EY693128 | Citrus Genome Database

Overview


Name	EY693128
Unique Name	EY693128
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	875

Libraries


Library Name	Type
Sweet orange fruit, development stadium (1 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: CCD1_ARATH (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Arabidopsis thaliana GN=CCD1 PE=1 SV=2)

HSP 1 Score: 203.756 bits (517), Expect = 4.795e-66
Identity = 93/112 (83.04%), Postives = 102/112 (91.07%), Query Frame = 2

Query:  113 TDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            T EMFTFGY+ TPPY+TYRVISKDG MHDPVPIT+S PIMMHDFAITE YAIF+DLPM FR KEMVKE K+IY+FDPT KARFGVLPRYA+D+L IRWFELPNC+IFHNANA
Sbjct:  229 TGEMFTFGYSHTPPYLTYRVISKDGIMHDPVPITISEPIMMHDFAITETYAIFMDLPMHFRPKEMVKEKKMIYSFDPTKKARFGVLPRYAKDELMIRWFELPNCFIFHNANA 340

HSP 2 Score: 69.707 bits (169), Expect = 4.795e-66
Identity = 45/115 (39.13%), Postives = 61/115 (53.04%), Query Frame = 1

Query:  478 VLITCRVRNPDLDMFNGPLKETLEDQTTELYEMRFNMKTGLASQKSCHQRVLIFLG*TSTTWAGNRDMFM-GAFWET*XRSRGLSNLICMLNRRRGKQSLS-WGEC*GIFHWGPG 816
            VLITCR+ NPDLDM +G +KE LE+   ELYEMRFNMKTG ASQK      + F    +  + G +  ++ G   ++  +  G+           GK+ L   G   GI+  G G
Sbjct:  348 VLITCRLENPDLDMVSGKVKEKLENFGNELYEMRFNMKTGSASQKKLSASAVDFPR-INECYTGKKQRYVYGTILDSIAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEG 461

HSP 3 Score: 97.8265 bits (242), Expect = 8.404e-20
Identity = 48/73 (65.75%), Postives = 59/73 (80.82%), Query Frame = 3

Query:  609 KKLSPACVDFPRINEHYMGRKQRYVYGSILGDIAKVTGLIKFDLHAEPETGKTKLKLGGMLRHL-PLGPGKFG 824
            KKLS + VDFPRINE Y G+KQRYVYG+IL  IAKVTG+IKFDLHAE ETGK  L++GG ++ +  LG G++G
Sbjct:  392 KKLSASAVDFPRINECYTGKKQRYVYGTILDSIAKVTGIIKFDLHAEAETGKRMLEVGGNIKGIYDLGEGRYG 464

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: CCD_CROSA (Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Crocus sativus GN=CCD PE=1 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 1.970e-64
Identity = 88/112 (78.57%), Postives = 98/112 (87.50%), Query Frame = 2

Query:  113 TDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            TDEMFTFGY  TPPYVTYRVISKDG M DPVPIT+ A +MMHDFAITENY+IF+DLP+ F+ KEMVK  KLI++FD T KARFGVLPRYA+DD  IRWFELPNC+IFHNANA
Sbjct:  233 TDEMFTFGYAHTPPYVTYRVISKDGVMRDPVPITIPASVMMHDFAITENYSIFMDLPLYFQPKEMVKGGKLIFSFDATKKARFGVLPRYAKDDSLIRWFELPNCFIFHNANA 344

HSP 2 Score: 77.0258 bits (188), Expect = 1.970e-64
Identity = 47/114 (41.23%), Postives = 58/114 (50.88%), Query Frame = 1

Query:  478 VLITCRVRNPDLDMFNGPLKETLEDQTTELYEMRFNMKTGLASQKSCHQRVLIFLG*TSTTWAGNRDMFMGAFWET*XRSRGLSNLICMLNRRRGKQSLS-WGEC*GIFHWGPG 816
            VLITCR+ NPDLDM NG +KE LE+   ELYEMRFNMKTG ASQK      + F     +     +    G   +   + +G+           GK+ L   G   GIF  GPG
Sbjct:  352 VLITCRLENPDLDMVNGAVKEKLENFKNELYEMRFNMKTGAASQKQLSVSAVDFPRINESYTTRKQRYVYGTILDNITKVKGIIKFDLHAEPEAGKKKLEVGGNVQGIFDLGPG 465

HSP 3 Score: 97.0561 bits (240), Expect = 1.433e-19
Identity = 47/73 (64.38%), Postives = 59/73 (80.82%), Query Frame = 3

Query:  609 KKLSPACVDFPRINEHYMGRKQRYVYGSILGDIAKVTGLIKFDLHAEPETGKTKLKLGGMLRHL-PLGPGKFG 824
            K+LS + VDFPRINE Y  RKQRYVYG+IL +I KV G+IKFDLHAEPE GK KL++GG ++ +  LGPG++G
Sbjct:  396 KQLSVSAVDFPRINESYTTRKQRYVYGTILDNITKVKGIIKFDLHAEPEAGKKKLEVGGNVQGIFDLGPGRYG 468

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: CCD1_PHAVU (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Phaseolus vulgaris GN=CCD1 PE=1 SV=1)

HSP 1 Score: 194.512 bits (493), Expect = 3.977e-62
Identity = 87/112 (77.68%), Postives = 99/112 (88.39%), Query Frame = 2

Query:  113 TDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            T EMF+FGY  TPPY+TYRVISKDG+MHDPVPIT+S PIMMHDFAITENYA+F+DLP+ FR KEMVK   LI++FD T KARFGVLPRYA+D+  IRWFELPNC+IFHNANA
Sbjct:  231 TGEMFSFGYAHTPPYITYRVISKDGYMHDPVPITISDPIMMHDFAITENYAVFMDLPLIFRPKEMVKNKTLIFSFDSTKKARFGVLPRYAKDEQHIRWFELPNCFIFHNANA 342

HSP 2 Score: 65.855 bits (159), Expect = 3.977e-62
Identity = 42/115 (36.52%), Postives = 61/115 (53.04%), Query Frame = 1

Query:  478 VLITCRVRNPDLDMFNGPLKETLEDQTTELYEMRFNMKTGLASQKSCHQRVLIFLG*TSTTWAGNRDMFM-GAFWET*XRSRGLSNLICMLNRRRGKQSLS-WGEC*GIFHWGPG 816
            VLITCR++NP LD   G ++E LE+ + ELYEMRFNMKTG ASQK      + F    +  + G +  ++ G   ++  +  G+           GK+ L   G   G++  GPG
Sbjct:  350 VLITCRLQNPKLDNVGGTVQEKLENFSNELYEMRFNMKTGEASQKKLSASTVDFPR-VNENYTGRKQRYVYGTTLDSIAKVTGIIKFDLHAEPDHGKEKLEVGGNVQGLYDLGPG 463

HSP 3 Score: 104.76 bits (260), Expect = 6.875e-22
Identity = 51/73 (69.86%), Postives = 61/73 (83.56%), Query Frame = 3

Query:  609 KKLSPACVDFPRINEHYMGRKQRYVYGSILGDIAKVTGLIKFDLHAEPETGKTKLKLGGMLRHL-PLGPGKFG 824
            KKLS + VDFPR+NE+Y GRKQRYVYG+ L  IAKVTG+IKFDLHAEP+ GK KL++GG ++ L  LGPGKFG
Sbjct:  394 KKLSASTVDFPRVNENYTGRKQRYVYGTTLDSIAKVTGIIKFDLHAEPDHGKEKLEVGGNVQGLYDLGPGKFG 466

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: CCD1_PEA (Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=Pisum sativum GN=CCD1 PE=2 SV=1)

HSP 1 Score: 191.815 bits (486), Expect = 1.366e-61
Identity = 88/112 (78.57%), Postives = 98/112 (87.50%), Query Frame = 2

Query:  113 TDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            T EMFTFGY+ T PYVTYRVISKDGFM DPVPIT+S P+MMHDFAITENY+IF+DLP+ FR KEMVK   LI++FD T KARFGVLPRYA+DD  IRWFELPNC+IFHNANA
Sbjct:  229 TGEMFTFGYSHTAPYVTYRVISKDGFMQDPVPITISDPVMMHDFAITENYSIFMDLPLYFRPKEMVKNKTLIFSFDSTKKARFGVLPRYAKDDKHIRWFELPNCFIFHNANA 340

HSP 2 Score: 66.6254 bits (161), Expect = 1.366e-61
Identity = 42/115 (36.52%), Postives = 62/115 (53.91%), Query Frame = 1

Query:  478 VLITCRVRNPDLDMFNGPLKETLEDQTTELYEMRFNMKTGLASQKSCHQRVLIFLG*TSTTWAGNRDMFM-GAFWET*XRSRGLSNLICMLNRRRGKQSLS-WGEC*GIFHWGPG 816
            VLITCR+ NP+LD+  G +KE L++ + ELYEMRFNMKTG ASQK      + F    + ++ G +  ++ G   ++  +  G+           GK  L   G   G++  GPG
Sbjct:  348 VLITCRLENPNLDVVGGAVKEKLDNFSNELYEMRFNMKTGEASQKKLSASTVDFPR-VNESYTGRKQRYVYGTTLDSIAKVTGIIKFDLHAEPDSGKTKLEVGGNVQGLYDLGPG 461

HSP 3 Score: 21.1718 bits (43), Expect = 1.366e-61
Identity = 6/9 (66.67%), Postives = 9/9 (100.00%), Query Frame = 1

Query:   13 PKIDPYTGK 39
            PK+DP+TG+
Sbjct:  223 PKVDPFTGE 231

HSP 4 Score: 106.301 bits (264), Expect = 2.363e-22
Identity = 51/73 (69.86%), Postives = 62/73 (84.93%), Query Frame = 3

Query:  609 KKLSPACVDFPRINEHYMGRKQRYVYGSILGDIAKVTGLIKFDLHAEPETGKTKLKLGGMLRHL-PLGPGKFG 824
            KKLS + VDFPR+NE Y GRKQRYVYG+ L  IAKVTG+IKFDLHAEP++GKTKL++GG ++ L  LGPG+FG
Sbjct:  392 KKLSASTVDFPRVNESYTGRKQRYVYGTTLDSIAKVTGIIKFDLHAEPDSGKTKLEVGGNVQGLYDLGPGRFG 464

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: NCED3_ARATH (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana GN=NCED3 PE=2 SV=1)

HSP 1 Score: 85.5001 bits (210), Expect = 4.316e-16
Identity = 43/111 (38.74%), Postives = 61/111 (54.95%), Query Frame = 2

Query:  119 EMFTFGY-TQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            E+F   Y   + PY+ Y   S DG     V I +  P MMHDFAITEN+ +  D  + F+  EM++    +  +D    ARFG+L +YA D   I+W + P+C+ FH  NA
Sbjct:  306 ELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDFAITENFVVVPDQQVVFKLPEMIRGGSPV-VYDKNKVARFGILDKYAEDSSNIKWIDAPDCFCFHLWNA 415

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: ZCD_CROSA (Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromoplast OS=Crocus sativus GN=ZCD PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.142e-15
Identity = 47/114 (41.23%), Postives = 65/114 (57.02%), Query Frame = 2

Query:  113 TDEMFTFGYTQTPPYVTYRVISKDGFMHDP-VPI-TMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            T E F+F Y    PYVTY     DG    P VPI +   P  +HDFAITE+YA+F D+ +  +  E+V+  ++I   D     R G+LPRYA  D ++RWF++P   + H  NA
Sbjct:   69 TGETFSFSYGPIQPYVTYSRYDCDGKKSGPDVPIFSFKEPSFVHDFAITEHYAVFPDIQIVMKPAEIVRGRRMIGP-DLEKVPRLGLLPRYATSDSEMRWFDVPGFNMVHVVNA 181

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: NCED9_ARATH (9-cis-epoxycarotenoid dioxygenase NCED9, chloroplastic OS=Arabidopsis thaliana GN=NCED9 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.142e-15
Identity = 42/113 (37.17%), Postives = 64/113 (56.64%), Query Frame = 2

Query:  113 TDEMFTFGY-TQTPPYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            T E+F   Y   + PY+ Y   + DG     V I +  P M+HDFAITEN+ +  D  + FR  EM++    +  +D   K+RFG+L + A+D   I+W E+P+C+ FH  N+
Sbjct:  364 TRELFALSYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMIRGGSPV-VYDEKKKSRFGILNKNAKDASSIQWIEVPDCFCFHLWNS 475

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: NCED1_MAIZE (9-cis-epoxycarotenoid dioxygenase 1, chloroplastic OS=Zea mays GN=VP14 PE=1 SV=2)

HSP 1 Score: 78.1814 bits (191), Expect = 1.275e-14
Identity = 38/99 (38.38%), Postives = 57/99 (57.58%), Query Frame = 2

Query:  152 PYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            PY+ Y     DG   D V I +  P M+HDFAITEN  +  D  + F+ +EM++    +   D    +RFGVLP++A D  ++ W ++P+C+ FH  NA
Sbjct:  319 PYLKYFYFRPDGTKSDDVEIPLEQPTMIHDFAITENLVVVPDHQVVFKLQEMLRGGSPV-VLDKEKTSRFGVLPKHAADASEMAWVDVPDCFCFHLWNA 416

HSP 2 Score: 22.7126 bits (47), Expect = 1.275e-14
Identity = 8/13 (61.54%), Postives = 10/13 (76.92%), Query Frame = 1

Query:   13 PKIDPYTGKTHIL 51
            PK+DP TG+ H L
Sbjct:  299 PKLDPATGELHAL 311

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: CCD4_ARATH (Probable carotenoid cleavage dioxygenase 4, chloroplastic OS=Arabidopsis thaliana GN=CCD4 PE=1 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 4.040e-14
Identity = 43/115 (37.39%), Postives = 63/115 (54.78%), Query Frame = 2

Query:  113 TDEMFTFGYTQTPPYVTYRVISKDGFMHDPVPI-TMSAPIMMHDFAITENYAIFLDLPMCFRQK--EMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            T E F F Y   PP++TY      G     VPI +M++P  +HDFAIT+ +AIF ++ +  R    ++V E       D     R GV+P+YA D+ +++WFE+P   I H  NA
Sbjct:  294 TGETFAFRYGPVPPFLTYFRFDSAGKKQRDVPIFSMTSPSFLHDFAITKRHAIFAEIQLGMRMNMLDLVLEGGSPVGTDNGKTPRLGVIPKYAGDESEMKWFEVPGFNIIHAINA 408

[Close]

BLAST of EY693128 vs. ExPASy Swiss-Prot
Match: NCED1_PHAVU (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris GN=NCED1 PE=2 SV=1)

HSP 1 Score: 77.0258 bits (188), Expect = 1.343e-13
Identity = 38/99 (38.38%), Postives = 54/99 (54.55%), Query Frame = 2

Query:  152 PYVTYRVISKDGFMHDPVPITMSAPIMMHDFAITENYAIFLDLPMCFRQKEMVKENKLIYAFDPTIKARFGVLPRYARDDLQIRWFELPNCYIFHNANA 448
            PY+ Y   S DG     V I +  P MMHDFAITEN+ +  D  + F+  EM+     +  +D    +RFG+L + A+D   +RW + P C+ FH  NA
Sbjct:  337 PYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFVVVPDQQVVFKLTEMITGGSPV-VYDKNKTSRFGILDKNAKDANAMRWIDAPECFCFHLWNA 434

HSP 2 Score: 20.4014 bits (41), Expect = 1.343e-13
Identity = 7/13 (53.85%), Postives = 8/13 (61.54%), Query Frame = 1

Query:   13 PKIDPYTGKTHIL 51
            PK+DP  G  H L
Sbjct:  317 PKLDPVNGDLHAL 329

The following BLAST results are available for this feature:

BLAST of EY693128 vs. ExPASy Swiss-Prot

BLAST of EY693128 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13


Match Name	E-value	Identity	Description
CCD1_ARATH	4.795e-66	83.04	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=...	[more]
CCD_CROSA	1.970e-64	78.57	Carotenoid 9,10(9',10')-cleavage dioxygenase OS=Cr...	[more]
CCD1_PHAVU	3.977e-62	77.68	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=...	[more]
CCD1_PEA	1.366e-61	78.57	Carotenoid 9,10(9',10')-cleavage dioxygenase 1 OS=...	[more]
NCED3_ARATH	4.316e-16	38.74	9-cis-epoxycarotenoid dioxygenase NCED3, chloropla...	[more]
ZCD_CROSA	2.142e-15	41.23	Zeaxanthin 7,8(7',8')-cleavage dioxygenase, chromo...	[more]
NCED9_ARATH	2.142e-15	37.17	9-cis-epoxycarotenoid dioxygenase NCED9, chloropla...	[more]
NCED1_MAIZE	1.275e-14	38.38	9-cis-epoxycarotenoid dioxygenase 1, chloroplastic...	[more]
CCD4_ARATH	4.040e-14	37.39	Probable carotenoid cleavage dioxygenase 4, chloro...	[more]
NCED1_PHAVU	1.343e-13	38.38	9-cis-epoxycarotenoid dioxygenase NCED1, chloropla...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C3-700-008-B03-CT.F Sweet orange fruit, development stadium (1 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY693128 ID=EY693128; Name=EY693128; organism=Citrus sinensis; type=EST; length=875bp
CCACGCGTCCGTCCAAAGATTGACCCTTACACTGGTAAAACCCATATCCT
TAAAATAATCCTGTCAGCCAAATTTTCCCACAGAAAAAGAATGATTCACA
AGATATCTGCATACAGACGAGATGTTTACATTTGGTTATACACAAACTCC
TCCATATGTCACCTACCGAGTTATATCGAAGGATGGTTTCATGCATGACC
CAGTACCTATAACAATGTCTGCCCCCATCATGATGCACGACTTTGCCATC
ACTGAGAATTATGCAATTTTCCTGGATCTTCCCATGTGTTTCAGACAAAA
GGAGATGGTAAAAGAAAACAAGTTGATATATGCATTTGATCCAACTATAA
AAGCTCGTTTTGGTGTACTTCCACGATATGCGAGGGATGATCTCCAAATC
AGATGGTTTGAGCTTCCAAATTGCTATATATTCCATAATGCCAATGCNTT
GGGAGGAGGAGGNAGGAGGAGGAANTTGTTTTGATAACTTGCCGCGTACG
GAATCCCGATCTAGATATGTTTAATGGGCCTCTTAAAGAAACTCTTGAAG
ATCAGACGACTGAGCTATATGAGATGCGGTTCAATATGAAAACTGGCCTA
GCTTCTCAAAAAAGCTGTCACCAGCGTGTGTTGATTTTCCTAGGATAAAC
GAGCACTACATGGGCAGGAAACAGAGATATGTTTATGGGAGCATTTTGGG
AGACATAGCNAAGGTCACGGGGCTTATCAAATTTGATCTGCATGCTGAAC
CGGAGACGGGGAAAACAAAGCTTAAGTTGGGGGGAATGTTAAGGCATCTT
CCACTGGGGCCCGGGAAATTTGGTCAAAGGCTGGGTTGTTCCACAAAGCC
TATTCCCTTCTGAAAAGAGACGGTA