EY744419 | Citrus Genome Database

Overview


Name	EY744419
Unique Name	EY744419
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	962

Libraries


Library Name	Type
Sweet orange flower, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 1.113e-31
Identity = 48/106 (45.28%), Postives = 65/106 (61.32%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYCY+  Q SP +       WPC     YYGRGPIQ+++  +Y   G+A+ VDL+N PDL+ATD V+S +++ W      SP+
Sbjct:  123 FLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGPIQISYNYNYGQAGKAIGVDLVNNPDLVATDAVISFKTAFWFWMTPQSPK 228

HSP 2 Score: 47.3654 bits (111), Expect = 1.113e-31
Identity = 19/49 (38.78%), Postives = 28/49 (57.14%), Query Frame = 1

Query:  133 CGERDTGHRTDGGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            CG       +  G++G++++R MF  +L   N+  C   GFYTYDAF A
Sbjct:   51 CGSGCQSQCSSTGDIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIA 99

HSP 3 Score: 29.261 bits (64), Expect = 1.113e-31
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V    W
Sbjct:  220 FWMTPQSPKPSCHNVITGGW 239

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI1_THECC (Endochitinase 1 OS=Theobroma cacao GN=CHIA1 PE=2 SV=1)

HSP 1 Score: 97.8265 bits (242), Expect = 2.437e-31
Identity = 46/94 (48.94%), Postives = 58/94 (61.70%), Query Frame = 2

Query:  305 GWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            GW  APDG Y WGYCY  +++PA      P +PC     Y+GRGP+QL W  +Y   G A+ VDLLN PDLLATDP +S +S+ W      SP+
Sbjct:  143 GWA-APDGPYTWGYCYNRELNPADYCQWDPNYPCAPGKQYFGRGPMQLTWNYNYGQCGRAIGVDLLNNPDLLATDPTISFKSAFWFWMTPQSPK 235

HSP 2 Score: 48.521 bits (114), Expect = 2.437e-31
Identity = 22/49 (44.90%), Postives = 29/49 (59.18%), Query Frame = 1

Query:  133 CGERDTGHRTDGGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            C  + +G   D G L  +++RE F+ +L   ND  C A GFYTYDAF A
Sbjct:   58 CQSQCSGSGGDTGGLDSLITRERFDQMLLHRNDGGCPARGFYTYDAFIA 106

HSP 3 Score: 31.5722 bits (70), Expect = 2.437e-31
Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V I +W
Sbjct:  227 FWMTPQSPKPSCHDVIIGAW 246

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI2_PEA (Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=2)

HSP 1 Score: 105.916 bits (263), Expect = 3.326e-30
Identity = 47/105 (44.76%), Postives = 67/105 (63.81%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYC++ + +P++       +PC     YYGRGPIQ++W  +Y   G A+ VDLLN PDL+ATDPV+S ++++W      SP+
Sbjct:  126 FLGQTSHETTGGWPTAPDGPYAWGYCFLREQNPSTYCQASSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFWMTPQSPK 230

HSP 2 Score: 38.891 bits (89), Expect = 3.326e-30
Identity = 16/38 (42.11%), Postives = 22/38 (57.89%), Query Frame = 1

Query:  166 GGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            GG L  ++S + F ++L   ND  C    FYTYDAF +
Sbjct:   65 GGTLSSLISGDTFNNMLKHRNDNACQGKPFYTYDAFLS 102

HSP 3 Score: 29.261 bits (64), Expect = 3.326e-30
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V    W
Sbjct:  222 FWMTPQSPKPSCHDVITGGW 241

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIA_SECCE (Basic endochitinase A OS=Secale cereale GN=rsca PE=1 SV=1)

HSP 1 Score: 97.4413 bits (241), Expect = 4.320e-30
Identity = 48/107 (44.86%), Postives = 65/107 (60.75%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHP--YWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG +AWGYC+  Q   A+S+Y  P   WPC     YYGRGPIQL+   +Y   G A+ VDLL  PDL+ATDP +S ++++W      +P+
Sbjct:  138 FLAQTSHETTGGWATAPDGAFAWGYCF-KQERGATSNYCTPSAQWPCAPGKSYYGRGPIQLSHNYNYGPAGRAIGVDLLRNPDLVATDPTVSFKTAMWFWMTAQAPK 243

HSP 2 Score: 49.2914 bits (116), Expect = 4.320e-30
Identity = 22/41 (53.66%), Postives = 27/41 (65.85%), Query Frame = 1

Query:  166 GGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFAQSG 288
            GG +  IVSR +F+ +L   ND  C A GFYTYDAF A +G
Sbjct:   77 GGGVSSIVSRALFDRMLLHRNDGACQAKGFYTYDAFVAAAG 117

HSP 3 Score: 26.9498 bits (58), Expect = 4.320e-30
Identity = 10/20 (50.00%), Postives = 11/20 (55.00%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMTA   KPS H V    W
Sbjct:  235 FWMTAQAPKPSSHAVITGQW 254

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI2_GOSHI (Endochitinase 2 (Fragment) OS=Gossypium hirsutum PE=2 SV=2)

HSP 1 Score: 96.6709 bits (239), Expect = 5.621e-30
Identity = 46/95 (48.42%), Postives = 62/95 (65.26%), Query Frame = 2

Query:  305 GWTDAPDGEYAWGYCYITQVSPASSHYGH-PYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            GW  APDG YAWGYCY  +++P SS+    P +PC     Y+GRGP+QL+W  +Y   G A+ VDLLN PDLL++DP +S +S+ W      SP+
Sbjct:  123 GWA-APDGPYAWGYCYNRELNPPSSYCASDPNYPCAPGKQYFGRGPMQLSWNYNYGQCGRAIGVDLLNNPDLLSSDPTISFKSAFWFWMTPQSPK 216

HSP 2 Score: 44.669 bits (104), Expect = 5.621e-30
Identity = 20/37 (54.05%), Postives = 23/37 (62.16%), Query Frame = 1

Query:  169 GELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            G L  ++SRE F  +L   ND  C A GFYTYDAF A
Sbjct:   50 GGLTNLISRETFNQMLLHRNDGACPARGFYTYDAFIA 86

HSP 3 Score: 31.9574 bits (71), Expect = 5.621e-30
Identity = 17/52 (32.69%), Postives = 23/52 (44.23%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSWKTLLPTEPTCRA---GFLNYSLNQEFDQGGGF 707
            +WMT    KPSCH V I +W          RA   G +   +N   + G G+
Sbjct:  208 FWMTPQSPKPSCHNVIIGAWSPSSSDRAAGRATGYGVITNIINGGLECGKGW 259

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI1_GOSHI (Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 7.282e-30
Identity = 46/95 (48.42%), Postives = 62/95 (65.26%), Query Frame = 2

Query:  305 GWTDAPDGEYAWGYCYITQVSPASSHYGH-PYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            GW  APDG YAWGYCY  +++P SS+    P +PC     Y+GRGP+QL+W  +Y   G A+ VDLLN PDLL++DP +S +S+ W      SP+
Sbjct:  145 GWA-APDGPYAWGYCYNRELNPPSSYCASDPNYPCAPGKQYFGRGPMQLSWNYNYGQCGRAIGVDLLNNPDLLSSDPTISFKSAFWFWMTPQSPK 238

HSP 2 Score: 44.669 bits (104), Expect = 7.282e-30
Identity = 20/37 (54.05%), Postives = 23/37 (62.16%), Query Frame = 1

Query:  169 GELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            G L  ++SRE F  +L   ND  C A GFYTYDAF A
Sbjct:   72 GGLTNLISRETFNQMLLHRNDGACPARGFYTYDAFIA 108

HSP 3 Score: 31.5722 bits (70), Expect = 7.282e-30
Identity = 11/20 (55.00%), Postives = 13/20 (65.00%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSW 620
            +WMT    KPSCH V I +W
Sbjct:  230 FWMTPQSPKPSCHNVIIGAW 249

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIX_PEA (Endochitinase OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 100.138 bits (248), Expect = 1.812e-29
Identity = 47/111 (42.34%), Postives = 67/111 (60.36%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYITQVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQAILPPS 604
            FL   + +  GGW  APDG YAWGYC++++ +    +     WPC     YYGRGPIQL    +Y + G+A+  DL+N PDLL+T+P +S +++IW      +PQA  P S
Sbjct:  138 FLAQTSHETTGGWPTAPDGPYAWGYCFVSEQNTQEVYCSPKDWPCAPGKKYYGRGPIQLTHNYNYGLAGQAIKEDLINNPDLLSTNPTVSFKTAIWF---WMTPQANKPSS 245

HSP 2 Score: 51.2174 bits (121), Expect = 1.812e-29
Identity = 21/40 (52.50%), Postives = 28/40 (70.00%), Query Frame = 1

Query:  160 TDGGELGKIVSREMFEHLLPDTNDARCAATGFYTYDAFFA 279
            T GG++G++V   +F+ +L   ND RCA  GFYTYDAF A
Sbjct:   75 TGGGDVGRLVPSSLFDQMLKYRNDGRCAGHGFYTYDAFIA 114

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHIC_SOLLC (Basic 30 kDa endochitinase OS=Solanum lycopersicum GN=CHI9 PE=1 SV=1)

HSP 1 Score: 100.908 bits (250), Expect = 2.171e-28
Identity = 50/106 (47.17%), Postives = 64/106 (60.38%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATDPV+S +S+IW      SP+
Sbjct:  131 FLAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSAIWFWMTPQSPK 236

HSP 2 Score: 37.7354 bits (86), Expect = 2.171e-28
Identity = 18/53 (33.96%), Postives = 27/53 (50.94%), Query Frame = 1

Query:  133 CGERDTGHRTDGGELGKIVSREMFEHLLPDTNDARC-AATGFYTYDAFFAQSG 288
            C  +  G     G+LG ++S  MF+ +L   ND  C     FY+Y+AF   +G
Sbjct:   58 CQSQCPGGPGPSGDLGGVISNSMFDQMLNHRNDNACQGKNNFYSYNAFVTAAG 110

HSP 3 Score: 29.261 bits (64), Expect = 2.171e-28
Identity = 10/21 (47.62%), Postives = 12/21 (57.14%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V    W+
Sbjct:  228 FWMTPQSPKPSCHDVITGRWQ 248

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI3_SOLTU (Endochitinase 3 (Fragment) OS=Solanum tuberosum GN=CHTB3 PE=2 SV=1)

HSP 1 Score: 98.2117 bits (243), Expect = 8.009e-28
Identity = 49/106 (46.23%), Postives = 63/106 (59.43%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            FL   + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATD V+S +S+IW      SP+
Sbjct:  127 FLAQTSHETTGGWPSAPDGPYAWGYCFLREQGSPGDYCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDSVISFKSAIWFWMTPQSPK 232

HSP 2 Score: 38.5058 bits (88), Expect = 8.009e-28
Identity = 18/53 (33.96%), Postives = 28/53 (52.83%), Query Frame = 1

Query:  133 CGERDTGHRTDGGELGKIVSREMFEHLLPDTNDARC-AATGFYTYDAFFAQSG 288
            C  +  G     G+LG ++S  MF+ +L   ND  C     FY+Y+AF + +G
Sbjct:   54 CQSQCPGGPGPSGDLGGVISNSMFDQMLNHRNDNACQGKNNFYSYNAFISAAG 106

HSP 3 Score: 29.261 bits (64), Expect = 8.009e-28
Identity = 10/21 (47.62%), Postives = 12/21 (57.14%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V    W+
Sbjct:  224 FWMTPQSPKPSCHDVITGRWQ 244

[Close]

BLAST of EY744419 vs. ExPASy Swiss-Prot
Match: CHI1_SOLTU (Endochitinase 1 (Fragment) OS=Solanum tuberosum GN=CHTB1 PE=2 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 8.009e-28
Identity = 48/106 (45.28%), Postives = 62/106 (58.49%), Query Frame = 2

Query:  272 FLPNLAMKLLGGWTDAPDGEYAWGYCYIT-QVSPASSHYGHPYWPCPLPGLYYGRGPIQLAWKCDYLVGGEALWVDLLNKPDLLATDPVLSSRSSIWLLDDCPSPQ 586
            F    + +  GGW  APDG YAWGYC++  Q SP         WPC     Y+GRGPIQ++   +Y   G A+ VDLLN PDL+ATD V+S +S+IW      SP+
Sbjct:  127 FFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDSVISFKSAIWFWMTPQSPK 232

HSP 2 Score: 40.4318 bits (93), Expect = 8.009e-28
Identity = 19/53 (35.85%), Postives = 29/53 (54.72%), Query Frame = 1

Query:  133 CGERDTGHRTDGGELGKIVSREMFEHLLPDTNDARCAATG-FYTYDAFFAQSG 288
            C  +  G     G+LG ++S  MF+ +L   ND  C   G FY+Y+AF + +G
Sbjct:   54 CQSQCPGGPGPSGDLGGVISNSMFDQMLNHRNDNACQGKGNFYSYNAFISAAG 106

HSP 3 Score: 29.261 bits (64), Expect = 8.009e-28
Identity = 10/21 (47.62%), Postives = 12/21 (57.14%), Query Frame = 3

Query:  561 YWMTAHPHKPSCHQVSIDSWK 623
            +WMT    KPSCH V    W+
Sbjct:  224 FWMTPQSPKPSCHDVITGRWQ 244

The following BLAST results are available for this feature:

BLAST of EY744419 vs. ExPASy Swiss-Prot

BLAST of EY744419 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 45


Match Name	E-value	Identity	Description
CHIB_VITVI	1.113e-31	45.28	Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE...	[more]
CHI1_THECC	2.437e-31	48.94	Endochitinase 1 OS=Theobroma cacao GN=CHIA1 PE=2 S...	[more]
CHI2_PEA	3.326e-30	44.76	Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=...	[more]
CHIA_SECCE	4.320e-30	44.86	Basic endochitinase A OS=Secale cereale GN=rsca PE...	[more]
CHI2_GOSHI	5.621e-30	48.42	Endochitinase 2 (Fragment) OS=Gossypium hirsutum P...	[more]
CHI1_GOSHI	7.282e-30	48.42	Endochitinase 1 OS=Gossypium hirsutum PE=3 SV=1	[more]
CHIX_PEA	1.812e-29	42.34	Endochitinase OS=Pisum sativum PE=2 SV=1	[more]
CHIC_SOLLC	2.171e-28	47.17	Basic 30 kDa endochitinase OS=Solanum lycopersicum...	[more]
CHI3_SOLTU	8.009e-28	46.23	Endochitinase 3 (Fragment) OS=Solanum tuberosum GN...	[more]
CHI1_SOLTU	8.009e-28	45.28	Endochitinase 1 (Fragment) OS=Solanum tuberosum GN...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C5-003-008-D08-CT.F Sweet orange flower, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY744419 ID=EY744419; Name=EY744419; organism=Citrus sinensis; type=EST; length=962bp
CCACGCGTCCGGATAATTGATGTCGTACCCCTTGCTTTCAATGTGAGGCT
CATTGGGTCCTTACTAATTCTCTCTCTAGATTTATCTTTTGTACTAAGAG
GTTCAGCACAGAATTGAGGAAGCGGCGTTGTGTGCGGGGAACGTGATACT
GGCCACAGGACGGATGGCGGTGAACTGGGCAAGATCGTCTCACGGGAAAT
GTTTGAACACTTGCTTCCGGATACAAATGATGCCCGATGCGCTGCCACAG
GCTTCTACACTTATGATGCTTTTTTTGCCCAATCTGGCCATGAAACTACT
TGGGGGATGGACCGATGCACCTGATGGAGAATATGCTTGGGGATATTGCT
ATATTACGCAAGTGAGCCCTGCGTCGTCCCACTACGGTCATCCTTACTGG
CCATGTCCCTTGCCAGGGCTATACTATGGCCGAGGTCCCATTCAACTCGC
TTGGAAGTGCGATTACTTAGTGGGCGGAGAAGCCTTGTGGGTGGACTTGT
TGAACAAACCAGACCTACTTGCCACTGATCCTGTACTATCATCTCGGTCA
TCCATCTGGTTACTGGATGACTGCCCATCCCCACAAGCCATCCTGCCACC
AAGTCTCTATTGACTCATGGAAAACCCTTCTCCCAACTGAACCAACCTGC
CGAGCCGGCTTCCTGAATTACAGTCTTAACCAGGAATTTGATCAAGGTGG
GGGATTCCTCTGGGTCGAACTCTGGAGAATGCACCCCACCTCCCACAATG
GGTGGGGCAACTTTCCCCTGTTGGGGCGCCGAGGCCTCGGAACTAGACCC
CGGTGGAATAGCTCCTAGACACTTGGCGGAGACCATAAGATGAGCGCCCG
CTGCACCAAGCCCCTACCAATTCCTCCAGGACAAGACCGCTCCCCCGCGC
CTGCCACTCCCCTTACCCCACCGTCAACAGAACACCCTCCTGCTTCCTCT
ACCACGCACATG