EY709989

Overview
NameEY709989
Unique NameEY709989
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1132
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP27_ARATH (Aquaporin PIP2-7 OS=Arabidopsis thaliana GN=PIP2-7 PE=1 SV=2)

HSP 1 Score: 134.035 bits (336), Expect = 5.506e-42
Identity = 62/80 (77.50%), Postives = 70/80 (87.50%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDANT 536
            LGIAWAFGGMIFVLVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y++AQCLGA+CGVG  KAF+K  Y  +GG ANT
Sbjct:   74 LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALGYMIAQCLGAICGVGFVKAFMKTPYNTLGGGANT 153

HSP 2 Score: 42.743 bits (99), Expect = 5.506e-42
Identity = 19/28 (67.86%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQIDACGGVGLFG 301
            +LFLYV+VA+VIGH KQ   C GVGL G
Sbjct:   48 LLFLYVTVATVIGHKKQTGPCDGVGLLG 75

HSP 3 Score: 37.3502 bits (85), Expect = 5.506e-42
Identity = 21/50 (42.00%), Postives = 26/50 (52.00%), Query Frame = 1 
Query:   67 MSYEVNEQGQTHRHHHGQXXXXXXXXXXXXXXXXXXWSFY*ALIAHFVAT 216
            MS EV+E+G+TH   HG+                  WSFY ALIA F+AT
Sbjct:    1 MSKEVSEEGKTH---HGKDYVDPPPAPLLDMGELKSWSFYRALIAEFIAT 47
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP1_ATRCA (Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1)

HSP 1 Score: 131.339 bits (329), Expect = 6.974e-38
Identity = 61/90 (67.78%), Postives = 71/90 (78.89%), Query Frame = 3 
Query:  276 PVVVLACLGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDANTCGL 545
            P   +  LGIAW+FGGMIFVLVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y++AQC GA+CGVG  KAF+K  Y   GG AN+  L
Sbjct:   69 PCASVGLLGIAWSFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLLRALVYMIAQCAGAICGVGLVKAFMKGPYNQFGGGANSVAL 158

HSP 2 Score: 39.6614 bits (91), Expect = 6.974e-38
Identity = 16/28 (57.14%), Postives = 22/28 (78.57%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQIDACGGVGLFG 301
            +LFLY++VA+VIG+ K+ D C  VGL G
Sbjct:   50 LLFLYITVATVIGYKKETDPCASVGLLG 77

HSP 3 Score: 29.261 bits (64), Expect = 6.974e-38
Identity = 19/51 (37.25%), Postives = 25/51 (49.02%), Query Frame = 1 
Query:   67 MSYEV-NEQGQTHRHHHGQXXXXXXXXXXXXXXXXXXWSFY*ALIAHFVAT 216
            MS EV +E+GQ H+H  G+                  WSF+ A IA F+AT
Sbjct:    1 MSKEVISEEGQVHQH--GKDYVDPPPAPFFDMGELKLWSFWRAAIAEFIAT 49
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP21_ORYSJ (Probable aquaporin PIP2-1 OS=Oryza sativa subsp. japonica GN=PIP2-1 PE=2 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 8.242e-35
Identity = 60/80 (75.00%), Postives = 67/80 (83.75%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDANT 536
            LGIAWAFGGMIF+LVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y+VAQCLGA+CGVG  KAF    +   GG ANT
Sbjct:   86 LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILYIVAQCLGAICGVGLVKAFQSAYFNRYGGGANT 165

HSP 2 Score: 36.5798 bits (83), Expect = 8.242e-35
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID--------ACGGVGLFG 301
            +LFLY++VA+VIG+  Q D        ACGGVG+ G
Sbjct:   52 LLFLYITVATVIGYKHQTDASASGADAACGGVGVLG 87

HSP 3 Score: 23.483 bits (49), Expect = 8.242e-35
Identity = 9/14 (64.29%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA F+AT
Sbjct:   38 WSLYRAVIAEFIAT 51
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP22_MAIZE (Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 2.349e-34
Identity = 68/117 (58.12%), Postives = 79/117 (67.52%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDANTCGLVATPKDMPLGGGNLLVPSLACFQRPLRQQTPKKN 647
            LGIAWAFGGMIFVLVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y+VAQCLGAVCGVG  KAF    +   GG AN+   +A+      G G  +V +            PK+N
Sbjct:   88 LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYMVAQCLGAVCGVGLVKAFQSAYFDRYGGGANS---LASGYSRGAGLGAEIVGTFVLVYTVFSATDPKRN 201

HSP 2 Score: 36.1946 bits (82), Expect = 2.349e-34
Identity = 19/38 (50.00%), Postives = 24/38 (63.16%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID----------ACGGVGLFG 301
            +LFLYV+VA+VIG+  Q D          ACGGVG+ G
Sbjct:   52 LLFLYVTVATVIGYKHQTDASASGAGADAACGGVGVLG 89

HSP 3 Score: 23.483 bits (49), Expect = 2.349e-34
Identity = 9/14 (64.29%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA F+AT
Sbjct:   38 WSLYRAVIAEFIAT 51
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP21_MAIZE (Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2)

HSP 1 Score: 127.872 bits (320), Expect = 3.052e-34
Identity = 66/117 (56.41%), Postives = 79/117 (67.52%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDANTCGLVATPKDMPLGGGNLLVPSLACFQRPLRQQTPKKN 647
            LGIAWAFGGMIFVLVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y+VAQCLGA+CGVG  KAF    +   GG AN+   +A+      G G  ++ +            PK+N
Sbjct:   86 LGIAWAFGGMIFVLVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGLVKAFQSAYFDRYGGGANS---LASGYSRGTGLGAEIIGTFVLVYTVFSATDPKRN 199

HSP 2 Score: 36.5798 bits (83), Expect = 3.052e-34
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID--------ACGGVGLFG 301
            +LFLY++VA+VIG+  Q D        ACGGVG+ G
Sbjct:   52 LLFLYITVATVIGYKHQTDASASGADAACGGVGVLG 87

HSP 3 Score: 23.483 bits (49), Expect = 3.052e-34
Identity = 9/14 (64.29%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA F+AT
Sbjct:   38 WSLYRAVIAEFIAT 51
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP21_ARATH (Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1 PE=1 SV=1)

HSP 1 Score: 128.642 bits (322), Expect = 3.053e-34
Identity = 60/80 (75.00%), Postives = 67/80 (83.75%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDANT 536
            LGIAWAFGGMIF+LVYC +GISGGHINPAVTFGLFLARKVSL  A+ Y++AQCLGA+CGVG  KAF    YT  GG AN+
Sbjct:   81 LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLPRALLYIIAQCLGAICGVGFVKAFQSSYYTRYGGGANS 160

HSP 2 Score: 33.113 bits (74), Expect = 3.053e-34
Identity = 16/34 (47.06%), Postives = 22/34 (64.71%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQIDA------CGGVGLFG 301
            +LFLY++V +VIG+  Q D       CGGVG+ G
Sbjct:   49 LLFLYITVLTVIGYKIQSDTDAGGVDCGGVGILG 82

HSP 3 Score: 26.1794 bits (56), Expect = 3.053e-34
Identity = 11/14 (78.57%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WSFY A+IA FVAT
Sbjct:   35 WSFYRAVIAEFVAT 48
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP25_MAIZE (Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1)

HSP 1 Score: 127.102 bits (318), Expect = 3.969e-34
Identity = 58/79 (73.42%), Postives = 65/79 (82.28%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDAN 533
            LGIAWAFGGMIF+LVYC +G+SGGHINPAVTFGLFLARKVSL+ A+ Y+VAQCLGA+CGVG  K F    Y   GG AN
Sbjct:   81 LGIAWAFGGMIFILVYCTAGVSGGHINPAVTFGLFLARKVSLVRALLYIVAQCLGAICGVGLVKGFQSAFYVRYGGGAN 159

HSP 2 Score: 36.5798 bits (83), Expect = 3.969e-34
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID--------ACGGVGLFG 301
            +LFLY++VA+VIG+  Q D        ACGGVG+ G
Sbjct:   47 LLFLYITVATVIGYKHQTDAAASGPDAACGGVGVLG 82

HSP 3 Score: 23.8682 bits (50), Expect = 3.969e-34
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA FVAT
Sbjct:   33 WSLYRAVIAEFVAT 46
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP24_MAIZE (Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 5.155e-34
Identity = 58/79 (73.42%), Postives = 65/79 (82.28%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDAN 533
            LGIAWAFGGMIF+LVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y++AQCLGA+CGVG  K F    Y   GG AN
Sbjct:   85 LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGAN 163

HSP 2 Score: 36.965 bits (84), Expect = 5.155e-34
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID--------ACGGVGLFG 301
            +LFLY++VA+VIG+  Q D        ACGGVG+ G
Sbjct:   51 LLFLYITVATVIGYKHQTDASASGPDAACGGVGILG 86

HSP 3 Score: 23.483 bits (49), Expect = 5.155e-34
Identity = 9/14 (64.29%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA F+AT
Sbjct:   37 WSLYRAVIAEFIAT 50
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP23_MAIZE (Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 5.155e-34
Identity = 58/79 (73.42%), Postives = 65/79 (82.28%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDAN 533
            LGIAWAFGGMIF+LVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y++AQCLGA+CGVG  K F    Y   GG AN
Sbjct:   86 LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRALLYIIAQCLGAICGVGLVKGFQSAYYVRYGGGAN 164

HSP 2 Score: 36.965 bits (84), Expect = 5.155e-34
Identity = 18/36 (50.00%), Postives = 24/36 (66.67%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID--------ACGGVGLFG 301
            +LFLY++VA+VIG+  Q D        ACGGVG+ G
Sbjct:   52 LLFLYITVATVIGYKHQTDAAASGPDAACGGVGILG 87

HSP 3 Score: 23.483 bits (49), Expect = 5.155e-34
Identity = 9/14 (64.29%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA F+AT
Sbjct:   38 WSLYRAVIAEFIAT 51
[Close]
BLAST of EY709989 vs. ExPASy Swiss-Prot
Match: PIP25_ORYSJ (Aquaporin PIP2-5 OS=Oryza sativa subsp. japonica GN=PIP2-5 PE=2 SV=1)

HSP 1 Score: 126.331 bits (316), Expect = 5.158e-34
Identity = 59/79 (74.68%), Postives = 64/79 (81.01%), Query Frame = 3 
Query:  297 LGIAWAFGGMIFVLVYCASGISGGHINPAVTFGLFLARKVSLIMAVAYLVAQCLGAVCGVG*FKAFLKQLYTHIGGDAN 533
            LGIAWAFGGMIF+LVYC +GISGGHINPAVTFGLFLARKVSL+ A+ Y+VAQCLGAVCGV   K F    Y   GG AN
Sbjct:   80 LGIAWAFGGMIFILVYCTAGISGGHINPAVTFGLFLARKVSLVRAILYIVAQCLGAVCGVALVKGFQSSFYDRYGGGAN 158

HSP 2 Score: 36.965 bits (84), Expect = 5.158e-34
Identity = 19/36 (52.78%), Postives = 24/36 (66.67%), Query Frame = 2 
Query:  218 VLFLYVSVASVIGHNKQID--------ACGGVGLFG 301
            +LFLYV+VA+VIG+  Q D        ACGGVG+ G
Sbjct:   46 LLFLYVTVATVIGYKHQTDASASGDDAACGGVGVLG 81

HSP 3 Score: 23.8682 bits (50), Expect = 5.158e-34
Identity = 10/14 (71.43%), Postives = 11/14 (78.57%), Query Frame = 1 
Query:  175 WSFY*ALIAHFVAT 216
            WS Y A+IA FVAT
Sbjct:   32 WSLYRAVIAEFVAT 45
The following BLAST results are available for this feature:
BLAST of EY709989 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 40
Match NameE-valueIdentityDescription
PIP27_ARATH5.506e-4277.50Aquaporin PIP2-7 OS=Arabidopsis thaliana GN=PIP2-7... [more]
PIP1_ATRCA6.974e-3867.78Aquaporin PIP-type OS=Atriplex canescens PE=2 SV=1[more]
PIP21_ORYSJ8.242e-3575.00Probable aquaporin PIP2-1 OS=Oryza sativa subsp. j... [more]
PIP22_MAIZE2.349e-3458.12Aquaporin PIP2-2 OS=Zea mays GN=PIP2-2 PE=2 SV=1[more]
PIP21_MAIZE3.052e-3456.41Aquaporin PIP2-1 OS=Zea mays GN=PIP2-1 PE=1 SV=2[more]
PIP21_ARATH3.053e-3475.00Aquaporin PIP2-1 OS=Arabidopsis thaliana GN=PIP2-1... [more]
PIP25_MAIZE3.969e-3473.42Aquaporin PIP2-5 OS=Zea mays GN=PIP2-5 PE=1 SV=1[more]
PIP24_MAIZE5.155e-3473.42Aquaporin PIP2-4 OS=Zea mays GN=PIP2-4 PE=1 SV=1[more]
PIP23_MAIZE5.155e-3473.42Aquaporin PIP2-3 OS=Zea mays GN=PIP2-3 PE=2 SV=1[more]
PIP25_ORYSJ5.158e-3474.68Aquaporin PIP2-5 OS=Oryza sativa subsp. japonica G... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-022-F09-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY709989 ID=EY709989; Name=EY709989; organism=Citrus sinensis; type=EST; length=1132bp
TTTTTTGGGATCCGGCAGGTACGGTCCCGGATTCCCGGGGCAGCACACGA
ATACGAATAAAGAAAGATGTCATACGAAGTGAATGAACAAGGACAAACTC
ACCGCCATCACCATGGTCAGGACTACCTGGACCCACCACCACCACCACTC
ATTGACATGGCTGACCTCTAGCTCTGGTCCTTTTACTGAGCTCTCATAGC
CCACTTCGTGGCCACTCGTCCTCTTCCTCTACGTCAGTGTTGCCAGTGTC
ATTGGCCACAATAAACAAATTGATGCCTGTGGTGGTGTTGGCCTGTTTGG
GTATTGCATGGGCCTTTGGTGGCATGATCTTCGTCCTTGTTTACTGCGCC
TCGGGCATTTCTGGTGGTCACATTAACCCTGCTGTGACGTTTGGGCTGTT
CTTGGCTAGGAAGGTATCATTGATCATGGCTGTGGCTTACCTGGTGGCTC
AGTGCTTGGGTGCCGTTTGCGGCGTTGGATAGTTCAAGGCCTTCTTGAAG
CAATTGTACACTCACATTGGTGGTGATGCAAACACTTGTGGCCTTGTGGC
TACCCCCAAGGATATGCCTTTGGGTGGTGGAAATTTATTGGTACCTTCTC
TTGCTTGTTTTCAAAGACCTCTCCGGCAACAAACCCCCAAAAAAAACGGC
TCGTGACCTCTCAATTGCCCCCTTTTTGGCACATTTTCCAATTGAAATTT
CTTTGGGTTCCATGGAAAAACTTGGACCACCAAACCCAATTCCTGGAACC
GGGGGTAAAAACCGGCTTTTGGATTCTTTGGTGGTGGCTTGTCTCCTACA
AAATTTTGAGGGGGCCGGGGAATAAAACATTTGGGTCTCTCTTCGGGGTG
TTCCCTTTCCTTTGAAAGAATTTGCCAGTCCCGCCCCATAACACCCCGGA
ATACACACTTATGTGGGGGCGGTGGTGCTTTCTCAGGGGATTTTTAATAT
CAATTACACACCAATACCCCCTTTATAGTAACAGAGGAAAAATAAAAGTT
TTTTTTATAAAAAACTATGTTATCACCGACGAGAACAAATAAATACACCC
GAATTTCTACACACACCTTCTTTTTTGCGCTTAGACGCACCCACAAATAC
TGCATGGCATTTATTTATTTACGATTTCTGAA
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