EY691809

Overview
NameEY691809
Unique NameEY691809
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length944
Libraries
Library NameType
Sweet orange bark, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT_POPTM (Caffeoyl-CoA O-methyltransferase OS=Populus tremuloides PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 6.863e-54
Identity = 79/97 (81.44%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKL+NAKNTMEIGVYTGYSLL TALAIP+DGKILAMDINRE YELGLP+I+KAGVAHKIDF+EGPALP+LDQMI+D +YHG
Sbjct:   59 NIMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKYHG 155

HSP 2 Score: 56.9954 bits (136), Expect = 6.863e-54
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE EC+KELRE+T KHP
Sbjct:   29 LYQYILETSVYPREPECMKELREVTAKHP 57

HSP 3 Score: 32.3426 bits (72), Expect = 6.863e-54
Identity = 22/49 (44.90%), Postives = 30/49 (61.22%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVS 567
            G+FDFIFVDADK+  +   HKR+ + LV+   G  +GY N    G  V+
Sbjct:  155 GSFDFIFVDADKD-NYINYHKRL-IELVK--VGGLIGYDNTLWNGSVVA 199
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT2_EUCGL (Caffeoyl-CoA O-methyltransferase 2 OS=Eucalyptus globulus GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 2.562e-53
Identity = 79/97 (81.44%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKLINAKNTMEIGVYTGYSLL TALA+PDDGKILAMDINRE +E+GLP+IEKAG+AHKIDFREGPALPLLDQ++QDE+ HG
Sbjct:   59 NLMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENFEIGLPVIEKAGLAHKIDFREGPALPLLDQLVQDEKNHG 155

HSP 2 Score: 53.5286 bits (127), Expect = 2.562e-53
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE E +KELRE+T KHP
Sbjct:   29 LYQYILETSVYPREPESMKELREITAKHP 57

HSP 3 Score: 33.8834 bits (76), Expect = 2.562e-53
Identity = 23/51 (45.10%), Postives = 32/51 (62.75%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVSVA 573
            GT+DFIFVDADK+  +   HKR+ ++LV+   G  +GY N    G  V+ A
Sbjct:  155 GTYDFIFVDADKD-NYINYHKRL-IDLVK--VGGLIGYDNTLWNGSVVAPA 201
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT1_POPTR (Caffeoyl-CoA O-methyltransferase 1 OS=Populus trichocarpa GN=CCOAOMT1 PE=2 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 7.346e-53
Identity = 78/97 (80.41%), Postives = 89/97 (91.75%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKL+NAKNTMEIGVYTGYSLL TALAIP+DGKILAMDINRE YELGLP+I+KAGVAHKIDF+EGPALP+LDQMI+D + HG
Sbjct:   59 NIMTTSADEGQFLNMLLKLVNAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKCHG 155

HSP 2 Score: 56.9954 bits (136), Expect = 7.346e-53
Identity = 25/29 (86.21%), Postives = 27/29 (93.10%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE EC+KELRE+T KHP
Sbjct:   29 LYQYILETSVYPREPECMKELREVTAKHP 57

HSP 3 Score: 32.3426 bits (72), Expect = 7.346e-53
Identity = 22/49 (44.90%), Postives = 30/49 (61.22%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVS 567
            G+FDFIFVDADK+  +   HKR+ + LV+   G  +GY N    G  V+
Sbjct:  155 GSFDFIFVDADKD-NYINYHKRL-IELVK--VGGLIGYDNTLWNGSVVA 199
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT2_POPTR (Caffeoyl-CoA O-methyltransferase 2 OS=Populus trichocarpa GN=CCOAOMT2 PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 7.346e-53
Identity = 76/97 (78.35%), Postives = 89/97 (91.75%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKLINAKNTMEIGV+TGYSLL TALAIP+DGKILAMDINRE YELGLP+I+KAG+ HKI+F+EGPALP+LDQMI+D +YHG
Sbjct:   59 NIMTTSADEGQFLNMLLKLINAKNTMEIGVFTGYSLLATALAIPEDGKILAMDINRENYELGLPVIQKAGLEHKIEFKEGPALPVLDQMIEDGKYHG 155

HSP 2 Score: 58.151 bits (139), Expect = 7.346e-53
Identity = 26/29 (89.66%), Postives = 27/29 (93.10%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE EC+KELRELT KHP
Sbjct:   29 LYQYILETSVYPREPECMKELRELTAKHP 57

HSP 3 Score: 33.4982 bits (75), Expect = 7.346e-53
Identity = 23/51 (45.10%), Postives = 31/51 (60.78%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVSVA 573
            GT+DFIFVDADK+  +   HKR+ + LV+   G  +GY N    G  V+ A
Sbjct:  155 GTYDFIFVDADKD-NYINYHKRL-IELVK--VGGLIGYDNTLWNGSVVAPA 201
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT_VITVI (Caffeoyl-CoA O-methyltransferase OS=Vitis vinifera PE=2 SV=1)

HSP 1 Score: 164.466 bits (415), Expect = 9.562e-53
Identity = 79/97 (81.44%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKLINAKNTMEIGVYTGYSLL TALA+PDDGKILAMDIN+E YELGLP+I+KAGVAHKIDF+EGPALP+LDQMI+D +YHG
Sbjct:   54 NIMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINKENYELGLPVIQKAGVAHKIDFKEGPALPVLDQMIEDGKYHG 150

HSP 2 Score: 52.7582 bits (125), Expect = 9.562e-53
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE E +KELRELT +HP
Sbjct:   24 LYQYILETSVYPREPESMKELRELTAQHP 52

HSP 3 Score: 32.7278 bits (73), Expect = 9.562e-53
Identity = 22/49 (44.90%), Postives = 31/49 (63.27%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVS 567
            G+FDFIFVDADK+  +   HKR+ ++LV+   G  +GY N    G  V+
Sbjct:  150 GSFDFIFVDADKD-NYLNYHKRL-IDLVK--VGGIIGYDNTLWNGSVVA 194
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT5_TOBAC (Caffeoyl-CoA O-methyltransferase 5 OS=Nicotiana tabacum GN=CCOAOMT5 PE=2 SV=1)

HSP 1 Score: 162.925 bits (411), Expect = 9.566e-53
Identity = 79/97 (81.44%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKLINAKNTMEIGVYTGYSLL TALAIPDDGKILAMDINRE YE+GLPIIEKAGVAHKI+FREGPALP+LDQ+++D++ HG
Sbjct:   52 NLMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALAIPDDGKILAMDINRENYEIGLPIIEKAGVAHKIEFREGPALPVLDQLVEDKKNHG 148

HSP 2 Score: 53.1434 bits (126), Expect = 9.566e-53
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE E +KELRE+T KHP
Sbjct:   22 LYQYILETSVYPREPESMKELREVTAKHP 50

HSP 3 Score: 33.8834 bits (76), Expect = 9.566e-53
Identity = 23/49 (46.94%), Postives = 31/49 (63.27%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVS 567
            GT+DFIFVDADK+  +   HKRI ++LV+   G  +GY N    G  V+
Sbjct:  148 GTYDFIFVDADKD-NYINYHKRI-IDLVK--VGGLIGYDNTLWNGSVVA 192
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT_PETCR (Caffeoyl-CoA O-methyltransferase OS=Petroselinum crispum PE=1 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 1.245e-52
Identity = 79/97 (81.44%), Postives = 88/97 (90.72%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKLINAKNTMEIGVYTGYSLL TALA+PDDGKILAMDINRE YE+GLPIIEKAGV HKIDFREGPALP+LD M++D +YHG
Sbjct:   53 NLMTTSADEGQFLNMLLKLINAKNTMEIGVYTGYSLLATALALPDDGKILAMDINRENYEIGLPIIEKAGVGHKIDFREGPALPVLDHMLEDGKYHG 149

HSP 2 Score: 53.5286 bits (127), Expect = 1.245e-52
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE E +KELRE+T KHP
Sbjct:   23 LYQYILETSVYPREPEAMKELREVTAKHP 51

HSP 3 Score: 32.7278 bits (73), Expect = 1.245e-52
Identity = 20/40 (50.00%), Postives = 28/40 (70.00%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKN 540
            GTFDF+FVDADK+  +   HKR+ ++LV+   G  +GY N
Sbjct:  149 GTFDFVFVDADKD-NYINYHKRL-IDLVK--IGGLIGYDN 184
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT_SOLTU (Caffeoyl-CoA O-methyltransferase OS=Solanum tuberosum GN=CCOAOMT PE=2 SV=1)

HSP 1 Score: 163.696 bits (413), Expect = 1.619e-52
Identity = 78/97 (80.41%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL+MLLKLINAKNTMEIGV+TGYSLL TA+A+PDDGKILAMDINRE YE+GLP+IEKAG+AHKIDFREGPALP+LDQMI+D +YHG
Sbjct:   54 NLMTTSADEGQFLNMLLKLINAKNTMEIGVFTGYSLLATAMALPDDGKILAMDINRENYEIGLPVIEKAGLAHKIDFREGPALPVLDQMIEDGKYHG 150

HSP 2 Score: 53.9138 bits (128), Expect = 1.619e-52
Identity = 24/29 (82.76%), Postives = 26/29 (89.66%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE E +KELRE+T KHP
Sbjct:   24 LYQYILETSVYPREPEAMKELREITAKHP 52

HSP 3 Score: 31.5722 bits (70), Expect = 1.619e-52
Identity = 21/49 (42.86%), Postives = 31/49 (63.27%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVS 567
            G++DFIFVDADK+  +   HKR+ ++LV+   G  +GY N    G  V+
Sbjct:  150 GSYDFIFVDADKD-NYLNYHKRL-IDLVK--VGGLIGYDNTLWNGSVVA 194
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT_MEDSA (Caffeoyl-CoA O-methyltransferase OS=Medicago sativa GN=CCOMT PE=1 SV=1)

HSP 1 Score: 160.999 bits (406), Expect = 2.741e-52
Identity = 79/97 (81.44%), Postives = 89/97 (91.75%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFLSMLLKLINAKNTMEIGVYTGYSLL TALAIP+DGKILAMDIN+E YELGLP+I+KAGV HKIDFREGPALP+LD+MI+DE+ HG
Sbjct:   59 NIMTTSADEGQFLSMLLKLINAKNTMEIGVYTGYSLLATALAIPEDGKILAMDINKENYELGLPVIKKAGVDHKIDFREGPALPVLDEMIKDEKNHG 155

HSP 2 Score: 55.8398 bits (133), Expect = 2.741e-52
Identity = 24/29 (82.76%), Postives = 27/29 (93.10%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSV+PREHE +KELRE+T KHP
Sbjct:   29 LYQYILETSVFPREHEAMKELREVTAKHP 57

HSP 3 Score: 31.5722 bits (70), Expect = 2.741e-52
Identity = 21/49 (42.86%), Postives = 31/49 (63.27%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVS 567
            G++DFIFVDADK+  +   HKR+ ++LV+   G  +GY N    G  V+
Sbjct:  155 GSYDFIFVDADKD-NYLNYHKRL-IDLVK--VGGVIGYDNTLWNGSVVA 199
[Close]
BLAST of EY691809 vs. ExPASy Swiss-Prot
Match: CAMT_ZINEL (Caffeoyl-CoA O-methyltransferase OS=Zinnia elegans GN=CCOAOMT PE=2 SV=1)

HSP 1 Score: 163.31 bits (412), Expect = 3.567e-52
Identity = 79/97 (81.44%), Postives = 90/97 (92.78%), Query Frame = 3 
Query:  132 NHMLSTPDEAQFLSMLLKLINAKNTMEIGVYTGYSLLYTALAIPDDGKILAMDINREYYELGLPIIEKAGVAHKIDFREGPALPLLDQMIQDEEYHG 422
            N M ++ DE QFL++LLKLINAKNTMEIGVYTGYSLL TALA+P+DGKILA+DINRE YE+GLPII+KAGVAHKIDFREGPALPLLDQM+QDE+ HG
Sbjct:   57 NLMTTSADEGQFLNLLLKLINAKNTMEIGVYTGYSLLSTALALPEDGKILALDINRENYEIGLPIIQKAGVAHKIDFREGPALPLLDQMLQDEKCHG 153

HSP 2 Score: 49.6766 bits (117), Expect = 3.567e-52
Identity = 22/29 (75.86%), Postives = 25/29 (86.21%), Query Frame = 2 
Query:    2 LYQYILETSVYPREHECLKELRELTKKHP 88
            LYQYILETSVYPRE + +KELR +T KHP
Sbjct:   27 LYQYILETSVYPREPQPMKELRRITAKHP 55

HSP 3 Score: 35.039 bits (79), Expect = 3.567e-52
Identity = 23/51 (45.10%), Postives = 33/51 (64.71%), Query Frame = 1 
Query:  421 GTFDFIFVDADKNKQHSTNHKRIYLNLVERVWGE*MGYKNHAVGGESVSVA 573
            G+FDFIFVDADK+  +   HKR+ ++LV+  +G  +GY N    G  V+ A
Sbjct:  153 GSFDFIFVDADKD-NYLNYHKRL-IDLVK--FGGVIGYDNTLWNGSLVAPA 199
The following BLAST results are available for this feature:
BLAST of EY691809 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 33
Match NameE-valueIdentityDescription
CAMT_POPTM6.863e-5481.44Caffeoyl-CoA O-methyltransferase OS=Populus tremul... [more]
CAMT2_EUCGL2.562e-5381.44Caffeoyl-CoA O-methyltransferase 2 OS=Eucalyptus g... [more]
CAMT1_POPTR7.346e-5380.41Caffeoyl-CoA O-methyltransferase 1 OS=Populus tric... [more]
CAMT2_POPTR7.346e-5378.35Caffeoyl-CoA O-methyltransferase 2 OS=Populus tric... [more]
CAMT_VITVI9.562e-5381.44Caffeoyl-CoA O-methyltransferase OS=Vitis vinifera... [more]
CAMT5_TOBAC9.566e-5381.44Caffeoyl-CoA O-methyltransferase 5 OS=Nicotiana ta... [more]
CAMT_PETCR1.245e-5281.44Caffeoyl-CoA O-methyltransferase OS=Petroselinum c... [more]
CAMT_SOLTU1.619e-5280.41Caffeoyl-CoA O-methyltransferase OS=Solanum tubero... [more]
CAMT_MEDSA2.741e-5281.44Caffeoyl-CoA O-methyltransferase OS=Medicago sativ... [more]
CAMT_ZINEL3.567e-5281.44Caffeoyl-CoA O-methyltransferase OS=Zinnia elegans... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C2-003-032-F12-CT.F Sweet orange bark, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY691809 ID=EY691809; Name=EY691809; organism=Citrus sinensis; type=EST; length=944bp
GCTGTACCAGTATATACTCGAAACTAGTGTATATCCAAGAGAGCATGAAT
GTTTGAAGGAGCTCCGCGAGTTGACAAAAAAGCATCCACGGTGAGCTTCT
CGTTAATTACGTAATTAATATATAGGACAGGAACCACATGTTGAGCACAC
CTGATGAAGCACAATTCCTGAGCATGCTACTCAAGCTTATCAACGCCAAG
AATACCATGGAAATTGGTGTTTACACTGGTTACTCTCTCCTATACACTGC
CCTTGCTATTCCCGACGACGGCAAGATATTGGCCATGGACATCAATAGAG
AATACTACGAACTCGGTCTCCCCATAATTGAAAAGGCTGGTGTTGCTCAT
AAGATTGACTTCAGAGAGGGCCCTGCTCTTCCTCTTCTTGATCAAATGAT
CCAGGACGAAGAATATCATGGGGACATTTGACTTTATCTTTGTTGATGCT
GACAAGAATAAACAACATTCAACTAACCACAAGAGGATTTATTTGAACTT
GGTTGAAAGGGTTTGGGGGGAGTGAATGGGCTACAAAAACCACGCTGTTG
GGGGGGAATCTGTATCTGTGGCACCGGGATTCTATAAGGGAATGAAAGAC
TCCAGAAAATTTCCGGGAATTTACGAATGGAATTCAACAAAAGGTTGGGT
TCTTGGATCACAGAGATCTAAATTTTCTCAATGTCTCTTATTTGCGAAAG
GGGGGTTTTTTTAAGCCTGTCCGAATTGAGTAAATCCCATTATTGATCCC
CTGTGATTATTAGCAAAAAAACATTTAGTTCTGCACACACCATAGGACAA
AGATGGGGATTAAAGAAACGGGCCTAGATCGCAGTTCAAAAAGAACAGAA
AATAAAACTATTTATAGGATAAAATAGAAGAGAAAAGGAAGGGTGGGGGG
CGGTTAGGAGGATTGAAGAAGGAGAGAGATCAGACGATATAATG
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