EY710335 | Citrus Genome Database

Overview


Name	EY710335
Unique Name	EY710335
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	1048

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

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BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKH_TOBAC (Phosphoglycerate kinase, chloroplastic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 8.416e-52
Identity = 81/103 (78.64%), Postives = 88/103 (85.44%), Query Frame = 3

Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            AKKSV  L  A+LKGK+VFVR DLNVPLDDN NITDD R+RAAVPTIK+LM +GAKVILSSHLGRPKGVTPKYSL PLVPRLSELLG++V    DC G EVEK
Sbjct:   77 AKKSVGDLTAAELKGKKVFVRADLNVPLDDNQNITDDTRIRAAVPTIKHLMANGAKVILSSHLGRPKGVTPKYSLAPLVPRLSELLGIQVVKVEDCIGPEVEK 179

HSP 2 Score: 53.1434 bits (126), Expect = 8.416e-52
Identity = 22/32 (68.75%), Postives = 31/32 (96.88%), Query Frame = 1

Query:  388 KLKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            +++K+VA +PEGGVLLLENVRF++EEEKN+P+
Sbjct:  176 EVEKLVASLPEGGVLLLENVRFYKEEEKNEPE 207

HSP 3 Score: 31.187 bits (69), Expect = 8.416e-52
Identity = 17/33 (51.52%), Postives = 22/33 (66.67%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P+FAKKLASLADLY        AH +HA
Sbjct:  201 EEKNEPEFAKKLASLADLYVNDAFG-TAHRAHA 232

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKH_SPIOL (Phosphoglycerate kinase, chloroplastic (Fragment) OS=Spinacia oleracea PE=2 SV=2)

HSP 1 Score: 159.073 bits (401), Expect = 1.097e-51
Identity = 78/103 (75.73%), Postives = 90/103 (87.38%), Query Frame = 3

Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            AKKSV  L  ADLKGK+VFVR DLNVPLDD+ NITDD R+RAA+PTIK+L+ +GAKVILSSHLGRPKGVTPK+SL PLVPRLSELLG++V  A+DC G +VEK
Sbjct:   30 AKKSVGDLTSADLKGKKVFVRADLNVPLDDSQNITDDTRIRAAIPTIKHLINNGAKVILSSHLGRPKGVTPKFSLAPLVPRLSELLGLQVVKADDCIGPDVEK 132

HSP 2 Score: 56.225 bits (134), Expect = 1.097e-51
Identity = 24/31 (77.42%), Postives = 31/31 (100.00%), Query Frame = 1

Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            ++K+VAE+PEGGVLLLENVRF++EEEKNDP+
Sbjct:  130 VEKLVAELPEGGVLLLENVRFYKEEEKNDPE 160

HSP 3 Score: 31.187 bits (69), Expect = 1.097e-51
Identity = 17/33 (51.52%), Postives = 22/33 (66.67%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P+FAKKLASLADLY        AH +HA
Sbjct:  154 EEKNDPEFAKKLASLADLYVNDAFG-TAHRAHA 185

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKY_WHEAT (Phosphoglycerate kinase, cytosolic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 4.099e-51
Identity = 81/105 (77.14%), Postives = 90/105 (85.71%), Query Frame = 3

Query:   84 MAAKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            MA K+SV  L EADLKGK+VFVR DLNVPLDD   ITDD R+RA++PTIKYL+  GAKVIL+SHLGRPKGVTPK+SLKPLV RLSELLG+EV MA DC GEEVEK
Sbjct:    1 MATKRSVGTLGEADLKGKKVFVRADLNVPLDDAQKITDDTRIRASIPTIKYLLEKGAKVILASHLGRPKGVTPKFSLKPLVARLSELLGLEVVMAPDCIGEEVEK 105

HSP 2 Score: 51.9878 bits (123), Expect = 4.099e-51
Identity = 21/33 (63.64%), Postives = 31/33 (93.94%), Query Frame = 1

Query:  385 KKLKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            ++++K+ A +P+GGVLLLENVRF++EEEKNDP+
Sbjct:  101 EEVEKLAAALPDGGVLLLENVRFYKEEEKNDPE 133

HSP 3 Score: 30.0314 bits (66), Expect = 4.099e-51
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P+FAKKLAS+ADLY        AH +HA
Sbjct:  127 EEKNDPEFAKKLASVADLYVNDAFG-TAHRAHA 158

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKH_ARATH (Phosphoglycerate kinase, chloroplastic OS=Arabidopsis thaliana GN=At1g56190 PE=1 SV=2)

HSP 1 Score: 159.458 bits (402), Expect = 7.388e-50
Identity = 78/102 (76.47%), Postives = 88/102 (86.27%), Query Frame = 3

Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVE 395
            AKKSV  L   DLKGK+VFVR DLNVPLDDN NITDD R+RAA+PTIK+L+ +GAKVILS+HLGRPKGVTPK+SL PLVPRLSELLG+EV  A+DC G EVE
Sbjct:   75 AKKSVGDLNSVDLKGKKVFVRADLNVPLDDNQNITDDTRIRAAIPTIKFLIENGAKVILSTHLGRPKGVTPKFSLAPLVPRLSELLGIEVVKADDCIGPEVE 176

HSP 2 Score: 50.447 bits (119), Expect = 7.388e-50
Identity = 21/31 (67.74%), Postives = 29/31 (93.55%), Query Frame = 1

Query:  388 KLKKMVAEIPEGGVLLLENVRFHREEEKNDP 480
            +++ +VA +PEGGVLLLENVRF++EEEKN+P
Sbjct:  174 EVETLVASLPEGGVLLLENVRFYKEEEKNEP 204

HSP 3 Score: 30.4166 bits (67), Expect = 7.388e-50
Identity = 17/33 (51.52%), Postives = 21/33 (63.64%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P FAKKLASLADLY        AH +HA
Sbjct:  199 EEKNEPDFAKKLASLADLYVNDAFG-TAHRAHA 230

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKY_TOBAC (Phosphoglycerate kinase, cytosolic OS=Nicotiana tabacum PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 2.862e-44
Identity = 90/105 (85.71%), Postives = 95/105 (90.48%), Query Frame = 3

Query:   84 MAAKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            MA KKSV  LKEADLKGKRVFVRVDLNVPLD+N NITDD R+RAAVPTIKYLM HG+ VIL+SHLGRPKGVTPKYSLKPLVPRLSELLGVEVK+AND  G EVEK
Sbjct:    1 MAVKKSVGSLKEADLKGKRVFVRVDLNVPLDENFNITDDTRIRAAVPTIKYLMQHGSHVILASHLGRPKGVTPKYSLKPLVPRLSELLGVEVKIANDSIGPEVEK 105

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKH_WHEAT (Phosphoglycerate kinase, chloroplastic OS=Triticum aestivum PE=2 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 4.069e-41
Identity = 80/103 (77.67%), Postives = 88/103 (85.44%), Query Frame = 3

Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            AKKSV  L  ADL+GKRV VR DLNVPLDDN NITDD R+RAA+PTIKYL+ +GAKVIL+SHLGRPKGVTPK+SL PLVPRLSELLG+EVK A D  G EVEK
Sbjct:   74 AKKSVGDLTAADLEGKRVLVRADLNVPLDDNQNITDDTRIRAAIPTIKYLLSNGAKVILTSHLGRPKGVTPKFSLAPLVPRLSELLGIEVKKAEDVIGPEVEK 176

HSP 2 Score: 29.6462 bits (65), Expect = 4.069e-41
Identity = 16/33 (48.48%), Postives = 22/33 (66.67%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P+FAKKLASLADL+        AH +HA
Sbjct:  198 EEKNDPEFAKKLASLADLFVNDAFG-TAHRAHA 229

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKH_VOLCA (Phosphoglycerate kinase, chloroplastic OS=Volvox carteri GN=PGK PE=2 SV=1)

HSP 1 Score: 131.724 bits (330), Expect = 6.664e-39
Identity = 71/106 (66.98%), Postives = 82/106 (77.36%), Query Frame = 3

Query:   87 AAKKSVSVLKEADLKGKRVFVRVDLNVPLDDN-LNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPK-GVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            A KKSV  L +ADL+GKRVFVR DLNVPLD   L ITDD R+RAAVPT+KYL+ +GAKV+L+SHLGRPK G   KY L P+V RLSELLG EVK  +DC G  VE+
Sbjct:   61 AVKKSVGDLGKADLEGKRVFVRADLNVPLDKKTLAITDDTRIRAAVPTLKYLLDNGAKVLLTSHLGRPKGGPEDKYRLTPVVARLSELLGKEVKKVDDCIGPSVEQ 166

HSP 2 Score: 42.3578 bits (98), Expect = 6.664e-39
Identity = 18/31 (58.06%), Postives = 26/31 (83.87%), Query Frame = 1

Query:  391 LKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            +++ VA +  G +LLLENVRF++EEEKNDP+
Sbjct:  164 VEQAVASLKSGELLLLENVRFYKEEEKNDPE 194

HSP 3 Score: 29.261 bits (64), Expect = 6.664e-39
Identity = 32/77 (41.56%), Postives = 40/77 (51.95%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHAFP*RVVG*FL*NPFCGAGFPLWQKGLNLSWLGGWFQIPKKTILA 694
            +++  P+FAKKLAS ADLY        AH +HA    V   FL  P   AGF L QK   L +L G    PK+  +A
Sbjct:  188 EEKNDPEFAKKLASNADLYVNDAFG-TAHRAHASTEGVTK-FL-KPSV-AGF-LLQK--ELDYLDGAVSAPKRPFVA 257

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_DICTD (Phosphoglycerate kinase OS=Dictyoglomus turgidum (strain Z-1310 / DSM 6724) GN=pgk PE=3 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 2.628e-37
Identity = 66/103 (64.08%), Postives = 76/103 (73.79%), Query Frame = 3

Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            AKK++  L++ DLKGKRV VRVD NVPL + + ITDD R+R A+PTIKYLM  GAKVIL SHLGRPKG      L P+  RLSELLG  VK  NDC GEEVE+
Sbjct:    2 AKKTILDLRDEDLKGKRVLVRVDFNVPLKNGV-ITDDRRIREALPTIKYLMDKGAKVILVSHLGRPKGFQDDLRLDPVAKRLSELLGKPVKKLNDCIGEEVER 103

HSP 2 Score: 41.2022 bits (95), Expect = 2.628e-37
Identity = 15/33 (45.45%), Postives = 28/33 (84.85%), Query Frame = 1

Query:  385 KKLKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            +++++ ++ + EG V+LLEN+RF++EEE NDP+
Sbjct:   99 EEVEREISNMKEGDVILLENIRFYKEEEANDPE 131

HSP 3 Score: 30.8018 bits (68), Expect = 2.628e-37
Identity = 17/28 (60.71%), Postives = 19/28 (67.86%), Query Frame = 2

Query:  479 PKFAKKLASLADLYGKGRLSDLAH*SHA 562
            P+FAKKLASLADLY        AH +HA
Sbjct:  130 PEFAKKLASLADLYVNDAFG-TAHRAHA 156

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGKH_CHLRE (Phosphoglycerate kinase, chloroplastic OS=Chlamydomonas reinhardtii PE=1 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 4.423e-37
Identity = 72/109 (66.06%), Postives = 82/109 (75.23%), Query Frame = 3

Query:   87 AAKKSVSVLKEADLKGKRVFVRVDLNVPLDD-NLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPK-GVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEKNGG 407
            A KKSV  L +ADL+GKRVFVR DLNVPLD   L ITDD R+RAAVPT+KYL+ +GAKV+L+SHLGRPK G   KY L P+V RLSELLG  V   +DC G EVEK  G
Sbjct:   60 AVKKSVGDLHKADLEGKRVFVRADLNVPLDKATLAITDDTRIRAAVPTLKYLLDNGAKVLLTSHLGRPKGGPEDKYRLTPVVARLSELLGKPVTKVDDCIGPEVEKAVG 168

HSP 2 Score: 38.5058 bits (88), Expect = 4.423e-37
Identity = 16/32 (50.00%), Postives = 25/32 (78.12%), Query Frame = 1

Query:  388 KLKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            +++K V  +  G +LLLEN RF++EEEKN+P+
Sbjct:  162 EVEKAVGAMKNGELLLLENCRFYKEEEKNEPE 193

HSP 3 Score: 27.335 bits (59), Expect = 4.423e-37
Identity = 15/33 (45.45%), Postives = 21/33 (63.64%), Query Frame = 2

Query:  464 KKRMTPKFAKKLASLADLYGKGRLSDLAH*SHA 562
            +++  P+FAKKLA+ ADLY        AH +HA
Sbjct:  187 EEKNEPEFAKKLAANADLYVNDAFG-TAHRAHA 218

[Close]

BLAST of EY710335 vs. ExPASy Swiss-Prot
Match: PGK_DICT6 (Phosphoglycerate kinase OS=Dictyoglomus thermophilum (strain ATCC 35947 / DSM 3960 / H-6-12) GN=pgk PE=3 SV=1)

HSP 1 Score: 123.25 bits (308), Expect = 2.780e-36
Identity = 64/103 (62.14%), Postives = 76/103 (73.79%), Query Frame = 3

Query:   90 AKKSVSVLKEADLKGKRVFVRVDLNVPLDDNLNITDDNRVRAAVPTIKYLMGHGAKVILSSHLGRPKGVTPKYSLKPLVPRLSELLGVEVKMANDCFGEEVEK 398
            AKK++  L++ +LKGKRV VRVD NVP+ + + ITDD R+R A+PTIKYL+  GAKVIL SHLGRPKG      L P+  RLSELLG  VK  NDC GEEVEK
Sbjct:    2 AKKTILDLQDEELKGKRVLVRVDFNVPIKNGV-ITDDRRIREALPTIKYLIDRGAKVILVSHLGRPKGFQDDLRLDPVAKRLSELLGKPVKKLNDCIGEEVEK 103

HSP 2 Score: 41.9726 bits (97), Expect = 2.780e-36
Identity = 16/33 (48.48%), Postives = 28/33 (84.85%), Query Frame = 1

Query:  385 KKLKKMVAEIPEGGVLLLENVRFHREEEKNDPK 483
            ++++K ++ + EG V+LLEN+RF++EEE NDP+
Sbjct:   99 EEVEKEISNMKEGDVVLLENIRFYKEEEANDPE 131

HSP 3 Score: 29.261 bits (64), Expect = 2.780e-36
Identity = 16/28 (57.14%), Postives = 18/28 (64.29%), Query Frame = 2

Query:  479 PKFAKKLASLADLYGKGRLSDLAH*SHA 562
            P+FAKKLA LADLY        AH +HA
Sbjct:  130 PEFAKKLAKLADLYVNDAFG-TAHRAHA 156

The following BLAST results are available for this feature:

BLAST of EY710335 vs. ExPASy Swiss-Prot

BLAST of EY710335 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
PGKH_TOBAC	8.416e-52	78.64	Phosphoglycerate kinase, chloroplastic OS=Nicotian...	[more]
PGKH_SPIOL	1.097e-51	75.73	Phosphoglycerate kinase, chloroplastic (Fragment) ...	[more]
PGKY_WHEAT	4.099e-51	77.14	Phosphoglycerate kinase, cytosolic OS=Triticum aes...	[more]
PGKH_ARATH	7.388e-50	76.47	Phosphoglycerate kinase, chloroplastic OS=Arabidop...	[more]
PGKY_TOBAC	2.862e-44	85.71	Phosphoglycerate kinase, cytosolic OS=Nicotiana ta...	[more]
PGKH_WHEAT	4.069e-41	77.67	Phosphoglycerate kinase, chloroplastic OS=Triticum...	[more]
PGKH_VOLCA	6.664e-39	66.98	Phosphoglycerate kinase, chloroplastic OS=Volvox c...	[more]
PGK_DICTD	2.628e-37	64.08	Phosphoglycerate kinase OS=Dictyoglomus turgidum (...	[more]
PGKH_CHLRE	4.423e-37	66.06	Phosphoglycerate kinase, chloroplastic OS=Chlamydo...	[more]
PGK_DICT6	2.780e-36	62.14	Phosphoglycerate kinase OS=Dictyoglomus thermophil...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-701-097-F04-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY710335 ID=EY710335; Name=EY710335; organism=Citrus sinensis; type=EST; length=1048bp
CGTTCACCAATCTTCGATCTCATTCCAAAATCTAACCAAAACCCTTTCTC
GATTTCTCTGATCTCGTGTTTGATCGAATCAAAATGGCCGCCAAGAAGAG
TGTAAGCGTTCTAAAAGAAGCTGATTTGAAGGGAAAGAGAGTGTTCGTAC
GAGTGGATCTGAACGTGCCGTTGGATGACAACTTGAATATCACCGATGAC
AACAGAGTCCGTGCTGCTGTTCCCACTATCAAATACCTGATGGGTCATGG
CGCCAAAGTCATTCTCTCCAGTCACTTGGGGCGTCCAAAGGGTGTTACAC
CCAAGTACAGCTTGAAACCTCTTGTGCCAAGGCTTTCTGAACTTCTTGGA
GTTGAGGTTAAAATGGCAAACGACTGCTTTGGTGAAGAAGTTGAAAAAAA
TGGTGGCTGAAATTCCCGAAGGGGGCGTCTTGCTCCTCGAAAATGTGAGG
TTCCATAGGGAGGAAGAAAAGAATGACCCCAAAGTTTGCTAAGAAGCTAG
CTTCCCTGGCAGATCTCTATGGTAAAGGAAGGCTTTCGGATCTGGCCCAT
TAAAGCCATGCTTTCCCCTGAAGGGTGGTGGGCTAATTTCTTTGAAACCC
TTTCTGTGGGGCTGGGTTTCCTTTATGGCAAAAGGGACTAAATTTATCTT
GGTTGGGAGGCTGGTTTCAAATCCCCAAAAAAACCATTTTGGCGGCCCTT
TGTTGGGGGGGTTTAAAAAAGGGGGAAAACTAAAAAAATGGGGGTTTATT
GGATTCCCTTTTTGGGGAAAAAGGGTGAACATTTTTATTTTGCCCGGGGG
GAAAGAATATAATTTTTATCACCTTTATCACAGGGCCCAAAGGGAACAAC
CTTTGGTTGGGTATCATCCCCTTGTGTGAAGGGAAAAAAAAAAAGTTTTT
GTTTACTCCCCCACATTTGGCCCTTTTATGGGAAAAAAGAGGGGACAGAT
TCTTAAAAAGAGGGGGGTGTGGTCTCTATCTATGTATACTCCCCTCTCTA
AGTATGTGGTGTGTTGTTTTTTTTTGTTTTTTGTACGATCAAAAAAAT