EY754217 | Citrus Genome Database

Overview


Name	EY754217
Unique Name	EY754217
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	861

Libraries


Library Name	Type
Sweet orange leaf, field plant A	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: AFC1_ARATH (Serine/threonine-protein kinase AFC1 OS=Arabidopsis thaliana GN=AFC1 PE=2 SV=2)

HSP 1 Score: 253.447 bits (646), Expect = 1.390e-79
Identity = 116/176 (65.91%), Postives = 139/176 (78.98%), Query Frame = 2

Query:  155 METHRIIEFPHKNMDKRPKKRPRLTWDXXXXXXXXXXXXA-----LYCVQEFGNGGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 667
            +ET R +EFPH+ +DKRP+KRPRLTWD                  LY   EF +G +PN+   +MFY  +PR GSPPWRPDDKDGHYVF +G+ LTPRY+IL KMGEGTFGQ++ECFDN+ KE+VAIK++RSINKYREAAMIEIDVLQRL R+D+GG+RCVQIRNWFDYRNHICI+
Sbjct:   17 LETQRNVEFPHRIVDKRPRKRPRLTWDAAPPLLPPPPPPTVFQPPLYYGPEFASGLVPNFVYPNMFYNGLPRQGSPPWRPDDKDGHYVFVVGDTLTPRYQILSKMGEGTFGQVLECFDNKNKEVVAIKVIRSINKYREAAMIEIDVLQRLTRHDVGGSRCVQIRNWFDYRNHICIV 192

HSP 2 Score: 63.5438 bits (153), Expect = 1.390e-79
Identity = 28/41 (68.29%), Postives = 34/41 (82.93%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPENILLVSAEYVKVPDYSFYHDRQRMGS 786
            +MH+LRLIHTDLKPENILLVS+EY+K+PDY F     + GS
Sbjct:  230 YMHDLRLIHTDLKPENILLVSSEYIKIPDYKFLSRPTKDGS 270

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: AFC2_ARATH (Serine/threonine-protein kinase AFC2 OS=Arabidopsis thaliana GN=AFC2 PE=1 SV=1)

HSP 1 Score: 218.779 bits (556), Expect = 1.055e-67
Identity = 102/177 (57.63%), Postives = 129/177 (72.88%), Query Frame = 2

Query:  155 METHRIIEFPHKNMDKRPKKRPRLTWDXXXXXXXXXXXXALYCVQEFGN------GGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 667
            ME  R+ EFPH +MD+RP+KR RL WD             ++C QE GN       G P+   SS+    + R GSPPWR DDKDGHY+F +G++LTPRY+I  KMGEGTFGQ++EC+D E+KE+VA+KIVR + KYREAAMIEI++LQ+L ++D GG RCVQIRNWFDYRNHICI+
Sbjct:    1 MEMERVHEFPHTHMDRRPRKRARLGWDVLPQATKAQV--GMFCGQEIGNISSFASSGAPSDNSSSLCVKGVARNGSPPWREDDKDGHYIFELGDDLTPRYKIYSKMGEGTFGQVLECWDRERKEMVAVKIVRGVKKYREAAMIEIEMLQQLGKHDKGGNRCVQIRNWFDYRNHICIV 175

HSP 2 Score: 58.5362 bits (140), Expect = 1.055e-67
Identity = 24/30 (80.00%), Postives = 30/30 (100.00%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPENILLVSAEYVKVPDY 753
            FMH+LR+IHTDLKPENILLVS++YVK+P+Y
Sbjct:  213 FMHDLRMIHTDLKPENILLVSSDYVKIPEY 242

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: AFC3_ARATH (Serine/threonine-protein kinase AFC3 OS=Arabidopsis thaliana GN=AFC3 PE=2 SV=2)

HSP 1 Score: 171.014 bits (432), Expect = 8.376e-52
Identity = 87/161 (54.04%), Postives = 112/161 (69.57%), Query Frame = 2

Query:  188 KNMDK-RPKKRPRLTWDXXXXXXXXXXXXALYCVQEFGNGGMPNYACSSMFYGAIPRTGSPPWRPDDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTRCVQIRNWFDYRNHICIL 667
            ++MDK R +KRPR+TWD                ++  G+ G               R  SPP R DD+DGHYVF++ +NLTPRY+IL KMGEGTFG+++EC+D + KE VAIKI+RSI KYR+AAMIEIDVLQ+L ++D G TRCVQ++NWFDYRNHICI+
Sbjct:    7 ESMDKERVRKRPRMTWDEAPAEPEAKRAV----IKGHGSDG---------------RILSPPLRDDDRDGHYVFSLRDNLTPRYKILSKMGEGTFGRVLECWDRDTKEYVAIKIIRSIKKYRDAAMIEIDVLQKLVKSDKGRTRCVQMKNWFDYRNHICIV 148

HSP 2 Score: 53.1434 bits (126), Expect = 8.376e-52
Identity = 23/29 (79.31%), Postives = 28/29 (96.55%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPENILLVSAEYVKVPD 750
            +MHEL+L+HTDLKPENILLVS+E VK+PD
Sbjct:  186 YMHELQLVHTDLKPENILLVSSENVKLPD 214

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: LKH1_SCHPO (Dual specificity protein kinase lkh1 OS=Schizosaccharomyces pombe GN=lkh1 PE=1 SV=3)

HSP 1 Score: 97.4413 bits (241), Expect = 9.793e-26
Identity = 45/105 (42.86%), Postives = 66/105 (62.86%), Query Frame = 2

Query:  356 TGSPPWRPDDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARND-IGGTRCVQIRNWFDYRNHICIL 667
            T   P   DD DGHY          RY ++  +G GTFG++++C+D       AIK+ R+I KYREA++IE+ VLQ +A +D     +C+Q+R++FDYR HICI+
Sbjct:  336 TAFDPSTFDDDDGHYKVVPNSKFANRYTVVRLLGHGTFGKVIQCYDQSTGRHCAIKVTRAIPKYREASLIELRVLQTIAHSDPTNENKCIQLRDYFDYRKHICIV 440

HSP 2 Score: 39.2762 bits (90), Expect = 9.793e-26
Identity = 16/21 (76.19%), Postives = 19/21 (90.48%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPENILLVS 726
            F+H L L+HTDLKPEN+LLVS
Sbjct:  478 FLHSLGLVHTDLKPENVLLVS 498

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK3_MOUSE (Dual specificity protein kinase CLK3 OS=Mus musculus GN=Clk3 PE=1 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 2.162e-25
Identity = 46/97 (47.42%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG++VEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  286 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 382

HSP 2 Score: 40.0466 bits (92), Expect = 2.162e-25
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPENILLVSAEY 735
            L F+HE +L HTDLKPENIL V++E+
Sbjct:  419 LRFLHENQLTHTDLKPENILFVNSEF 444

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK3_HUMAN (Dual specificity protein kinase CLK3 OS=Homo sapiens GN=CLK3 PE=1 SV=3)

HSP 1 Score: 95.5153 bits (236), Expect = 2.162e-25
Identity = 46/97 (47.42%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG++VEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  286 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 382

HSP 2 Score: 40.0466 bits (92), Expect = 2.162e-25
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPENILLVSAEY 735
            L F+HE +L HTDLKPENIL V++E+
Sbjct:  419 LRFLHENQLTHTDLKPENILFVNSEF 444

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK3_RAT (Dual specificity protein kinase CLK3 OS=Rattus norvegicus GN=Clk3 PE=2 SV=2)

HSP 1 Score: 95.5153 bits (236), Expect = 2.171e-25
Identity = 46/97 (47.42%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG++VEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  138 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 234

HSP 2 Score: 40.0466 bits (92), Expect = 2.171e-25
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPENILLVSAEY 735
            L F+HE +L HTDLKPENIL V++E+
Sbjct:  271 LRFLHENQLTHTDLKPENILFVNSEF 296

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK3_BOVIN (Dual specificity protein kinase CLK3 OS=Bos taurus GN=CLK3 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 2.171e-25
Identity = 46/97 (47.42%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGT-RCVQIRNWFDYRNHICI 664
            DDK+GH V  IG+ L  RY I+  +GEGTFG++VEC D+ + K  VA+KI+R++ KYREAA +EI+VL+++   D      CV + +WF++  H+CI
Sbjct:  138 DDKEGHLVCRIGDWLQERYEIVGNLGEGTFGKVVECLDHARGKSQVALKIIRNVGKYREAARLEINVLKKIKEKDKENKFLCVLMSDWFNFHGHMCI 234

HSP 2 Score: 40.0466 bits (92), Expect = 2.171e-25
Identity = 17/26 (65.38%), Postives = 22/26 (84.62%), Query Frame = 1

Query:  658 LYFMHELRLIHTDLKPENILLVSAEY 735
            L F+HE +L HTDLKPENIL V++E+
Sbjct:  271 LRFLHENQLTHTDLKPENILFVNSEF 296

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: DOA_DROME (Serine/threonine-protein kinase Doa OS=Drosophila melanogaster GN=Doa PE=1 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 3.646e-25
Identity = 42/97 (43.30%), Postives = 69/97 (71.13%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEKKELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICIL 667
            DD DGH ++  G+ L  RY+I+  +GEGTFG++V+  D E+   +A+KI++++ KYREAA +EI+ L+++A+ D      CV++ +WFDY  H+CI+
Sbjct:  461 DDADGHLIYHTGDILHHRYKIMATLGEGTFGRVVKVKDMERDYCMALKIIKNVEKYREAAKLEINALEKIAQKDPHCDHLCVKMIDWFDYHGHMCIV 557

HSP 2 Score: 40.0466 bits (92), Expect = 3.646e-25
Identity = 17/39 (43.59%), Postives = 26/39 (66.67%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPENILLVSAEYVKVPDYSFYHDRQRM 780
            F+H+ RL HTDLKPENIL V ++Y    ++    + +R+
Sbjct:  595 FLHDNRLTHTDLKPENILFVDSDYTSHYNHKINREVRRV 633

[Close]

BLAST of EY754217 vs. ExPASy Swiss-Prot
Match: CLK2_HUMAN (Dual specificity protein kinase CLK2 OS=Homo sapiens GN=CLK2 PE=1 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 1.055e-24
Identity = 42/97 (43.30%), Postives = 68/97 (70.10%), Query Frame = 2

Query:  380 DDKDGHYVFAIGENLTPRYRILIKMGEGTFGQLVECFDNEK-KELVAIKIVRSINKYREAAMIEIDVLQRLARNDIGGTR-CVQIRNWFDYRNHICI 664
            DD +GH ++ +G+ L  RY I+  +GEGTFG++V+C D+ +    VA+KI++++ KY+EAA +EI+VL+++   D      CVQ+ +WFDY  H+CI
Sbjct:  145 DDAEGHLIYHVGDWLQERYEIVSTLGEGTFGRVVQCVDHRRGGARVALKIIKNVEKYKEAARLEINVLEKINEKDPDNKNLCVQMFDWFDYHGHMCI 241

HSP 2 Score: 38.891 bits (89), Expect = 1.055e-24
Identity = 15/24 (62.50%), Postives = 21/24 (87.50%), Query Frame = 1

Query:  664 FMHELRLIHTDLKPENILLVSAEY 735
            F+H+ +L HTDLKPENIL V+++Y
Sbjct:  280 FLHDNKLTHTDLKPENILFVNSDY 303

The following BLAST results are available for this feature:

BLAST of EY754217 vs. ExPASy Swiss-Prot

BLAST of EY754217 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 48


Match Name	E-value	Identity	Description
AFC1_ARATH	1.390e-79	65.91	Serine/threonine-protein kinase AFC1 OS=Arabidopsi...	[more]
AFC2_ARATH	1.055e-67	57.63	Serine/threonine-protein kinase AFC2 OS=Arabidopsi...	[more]
AFC3_ARATH	8.376e-52	54.04	Serine/threonine-protein kinase AFC3 OS=Arabidopsi...	[more]
LKH1_SCHPO	9.793e-26	42.86	Dual specificity protein kinase lkh1 OS=Schizosacc...	[more]
CLK3_MOUSE	2.162e-25	47.42	Dual specificity protein kinase CLK3 OS=Mus muscul...	[more]
CLK3_HUMAN	2.162e-25	47.42	Dual specificity protein kinase CLK3 OS=Homo sapie...	[more]
CLK3_RAT	2.171e-25	47.42	Dual specificity protein kinase CLK3 OS=Rattus nor...	[more]
CLK3_BOVIN	2.171e-25	47.42	Dual specificity protein kinase CLK3 OS=Bos taurus...	[more]
DOA_DROME	3.646e-25	43.30	Serine/threonine-protein kinase Doa OS=Drosophila ...	[more]
CLK2_HUMAN	1.055e-24	43.30	Dual specificity protein kinase CLK2 OS=Homo sapie...	[more]

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Properties


Property Name	Value
Genbank description	CS12-C1-001-012-B02-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY754217 ID=EY754217; Name=EY754217; organism=Citrus sinensis; type=EST; length=861bp
AATCCCCATTTAGGGTTTCCAACACTGAAAAACATTTCTTCAAAATTTCC
TTAATTTTTTACTCTCAATTTTTTTTAAAGCATTATGATTCAAATTGAGC
TTAATCCATGGCTCTGCTAGGTTTTTTTAATTGAAAATTTAAACAATAAA
AGAAATGGAAACGCACAGAATTATAGAATTTCCGCATAAAAACATGGACA
AGCGTCCCAAAAAACGACCCCGTTTAACGTGGGACGTTCCGCCTCCTCTT
CCTCCTCCCAAGGTTCTTCCGGCTCTGTATTGTGTTCAGGAGTTTGGGAA
TGGAGGAATGCCCAATTATGCCTGTTCATCCATGTTTTATGGTGCGATTC
CTCGCACCGGATCACCTCCTTGGAGACCTGATGATAAAGACGGACATTAC
GTTTTTGCCATCGGAGAAAATTTAACTCCTCGCTACAGGATTCTCATCAA
AATGGGTGAAGGGACATTTGGGCAACTCGTGGAATGTTTTGACAATGAGA
AGAAGGAACTTGTGGCAATCAAAATTGTTCGCTCTATAAACAAGTATCGT
GAAGCAGCCATGATCGAAATTGATGTTCTACAGAGGCTTGCCAGGAATGA
CATTGGTGGCACCCGTTGTGTGCAAATACGGAATTGGTTTGACTATCGTA
ATCATATCTGTATTTTATGCATGAATTACGTCTTATACACACTGACTTGA
AGCCAGAAAATATTCTTCTTGTTTCCGCTGAGTATGTTAAAGTCCCTGAT
TATAGTTTCTATCACGATCGTCAAAGGATGGGTTCTATTTCAGGATCTTC
CCAGTGCAGTGCATTAAGCTAATTGATTTTGGAGCACACATTTGACCTCA
GGATCCAGCTT