EY691939

Overview
NameEY691939
Unique NameEY691939
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length912
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_MAIZE (Soluble inorganic pyrophosphatase OS=Zea mays GN=IPP PE=2 SV=1)

HSP 1 Score: 133.265 bits (334), Expect = 2.742e-48
Identity = 65/89 (73.03%), Postives = 70/89 (78.65%), Query Frame = 3 
Query:  210 ETSSKTPVSQPPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            E  +     QP   P LNERILSS+SRRSVAAHPWHDLEIGP AP +FN V+EI KGSKVKYELDKK+GLI VDRVLYSSVVYPH   F
Sbjct:    4 EDKTAASAEQPKRAPKLNERILSSLSRRSVAAHPWHDLEIGPDAPAVFNVVVEITKGSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGF 92

HSP 2 Score: 80.8777 bits (198), Expect = 2.742e-48
Identity = 45/91 (49.45%), Postives = 56/91 (61.54%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFE 732
            P NYGF+PRTLCEDNDP+DVL++MQEPV+PG FL   R       IDQGE+    D ++A   + P   HY+      PH    +I RFFE
Sbjct:   87 PHNYGFVPRTLCEDNDPMDVLVLMQEPVVPGSFLRA-RAIGLMPMIDQGEKD---DKIIAVCADDPEYRHYNDISELSPHRL-QEIKRFFE 172
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_SOLTU (Soluble inorganic pyrophosphatase OS=Solanum tuberosum GN=PPA PE=2 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 2.977e-47
Identity = 61/75 (81.33%), Postives = 65/75 (86.67%), Query Frame = 3 
Query:  252 PPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            P LNERILSS+SRRSVAAHPWHDLEIGP AP +FN VIEI KGSKVKYELDKK+GLI VDR+LYSSVVYP    F
Sbjct:   15 PRLNERILSSISRRSVAAHPWHDLEIGPEAPSVFNVVIEISKGSKVKYELDKKTGLIKVDRILYSSVVYPQNYGF 89

HSP 2 Score: 84.7297 bits (208), Expect = 2.977e-47
Identity = 48/91 (52.75%), Postives = 56/91 (61.54%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFE 732
            P NYGFIPRTLCEDNDP+DVL++MQEPVLPGCFL   R       IDQGE+    D ++A   + P   HY       PH    +I RFFE
Sbjct:   84 PQNYGFIPRTLCEDNDPMDVLVLMQEPVLPGCFLRA-RAIGLMPMIDQGEKD---DKIIAVCADDPEYRHYTDIKQLPPHRL-AEIRRFFE 169
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_HORVD (Soluble inorganic pyrophosphatase OS=Hordeum vulgare var. distichum GN=IPP PE=2 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 3.229e-46
Identity = 63/87 (72.41%), Postives = 71/87 (81.61%), Query Frame = 3 
Query:  216 SSKTPVSQPPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            +++ P S+P   P LNERILSS+SRR   AHPWHDLEIGPGAP +FN V+EI KGSKVKYELDKK+GLI VDRVLYSSVVYPH   F
Sbjct:   11 AAQQPTSRPA--PKLNERILSSLSRRGGGAHPWHDLEIGPGAPAVFNVVVEITKGSKVKYELDKKTGLIKVDRVLYSSVVYPHNYGF 95

HSP 2 Score: 78.5666 bits (192), Expect = 3.229e-46
Identity = 42/85 (49.41%), Postives = 51/85 (60.00%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVK 714
            P NYGFIPRTLCEDNDP+DVL++MQEPV+PG FL   R       IDQGE+    D ++A   + P   HY       P L  +K
Sbjct:   90 PHNYGFIPRTLCEDNDPMDVLVLMQEPVIPGSFLRA-RAIGLMPMIDQGEKD---DKIIAVCADDPEYRHYSTSVSLLPRLQEIK 170
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_ORYSJ (Soluble inorganic pyrophosphatase OS=Oryza sativa subsp. japonica GN=IPP PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 4.209e-46
Identity = 65/91 (71.43%), Postives = 72/91 (79.12%), Query Frame = 3 
Query:  210 ETSSKTPVSQ--PPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            E   K P+    PPA   LNERILSSMS++ VAAHPWHDLEIGPGAP +FNCV+EI +GSKVKYELDK +GLI VDRVLYSSVVYPH   F
Sbjct:    4 EADGKAPLGSRYPPA--ALNERILSSMSQKHVAAHPWHDLEIGPGAPAVFNCVVEIPRGSKVKYELDKATGLIKVDRVLYSSVVYPHNYGF 92

HSP 2 Score: 77.0258 bits (188), Expect = 4.209e-46
Identity = 49/107 (45.79%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFEXXXXXXXXXXVAVNGF 780
            P NYGFIPRTLCED DP+DVL++MQE V+PGCFL   R       IDQGE+    D ++A   + P   H+       PH    +I RFFE          VAVN F
Sbjct:   87 PHNYGFIPRTLCEDGDPMDVLVLMQEQVVPGCFLRA-RAIGLMPMIDQGEKD---DKIIAVCADDPEYRHFRDIKEIPPHRL-QEIRRFFE-DYKKNENKEVAVNEF 187
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_ORYSI (Soluble inorganic pyrophosphatase OS=Oryza sativa subsp. indica GN=IPP PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 4.209e-46
Identity = 65/91 (71.43%), Postives = 72/91 (79.12%), Query Frame = 3 
Query:  210 ETSSKTPVSQ--PPAHPPLNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            E   K P+    PPA   LNERILSSMS++ VAAHPWHDLEIGPGAP +FNCV+EI +GSKVKYELDK +GLI VDRVLYSSVVYPH   F
Sbjct:    4 EADGKAPLGSRYPPA--ALNERILSSMSQKHVAAHPWHDLEIGPGAPAVFNCVVEIPRGSKVKYELDKATGLIKVDRVLYSSVVYPHNYGF 92

HSP 2 Score: 77.0258 bits (188), Expect = 4.209e-46
Identity = 49/107 (45.79%), Postives = 59/107 (55.14%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFEXXXXXXXXXXVAVNGF 780
            P NYGFIPRTLCED DP+DVL++MQE V+PGCFL   R       IDQGE+    D ++A   + P   H+       PH    +I RFFE          VAVN F
Sbjct:   87 PHNYGFIPRTLCEDGDPMDVLVLMQEQVVPGCFLRA-RAIGLMPMIDQGEKD---DKIIAVCADDPEYRHFRDIKEIPPHRL-QEIRRFFE-DYKKNENKEVAVNEF 187
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR2_ARATH (Soluble inorganic pyrophosphatase 2 OS=Arabidopsis thaliana GN=PPA2 PE=2 SV=2)

HSP 1 Score: 116.316 bits (290), Expect = 2.440e-25
Identity = 53/73 (72.60%), Postives = 59/73 (80.82%), Query Frame = 3 
Query:  258 LNERILSSMSRRSVAAHPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            LNER  ++ + RS AAHPWHDLEIGP AP +FNCV+EI KG KVKYELDK SGLI VDRVLYSS+VYPH   F
Sbjct:   24 LNERNFAAFTHRSAAAHPWHDLEIGPEAPTVFNCVVEISKGGKVKYELDKNSGLIKVDRVLYSSIVYPHNYGF 96

HSP 2 Score: 77.0258 bits (188), Expect = 1.649e-13
Identity = 49/123 (39.84%), Postives = 65/123 (52.85%), Query Frame = 1 
Query:  364 LKLGKGAR*NMNLTKNLD*SWLIVFFTLQLCTPINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFE 732
            +++ KG +    L KN     +       +  P NYGFIPRT+CED+DP+DVL++MQEPVL G FL   R       IDQGE+    D ++A   + P   HY       PH    +I RFFE
Sbjct:   59 VEISKGGKVKYELDKNSGLIKVDRVLYSSIVYPHNYGFIPRTICEDSDPMDVLVLMQEPVLTGSFLRA-RAIGLMPMIDQGEKD---DKIIAVCADDPEFRHYRDIKELPPHRL-AEIRRFFE 176
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_LEPIN (Inorganic pyrophosphatase OS=Leptospira interrogans GN=ppa PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 9.546e-21
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 3 
Query:  306 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            HPWHD+  G   P+I N VIEI +GS+ KYE+DK+ G++ +DRVLYSS  YP    F
Sbjct:    3 HPWHDISPGDQNPEIVNGVIEIKRGSRAKYEVDKEYGILKLDRVLYSSFYYPANYGF 59

HSP 2 Score: 52.373 bits (124), Expect = 9.546e-21
Identity = 32/91 (35.16%), Postives = 47/91 (51.65%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFE 732
            P NYGFIP++ C D DPLD+L++ Q  + P C +   +       +D GEE    D ++A A    S  H +      PH F +++  FFE
Sbjct:   54 PANYGFIPQSYCGDQDPLDILVLSQVELEPLCLVKA-KVIGVMRMLDSGEED---DKIIAVAANDMSVNHINDISELPPH-FTLELKHFFE 139
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_LEPIC (Inorganic pyrophosphatase OS=Leptospira interrogans serogroup Icterohaemorrhagiae serovar copenhageni GN=ppa PE=3 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 9.546e-21
Identity = 31/57 (54.39%), Postives = 40/57 (70.18%), Query Frame = 3 
Query:  306 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYPHKLWF 476
            HPWHD+  G   P+I N VIEI +GS+ KYE+DK+ G++ +DRVLYSS  YP    F
Sbjct:    3 HPWHDISPGDQNPEIVNGVIEIKRGSRAKYEVDKEYGILKLDRVLYSSFYYPANYGF 59

HSP 2 Score: 52.373 bits (124), Expect = 9.546e-21
Identity = 32/91 (35.16%), Postives = 47/91 (51.65%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEEKNG*DHLVAGAEESPSTEHYHGP*GAFPHLFGVKIPRFFE 732
            P NYGFIP++ C D DPLD+L++ Q  + P C +   +       +D GEE    D ++A A    S  H +      PH F +++  FFE
Sbjct:   54 PANYGFIPQSYCGDQDPLDILVLSQVELEPLCLVKA-KVIGVMRMLDSGEED---DKIIAVAANDMSVNHINDISELPPH-FTLELKHFFE 139
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_PYRFU (Inorganic pyrophosphatase OS=Pyrococcus furiosus GN=ppa PE=1 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 7.826e-20
Identity = 31/52 (59.62%), Postives = 40/52 (76.92%), Query Frame = 3 
Query:  306 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            +P+HDLE GP  P++   +IEI KGS+ KYELDKK+GL+ +DRVLYS   YP
Sbjct:    2 NPFHDLEPGPDVPEVVYAIIEIPKGSRNKYELDKKTGLLKLDRVLYSPFFYP 53

HSP 2 Score: 49.2914 bits (116), Expect = 7.826e-20
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEE 612
            P++YG IPRT  ED+DP D+++IM+EPV P   +   RP      ID G++
Sbjct:   53 PVDYGIIPRTWYEDDDPFDIMVIMREPVYPLTIIEA-RPIGLFKMIDSGDK 102
[Close]
BLAST of EY691939 vs. ExPASy Swiss-Prot
Match: IPYR_THELI (Inorganic pyrophosphatase OS=Thermococcus litoralis GN=ppa PE=3 SV=1)

HSP 1 Score: 68.1662 bits (165), Expect = 2.240e-19
Identity = 32/52 (61.54%), Postives = 40/52 (76.92%), Query Frame = 3 
Query:  306 HPWHDLEIGPGAPKIFNCVIEIGKGSKVKYELDKKSGLIMVDRVLYSSVVYP 461
            +P+HDLE GP  P++   +IEI KGS+ KYELDKK+GLI +DRVLYS   YP
Sbjct:    2 NPFHDLEPGPEVPEVVYALIEIPKGSRNKYELDKKTGLIKLDRVLYSPFHYP 53

HSP 2 Score: 48.9062 bits (115), Expect = 2.240e-19
Identity = 21/51 (41.18%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  460 PINYGFIPRTLCEDNDPLDVLIIMQEPVLPGCFLSG*RPSEFCL*IDQGEE 612
            P++YG IP+T  +D+DP D+++IM+EP  PG  +   RP      ID G++
Sbjct:   53 PVDYGIIPQTWYDDDDPFDIMVIMREPTYPGVLIEA-RPIGLFKMIDSGDK 102
The following BLAST results are available for this feature:
BLAST of EY691939 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42
Match NameE-valueIdentityDescription
IPYR_MAIZE2.742e-4873.03Soluble inorganic pyrophosphatase OS=Zea mays GN=I... [more]
IPYR_SOLTU2.977e-4781.33Soluble inorganic pyrophosphatase OS=Solanum tuber... [more]
IPYR_HORVD3.229e-4672.41Soluble inorganic pyrophosphatase OS=Hordeum vulga... [more]
IPYR_ORYSJ4.209e-4671.43Soluble inorganic pyrophosphatase OS=Oryza sativa ... [more]
IPYR_ORYSI4.209e-4671.43Soluble inorganic pyrophosphatase OS=Oryza sativa ... [more]
IPYR2_ARATH2.440e-2572.60Soluble inorganic pyrophosphatase 2 OS=Arabidopsis... [more]
IPYR_LEPIN9.546e-2154.39Inorganic pyrophosphatase OS=Leptospira interrogan... [more]
IPYR_LEPIC9.546e-2154.39Inorganic pyrophosphatase OS=Leptospira interrogan... [more]
IPYR_PYRFU7.826e-2059.62Inorganic pyrophosphatase OS=Pyrococcus furiosus G... [more]
IPYR_THELI2.240e-1961.54Inorganic pyrophosphatase OS=Thermococcus litorali... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-102-E02-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY691939 ID=EY691939; Name=EY691939; organism=Citrus sinensis; type=EST; length=912bp
GAAAGAGAGCGGGAAGGGAACCAGCGTCAGTTCTCTTGCTTTCCTCTACT
TAAGCGCCCGTAGAGGAACTTAACTTTAATATCTTCGCCATTACGCCTCT
CTCTTTTCGAGATCGGTGCGTTTCTTTGTGGTCTTGAAGCTTCGTTCTCG
TTAGGCCCTGTTTGGCTTTCAGTACTTGAACCGTTACGGTTATGATGGCT
CCACCAATTGAGACTTCAAGCAAAACTCCTGTCTCCCAACCTCCAGCACA
TCCTCCGCTTAATGAAAGGATACTTTCTTCAATGAGCAGGAGGTCTGTTG
CTGCACATCCTTGGCATGATCTTGAGATTGGACCTGGAGCTCCAAAGATT
TTCAATTGCGTGATTGAAATTGGGAAAGGGAGCAAGGTGAAATATGAACT
TGACAAAAAATCTGGACTGATCATGGTTGATCGTGTTCTTTACTCTTCAG
TTGTGTACCCCCATAAACTATGGTTTCATACCTCGTACTCTTTGCGAAGA
CAATGATCCCCTTGATGTCCTGATCATCATGCAGGAGCCAGTTCTTCCAG
GCTGCTTTCTTTCGGGCTAAAGGCCATCGGAATTCTGCCTATGAATTGAT
CAGGGGGAAGAAAAGAATGGATAAGATCATTTGGTGGCCGGGGCTGAAGA
ATCCCCAAGTACCGAACACTACCATGGACCTTAAGGGGCTTTCCCCCATT
TGTTTGGGGTGAAAATCCCCCGCTTTTTTGAAAAAAAACAAAAAAAATGG
AAAAAAAGGAAAGTTGCGGTTAATGGATTTTTTGGCCAACCCCTGGAGGC
GTAAAAATTTTATTTCCAGGAGTTTTATGAAACCCCTTAGGGTGGAATAT
ATTTGGGGGGAAGAGTTTGGGGGGGGGGGAACTCGTTTTGGTTCCCCCCT
GGGGGTGAAAAA
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