EY708638

Overview
NameEY708638
Unique NameEY708638
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length906
Libraries
Library NameType
Sweet orange fruit, development stadium (2 of 6)cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD2_BACSU (6-phosphogluconate dehydrogenase, decarboxylating 2 OS=Bacillus subtilis GN=yqjI PE=1 SV=4)

HSP 1 Score: 124.02 bits (310), Expect = 6.498e-38
Identity = 59/102 (57.84%), Postives = 71/102 (69.61%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK+AYDR  +L NLL+D  F   +   Q A R+V+ LA+  G+  P  SS+LAY+DSYR   LPANL+QAQRDYFGAHTYER D EG FHTEW K
Sbjct:  368 RAAFLQKIKEAYDREPELDNLLLDSYFKNIVESYQGALRQVISLAVAQGVPVPSFSSALAYYDSYRTAVLPANLIQAQRDYFGAHTYERTDKEGIFHTEWMK 469

HSP 2 Score: 40.4318 bits (93), Expect = 6.498e-38
Identity = 19/39 (48.72%), Postives = 25/39 (64.10%), Query Frame = -3 
Query:  659 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGAVSL 775
            Y  G   ++A S +  WDLK+GE+A I+ GGCII A  L
Sbjct:  334 YAQGFAQMKAASEEYNWDLKYGEIAMIFRGGCIIRAAFL 372

HSP 3 Score: 35.4242 bits (80), Expect = 6.498e-38
Identity = 22/48 (45.83%), Postives = 32/48 (66.67%), Query Frame = -1 
Query:  763 LKKERVKLQNLKIRGLWVQSNQAVTRQKLIDEVRQALYASKICSTAQG 906
            +K+ERVK   L + G  V+      +++LI+ VR+AL+ SKICS AQG
Sbjct:  292 MKEERVKASGL-LSGPEVKP-VTENKEELIEAVRKALFMSKICSYAQG 337
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_SYNE7 (6-phosphogluconate dehydrogenase, decarboxylating OS=Synechococcus elongatus (strain PCC 7942) GN=gnd PE=1 SV=4)

HSP 1 Score: 137.502 bits (345), Expect = 3.931e-37
Identity = 64/101 (63.37%), Postives = 76/101 (75.25%), Query Frame = -2 
Query:  372 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWF 674
            R   L++IK+AYD +  LANLL+ PEF + I+DRQ AWRRV+ +A   GI  P  S+SL YFDSYRR+RLP NL QAQRDYFGAHTYER D  GSFH +WF
Sbjct:  371 RAGFLNKIKQAYDADPTLANLLLAPEFRQTILDRQLAWRRVIAIAAERGIPVPAFSASLDYFDSYRRDRLPQNLTQAQRDYFGAHTYERTDRSGSFHAQWF 471

HSP 2 Score: 39.2762 bits (90), Expect = 3.931e-37
Identity = 19/29 (65.52%), Postives = 20/29 (68.97%), Query Frame = -1 
Query:  745 RQKLIDEVRQALYASKICSTAQGMNLFVQ 831
            RQ  ID VR ALY SKICS AQGM L  +
Sbjct:  318 RQAFIDSVRDALYCSKICSYAQGMALLAK 346
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAES (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus epidermidis (strain ATCC 12228) GN=gnd PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 9.448e-36
Identity = 58/100 (58.00%), Postives = 73/100 (73.00%), Query Frame = -2 
Query:  375 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEW 674
            R   L +IK AYD N +L NLL+DP F   +++ Q A R VV  ++ +G+ TPG S+S+ Y+DSYR E LPANL+QAQRDYFGAHTYER D EG FHT+W
Sbjct:  366 RAQFLQKIKDAYDNNENLQNLLLDPYFKNIVMEYQDALREVVATSVYNGVPTPGFSASINYYDSYRSEDLPANLIQAQRDYFGAHTYERKDREGIFHTQW 465

HSP 2 Score: 36.1946 bits (82), Expect = 9.448e-36
Identity = 18/36 (50.00%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK GELA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGELAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.448e-36
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKTFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAEQ (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus epidermidis (strain ATCC 35984 / RP62A) GN=gnd PE=3 SV=1)

HSP 1 Score: 125.176 bits (313), Expect = 9.448e-36
Identity = 58/100 (58.00%), Postives = 73/100 (73.00%), Query Frame = -2 
Query:  375 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEW 674
            R   L +IK AYD N +L NLL+DP F   +++ Q A R VV  ++ +G+ TPG S+S+ Y+DSYR E LPANL+QAQRDYFGAHTYER D EG FHT+W
Sbjct:  366 RAQFLQKIKDAYDNNENLQNLLLDPYFKNIVMEYQDALREVVATSVYNGVPTPGFSASINYYDSYRSEDLPANLIQAQRDYFGAHTYERKDREGIFHTQW 465

HSP 2 Score: 36.1946 bits (82), Expect = 9.448e-36
Identity = 18/36 (50.00%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK GELA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGELAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.448e-36
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKTFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAW (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MW2) GN=gnd PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.978e-35
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK AYD N  L NLL+DP F   + + Q A R VV   + +G+ TPG SSS+ Y+DSYR   LPANL+QAQRDYFGAHTYER D EG FHT+W +
Sbjct:  366 RAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIE 467

HSP 2 Score: 35.039 bits (79), Expect = 9.978e-35
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK G+LA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.978e-35
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKDFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAS (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MSSA476) GN=gnd PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.978e-35
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK AYD N  L NLL+DP F   + + Q A R VV   + +G+ TPG SSS+ Y+DSYR   LPANL+QAQRDYFGAHTYER D EG FHT+W +
Sbjct:  366 RAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIE 467

HSP 2 Score: 35.039 bits (79), Expect = 9.978e-35
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK G+LA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.978e-35
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKDFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAR (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain MRSA252) GN=gnd PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.978e-35
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK AYD N  L NLL+DP F   + + Q A R VV   + +G+ TPG SSS+ Y+DSYR   LPANL+QAQRDYFGAHTYER D EG FHT+W +
Sbjct:  366 RAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIE 467

HSP 2 Score: 35.039 bits (79), Expect = 9.978e-35
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK G+LA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.978e-35
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKDFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAN (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain N315) GN=gnd PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.978e-35
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK AYD N  L NLL+DP F   + + Q A R VV   + +G+ TPG SSS+ Y+DSYR   LPANL+QAQRDYFGAHTYER D EG FHT+W +
Sbjct:  366 RAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIE 467

HSP 2 Score: 35.039 bits (79), Expect = 9.978e-35
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK G+LA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.978e-35
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKDFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAM (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain Mu50 / ATCC 700699) GN=gnd PE=1 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.978e-35
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK AYD N  L NLL+DP F   + + Q A R VV   + +G+ TPG SSS+ Y+DSYR   LPANL+QAQRDYFGAHTYER D EG FHT+W +
Sbjct:  366 RAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIE 467

HSP 2 Score: 35.039 bits (79), Expect = 9.978e-35
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK G+LA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.978e-35
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKDFLEKIRKALYMSKICSYAQG 335
[Close]
BLAST of EY708638 vs. ExPASy Swiss-Prot
Match: 6PGD_STAAC (6-phosphogluconate dehydrogenase, decarboxylating OS=Staphylococcus aureus (strain COL) GN=gnd PE=3 SV=1)

HSP 1 Score: 122.865 bits (307), Expect = 9.978e-35
Identity = 58/102 (56.86%), Postives = 70/102 (68.63%), Query Frame = -2 
Query:  369 RCCVLDRIKKAYDRNADLANLLVDPEFAKEIVDRQSAWRRVVCLAINSGISTPGMSSSLAYFDSYRRERLPANLVQAQRDYFGAHTYERIDMEGSFHTEWFK 674
            R   L +IK AYD N  L NLL+DP F   + + Q A R VV   + +G+ TPG SSS+ Y+DSYR   LPANL+QAQRDYFGAHTYER D EG FHT+W +
Sbjct:  366 RAQFLQKIKDAYDNNPGLQNLLLDPYFKNIVTEYQDALRDVVATGVQNGVPTPGFSSSINYYDSYRAADLPANLIQAQRDYFGAHTYERKDKEGVFHTQWIE 467

HSP 2 Score: 35.039 bits (79), Expect = 9.978e-35
Identity = 17/36 (47.22%), Postives = 20/36 (55.56%), Query Frame = -3 
Query:  668 YRTGDEFIRAKSMKKGWDLKWGELARIWEGGCIIGA 775
            Y  G   +R  S    W+LK G+LA IW  GCII A
Sbjct:  332 YAQGFAQMRKASEDNEWNLKLGDLAMIWREGCIIRA 367

HSP 3 Score: 31.187 bits (69), Expect = 9.978e-35
Identity = 12/23 (52.17%), Postives = 19/23 (82.61%), Query Frame = -1 
Query:  763 RQKLIDEVRQALYASKICSTAQG 831
            ++  ++++R+ALY SKICS AQG
Sbjct:  313 KKDFLEKIRKALYMSKICSYAQG 335
The following BLAST results are available for this feature:
BLAST of EY708638 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 54
Match NameE-valueIdentityDescription
6PGD2_BACSU6.498e-3857.846-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_SYNE73.931e-3763.376-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAES9.448e-3658.006-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAEQ9.448e-3658.006-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAW9.978e-3556.866-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAS9.978e-3556.866-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAR9.978e-3556.866-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAN9.978e-3556.866-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAM9.978e-3556.866-phosphogluconate dehydrogenase, decarboxylating ... [more]
6PGD_STAAC9.978e-3556.866-phosphogluconate dehydrogenase, decarboxylating ... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C3-701-085-D06-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY708638 ID=EY708638; Name=EY708638; organism=Citrus sinensis; type=EST; length=906bp
CCACGCGTCCGCAAAATATAAAACTTCTCTTTGATAATTTGATTAAACAT
TACATAAAAAGCCATTATGCCCAACAGTTATAAATACACGGCTCCTTAAA
CTGCCGGGAACAAAAACTTAATCAATTTGCACCTTAACACAAAGCAAAGC
AGAAGATCTGCCCAAGCACAAGCATGACAAACAAAAGAGAAATAATTCCA
GAGATTTGATCTGATGAAACAATTCATACTTAAAACACACTTTCACCAAA
CACCAGTGGTGTAAGCTCTGCCCAGCTAGTCTAAAGCAGAGATTATTATG
TTTATTAAGAGTTCTGGTTAAGCTGAATCTTTTTAGGAGAATAATTAGAT
TTTGGACTGTTTGGCAATCTTGAACCACTCGGTATGAAAAGATCCTTCCA
TATCAATCCTTTCATATGTATGAGCACCAAAATAATCTCGTTGAGCTTGG
ACCAAATTAGCTGGCAACCTTTCCCTTCTGTATGAGTCGAAATAAGCAAG
ACTGGATGACATACCAGGTGTGCTAATACCCGAGTTGATAGCAAGGCAAA
CAACTCTTCGCCAGGCAGACTGCCTATCAACAATTTCCTTTGCAAACTCA
GGGTCCACAAGAAGGTTTGCCAGATCAGCATTCCTATCGTATGCCTTCTT
GATTCTGTCAAGGACACAGCACCGATAATGCAACCCCCCTCCCAAATCCT
AGCCAGTTCCCCCCACTTCAAGTCCCATCCCTTTTTCATACTCTTTGCAC
GAATAAATTCATCCCCTGTGCGGTACTACATATCTTGGATGCATAAAGAG
CCTGCCTCACTTCATCAATCAACTTCTGCCTGGTCACAGCTTGGTTAGAC
TGAACCCAAAGGCCCCTGATTTTAAGATTTTGCAGCTTCACTCTTTCCTT
CTTTAA
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