EY753062

Overview
NameEY753062
Unique NameEY753062
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length942
Libraries
Library NameType
Sweet orange flower, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP25_ARATH (Probable aquaporin PIP2-5 OS=Arabidopsis thaliana GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 166.392 bits (420), Expect = 2.307e-43
Identity = 80/110 (72.73%), Postives = 85/110 (77.27%), Query Frame = 3 
Query:  147 VAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            V +  S+S KDY DPPPEPLFDA EL KWSFYRALIAEFIA            IGYKSQTDPA N D C GVG+LGIAWAFGGMIF+LVYCTAGISGGHINPAVTF  +L
Sbjct:    6 VGDKRSFSGKDYQDPPPEPLFDATELGKWSFYRALIAEFIATLLFLYVTIMTVIGYKSQTDPALNPDQCTGVGVLGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLLL 115

HSP 2 Score: 27.335 bits (59), Expect = 2.307e-43
Identity = 19/38 (50.00%), Postives = 20/38 (52.63%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKKRTGGGDNKL 616
            AVMYMVAQCL AI    +   F       R GGG N L
Sbjct:  124 AVMYMVAQCLGAICGVALVKAFQSAY-FTRYGGGANGL 160

HSP 3 Score: 24.6386 bits (52), Expect = 2.307e-43
Identity = 11/13 (84.62%), Postives = 12/13 (92.31%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGL LARKV+LVR
Sbjct:  111 FGLLLARKVTLVR 123
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP23_ORYSJ (Probable aquaporin PIP2-3 OS=Oryza sativa subsp. japonica GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 2.767e-40
Identity = 78/121 (64.46%), Postives = 85/121 (70.25%), Query Frame = 3 
Query:  129 MAKD---TGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNV--DGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            MAKD      AE G Y AKDY+DPPP PL DA ELTKWS YRA+IAEF+A            IGYK Q+DP  N     C+GVGILGIAWAFGGMIF+LVYCTAG+SGGHINPAVTF   L
Sbjct:    1 MAKDIEAAAAAEGGEYMAKDYSDPPPAPLIDAEELTKWSLYRAVIAEFVATLLFLYITVATVIGYKHQSDPGANAADAACSGVGILGIAWAFGGMIFILVYCTAGVSGGHINPAVTFGLFL 121

HSP 2 Score: 28.1054 bits (61), Expect = 2.767e-40
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSLVR
Sbjct:  117 FGLFLARKVSLVR 129

HSP 3 Score: 25.0238 bits (53), Expect = 2.767e-40
Identity = 17/38 (44.74%), Postives = 21/38 (55.26%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKKRTGGGDNKL 616
            AV+Y+VAQ L AI    +  GF       R GGG N+L
Sbjct:  130 AVLYIVAQSLGAICGVGLVKGFQSAF-YVRYGGGANEL 166
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP22_ORYSJ (Probable aquaporin PIP2-2 OS=Oryza sativa subsp. japonica GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 151.369 bits (381), Expect = 4.678e-40
Identity = 78/120 (65.00%), Postives = 83/120 (69.17%), Query Frame = 3 
Query:  129 MAKD--TGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            MAKD      E G +SAKDYTDPPP PL D  ELTKWS YRA+IAEFIA            IGYK Q+D   N     C+GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTF   L
Sbjct:    1 MAKDIEASAPEGGEFSAKDYTDPPPAPLIDVEELTKWSLYRAVIAEFIATLLFLYITVATVIGYKHQSDATVNTTDAACSGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL 120

HSP 2 Score: 28.1054 bits (61), Expect = 4.678e-40
Identity = 17/38 (44.74%), Postives = 22/38 (57.89%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKKRTGGGDNKL 616
            AV+Y++AQCL AI    +  GF       R GGG N+L
Sbjct:  129 AVLYIIAQCLGAICGVGLVKGFQSSY-YARYGGGANEL 165

HSP 3 Score: 27.7202 bits (60), Expect = 4.678e-40
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSL+R
Sbjct:  116 FGLFLARKVSLIR 128
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP23_MAIZE (Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 150.214 bits (378), Expect = 1.737e-39
Identity = 76/110 (69.09%), Postives = 80/110 (72.73%), Query Frame = 3 
Query:  153 EHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            E G +SAKDYTDPPP PL DA ELTKWS YRA+IAEFIA            IGYK QTD A +     C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTF   L
Sbjct:   12 EAGEFSAKDYTDPPPAPLIDADELTKWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDAAASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL 121

HSP 2 Score: 28.1054 bits (61), Expect = 1.737e-39
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSLVR
Sbjct:  117 FGLFLARKVSLVR 129

HSP 3 Score: 26.9498 bits (58), Expect = 1.737e-39
Identity = 16/38 (42.11%), Postives = 22/38 (57.89%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKKRTGGGDNKL 616
            A++Y++AQCL AI    +  GF       R GGG N+L
Sbjct:  130 ALLYIIAQCLGAICGVGLVKGFQSAY-YVRYGGGANEL 166
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP24_MAIZE (Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 149.828 bits (377), Expect = 2.258e-39
Identity = 78/120 (65.00%), Postives = 85/120 (70.83%), Query Frame = 3 
Query:  129 MAKDTGVA--EHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            MAKD   +  E G +SAKDYTDPPP PL DA ELT+WS YRA+IAEFIA            IGYK QTD + +     C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTF   L
Sbjct:    1 MAKDIEASGPEAGEFSAKDYTDPPPAPLIDAEELTQWSLYRAVIAEFIATLLFLYITVATVIGYKHQTDASASGPDAACGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL 120

HSP 2 Score: 28.1054 bits (61), Expect = 2.258e-39
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSLVR
Sbjct:  116 FGLFLARKVSLVR 128

HSP 3 Score: 26.9498 bits (58), Expect = 2.258e-39
Identity = 16/38 (42.11%), Postives = 22/38 (57.89%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKKRTGGGDNKL 616
            A++Y++AQCL AI    +  GF       R GGG N+L
Sbjct:  129 ALLYIIAQCLGAICGVGLVKGFQSAY-YVRYGGGANEL 165
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP24_ARATH (Probable aquaporin PIP2-4 OS=Arabidopsis thaliana GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 145.976 bits (367), Expect = 1.841e-38
Identity = 72/111 (64.86%), Postives = 77/111 (69.37%), Query Frame = 3 
Query:  129 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVT 461
            MAKD  V E G  +A+DY DPPP P FD  EL KW  YRA+IAEF+A            IGYK+QTD       C GVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVT
Sbjct:    1 MAKDLDVNESGPPAARDYKDPPPAPFFDMEELRKWPLYRAVIAEFVATLLFLYVSILTVIGYKAQTDATAGGVDCGGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVT 111

HSP 2 Score: 30.0314 bits (66), Expect = 1.841e-38
Identity = 19/40 (47.50%), Postives = 23/40 (57.50%), Query Frame = 2 
Query:  506 VMYMVAQCLCAIYRCWVGHGFPKILKKK---RTGGGDNKL 616
            V+Y+VAQCL AI  C    GF K  +     R GGG N+L
Sbjct:  126 VLYIVAQCLGAICGC----GFVKAFQSSYYTRYGGGANEL 161

HSP 3 Score: 25.7942 bits (55), Expect = 1.841e-38
Identity = 12/12 (100.00%), Postives = 12/12 (100.00%), Query Frame = 1 
Query:  466 GLFLARKVSLVR 501
            GLFLARKVSLVR
Sbjct:  113 GLFLARKVSLVR 124
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP22_ARATH (Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2 PE=1 SV=2)

HSP 1 Score: 142.51 bits (358), Expect = 1.842e-38
Identity = 72/116 (62.07%), Postives = 78/116 (67.24%), Query Frame = 3 
Query:  129 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            MAKD    E   +  +DY DPPP P FDA ELTKWS YRA+IAEF+A            IGYK Q+D       C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTF   L
Sbjct:    1 MAKDVEGPE--GFQTRDYEDPPPTPFFDADELTKWSLYRAVIAEFVATLLFLYITVLTVIGYKIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL 114

HSP 2 Score: 31.5722 bits (70), Expect = 1.842e-38
Identity = 21/41 (51.22%), Postives = 23/41 (56.10%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKK---RTGGGDNKL 616
            AV+YMVAQCL AI     G GF K  +     R GGG N L
Sbjct:  123 AVLYMVAQCLGAI----CGVGFVKAFQSSYYDRYGGGANSL 159

HSP 3 Score: 27.7202 bits (60), Expect = 1.842e-38
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSL+R
Sbjct:  110 FGLFLARKVSLIR 122
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP25_MAIZE (Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 144.05 bits (362), Expect = 6.836e-38
Identity = 73/118 (61.86%), Postives = 82/118 (69.49%), Query Frame = 3 
Query:  129 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVD--GCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            MAKD   A   ++  KDY+DPPP PL DA ELTKWS YRA+IAEF+A            IGYK QTD A +     C GVG+LGIAWAFGGMIF+LVYCTAG+SGGHINPAVTF   L
Sbjct:    1 MAKDIEAA--AAHEGKDYSDPPPAPLVDAEELTKWSLYRAVIAEFVATLLFLYITVATVIGYKHQTDAAASGPDAACGGVGVLGIAWAFGGMIFILVYCTAGVSGGHINPAVTFGLFL 116

HSP 2 Score: 28.1054 bits (61), Expect = 6.836e-38
Identity = 13/13 (100.00%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSLVR
Sbjct:  112 FGLFLARKVSLVR 124

HSP 3 Score: 27.7202 bits (60), Expect = 6.836e-38
Identity = 17/38 (44.74%), Postives = 22/38 (57.89%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKKRTGGGDNKL 616
            A++Y+VAQCL AI    +  GF       R GGG N+L
Sbjct:  125 ALLYIVAQCLGAICGVGLVKGFQSAF-YVRYGGGANEL 161
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP23_ARATH (Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.501e-37
Identity = 72/116 (62.07%), Postives = 78/116 (67.24%), Query Frame = 3 
Query:  129 MAKDTGVAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVDGCAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTIL 476
            MAKD  V     +  +DY DPPP P FDA ELTKWS YRA+IAEF+A            IGYK Q+D       C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTF   L
Sbjct:    1 MAKD--VEGPDGFQTRDYEDPPPTPFFDAEELTKWSLYRAVIAEFVATLLFLYVTVLTVIGYKIQSDTKAGGVDCGGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFL 114

HSP 2 Score: 28.4906 bits (62), Expect = 1.501e-37
Identity = 20/41 (48.78%), Postives = 22/41 (53.66%), Query Frame = 2 
Query:  503 AVMYMVAQCLCAIYRCWVGHGFPKILKKK---RTGGGDNKL 616
            AV+YMVAQCL AI     G GF K  +       GGG N L
Sbjct:  123 AVLYMVAQCLGAI----CGVGFVKAFQSSHYVNYGGGANFL 159

HSP 3 Score: 27.7202 bits (60), Expect = 1.501e-37
Identity = 12/13 (92.31%), Postives = 13/13 (100.00%), Query Frame = 1 
Query:  463 FGLFLARKVSLVR 501
            FGLFLARKVSL+R
Sbjct:  110 FGLFLARKVSLIR 122
[Close]
BLAST of EY753062 vs. ExPASy Swiss-Prot
Match: PIP27_MAIZE (Aquaporin PIP2-7 OS=Zea mays GN=PIP2-7 PE=2 SV=1)

HSP 1 Score: 150.984 bits (380), Expect = 9.329e-36
Identity = 93/170 (54.71%), Postives = 103/170 (60.59%), Query Frame = 3 
Query:  129 MAKDTG-VAEHGSYSAKDYTDPPPEPLFDAVELTKWSFYRALIAEFIAXXXXXXXXXXXXIGYKSQTDPAKNVDG---CAGVGILGIAWAFGGMIFVLVYCTAGISGGHINPAVTFRTILGPQGVVGPWL*CTWWRNAFVLSTGVGLVTAFQK-FLKKKGQEVGTTNLGH 623
            MAKD   V E G YSAKDY DPPP PL D  ELTKWS YRA IAEFIA            IGYK Q+D    + G   C GVGILGIAWAFGGMIF+LVYCTAGISGGHINPAVTF   LG +  +   L     + A  +  G GL   FQK F  + G  V T + G+
Sbjct:    1 MAKDVEQVTEQGEYSAKDYHDPPPAPLIDPDELTKWSLYRAAIAEFIATLLFLYITVLTIIGYKRQSDT--KIPGNTECDGVGILGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLGRKVSLVRALLYMIAQCAGAI-CGAGLAKGFQKSFYNRYGGGVNTVSDGY 167
The following BLAST results are available for this feature:
BLAST of EY753062 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 40
Match NameE-valueIdentityDescription
PIP25_ARATH2.307e-4372.73Probable aquaporin PIP2-5 OS=Arabidopsis thaliana ... [more]
PIP23_ORYSJ2.767e-4064.46Probable aquaporin PIP2-3 OS=Oryza sativa subsp. j... [more]
PIP22_ORYSJ4.678e-4065.00Probable aquaporin PIP2-2 OS=Oryza sativa subsp. j... [more]
PIP23_MAIZE1.737e-3969.09Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1[more]
PIP24_MAIZE2.258e-3965.00Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1[more]
PIP24_ARATH1.841e-3864.86Probable aquaporin PIP2-4 OS=Arabidopsis thaliana ... [more]
PIP22_ARATH1.842e-3862.07Aquaporin PIP2-2 OS=Arabidopsis thaliana GN=PIP2-2... [more]
PIP25_MAIZE6.836e-3861.86Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1[more]
PIP23_ARATH1.501e-3762.07Aquaporin PIP2-3 OS=Arabidopsis thaliana GN=PIP2-3... [more]
PIP27_MAIZE9.329e-3654.71Aquaporin PIP2-7 OS=Zea mays GN=PIP2-7 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C5-003-050-F02-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY753062 ID=EY753062; Name=EY753062; organism=Citrus sinensis; type=EST; length=942bp
CCCACGCGTCCGAAAAAAAAAGAAAAAGAAAAAGAAAAAGGAAAAGGCTT
CTCTCTCGTTGTGCTTGTGGCTTTGCAGTGCTCTCAGTTTTCTCTGTTTC
TCTCCAAATCATTGTTACTGGTTTTCCAATGGCGAAGGACACTGGGGTGG
CAGAGCACGGCTCATATTCAGCCAAGGACTACACGGACCCACCGCCGGAG
CCGCTATTCGACGCCGTGGAGCTGACCAAATGGTCATTCTACAGAGCCTT
GATAGCTGAGTTCATAGCAACCCTGCTCTTTCTTTACGTCACTGTCTTGA
CTGTCATTGGCTACAAAAGCCAGACCGATCCCGCCAAGAACGTCGACGGC
TGTGCCGGCGTCGGCATCCTCGGCATCGCCTGGGCCTTCGGTGGCATGAT
CTTTGTCCTCGTTTACTGCACCGCCGGTATCTCAGGTGGTCACATTAACC
CGGCAGTGACGTTTCGGACTATTCTTGGCCCGCAAGGTGTCGTTGGTCCG
TGGCTGTGATGTACATGGTGGCGCAATGCCTTTGTGCTATCTACCGGTGT
TGGGTTGGTCACGGCTTTCCAAAAATTCTTAAAAAAAAAAGGACAGGAGG
TGGGGACAACAAACTTGGGCCATGAAATCAACCTCCTCGTGTTGGAATGA
GCCTACTAATATATTTACAACCATTGCAATGGAATAAACCTGAAAATTGT
CAAAAAAAATATCCAATAAAAAAATCTAGAAAACTACCAAATAATACGAG
ATTAGGGCAACTACAGCCAAATCAATATAACCTGATATACAGAAACACAA
AATAAAAAAGAAGAAAGAAAACTAGGATATAGCAGAGTTGGAAAAATAAT
GAGAATAAAACCATTACAGAAATAATTAAAAAAATCAAAAAAGAAAAAAA
ATCATATATAAAAAAATAAAAATTACAAATATCTAATAGGAT
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