swiss-Prot

BLAST of EY754218 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42


Match Name	E-value	Identity	Description
AFC1_ARATH	2.580e-75	68.18	Serine/threonine-protein kinase AFC1 OS=Arabidopsi...	[more]
AFC2_ARATH	1.490e-63	59.32	Serine/threonine-protein kinase AFC2 OS=Arabidopsi...	[more]
AFC3_ARATH	3.097e-48	55.90	Serine/threonine-protein kinase AFC3 OS=Arabidopsi...	[more]
LKH1_SCHPO	1.900e-23	43.81	Dual specificity protein kinase lkh1 OS=Schizosacc...	[more]
CLK3_MOUSE	5.454e-23	48.45	Dual specificity protein kinase CLK3 OS=Mus muscul...	[more]
CLK3_HUMAN	5.454e-23	48.45	Dual specificity protein kinase CLK3 OS=Homo sapie...	[more]
CLK3_RAT	5.480e-23	48.45	Dual specificity protein kinase CLK3 OS=Rattus nor...	[more]
CLK3_BOVIN	5.480e-23	48.45	Dual specificity protein kinase CLK3 OS=Bos taurus...	[more]
DOA_DROME	2.024e-22	44.33	Serine/threonine-protein kinase Doa OS=Drosophila ...	[more]
CLK2_HUMAN	2.043e-22	45.36	Dual specificity protein kinase CLK2 OS=Homo sapie...	[more]

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Properties


Property Name	Value
Genbank description	CS12-C1-001-012-B03-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY754218 ID=EY754218; Name=EY754218; organism=Citrus sinensis; type=EST; length=862bp
AATCCCCATTTAGGGTTTCCAACACTGAAAATCATTTCTTCAAAATTTCC
TTAATTTTTTACTCTCAATTTTTTTTAAAGCATTATGATTCAAATTGAGC
TTAATCCATGGCTCTGCTAGGTTTTTTTAATTGAAAATTTAAACAATAAA
AGAAATGGAAACGCAGAGAATTATAGAATTTCCGCATAAAAACATGGACA
AGCGTCCCAGAAAACGACCCCGTTTAACGTGGGACGTTCCGCCTCCTCTT
CCTCCTCCCAAGGTTCTTCCGGCTCTGTATTGTGTTCAGGAGTTTGGGAA
TGGAGGAATGCCCAATTATGCCTGTTCATCCATGTTTTATGGTGCGATTC
CTCGCACCGGATCACCTCCTTGGAGACCTGATGATAAAGACGGACATTAC
GTTTTTGCCATCGGAGAAAATTTAACTCCTCGCTACAGGATTCTCAGCAA
AATGGGTGAAGGGACATTTGGGCAAGTCGTGGAATGTTTTGACAATGAGA
AGAAGGAACTTGTGGCAATCAAAATTGTTCGCTCTATAAACAAGTATCGT
GAAGCAGCCATGATCGAAATTGATGTTCTACAGAGGCTTGCCAGGAATGA
CATTGGTGGCACCCGTTGTGTGCAAATACGGAATTGGTTTGACTATCGTA
ATCATATTTGTATTTTATGCATGAATTACGTCTTATACACACTGACTTGA
AGCCAGAAATATTCTTCTTGTTTCCGCTGAGTATGNTAAAGTCCCCTGAT
TATAGTTTCTATCACGATCGTCAAAGGATGGTTCCTATTTCAGAATCTNC
CCAGTCAAGTGCATTAAGCTAATTGATTTTGAAGCACACATTTGACATCA
GGATACAGCTAT