EY728012

Overview
NameEY728012
Unique NameEY728012
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1047
Libraries
Library NameType
Sweet orange fruit, development stadium (4 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: ASPRX_ORYSJ (Aspartic proteinase OS=Oryza sativa subsp. japonica GN=RAP PE=2 SV=2)

HSP 1 Score: 128.642 bits (322), Expect = 9.944e-32
Identity = 64/141 (45.39%), Postives = 89/141 (63.12%), Query Frame = 2
Query:   74 EQKLLRSVFCLWVLASCLLLPASSNXXXXXXXXXXXXXXHSLNAARITRKERYMGGAGVSGVRHRLGDSDEDILPLKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCYFSISCYFHSRYKSRKSNTYTEIGKS 496
            ++ LL    CLW L+  LLL ASS+                L AA++ ++   +          + G SD D +PL ++++ QY+G IG+GSPPQNF+VIFDTGSSNLWVPS+KCYFSI+CY HSRY S+KS++Y   G++
Sbjct:    3 KRHLLLVTTCLWALSCALLLHASSDGFLRVNLNKKRLDKEDLTAAKLAQQGNRL---------LKTGSSDSDPVPLVDYLNTQYYGVIGLGSPPQNFTVIFDTGSSNLWVPSAKCYFSIACYLHSRYNSKKSSSYKADGET 134          

HSP 2 Score: 30.0314 bits (66), Expect = 9.944e-32
Identity = 21/41 (51.22%), Postives = 27/41 (65.85%), Query Frame = 3
Query:  510 TMGSGIISGFFSQNNV*SWGMFGVKDQVFP*GYTKKGSLTF 632
            T GSG ISGFFS++NV   G   VK+Q F    T++ S+TF
Sbjct:  138 TYGSGAISGFFSKDNV-LVGDLVVKNQKFI-EATRETSVTF 176          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: CYPR1_CYNCA (Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO1 PE=1 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 1.071e-30
Identity = 57/81 (70.37%), Postives = 68/81 (83.95%), Query Frame = 2
Query:  254 GVRHRLGDSDEDILPLKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCYFSISCYFHSRYKSRKSNTYTEIGKS 496
            GVR    DSD +++ LKN+MDAQYFGEIGIG+PPQ F+VIFDTGSSNLWVPSSKCYFS++C FHS+Y+S  S TY + GKS
Sbjct:   28 GVRGNFRDSDGELIALKNYMDAQYFGEIGIGTPPQKFTVIFDTGSSNLWVPSSKCYFSVACLFHSKYRSTDSTTYKKNGKS 108          

HSP 2 Score: 27.335 bits (59), Expect = 1.071e-30
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV*SWGMFGVKDQVFP*GYTKKGSLTF 632
            G+G ISGFFSQ++V   G   VK+Q F    TK+  +TF
Sbjct:  114 GTGSISGFFSQDSV-KLGDLLVKEQDFI-EATKEPGITF 150          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: ASPR1_ORYSJ (Aspartic proteinase oryzasin-1 OS=Oryza sativa subsp. japonica GN=Os05g0567100 PE=2 SV=2)

HSP 1 Score: 123.635 bits (309), Expect = 1.151e-29
Identity = 60/99 (60.61%), Postives = 79/99 (79.80%), Query Frame = 2
Query:  203 AARITRKE--RYMGGAGVSGVRHRLGDSDEDILPLKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCYFSISCYFHSRYKSRKSNTYTEIGK 493
            AAR++ +E  R +G  G + +    G  + DI+ LKN+M+AQYFGEIG+G+PPQ F+VIFDTGSSNLWVPS+KCYFSI+C+FHSRYKS +S+TY + GK
Sbjct:   45 AARLSGEEGARRLGLRGANSLGG--GGGEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACFFHSRYKSGQSSTYQKNGK 141          

HSP 2 Score: 28.1054 bits (61), Expect = 1.151e-29
Identity = 19/39 (48.72%), Postives = 26/39 (66.67%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV*SWGMFGVKDQVFP*GYTKKGSLTF 632
            G+G I+GFFS+++V + G   VKDQ F    TK+  LTF
Sbjct:  148 GTGSIAGFFSEDSV-TVGDLVVKDQEFI-EATKEPGLTF 184          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: ASPR_HORVU (Phytepsin OS=Hordeum vulgare PE=1 SV=1)

HSP 1 Score: 120.168 bits (300), Expect = 4.628e-28
Identity = 53/82 (64.63%), Postives = 67/82 (81.71%), Query Frame = 2
Query:  248 VSGVRHRLGDSDEDILPLKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCYFSISCYFHSRYKSRKSNTYTEIGK 493
            +SG      + + DI+ LKN+M+AQYFGEIG+G+PPQ F+VIFDTGSSNLWVPS+KCYFSI+CY HSRYK+  S+TY + GK
Sbjct:   59 LSGANPLRSEEEGDIVALKNYMNAQYFGEIGVGTPPQKFTVIFDTGSSNLWVPSAKCYFSIACYLHSRYKAGASSTYKKNGK 140          

HSP 2 Score: 26.1794 bits (56), Expect = 4.628e-28
Identity = 17/39 (43.59%), Postives = 26/39 (66.67%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV*SWGMFGVKDQVFP*GYTKKGSLTF 632
            G+G I+G+FS+++V + G   VKDQ F    TK+  +TF
Sbjct:  147 GTGSIAGYFSEDSV-TVGDLVVKDQEFI-EATKEPGITF 183          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: ASPR_CUCPE (Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 1.638e-24
Identity = 59/136 (43.38%), Postives = 86/136 (63.24%), Query Frame = 2
Query:  101 CLWVLASCLLLPASSNXXXXXXXXXXXXXX-HSLNAARITRKERYMGGAGVS--GVRHRLGDS-DEDILPLKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCYFSISCYFHSRYKSRKSNTYTEIGKS 496
            CL++L S  ++ ++SN                +  AAR+  K+  +  A       +  LG+S D DI+ LKN++DAQY+GEI IG+PPQ F+VIFDTGSSNLWV   +C FS++C+FH+RYKS +S++Y + G S
Sbjct:   12 CLFLLVSFNIVSSASNDGLLRVGLKKIKLDPENRLAARVESKDAEILKAAFRKYNPKGNLGESSDTDIVALKNYLDAQYYGEIAIGTPPQKFTVIFDTGSSNLWV-LCECLFSVACHFHARYKSSRSSSYKKNGTS 146          

HSP 2 Score: 30.8018 bits (68), Expect = 1.638e-24
Identity = 20/39 (51.28%), Postives = 26/39 (66.67%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV*SWGMFGVKDQVFP*GYTKKGSLTF 632
            G+G +SGFFS +NV   G   VK+QVF    T++ SLTF
Sbjct:  152 GTGAVSGFFSYDNV-KVGDLVVKEQVFI-EATREPSLTF 188          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: CATD_CHICK (Cathepsin D OS=Gallus gallus GN=CTSD PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 2.143e-20
Identity = 42/65 (64.62%), Postives = 52/65 (80.00%), Query Frame = 2
Query:  299 LKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCY-FSISCYFHSRYKSRKSNTYTEIG 490
            LKN+MDAQY+GEIGIG+PPQ F+V+FDTGSSNLWVPS  C+   I+C  H +Y + KS+TY E G
Sbjct:   70 LKNYMDAQYYGEIGIGTPPQKFTVVFDTGSSNLWVPSVHCHLLDIACLLHHKYDASKSSTYVENG 134          

HSP 2 Score: 25.7942 bits (55), Expect = 2.143e-20
Identity = 12/27 (44.44%), Postives = 19/27 (70.37%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV*SWGMFGVKDQVF 596
            G+G +SGF SQ+ V + G   +K+Q+F
Sbjct:  142 GTGSLSGFLSQDTV-TLGNLKIKNQIF 167          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: CATD_HUMAN (Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1)

HSP 1 Score: 94.7449 bits (234), Expect = 5.015e-19
Identity = 43/68 (63.24%), Postives = 54/68 (79.41%), Query Frame = 2
Query:  299 LKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKC-YFSISCYFHSRYKSRKSNTYTEIGKSW 499
            LKN+MDAQY+GEIGIG+PPQ F+V+FDTGSSNLWVPS  C    I+C+ H +Y S KS+TY + G S+
Sbjct:   71 LKNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSDKSSTYVKNGTSF 138          

HSP 2 Score: 21.1718 bits (43), Expect = 5.015e-19
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV 557
            GSG +SG+ SQ+ V
Sbjct:  143 GSGSLSGYLSQDTV 156          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: CARP_NEUCR (Vacuolar protease A OS=Neurospora crassa GN=pep-4 PE=3 SV=2)

HSP 1 Score: 94.7449 bits (234), Expect = 9.271e-19
Identity = 52/121 (42.98%), Postives = 76/121 (62.81%), Query Frame = 2
Query:  293 LPLKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKCYFSISCYFHSRYKSRKSNTYTEIGKSW*K*NYGIWDNFWLLQSKQCLKLGDVWCQGSGFSLRLHKKGKSHFFLLVKGLM 655
            +P+ NFM+AQYF EI IG+PPQ F V+ DTGSSNLWVPSS+C  SI+CY H++Y+S +S+TY + G S+ K  YG       + S+  + +GD+      F+    + G +  F    G++
Sbjct:   75 VPITNFMNAQYFSEITIGTPPQTFKVVLDTGSSNLWVPSSQC-GSIACYLHNKYESSESSTYKKNGTSF-KIEYGSGSLSGFV-SQDRMTIGDITINDQLFAEATSEPGLAFAFGRFDGIL 192          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: CATD_CANFA (Cathepsin D OS=Canis familiaris GN=CTSD PE=2 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.103e-18
Identity = 42/68 (61.76%), Postives = 54/68 (79.41%), Query Frame = 2
Query:  299 LKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKC-YFSISCYFHSRYKSRKSNTYTEIGKSW 499
            L+N+MDAQY+GEIGIG+PPQ F+V+FDTGSSNLWVPS  C    I+C+ H +Y S KS+TY + G S+
Sbjct:   71 LRNYMDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWIHHKYNSGKSSTYVKNGTSF 138          

HSP 2 Score: 21.1718 bits (43), Expect = 1.103e-18
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV 557
            GSG +SG+ SQ+ V
Sbjct:  143 GSGSLSGYLSQDTV 156          
BLAST of EY728012 vs. ExPASy Swiss-Prot
Match: CATD_RAT (Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.434e-18
Identity = 42/68 (61.76%), Postives = 54/68 (79.41%), Query Frame = 2
Query:  299 LKNFMDAQYFGEIGIGSPPQNFSVIFDTGSSNLWVPSSKC-YFSISCYFHSRYKSRKSNTYTEIGKSW 499
            LKN++DAQY+GEIGIG+PPQ F+V+FDTGSSNLWVPS  C    I+C+ H +Y S KS+TY + G S+
Sbjct:   71 LKNYLDAQYYGEIGIGTPPQCFTVVFDTGSSNLWVPSIHCKLLDIACWVHHKYNSDKSSTYVKNGTSF 138          

HSP 2 Score: 21.1718 bits (43), Expect = 1.434e-18
Identity = 8/14 (57.14%), Postives = 11/14 (78.57%), Query Frame = 3
Query:  516 GSGIISGFFSQNNV 557
            GSG +SG+ SQ+ V
Sbjct:  143 GSGSLSGYLSQDTV 156          
The following BLAST results are available for this feature:
BLAST of EY728012 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 84
Match NameE-valueIdentityDescription
ASPRX_ORYSJ9.944e-3245.39Aspartic proteinase OS=Oryza sativa subsp. japonic... [more]
CYPR1_CYNCA1.071e-3070.37Cyprosin (Fragment) OS=Cynara cardunculus GN=CYPRO... [more]
ASPR1_ORYSJ1.151e-2960.61Aspartic proteinase oryzasin-1 OS=Oryza sativa sub... [more]
ASPR_HORVU4.628e-2864.63Phytepsin OS=Hordeum vulgare PE=1 SV=1[more]
ASPR_CUCPE1.638e-2443.38Aspartic proteinase OS=Cucurbita pepo PE=2 SV=1[more]
CATD_CHICK2.143e-2064.62Cathepsin D OS=Gallus gallus GN=CTSD PE=1 SV=1[more]
CATD_HUMAN5.015e-1963.24Cathepsin D OS=Homo sapiens GN=CTSD PE=1 SV=1[more]
CARP_NEUCR9.271e-1942.98Vacuolar protease A OS=Neurospora crassa GN=pep-4 ... [more]
CATD_CANFA1.103e-1861.76Cathepsin D OS=Canis familiaris GN=CTSD PE=2 SV=1[more]
CATD_RAT1.434e-1861.76Cathepsin D OS=Rattus norvegicus GN=Ctsd PE=1 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-703-109-D02-CT.F Sweet orange fruit, development stadium (4 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY728012 ID=EY728012; Name=EY728012; organism=Citrus sinensis; type=EST; length=1047bp
CCCACGCGTCCGCCCACGCGTCCGCCCACGCGTCCGCAAACTCAGCTTCA
GCAGAAAACCAAAGAACATCATGGAGCAGAAACTTCTTCGGTCAGTTTTC
TGTTTATGGGTGCTAGCGTCGTGCTTGCTTCTTCCTGCTTCCTCTAATGG
CTTACGGAGAATTGGCTTGAAGAAGCGGCGACTAGATCTTCATAGTCTAA
ATGCTGCAAGAATAACAAGAAAAGAGCGATATATGGGGGGAGCAGGTGTT
AGTGGCGTGCGCCATCGTTTGGGTGATTCAGATGAAGATATATTGCCTTT
GAAGAACTTTATGGATGCTCAATACTTTGGAGAGATTGGTATTGGCTCTC
CCCCACAAAATTTCAGTGTCATATTTGATACCGGCAGTTCCAATCTCTGG
GTTCCATCTTCAAAGTGCTATTTCTCTATTTCCTGCTATTTCCATTCGAG
GTACAAGTCCAGAAAATCCAATACATATACCGAAATTGGAAAATCCTGGT
GAAAATAAAACTATGGGATCTGGGATAATTTCTGGCTTCTTCAGTCAAAA
CAATGTTTAAAGTTGGGGGATGTTTGGTGTCAAGGATCAGGTTTTTCCTT
GAGGCTACACAAAAAAGGGAAGTCTCACTTTTTTCTATTGGTCAAGGGTT
TGATGGAAATAAAAAGGCCTTGGGGTTCCGGGAAAATTTGCAATTGGGGA
AAGCTGTGCCTGGTGGGGGGAAAAATATGGGTTAGAACAGGGGCCTTTTG
AAACCGAAGGAAGGGCTTTACCCATTTCTTGGCTCTAACAGGGAGATCCA
AAACCCTTAAATAAGGGGGGGAAAAAATTTTTTTTTTGGTGGGGGTGATT
TGTATCCAAAAATACTTTTTAAAGAGGGAAAAACTTCTCCCTTTGGATTC
CCGTATGTACATGAAAAAAAAAGGGATCCAGGGGAAGGATCTACCAATAT
GGTGAGGATAAAAAATTATTTCAACACGAATGGAGAAGAACACACCCACC
TACCCCCCATGACGATATAAGGTATACTTAAAGACGGAAAGGTGAGT
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