EY753369 | Citrus Genome Database

Overview


Name	EY753369
Unique Name	EY753369
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	898

Libraries


Library Name	Type
Sweet orange leaf, field plant A	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY753369_ssr40	microsatellite	EY753369_ssr40:40..65.	BLAST: Citrus ESTs to Prunus persica proteins V1
EY753369_ssr637	microsatellite	EY753369_ssr637:637..648.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: OXI1_ARATH (Serine/threonine-protein kinase OXI1 OS=Arabidopsis thaliana GN=OXI1 PE=2 SV=1)

HSP 1 Score: 119.398 bits (298), Expect = 2.805e-26
Identity = 63/148 (42.57%), Postives = 89/148 (60.14%), Query Frame = 3

Query:  120 SLDLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAE-------RRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            +LD + L+ L +LG+GA G VFLV D  A        ALKV+ K  +  K +         +R  +E  VLSR  HP  P L G L TD+ + +A+ YCPG +LN LR  Q++ +FS  +IRFY +E++ A+++LHN GI YR LKP+
Sbjct:   11 ALDFNRLEVLSLLGRGAKGVVFLVRDDDAKL-----LALKVILKEAIEKKKKGRESEDDEYKRVSFEQGVLSRFDHPLFPSLHGVLATDKVIGYAIDYCPGQNLNSLRKMQSESMFSDEIIRFYAAELVLALDYLHNQGIVYRDLKPD 153

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: G11A_ORYSI (Protein kinase G11A OS=Oryza sativa subsp. indica GN=OsI_021818 PE=2 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 8.059e-26
Identity = 60/140 (42.86%), Postives = 86/140 (61.43%), Query Frame = 3

Query:  123 LDLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            L L + K LK LG G +G+V+L          +  FA+KV+DK++L A  +   RA+ E  +L  L HPFLP L    ETD+F    + +CPGGDL+ LR +Q  + F    ++FY++EI+ A+E+LH +GI YR LKPE
Sbjct:  190 LGLSHFKLLKKLGCGDIGSVYLSELNGT----KSYFAMKVMDKASL-ASRKKLLRAQTEKEILQCLDHPFLPTLYTHFETDKFSCLVMEFCPGGDLHTLRQRQRGKYFPEQAVKFYVAEILLAMEYLHMLGIIYRDLKPE 324

HSP 2 Score: 28.1054 bits (61), Expect = 8.059e-26
Identity = 10/18 (55.56%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLS 595
            N+L+R+  H+ L+DFDLS
Sbjct:  325 NVLVREDGHIMLSDFDLS 342

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: G11A_ORYSJ (Protein kinase G11A OS=Oryza sativa subsp. japonica GN=Os06g0291600 PE=2 SV=1)

HSP 1 Score: 110.153 bits (274), Expect = 1.049e-25
Identity = 60/140 (42.86%), Postives = 86/140 (61.43%), Query Frame = 3

Query:  123 LDLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            L L + K LK LG G +G+V+L          +  FA+KV+DK++L A  +   RA+ E  +L  L HPFLP L    ETD+F    + +CPGGDL+ LR +Q  + F    ++FY++EI+ A+E+LH +GI YR LKPE
Sbjct:  190 LGLSHFKLLKKLGCGDIGSVYLSELSGT----KSYFAMKVMDKASL-ASRKKLLRAQTEKEILQCLDHPFLPTLYTHFETDKFSCLVMEFCPGGDLHTLRQRQRGKYFPEQAVKFYVAEILLAMEYLHMLGIIYRDLKPE 324

HSP 2 Score: 28.1054 bits (61), Expect = 1.049e-25
Identity = 10/18 (55.56%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLS 595
            N+L+R+  H+ L+DFDLS
Sbjct:  325 NVLVREDGHIMLSDFDLS 342

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: KPK1_PHAVU (Protein kinase PVPK-1 OS=Phaseolus vulgaris PE=2 SV=1)

HSP 1 Score: 107.457 bits (267), Expect = 3.915e-25
Identity = 58/140 (41.43%), Postives = 87/140 (62.14%), Query Frame = 3

Query:  123 LDLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            L++ + + LK LG G +G+V+L    A     R  FA+KV++K+ L A  +   RA+ E  +L  L HPFLP L    ET+ F    + +CPGGDL+ LR +Q  + FS   +RFY++E++ ++E+LH +GI YR LKPE
Sbjct:  224 LEMRHFRLLKKLGCGDIGSVYL----AELSGTRTSFAMKVMNKTEL-ANRKKLLRAQTEREILQSLDHPFLPTLYTHFETEIFSCLVMEFCPGGDLHALRQRQPGKYFSEHAVRFYVAEVLLSLEYLHMLGIIYRDLKPE 358

HSP 2 Score: 28.8758 bits (63), Expect = 3.915e-25
Identity = 13/33 (39.39%), Postives = 18/33 (54.55%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLSRNLVKKTAFEKQSLN 640
            N+L+R+  H+ L+DFDLS          K S N
Sbjct:  359 NVLVREDGHIMLSDFDLSLRCSVSPTLVKSSNN 391

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: KPK7_ARATH (Putative serine/threonine-protein kinase PK7 OS=Arabidopsis thaliana GN=PK7 PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.036e-23
Identity = 56/141 (39.72%), Postives = 83/141 (58.87%), Query Frame = 3

Query:  120 SLDLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            SL+  + K +K LG G +G V+L        +    FA+KV++K+ + A+ +  R A+ E  +L  L HPFLP L    ET+      + +CPGGDL+ LR KQ  + F     RFY++E++ A+E+LH +GI YR LKPE
Sbjct:  176 SLEAKDFKLIKKLGGGDIGNVYLAELIGTGVS----FAVKVMEKAAIAARKKLVR-AQTEKEILQSLDHPFLPTLYSHFETEMNSCLVMEFCPGGDLHSLRQKQRGKYFPEQAARFYVAEVLLAMEYLHMLGIIYRDLKPE 311

HSP 2 Score: 28.1054 bits (61), Expect = 2.036e-23
Identity = 10/18 (55.56%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLS 595
            N+L+R+  H+ L+DFDLS
Sbjct:  312 NVLVREDGHIMLSDFDLS 329

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: PHOT1_ARATH (Phototropin-1 OS=Arabidopsis thaliana GN=PHOT1 PE=1 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 2.006e-19
Identity = 53/140 (37.86%), Postives = 78/140 (55.71%), Query Frame = 3

Query:  123 LDLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            + L + K +K LG G  G+V LV     D      FA+K +DK+ +  +++  R AR E  +L  L HPFLP L  S +T   +     Y PGG+L +L  +Q  +V     +RFY ++++ A+E+LH  GI YR LKPE
Sbjct:  658 IGLKHFKPVKPLGSGDTGSVHLVELVGTDQL----FAMKAMDKAVMLNRNKVHR-ARAEREILDLLDHPFLPALYASFQTKTHICLITDYYPGGELFMLLDRQPRKVLKEDAVRFYAAQVVVALEYLHCQGIIYRDLKPE 792

HSP 2 Score: 23.0978 bits (48), Expect = 2.006e-19
Identity = 9/18 (50.00%), Postives = 15/18 (83.33%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLS 595
            N+LI+ +  ++L+DFDLS
Sbjct:  793 NVLIQGNGDISLSDFDLS 810

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: KS6A6_DANRE (Ribosomal protein S6 kinase alpha-6 OS=Danio rerio GN=rps6ka6 PE=2 SV=1)

HSP 1 Score: 87.4261 bits (215), Expect = 2.144e-18
Identity = 50/139 (35.97%), Postives = 81/139 (58.27%), Query Frame = 3

Query:  126 DLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            D    + LK+LG+G+ G VFLV  +   P A   +A+KV+ K++L  +     R + E  +L  ++HPF+ KL  + +T+  L   + +  GGD  V      + +F+   ++FYL+E+  A++HLHN+GI YR LKPE
Sbjct:   63 DPSQFELLKVLGQGSFGKVFLVR-KLMGPDAGQLYAMKVLKKASLKVRDRV--RTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGD--VFTRLSKEVMFTEEDVKFYLAELALALDHLHNLGIVYRDLKPE 196

HSP 2 Score: 26.1794 bits (56), Expect = 2.144e-18
Identity = 11/22 (50.00%), Postives = 16/22 (72.73%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLSRNLV 607
            NIL+ ++ H+ LTDF LS+  V
Sbjct:  197 NILLDEAGHIKLTDFGLSKESV 218

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: KS6A3_MOUSE (Ribosomal protein S6 kinase alpha-3 OS=Mus musculus GN=Rps6ka3 PE=1 SV=2)

HSP 1 Score: 87.0409 bits (214), Expect = 4.712e-18
Identity = 51/139 (36.69%), Postives = 83/139 (59.71%), Query Frame = 3

Query:  126 DLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            D    + LK+LG+G+ G VFLV  + +   AR  +A+KV+ K+TL  +     R + E  +L  ++HPF+ KL  + +T+  L   + +  GGDL     K+   +F+   ++FYL+E+  A++HLH++GI YR LKPE
Sbjct:   64 DPSQFELLKVLGQGSFGKVFLVK-KISGSDARQLYAMKVLKKATLKVRDRV--RTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEV--MFTEEDVKFYLAELALALDHLHSLGIIYRDLKPE 197

HSP 2 Score: 25.409 bits (54), Expect = 4.712e-18
Identity = 10/22 (45.45%), Postives = 15/22 (68.18%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLSRNLV 607
            NIL+ +  H+ LTDF LS+  +
Sbjct:  198 NILLDEEGHIKLTDFGLSKESI 219

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: KS6A3_HUMAN (Ribosomal protein S6 kinase alpha-3 OS=Homo sapiens GN=RPS6KA3 PE=1 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 4.712e-18
Identity = 51/139 (36.69%), Postives = 83/139 (59.71%), Query Frame = 3

Query:  126 DLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            D    + LK+LG+G+ G VFLV  + +   AR  +A+KV+ K+TL  +     R + E  +L  ++HPF+ KL  + +T+  L   + +  GGDL     K+   +F+   ++FYL+E+  A++HLH++GI YR LKPE
Sbjct:   64 DPSQFELLKVLGQGSFGKVFLVK-KISGSDARQLYAMKVLKKATLKVRDRV--RTKMERDILVEVNHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEV--MFTEEDVKFYLAELALALDHLHSLGIIYRDLKPE 197

HSP 2 Score: 25.409 bits (54), Expect = 4.712e-18
Identity = 10/22 (45.45%), Postives = 15/22 (68.18%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLSRNLV 607
            NIL+ +  H+ LTDF LS+  +
Sbjct:  198 NILLDEEGHIKLTDFGLSKESI 219

[Close]

BLAST of EY753369 vs. ExPASy Swiss-Prot
Match: KS6A1_CAEEL (Putative ribosomal protein S6 kinase alpha-1 OS=Caenorhabditis elegans GN=rskn-1 PE=1 SV=4)

HSP 1 Score: 87.8113 bits (216), Expect = 7.955e-18
Identity = 51/139 (36.69%), Postives = 82/139 (58.99%), Query Frame = 3

Query:  126 DLDNLKALKILGKGAMGTVFLVHDRAADPTARCPFALKVVDKSTLHAKHEAERRARWEIRVLSRLSHPFLPKLLGSLETDEFLAWAVPYCPGGDLNVLRYKQNDRVFSPSVIRFYLSEIICAIEHLHNMGIAYRXLKPE 542
            D    + LK+LG+G+ G VFLV       +    +A+KV+ K+TL  +    +R + E  +L+ +SHPF+ KL  + +T+  L   + +  GGDL     K+   +F+   ++FYL+E+  A+EHLH++GI YR LKPE
Sbjct:  100 DPRQFELLKVLGQGSFGKVFLVRKVRGRDSGHV-YAMKVLKKATLKVRDR--QRTKLERNILAHISHPFIVKLHYAFQTEGKLYLILDFLRGGDLFTRLSKEV--MFTEDDVKFYLAELTLALEHLHSLGIVYRDLKPE 233

HSP 2 Score: 23.8682 bits (50), Expect = 7.955e-18
Identity = 12/28 (42.86%), Postives = 17/28 (60.71%), Query Frame = 2

Query:  542 NILIRQSVHVTLTDFDLSRNLV---KKT 616
            NIL+    H+ +TDF LS+  +   KKT
Sbjct:  234 NILLDADGHIKVTDFGLSKEAIDSEKKT 261

The following BLAST results are available for this feature:

BLAST of EY753369 vs. ExPASy Swiss-Prot

BLAST of EY753369 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 154


Match Name	E-value	Identity	Description
OXI1_ARATH	2.805e-26	42.57	Serine/threonine-protein kinase OXI1 OS=Arabidopsi...	[more]
G11A_ORYSI	8.059e-26	42.86	Protein kinase G11A OS=Oryza sativa subsp. indica ...	[more]
G11A_ORYSJ	1.049e-25	42.86	Protein kinase G11A OS=Oryza sativa subsp. japonic...	[more]
KPK1_PHAVU	3.915e-25	41.43	Protein kinase PVPK-1 OS=Phaseolus vulgaris PE=2 S...	[more]
KPK7_ARATH	2.036e-23	39.72	Putative serine/threonine-protein kinase PK7 OS=Ar...	[more]
PHOT1_ARATH	2.006e-19	37.86	Phototropin-1 OS=Arabidopsis thaliana GN=PHOT1 PE=...	[more]
KS6A6_DANRE	2.144e-18	35.97	Ribosomal protein S6 kinase alpha-6 OS=Danio rerio...	[more]
KS6A3_MOUSE	4.712e-18	36.69	Ribosomal protein S6 kinase alpha-3 OS=Mus musculu...	[more]
KS6A3_HUMAN	4.712e-18	36.69	Ribosomal protein S6 kinase alpha-3 OS=Homo sapien...	[more]
KS6A1_CAEEL	7.955e-18	36.69	Putative ribosomal protein S6 kinase alpha-1 OS=Ca...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS12-C1-001-002-A04-CT.F Sweet orange leaf, field plant A Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY753369 ID=EY753369; Name=EY753369; organism=Citrus sinensis; type=EST; length=898bp
TAAAAGAGGCTGACCCACGCGTCCGCTCACTTGTAGTTTTCTCTCTCTCT
CTCTCTCTCTCTCTCTTTCTTTCTCAAATTATGGAGGCAACTCCACCACC
TCAGCCGCAGACGACGCCGTCTTTAGACCTCGACAACCTCAAAGCTCTAA
AAATCCTCGGCAAAGGCGCGATGGGCACGGTCTTCTTAGTCCACGACCGG
GCAGCTGACCCAACTGCCCGCTGCCCGTTCGCTCTCAAGGTTGTCGACAA
ATCTACCCTCCATGCAAAACACGAAGCTGAAAGACGCGCCCGCTGGGAAA
TCCGGGTCCTGTCCCGGCTCTCCCACCCGTTTCTCCCGAAGCTTCTCGGA
TCTTTAGAAACCGACGAGTTCCTCGCCTGGGCCGTCCCTTATTGCCCCGG
TGGCGACCTCAATGTCCTCCGTTACAAGCAAAACGACCGCGTTTTCTCCC
CTTCCGTCATCCGCTTTTACTTGTCAGAAATCATTTGCGCCATCGAGCAT
CTCCACAACATGGGCATTGCTTACAGAGANTTGAAACCCGAAACATTTTG
ATTCGGCAGTCGGTTCACGTGACTCTCACCGACTTCGACCTCTCTCGTAA
CCTAGTTAAGAAGACCGCCTTTGAGAAACAATCCCTTAATAATAATAAGT
TCCCGGGACTTCCAGTTCCCGGAGATCCCTAACGACCCGGAGAATTCTAA
ACTGAGGGCTCCCGCTTTACCCGAGAACAAACCGGGTTGAAGAGACAAAA
TCGCCCGGTCACCCCATTGGGTCCAGGCACAGAGTTCCCACCACGGGGAC
GCTCCACTCGTTGGCGGACCGAAAATTCCGGTCCCCGGAGTATCCGGGGG
GAGGCCCCAATCCCGTTGGATGGGGGCTTGGGAATATAACAAAAAAAA