EY663124 | Citrus Genome Database

Overview


Name	EY663124
Unique Name	EY663124
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	928

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP2_ARATH (Glycine dehydrogenase [decarboxylating] 2, mitochondrial OS=Arabidopsis thaliana GN=At4g33010 PE=1 SV=2)

HSP 1 Score: 107.457 bits (267), Expect = 5.304e-31
Identity = 53/81 (65.43%), Postives = 62/81 (76.54%), Query Frame = 1

Query:  394 HAVYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            +AVYHGP GLK+IAQR HGL G F+LGL KL   EVQ L FFDTVK+KC+DAHAIA AA K  +  + VDS T+TA FD+T
Sbjct:  415 YAVYHGPAGLKSIAQRVHGLAGIFSLGLNKLGVAEVQELPFFDTVKIKCSDAHAIADAASKSEINLRVVDSTTITASFDET 495

HSP 2 Score: 37.7354 bits (86), Expect = 5.304e-31
Identity = 21/40 (52.50%), Postives = 24/40 (60.00%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTR 755
            TTLDD   LF      K VPF+A SLA E + +I S LTR
Sbjct:  496 TTLDDVDKLFKVFASGKPVPFTAESLAPEVQNSIPSSLTR 535

HSP 3 Score: 29.261 bits (64), Expect = 5.304e-31
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  746 INEESTYLTHPVFNNYPT 799
            +  ES YLTHP+FN Y T
Sbjct:  533 LTRESPYLTHPIFNMYHT 550

HSP 4 Score: 74.7146 bits (182), Expect = 8.323e-13
Identity = 34/45 (75.56%), Postives = 41/45 (91.11%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+G+S+DSSGK ALR+AM TREQHIR DKATSNICTAQ
Sbjct:  361 KRMMPGRIIGISVDSSGKQALRMAMQTREQHIRRDKATSNICTAQ 405

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP_FLATR (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Flaveria trinervia GN=GDCSPA PE=3 SV=1)

HSP 1 Score: 110.538 bits (275), Expect = 2.543e-30
Identity = 55/81 (67.90%), Postives = 63/81 (77.78%), Query Frame = 1

Query:  394 HAVYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            + VYHGPEGLKTIA+R HGL GTFA GLKKL TV+VQ L FFDTVKV CAD+ AIA  AYK +M  + VD NT+T  FD+T
Sbjct:  411 YGVYHGPEGLKTIAKRVHGLAGTFAAGLKKLGTVQVQDLPFFDTVKVTCADSKAIAEEAYKHKMNLRIVDKNTITVAFDET 491

HSP 2 Score: 34.2686 bits (77), Expect = 2.543e-30
Identity = 19/40 (47.50%), Postives = 24/40 (60.00%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTR 755
            TT++D   LF      K V F+AAS+A E + AI SGL R
Sbjct:  492 TTIEDVDTLFKVFALGKPVTFTAASIAPEVQDAIPSGLVR 531

HSP 3 Score: 27.335 bits (59), Expect = 2.543e-30
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2

Query:  755 ESTYLTHPVFNNYPT 799
            E+ YLTHP+FN Y T
Sbjct:  532 ETPYLTHPIFNMYHT 546

HSP 4 Score: 78.1814 bits (191), Expect = 7.528e-14
Identity = 36/45 (80.00%), Postives = 42/45 (93.33%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+GVS+DSSGKPALR+AM TREQHIR DKATSNICTAQ
Sbjct:  357 KRMMPGRIIGVSVDSSGKPALRMAMQTREQHIRRDKATSNICTAQ 401

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP_FLAAN (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Flaveria anomala GN=GDCSP PE=3 SV=1)

HSP 1 Score: 107.071 bits (266), Expect = 3.303e-30
Identity = 54/81 (66.67%), Postives = 62/81 (76.54%), Query Frame = 1

Query:  394 HAVYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            + VYHGPEGLKTIA+R HGL GTFA GLKKL TV+VQ L FFDTVKV CAD+ AIA  A K +M  + VD NT+T  FD+T
Sbjct:  411 YGVYHGPEGLKTIAKRVHGLAGTFAAGLKKLGTVQVQDLPFFDTVKVTCADSKAIAEEACKHKMNLRIVDKNTITVAFDET 491

HSP 2 Score: 37.3502 bits (85), Expect = 3.303e-30
Identity = 20/40 (50.00%), Postives = 25/40 (62.50%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTR 755
            TT++D   LF      K VPF+AAS+A E + AI SGL R
Sbjct:  492 TTIEDVDTLFKVFALGKPVPFTAASIAPEVQDAIPSGLVR 531

HSP 3 Score: 27.335 bits (59), Expect = 3.303e-30
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2

Query:  755 ESTYLTHPVFNNYPT 799
            E+ YLTHP+FN Y T
Sbjct:  532 ETPYLTHPIFNMYHT 546

HSP 4 Score: 78.1814 bits (191), Expect = 7.528e-14
Identity = 36/45 (80.00%), Postives = 42/45 (93.33%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+GVS+DSSGKPALR+AM TREQHIR DKATSNICTAQ
Sbjct:  357 KRMMPGRIIGVSVDSSGKPALRMAMQTREQHIRRDKATSNICTAQ 401

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSPB_FLAPR (Glycine dehydrogenase [decarboxylating] B, mitochondrial OS=Flaveria pringlei GN=GDCSPB PE=3 SV=1)

HSP 1 Score: 109.383 bits (272), Expect = 5.568e-30
Identity = 55/79 (69.62%), Postives = 62/79 (78.48%), Query Frame = 1

Query:  400 VYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            VYHGPEGLKTIA+R HGL GTFA GLKKL TV+VQ L FFDTVKV CAD+ AIA  AYK +M  + VD NT+T  FD+T
Sbjct:  413 VYHGPEGLKTIAKRVHGLAGTFASGLKKLGTVQVQDLPFFDTVKVTCADSKAIAEEAYKHKMNLRIVDKNTITVAFDET 491

HSP 2 Score: 34.2686 bits (77), Expect = 5.568e-30
Identity = 19/40 (47.50%), Postives = 24/40 (60.00%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTR 755
            TT++D   LF      K V F+AAS+A E + AI SGL R
Sbjct:  492 TTIEDVDTLFKVFALGKPVTFTAASIAPEVQDAIPSGLVR 531

HSP 3 Score: 27.335 bits (59), Expect = 5.568e-30
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2

Query:  755 ESTYLTHPVFNNYPT 799
            E+ YLTHP+FN Y T
Sbjct:  532 ETPYLTHPIFNMYHT 546

HSP 4 Score: 78.1814 bits (191), Expect = 7.528e-14
Identity = 36/45 (80.00%), Postives = 42/45 (93.33%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+GVS+DSSGKPALR+AM TREQHIR DKATSNICTAQ
Sbjct:  357 KRMMPGRIIGVSVDSSGKPALRMAMQTREQHIRRDKATSNICTAQ 401

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSPA_FLAPR (Glycine dehydrogenase [decarboxylating] A, mitochondrial OS=Flaveria pringlei GN=GDCSPA PE=2 SV=1)

HSP 1 Score: 108.227 bits (269), Expect = 1.219e-29
Identity = 54/79 (68.35%), Postives = 61/79 (77.22%), Query Frame = 1

Query:  400 VYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            VYHGPEGLKTIA+R HGL GTFA GLKKL TV+VQ L FFDTVKV C D+ AIA  AYK +M  + VD NT+T  FD+T
Sbjct:  416 VYHGPEGLKTIAKRVHGLAGTFAAGLKKLGTVQVQDLPFFDTVKVTCVDSKAIAEEAYKHKMNLRIVDKNTITVAFDET 494

HSP 2 Score: 34.2686 bits (77), Expect = 1.219e-29
Identity = 19/40 (47.50%), Postives = 24/40 (60.00%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTR 755
            TT++D   LF      K V F+AAS+A E + AI SGL R
Sbjct:  495 TTIEDVDTLFKVFALGKPVTFTAASIAPEVQDAIPSGLVR 534

HSP 3 Score: 27.335 bits (59), Expect = 1.219e-29
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = 2

Query:  755 ESTYLTHPVFNNYPT 799
            E+ YLTHP+FN Y T
Sbjct:  535 ETPYLTHPIFNMYHT 549

HSP 4 Score: 78.1814 bits (191), Expect = 7.528e-14
Identity = 36/45 (80.00%), Postives = 42/45 (93.33%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+GVS+DSSGKPALR+AM TREQHIR DKATSNICTAQ
Sbjct:  360 KRMMPGRIIGVSVDSSGKPALRMAMQTREQHIRRDKATSNICTAQ 404

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP_SOLTU (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Solanum tuberosum GN=GDCSP PE=2 SV=1)

HSP 1 Score: 108.612 bits (270), Expect = 2.378e-29
Identity = 55/81 (67.90%), Postives = 63/81 (77.78%), Query Frame = 1

Query:  394 HAVYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            +AVYHGPEGLKTI QR HGL GTF+ GLKKL TVEVQ L FFDTVKVKC+DA AIA  A K  +  + VD+NT+T  FD+T
Sbjct:  412 YAVYHGPEGLKTIGQRVHGLAGTFSAGLKKLGTVEVQDLPFFDTVKVKCSDAKAIADVANKNDINLRIVDNNTITVSFDET 492

HSP 2 Score: 40.0466 bits (92), Expect = 2.378e-29
Identity = 29/75 (38.67%), Postives = 37/75 (49.33%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTRRVRI*HILFLTTTPRA*VAQMHQLXLSYDSPYALV 860
            TTL+D   LF      K VPF+A S+A+E E  I SGLTR        FLT          HQ+  SY + + L+
Sbjct:  493 TTLEDVDDLFKVFALGKPVPFTAQSIAQEVENLIPSGLTRETP-----FLT----------HQIFNSYHTEHELL 552

HSP 3 Score: 75.8702 bits (185), Expect = 3.736e-13
Identity = 34/45 (75.56%), Postives = 42/45 (93.33%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+G+S+DS+GKPALR+AM TREQHIR DKATSNICTAQ
Sbjct:  358 KRMMPGRIIGLSVDSTGKPALRMAMQTREQHIRRDKATSNICTAQ 402

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP1_ARATH (Glycine dehydrogenase [decarboxylating] 1, mitochondrial OS=Arabidopsis thaliana GN=GDCSP PE=1 SV=1)

HSP 1 Score: 103.605 bits (257), Expect = 4.496e-29
Identity = 53/81 (65.43%), Postives = 61/81 (75.31%), Query Frame = 1

Query:  394 HAVYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            +AVYHGPEGLK+IAQR HGL G FALGLKKL T +VQ L FFDTVKV C+DA AI   A K  +  + VDSNT+T  FD+T
Sbjct:  421 YAVYHGPEGLKSIAQRVHGLAGVFALGLKKLGTAQVQDLPFFDTVKVTCSDATAIFDVAAKKEINLRLVDSNTITVAFDET 501

HSP 2 Score: 35.039 bits (79), Expect = 4.496e-29
Identity = 21/40 (52.50%), Postives = 22/40 (55.00%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTR 755
            TTLDD   LF      K V F+A SLA E   AI S LTR
Sbjct:  502 TTLDDVDKLFEVFASGKPVQFTAESLAPEFNNAIPSSLTR 541

HSP 3 Score: 29.261 bits (64), Expect = 4.496e-29
Identity = 11/18 (61.11%), Postives = 13/18 (72.22%), Query Frame = 2

Query:  746 INEESTYLTHPVFNNYPT 799
            +  ES YLTHP+FN Y T
Sbjct:  539 LTRESPYLTHPIFNMYHT 556

HSP 4 Score: 75.0998 bits (183), Expect = 6.373e-13
Identity = 35/45 (77.78%), Postives = 41/45 (91.11%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+GVS+DSSGK ALR+AM TREQHIR DKATSNICTAQ
Sbjct:  367 KRMMPGRIIGVSVDSSGKQALRMAMQTREQHIRRDKATSNICTAQ 411

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP_PEA (Glycine dehydrogenase [decarboxylating], mitochondrial OS=Pisum sativum GN=GDCSP PE=1 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 4.981e-26
Identity = 54/81 (66.67%), Postives = 62/81 (76.54%), Query Frame = 1

Query:  394 HAVYHGPEGLKTIAQRGHGLPGTFALGLKKLVTVEVQRLHFFDTVKVKCADAHAIASAAYKIRMKSQDVDSNTVTAFFDDT 636
            +AVYHGPEGLK IAQR HGL G FALGLKKL  +EVQ L FFDTVKVK ++A AIA AA K  +  + VD NT+TA FD+T
Sbjct:  434 YAVYHGPEGLKAIAQRVHGLAGVFALGLKKL-GLEVQDLGFFDTVKVKTSNAKAIADAAIKSEINLRVVDGNTITAAFDET 513

HSP 2 Score: 36.965 bits (84), Expect = 4.981e-26
Identity = 34/90 (37.78%), Postives = 43/90 (47.78%), Query Frame = 3

Query:  636 TTLDDAR*LFISLTRSKSVPFSAASLAEEGETAISSGLTRR-VRI*HILFLT-TTPRA*VAQMHQLXLSYDSPYALVYSLGVLVHGLNAT 899
            TTL+D   LF      K V F+AASLA E + AI SGL R    + H +F T  T    +  +H+L     S    +  LG     LNAT
Sbjct:  514 TTLEDVDKLFKVFAGGKPVSFTAASLAPEFQNAIPSGLVRESPYLTHPIFNTYQTEHELLRYIHRLQSKDLSLCHSMIPLGSCTMKLNAT 603

HSP 3 Score: 75.0998 bits (183), Expect = 6.373e-13
Identity = 35/45 (77.78%), Postives = 41/45 (91.11%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +RM+P +I+GVS+DSSGK ALR+AM TREQHIR DKATSNICTAQ
Sbjct:  380 KRMMPGRIIGVSVDSSGKQALRMAMQTREQHIRRDKATSNICTAQ 424

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP_NOSP7 (Glycine dehydrogenase [decarboxylating] OS=Nostoc punctiforme (strain ATCC 29133 / PCC 73102) GN=gcvP PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.395e-12
Identity = 33/46 (71.74%), Postives = 41/46 (89.13%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQV 148
            +R++P +IVGVS D+ GKPALR+A+ TREQHIR +KATSNICTAQV
Sbjct:  305 KRLVPGRIVGVSKDAQGKPALRLALQTREQHIRREKATSNICTAQV 350

[Close]

BLAST of EY663124 vs. ExPASy Swiss-Prot
Match: GCSP2_COLP3 (Glycine dehydrogenase [decarboxylating] 2 OS=Colwellia psychrerythraea (strain 34H / ATCC BAA-681) GN=gcvP2 PE=3 SV=1)

HSP 1 Score: 72.0182 bits (175), Expect = 5.395e-12
Identity = 33/45 (73.33%), Postives = 39/45 (86.67%), Query Frame = 2

Query:   11 ERMIPSKIVGVSIDSSGKPALRVAMLTREQHIRMDKATSNICTAQ 145
            +R IP +++GVSIDS GKPALR+AM TREQHIR +KA SNICTAQ
Sbjct:  294 KRTIPGRVIGVSIDSKGKPALRMAMQTREQHIRREKANSNICTAQ 338

The following BLAST results are available for this feature:

BLAST of EY663124 vs. ExPASy Swiss-Prot

BLAST of EY663124 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 71


Match Name	E-value	Identity	Description
GCSP2_ARATH	5.304e-31	65.43	Glycine dehydrogenase [decarboxylating] 2, mitocho...	[more]
GCSP_FLATR	2.543e-30	67.90	Glycine dehydrogenase [decarboxylating], mitochond...	[more]
GCSP_FLAAN	3.303e-30	66.67	Glycine dehydrogenase [decarboxylating], mitochond...	[more]
GCSPB_FLAPR	5.568e-30	69.62	Glycine dehydrogenase [decarboxylating] B, mitocho...	[more]
GCSPA_FLAPR	1.219e-29	68.35	Glycine dehydrogenase [decarboxylating] A, mitocho...	[more]
GCSP_SOLTU	2.378e-29	67.90	Glycine dehydrogenase [decarboxylating], mitochond...	[more]
GCSP1_ARATH	4.496e-29	65.43	Glycine dehydrogenase [decarboxylating] 1, mitocho...	[more]
GCSP_PEA	4.981e-26	66.67	Glycine dehydrogenase [decarboxylating], mitochond...	[more]
GCSP_NOSP7	5.395e-12	71.74	Glycine dehydrogenase [decarboxylating] OS=Nostoc ...	[more]
GCSP2_COLP3	5.395e-12	73.33	Glycine dehydrogenase [decarboxylating] 2 OS=Colwe...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-101-051-G04-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY663124 ID=EY663124; Name=EY663124; organism=Citrus sinensis; type=EST; length=928bp
CCACGCGTCCGAGAGGATGATTCCCTCGAAGATTGTTGGTGTTAGTATTG
ATTCTTCAGGGAAGCCTGCCCTGCGTGTGGCTATGCTGACTAGGGAGCAG
CATATCCGCATGGACAAGGCTACCAGCAACATCTGCACCGCTCAAGTAAC
ATATTATTGTTATTCCTGATCTGTTTCTGGTTTACTTTGGAGGACTGCCT
GTGTAGAGATTGAAAAACTGCTTTCTAATTTTTTGAAAGTTTGAAGACTG
CACAACCTGTCTAGCTCAACAATTTTGTCATAAACACATGCATTCATATT
TTCTGCACCATAAAACATGCCCAATGAACTGACTTTGCTTTGGGTGAATA
AAACTTATCTATGTAGGCATTACTTGCAAACATGGCTGCTATGCATGCTG
TTTATCACGGACCTGAAGGCCTTAAAACCATTGCTCAAAGAGGCCATGGA
CTACCCGGGACTTTCGCCCTTGGGTTAAAAAAGCTGGTGACAGTGGAAGT
CCAGAGGCTTCACTTCTTTGACACTGTGAAGGTGAAGTGTGCGGATGCAC
ATGCCATTGCCAGTGCTGCTTACAAGATTCGAATGAAATCTCAGGATGTA
GATTCCAACACCGTCACTGCTTTCTTTGATGACACACCACCTTGGACGAT
GCTCGATAGCTTTTCATAAGTCTCACGAGGAGCAAATCAGTTCCATTCTC
TGCTGCATCTCTGGCTGAAGAGGGTGAGACTGCAATTTCGTCTGGATTAA
CGAGGAGAGTACGTATCTGACACATCCTGTTTTTAACAACTACCCCACGA
GCGTGAGTTGCTCAGATGCATCAACTGNTGCTATCATATGACTCTCCCTA
TGCCTTAGTATACTCCCTTGGGGTCTTGGTACATGGACTGAATGCAACAC
CGAATTGATACCATGACATGCCTACCTT