EY683491

Overview
NameEY683491
Unique NameEY683491
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length722
Libraries
Library NameType
Sweet orange leaf, young greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA2_ARATH (Catalase-2 OS=Arabidopsis thaliana GN=CAT2 PE=1 SV=3)

HSP 1 Score: 397.897 bits (1021), Expect = 2.853e-112
Identity = 183/196 (93.37%), Postives = 195/196 (99.49%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK+RPASS+N+PFFTTNSGAPVWNNNSSMT+GPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+SNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFF+RDGMKFPDMVHALKPNPKSHIQENWRI+DFFSHHPESL+MF+FLF+++G P
Sbjct:    1 MDPYKYRPASSYNSPFFTTNSGAPVWNNNSSMTVGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLNMFTFLFDDIGIP 196

HSP 2 Score: 29.6462 bits (65), Expect = 2.853e-112
Identity = 11/16 (68.75%), Postives = 14/16 (87.50%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNTLL 722
            PQ+Y HM+GSGVNT +
Sbjct:  196 PQDYRHMDGSGVNTYM 211
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA3_NICPL (Catalase isozyme 3 OS=Nicotiana plumbaginifolia GN=CAT3 PE=2 SV=1)

HSP 1 Score: 388.652 bits (997), Expect = 7.613e-110
Identity = 180/196 (91.84%), Postives = 193/196 (98.47%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK+RP+S+ N+PF+TTNSGAPVWNNNSSMT+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+++LTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRI+DFFSHHPESLHMFSFLF+++G P
Sbjct:    1 MDPYKYRPSSANNSPFWTTNSGAPVWNNNSSMTVGTRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDITHLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLHMFSFLFDDLGVP 196

HSP 2 Score: 30.8018 bits (68), Expect = 7.613e-110
Identity = 12/16 (75.00%), Postives = 14/16 (87.50%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNTLL 722
            PQ+Y HMEGSGVNT +
Sbjct:  196 PQDYRHMEGSGVNTYM 211
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA_SOLAP (Catalase OS=Soldanella alpina PE=2 SV=1)

HSP 1 Score: 387.497 bits (994), Expect = 2.206e-109
Identity = 179/196 (91.33%), Postives = 192/196 (97.96%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK+R +S+FN PF+TTNSGAPVWNNNSS+T+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+S+LTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRI+DFFSHHPESLHMF+FLF+++G P
Sbjct:    1 MDPYKYRSSSAFNTPFWTTNSGAPVWNNNSSLTVGTRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISHLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLHMFTFLFDDLGVP 196

HSP 2 Score: 30.4166 bits (67), Expect = 2.206e-109
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HMEGSGVNT
Sbjct:  196 PQDYRHMEGSGVNT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA2_GOSHI (Catalase isozyme 2 OS=Gossypium hirsutum GN=CAT2 PE=2 SV=1)

HSP 1 Score: 390.193 bits (1001), Expect = 2.879e-109
Identity = 179/196 (91.33%), Postives = 193/196 (98.47%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYKFRP+SSF++PF+TTNSGAPVWNNNSS+T+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+S LTCADFLRAPGVQTP+IVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFF+RDGMKFPDMVHALKPNPKSHIQENWRI+DFFSHHPESLHMF+FLF+++G P
Sbjct:    1 MDPYKFRPSSSFDSPFWTTNSGAPVWNNNSSLTVGARGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISQLTCADFLRAPGVQTPLIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLHMFTFLFDDIGVP 196

HSP 2 Score: 27.335 bits (59), Expect = 2.879e-109
Identity = 10/14 (71.43%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HM+GSGV+T
Sbjct:  196 PQDYRHMDGSGVHT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA1_GOSHI (Catalase isozyme 1 OS=Gossypium hirsutum GN=CAT1 PE=2 SV=2)

HSP 1 Score: 387.111 bits (993), Expect = 2.879e-109
Identity = 178/196 (90.82%), Postives = 192/196 (97.96%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK RP+S+FN+PF+TTNSGAPVWNNNSS+T+GPRG  LLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+S+LTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFF+RDGMKFPDMVHALKPNPKSHIQENWRI+DFFSHHPESLHMF+FLF+++G P
Sbjct:    1 MDPYKHRPSSAFNSPFWTTNSGAPVWNNNSSLTVGPRGQYLLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISHLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLHMFTFLFDDLGVP 196

HSP 2 Score: 30.4166 bits (67), Expect = 2.879e-109
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HMEGSGVNT
Sbjct:  196 PQDYRHMEGSGVNT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA3_SOYBN (Catalase-3 OS=Glycine max GN=CAT3 PE=2 SV=1)

HSP 1 Score: 388.267 bits (996), Expect = 1.420e-108
Identity = 179/196 (91.33%), Postives = 193/196 (98.47%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK RP+S+FN+PF+TTNSGAP+WNNNSS+T+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+S+LTCADFLRAPGVQTP+IVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDG+KFPDMVHALKPNPKSHIQENWRI+DFFSHHPESLHMFSFLF++VG P
Sbjct:    1 MDPYKNRPSSAFNSPFWTTNSGAPIWNNNSSLTVGSRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISHLTCADFLRAPGVQTPLIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGLKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLHMFSFLFDDVGIP 196

HSP 2 Score: 26.9498 bits (58), Expect = 1.420e-108
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HM+G GVNT
Sbjct:  196 PQDYRHMDGFGVNT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA1_SOYBN (Catalase-1/2 OS=Glycine max GN=CAT1 PE=2 SV=1)

HSP 1 Score: 388.267 bits (996), Expect = 1.420e-108
Identity = 179/196 (91.33%), Postives = 193/196 (98.47%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK RP+S+FN+PF+TTNSGAP+WNNNSS+T+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+S+LTCADFLRAPGVQTP+IVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDG+KFPDMVHALKPNPKSHIQENWRI+DFFSHHPESLHMFSFLF++VG P
Sbjct:    1 MDPYKNRPSSAFNSPFWTTNSGAPIWNNNSSLTVGSRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISHLTCADFLRAPGVQTPLIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGLKFPDMVHALKPNPKSHIQENWRILDFFSHHPESLHMFSFLFDDVGIP 196

HSP 2 Score: 26.9498 bits (58), Expect = 1.420e-108
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HM+G GVNT
Sbjct:  196 PQDYRHMDGFGVNT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA_PHAAU (Catalase OS=Phaseolus aureus PE=2 SV=1)

HSP 1 Score: 384.415 bits (986), Expect = 2.030e-107
Identity = 178/196 (90.82%), Postives = 192/196 (97.96%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK+RP+S+FN+PF+TTNSGAPVWNNN+S+T+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVS+LTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNN PVFFVRDGMKFPDMVHALKPNPK+HIQENWRI+DFFSH PESLHMFSFLF+++G P
Sbjct:    1 MDPYKYRPSSAFNSPFWTTNSGAPVWNNNNSLTVGTRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSHLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNLPVFFVRDGMKFPDMVHALKPNPKNHIQENWRILDFFSHFPESLHMFSFLFDDLGVP 196

HSP 2 Score: 26.9498 bits (58), Expect = 2.030e-107
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HM+G GVNT
Sbjct:  196 PQDYRHMDGFGVNT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA1_CUCPE (Catalase isozyme 1 OS=Cucurbita pepo GN=CAT1 PE=2 SV=1)

HSP 1 Score: 380.948 bits (977), Expect = 4.510e-107
Identity = 176/196 (89.80%), Postives = 190/196 (96.94%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPY+ RP+S+FNAPF+TTNSGAPVWNNNSSMT+GPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD++NL+CADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFF+RDGMKF   VH LKPNPKSHIQENWRI+DFFSHHPESL+MFSFLF+++G P
Sbjct:    1 MDPYRHRPSSAFNAPFWTTNSGAPVWNNNSSMTVGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDITNLSCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFIRDGMKFTRHVHPLKPNPKSHIQENWRILDFFSHHPESLNMFSFLFDDIGIP 196

HSP 2 Score: 29.261 bits (64), Expect = 4.510e-107
Identity = 11/14 (78.57%), Postives = 13/14 (92.86%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HM+GSGVNT
Sbjct:  196 PQDYRHMDGSGVNT 209
[Close]
BLAST of EY683491 vs. ExPASy Swiss-Prot
Match: CATA4_SOYBN (Catalase-4 OS=Glycine max GN=CAT4 PE=2 SV=1)

HSP 1 Score: 382.104 bits (980), Expect = 1.002e-106
Identity = 176/196 (89.80%), Postives = 191/196 (97.45%), Query Frame = 1 
Query:   88 MDPYKFRPASSFNAPFFTTNSGAPVWNNNSSMTIGPRGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDVSNLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNFPVFFVRDGMKFPDMVHALKPNPKSHIQENWRIVDFFSHHPESLHMFSFLFEEVGCP 675
            MDPYK RP+S+FN+PF+TTNSGAP+WNNNSS+T+G RGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHD+S+LTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNN PVFFVRDGMKFPDMVHALKPNPK+HIQENWRI+DFFSH PESLHMF+FLF+++G P
Sbjct:    1 MDPYKHRPSSAFNSPFWTTNSGAPIWNNNSSLTVGARGPILLEDYHLVEKLANFDRERIPERVVHARGASAKGFFEVTHDISHLTCADFLRAPGVQTPVIVRFSTVIHERGSPETLRDPRGFAVKFYTREGNFDLVGNNLPVFFVRDGMKFPDMVHALKPNPKNHIQENWRILDFFSHFPESLHMFTFLFDDLGVP 196

HSP 2 Score: 26.9498 bits (58), Expect = 1.002e-106
Identity = 10/14 (71.43%), Postives = 12/14 (85.71%), Query Frame = 3 
Query:  675 PQNYGHMEGSGVNT 716
            PQ+Y HM+G GVNT
Sbjct:  196 PQDYRHMDGFGVNT 209
The following BLAST results are available for this feature:
BLAST of EY683491 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 142
Match NameE-valueIdentityDescription
CATA2_ARATH2.853e-11293.37Catalase-2 OS=Arabidopsis thaliana GN=CAT2 PE=1 SV... [more]
CATA3_NICPL7.613e-11091.84Catalase isozyme 3 OS=Nicotiana plumbaginifolia GN... [more]
CATA_SOLAP2.206e-10991.33Catalase OS=Soldanella alpina PE=2 SV=1[more]
CATA2_GOSHI2.879e-10991.33Catalase isozyme 2 OS=Gossypium hirsutum GN=CAT2 P... [more]
CATA1_GOSHI2.879e-10990.82Catalase isozyme 1 OS=Gossypium hirsutum GN=CAT1 P... [more]
CATA3_SOYBN1.420e-10891.33Catalase-3 OS=Glycine max GN=CAT3 PE=2 SV=1[more]
CATA1_SOYBN1.420e-10891.33Catalase-1/2 OS=Glycine max GN=CAT1 PE=2 SV=1[more]
CATA_PHAAU2.030e-10790.82Catalase OS=Phaseolus aureus PE=2 SV=1[more]
CATA1_CUCPE4.510e-10789.80Catalase isozyme 1 OS=Cucurbita pepo GN=CAT1 PE=2 ... [more]
CATA4_SOYBN1.002e-10689.80Catalase-4 OS=Glycine max GN=CAT4 PE=2 SV=1[more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-650-050-H09-CT.F Sweet orange leaf, young greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY683491 ID=EY683491; Name=EY683491; organism=Citrus sinensis; type=EST; length=722bp
CCACACAATCCCCATTTGTCTACTCTCTCTTTTTCTTTCTCGTAGTGTAG
CTTCTCGTTCAATCTTTTTTTCTGCTTCCCTCCCGCTATGGATCCATACA
AGTTCCGCCCTGCCAGTTCTTTCAACGCCCCCTTCTTTACCACCAATTCC
GGCGCTCCGGTATGGAACAACAACTCATCAATGACGATTGGACCTAGAGG
TCCAATCCTCCTTGAGGATTATCATCTAGTGGAGAAACTTGCAAATTTTG
ATAGGGAGCGGATTCCAGAACGTGTTGTCCATGCCAGGGGAGCCAGTGCT
AAGGGTTTCTTTGAGGTCACCCATGATGTTTCTAACCTTACTTGTGCTGA
TTTCCTTCGAGCCCCAGGGGTTCAGACGCCTGTCATTGTCCGCTTCTCCA
CTGTTATCCATGAGCGTGGCAGCCCTGAAACCCTGAGAGATCCTCGAGGA
TTTGCGGTCAAGTTTTACACCAGAGAGGGTAATTTTGATCTTGTGGGAAA
CAATTTCCCCGTCTTTTTTGTCCGTGATGGAATGAAATTCCCTGACATGG
TCCATGCGCTTAAACCTAACCCAAAGTCTCACATCCAAGAGAACTGGAGG
ATTGTTGACTTCTTCTCCCATCATCCTGAAAGCTTGCACATGTTCTCCTT
CTTGTTTGAGGAGGTGGGGTGTCCCCACAAAATTACGGGCACATGGAAGG
ATCTGGTGTTAACACCTTACTG
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