EY718763

Overview
NameEY718763
Unique NameEY718763
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length475
Libraries
Library NameType
Sweet orange fruit, development stadium (3 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_ARATH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Arabidopsis thaliana GN=CIMS PE=1 SV=1)

HSP 1 Score: 213.001 bits (541), Expect = 8.684e-57
Identity = 102/113 (90.27%), Postives = 108/113 (95.58%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            LSGFAFTANGWVQSYGSRCVKPP+IYGDVSRPKAMTVFWS++AQSMT+RPMKGMLTGPVTILN SFV+NDQPRHETCYQIALAIKDEVEDL+K GI VI IDEAALR GLPLR
Sbjct:  504 LSGFAFTANGWVQSYGSRCVKPPVIYGDVSRPKAMTVFWSAMAQSMTSRPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKDEVEDLEKGGIGVIQIDEAALREGLPLR 616

HSP 2 Score: 29.6462 bits (65), Expect = 8.684e-57
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  340 ESEHAFYLDWATTPFK 387
            +SEHAFYLDWA   F+
Sbjct:  617 KSEHAFYLDWAVHSFR 632
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_SOLSC (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Solenostemon scutellarioides GN=MET PE=1 SV=2)

HSP 1 Score: 207.608 bits (527), Expect = 3.557e-55
Identity = 102/113 (90.27%), Postives = 105/113 (92.92%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPK MTVFWS+ AQSMT RPMKGMLTGPVTILN SFV+NDQPR ETCYQIALAIKDEVEDL+KAGI VI IDEAALR GLPLR
Sbjct:  503 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKPMTVFWSTAAQSMTQRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGITVIQIDEAALREGLPLR 615

HSP 2 Score: 29.6462 bits (65), Expect = 3.557e-55
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  340 ESEHAFYLDWATTPFK 387
            +SEHAFYLDWA   F+
Sbjct:  616 KSEHAFYLDWAVHSFR 631
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_MESCR (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Mesembryanthemum crystallinum GN=METE PE=2 SV=1)

HSP 1 Score: 212.231 bits (539), Expect = 4.637e-55
Identity = 103/113 (91.15%), Postives = 108/113 (95.58%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            LSGFAF+ANGWVQSYGSRCVKPPIIYGDVSRP  MTVFWSS+AQSMTARPMKGMLTGPVTILN SFV+NDQPRHETCYQIALAIK+EVEDL+KAGINVI IDEAALR GLPLR
Sbjct:  504 LSGFAFSANGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSSMAQSMTARPMKGMLTGPVTILNWSFVRNDQPRHETCYQIALAIKNEVEDLEKAGINVIQIDEAALREGLPLR 616

HSP 2 Score: 24.6386 bits (52), Expect = 4.637e-55
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = 1 
Query:  340 ESEHAFYLDWATTPFK 387
            +SEH FYL WA   F+
Sbjct:  617 KSEHDFYLKWAVHSFR 632
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_CATRO (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Catharanthus roseus GN=METE PE=2 SV=1)

HSP 1 Score: 207.608 bits (527), Expect = 7.880e-55
Identity = 102/113 (90.27%), Postives = 104/113 (92.04%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRP  MTVFWS  AQSMT RPMKGMLTGPVTILN SFV+NDQPR ETCYQIALAIKDEVEDL+KAGINVI IDEAALR GLPLR
Sbjct:  504 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPNPMTVFWSQTAQSMTKRPMKGMLTGPVTILNWSFVRNDQPRFETCYQIALAIKDEVEDLEKAGINVIQIDEAALREGLPLR 616

HSP 2 Score: 28.4906 bits (62), Expect = 7.880e-55
Identity = 10/16 (62.50%), Postives = 13/16 (81.25%), Query Frame = 1 
Query:  340 ESEHAFYLDWATTPFK 387
            ++EHAFYLDWA   F+
Sbjct:  617 KAEHAFYLDWAVHSFR 632
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_BURCM (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain ATCC BAA-244 / AMMD) GN=metE PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.298e-44
Identity = 84/113 (74.34%), Postives = 99/113 (87.61%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            L G+AF+  GWVQSYGSRCVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILN SFV++DQPR  +CYQ+ALAI+DEV DL+KAG+ VI IDEAALR GLPLR
Sbjct:  507 LDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLR 619
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_BURA4 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia ambifaria (strain MC40-6) GN=metE PE=3 SV=1)

HSP 1 Score: 177.178 bits (448), Expect = 3.298e-44
Identity = 84/113 (74.34%), Postives = 99/113 (87.61%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            L G+AF+  GWVQSYGSRCVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILN SFV++DQPR  +CYQ+ALAI+DEV DL+KAG+ VI IDEAALR GLPLR
Sbjct:  507 LDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEKAGVRVIQIDEAALREGLPLR 619
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_FLAJ1 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Flavobacterium johnsoniae (strain ATCC 17061 / DSM 2064 / UW101) GN=metE PE=3 SV=1)

HSP 1 Score: 176.022 bits (445), Expect = 7.346e-44
Identity = 84/113 (74.34%), Postives = 95/113 (84.07%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            L GF FT NGWVQSYGSRCVKPP+IYGDVSRP  MTV WS  AQS+T++ +KGMLTGPVTIL  SFV+NDQPR ETC QIALA++DEV DL+KAGI +I IDE A+R GLPLR
Sbjct:  512 LDGFTFTKNGWVQSYGSRCVKPPVIYGDVSRPNPMTVKWSKYAQSLTSKWVKGMLTGPVTILQWSFVRNDQPRSETCTQIALAVRDEVVDLEKAGIKIIQIDEPAIREGLPLR 624
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_BURS3 (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia sp. (strain 383) GN=metE PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 9.594e-44
Identity = 83/113 (73.45%), Postives = 99/113 (87.61%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            L G+AF+  GWVQSYGSRCVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILN SFV++DQPR  +CYQ+ALAI++EV DL+KAG+ VI IDEAALR GLPLR
Sbjct:  507 LDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLR 619
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_BURCJ (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cepacia (strain J2315 / LMG 16656) GN=metE PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 9.594e-44
Identity = 83/113 (73.45%), Postives = 99/113 (87.61%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            L G+AF+  GWVQSYGSRCVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILN SFV++DQPR  +CYQ+ALAI+DEV DL++AG+ VI IDEAALR GLPLR
Sbjct:  507 LDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWITYAQSLTNKPMKGMLTGPVTILNWSFVRDDQPRSVSCYQLALAIRDEVLDLEQAGVRVIQIDEAALREGLPLR 619
[Close]
BLAST of EY718763 vs. ExPASy Swiss-Prot
Match: METE_BURCH (5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase OS=Burkholderia cenocepacia (strain HI2424) GN=metE PE=3 SV=1)

HSP 1 Score: 175.637 bits (444), Expect = 9.594e-44
Identity = 83/113 (73.45%), Postives = 99/113 (87.61%), Query Frame = 2 
Query:    2 LSGFAFTANGWVQSYGSRCVKPPIIYGDVSRPKAMTVFWSSLAQSMTARPMKGMLTGPVTILNLSFVKNDQPRHETCYQIALAIKDEVEDLDKAGINVIPIDEAALRGGLPLR 340
            L G+AF+  GWVQSYGSRCVKPPI++GD+SRPKAMTV W + AQS+T +PMKGMLTGPVTILN SFV++DQPR  +CYQ+ALAI++EV DL+KAG+ VI IDEAALR GLPLR
Sbjct:  507 LDGYAFSQFGWVQSYGSRCVKPPILFGDISRPKAMTVEWIAYAQSLTRKPMKGMLTGPVTILNWSFVRDDQPRAVSCYQLALAIREEVLDLEKAGVRVIQIDEAALREGLPLR 619
The following BLAST results are available for this feature:
BLAST of EY718763 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 269
Match NameE-valueIdentityDescription
METE_ARATH8.684e-5790.275-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_SOLSC3.557e-5590.275-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_MESCR4.637e-5591.155-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_CATRO7.880e-5590.275-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCM3.298e-4474.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURA43.298e-4474.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_FLAJ17.346e-4474.345-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURS39.594e-4473.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCJ9.594e-4473.455-methyltetrahydropteroyltriglutamate--homocystein... [more]
METE_BURCH9.594e-4473.455-methyltetrahydropteroyltriglutamate--homocystein... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-702-094-A11-CT.F Sweet orange fruit, development stadium (3 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY718763 ID=EY718763; Name=EY718763; organism=Citrus sinensis; type=EST; length=475bp
ATTATCAGGTTTCGCCTTCACTGCCAATGGGTGGGTGCAATCCTATGGAT
CTCGCTGTGTGAAGCCACCCATCATCTATGGTGATGTGAGCCGTCCCAAG
GCTATGACCGTCTTCTGGTCCTCCTTGGCTCAAAGCATGACAGCTCGCCC
AATGAAAGGAATGCTTACTGGCCCTGTCACCATTCTCAACTTGTCCTTTG
TTAAAAATGACCAGCCCAGACATGAAACCTGCTACCAAATTGCTTTGGCC
ATCAAGGACGAAGTCGAAGATCTTGACAAAGCAGGCATTAACGTTATTCC
AATTGATGAAGCTGCCTTGAGGGGAGGATTGCCTCTTAGGAATCTGAGCA
TGCTTTCTACTTGGACTGGGCCACCACTCCTTTCAAATCCCCACTGGGGC
CTCCAGGAACCCTACCCGACCGCACCCCATGTGCTCTTCCACTTCAACAA
CTTGACCGCTCCATATCCACAATGA
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