EY658843

Overview
NameEY658843
Unique NameEY658843
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length957
Libraries
Library NameType
Sweet orange leaf, greenhouse plantcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP_MEDSA (Malate dehydrogenase [NADP], chloroplastic OS=Medicago sativa GN=MDH1 PE=2 SV=1)

HSP 1 Score: 95.1301 bits (235), Expect = 9.949e-27
Identity = 48/65 (73.85%), Postives = 58/65 (89.23%), Query Frame = 3 
Query:  345 VFSVSHNDLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEKVLK 539
            VF +++ DLKAEEETKSWKK++ IAVSGAAGMI+NHLLFKLA+GEV  P+ PIALKLLGSE+ L+
Sbjct:   75 VFCLTY-DLKAEEETKSWKKLITIAVSGAAGMISNHLLFKLASGEVFGPNQPIALKLLGSERSLQ 138

HSP 2 Score: 46.9802 bits (110), Expect = 9.949e-27
Identity = 27/35 (77.14%), Postives = 28/35 (80.00%), Query Frame = 2 
Query:  263 ISCSV--NQVQAPVAVEQDDPKSKTNDCYGVFCLT 361
            ISCSV  NQVQAP AV+  DPKSK  DCYGVFCLT
Sbjct:   47 ISCSVAPNQVQAP-AVQTQDPKSKP-DCYGVFCLT 79
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP_PEA (Malate dehydrogenase [NADP], chloroplastic OS=Pisum sativum PE=1 SV=2)

HSP 1 Score: 98.5969 bits (244), Expect = 1.686e-26
Identity = 49/65 (75.38%), Postives = 59/65 (90.77%), Query Frame = 3 
Query:  345 VFSVSHNDLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEKVLK 539
            VF +++ DLKAEEETKSWKK++NIAVSGAAGMI+NHLLFKLA+GEV  PD PIALKLLGSE+ ++
Sbjct:   79 VFCLTY-DLKAEEETKSWKKLINIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSERSIQ 142

HSP 2 Score: 42.743 bits (99), Expect = 1.686e-26
Identity = 25/35 (71.43%), Postives = 25/35 (71.43%), Query Frame = 2 
Query:  263 ISCSV--NQVQAPVAVEQDDPKSKTNDCYGVFCLT 361
            ISCSV  NQVQ P A  QD PK K  DCYGVFCLT
Sbjct:   51 ISCSVAPNQVQVPAAQTQD-PKGKP-DCYGVFCLT 83
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP_MESCR (Malate dehydrogenase [NADP], chloroplastic OS=Mesembryanthemum crystallinum GN=MDH1 PE=2 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 1.487e-24
Identity = 46/55 (83.64%), Postives = 51/55 (92.73%), Query Frame = 3 
Query:  366 DLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEK 530
            DLKAEEETK+WKKM+ IAVSGAAGMI+NHLLFKLA+GEV  PD PIALKLLGSE+
Sbjct:   84 DLKAEEETKTWKKMITIAVSGAAGMISNHLLFKLASGEVFGPDQPIALKLLGSER 138

HSP 2 Score: 39.2762 bits (90), Expect = 1.487e-24
Identity = 21/35 (60.00%), Postives = 24/35 (68.57%), Query Frame = 2 
Query:  263 ISCSV--NQVQAPVAVEQDDPKSKTNDCYGVFCLT 361
            I CSV  NQVQAPVAV  +    K  +CYG+FCLT
Sbjct:   49 IRCSVAPNQVQAPVAVPAEGQTGKP-ECYGIFCLT 82
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP_FLABI (Malate dehydrogenase [NADP], chloroplastic OS=Flaveria bidentis PE=1 SV=1)

HSP 1 Score: 96.2857 bits (238), Expect = 2.878e-22
Identity = 47/62 (75.81%), Postives = 57/62 (91.94%), Query Frame = 3 
Query:  345 VFSVSHNDLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEK 530
            VF +++ DLKAEEETKSWKK++N+AVSGAAGMI+NHLLFKLA+GEV  PD PI+LKLLGSE+
Sbjct:   91 VFCLTY-DLKAEEETKSWKKIINVAVSGAAGMISNHLLFKLASGEVFGPDQPISLKLLGSER 151

HSP 2 Score: 30.8018 bits (68), Expect = 2.878e-22
Identity = 16/37 (43.24%), Postives = 23/37 (62.16%), Query Frame = 2 
Query:  260 TISCSV---NQVQAPVAVEQDDPKSKTNDCYGVFCLT 361
            +I CSV   +Q+QAP+      P  +  +C+GVFCLT
Sbjct:   65 SIRCSVTSSDQIQAPL------PAKQKPECFGVFCLT 95
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP_SPIOL (Malate dehydrogenase [NADP], chloroplastic OS=Spinacia oleracea GN=MDH PE=1 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 8.804e-21
Identity = 43/55 (78.18%), Postives = 48/55 (87.27%), Query Frame = 3 
Query:  366 DLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEK 530
            DLK EEET+SWKKM+ IA+SGAAG I+NHLLFKLA+G V  PD PIALKLLGSEK
Sbjct:   78 DLKKEEETRSWKKMITIAISGAAGTISNHLLFKLASGVVFGPDQPIALKLLGSEK 132

HSP 2 Score: 32.7278 bits (73), Expect = 8.804e-21
Identity = 19/35 (54.29%), Postives = 21/35 (60.00%), Query Frame = 2 
Query:  263 ISCSV--NQVQAPVAVEQDDPKSKTNDCYGVFCLT 361
            I CS+  NQVQ PVAV       K  +CYGVFC T
Sbjct:   43 ICCSLAPNQVQTPVAVPTGAQSIKP-ECYGVFCWT 76
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP1_SORBI (Malate dehydrogenase [NADP] 1, chloroplastic OS=Sorghum bicolor PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 1.490e-20
Identity = 45/55 (81.82%), Postives = 51/55 (92.73%), Query Frame = 3 
Query:  366 DLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEK 530
            DLKAE++TKSWKK+VNIAVSGAAGMI+NHLLFKLA+GEV   D PIALKLLGSE+
Sbjct:   73 DLKAEDKTKSWKKLVNIAVSGAAGMISNHLLFKLASGEVFGQDQPIALKLLGSER 127

HSP 2 Score: 28.8758 bits (63), Expect = 1.490e-20
Identity = 17/37 (45.95%), Postives = 21/37 (56.76%), Query Frame = 2 
Query:  260 TISCSVN---QVQAPVAVEQDDPKSKTNDCYGVFCLT 361
            T+ CSV+   QVQ  VA  +     K  DC+GVFC T
Sbjct:   37 TVRCSVDAAKQVQDGVATAEAPATRK--DCFGVFCTT 71
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP2_SORBI (Malate dehydrogenase [NADP] 2, chloroplastic OS=Sorghum bicolor PE=1 SV=1)

HSP 1 Score: 90.1225 bits (222), Expect = 7.213e-20
Identity = 44/55 (80.00%), Postives = 50/55 (90.91%), Query Frame = 3 
Query:  366 DLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEK 530
            DLKAE++TKSWKK+V IAVSGAAGMI+NHLLFKLA+GEV   D PIALKLLGSE+
Sbjct:   76 DLKAEDKTKSWKKLVTIAVSGAAGMISNHLLFKLASGEVFGQDQPIALKLLGSER 130

HSP 2 Score: 28.8758 bits (63), Expect = 7.213e-20
Identity = 15/36 (41.67%), Postives = 19/36 (52.78%), Query Frame = 2 
Query:  260 TISCSV----NQVQAPVAVEQDDPKSKTNDCYGVFC 355
            T+ CSV     QVQ  VA       +  N+C+GVFC
Sbjct:   37 TVRCSVVDAAKQVQDGVATAVGGGAASGNECFGVFC 72
[Close]
BLAST of EY658843 vs. ExPASy Swiss-Prot
Match: MDHP_MAIZE (Malate dehydrogenase [NADP], chloroplastic OS=Zea mays PE=1 SV=1)

HSP 1 Score: 90.8929 bits (224), Expect = 2.064e-19
Identity = 43/55 (78.18%), Postives = 51/55 (92.73%), Query Frame = 3 
Query:  366 DLKAEEETKSWKKMVNIAVSGAAGMIANHLLFKLAAGEVLRPDPPIALKLLGSEK 530
            DLKAE++TKSW+K+VN+AVSGAAGMI+NHLLFKLA+GEV   D PIALKLLGSE+
Sbjct:   76 DLKAEDKTKSWRKLVNVAVSGAAGMISNHLLFKLASGEVFGQDQPIALKLLGSER 130

HSP 2 Score: 26.5646 bits (57), Expect = 2.064e-19
Identity = 14/39 (35.90%), Postives = 21/39 (53.85%), Query Frame = 2 
Query:  260 TISCSVNQVQ-----APVAVEQDDPKSKTNDCYGVFCLT 361
            T+ CSV+  +        AV  + P S+  +C+GVFC T
Sbjct:   37 TVRCSVDATKQAQDGVATAVATEAPASR-KECFGVFCTT 74
The following BLAST results are available for this feature:
BLAST of EY658843 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 8
Match NameE-valueIdentityDescription
MDHP_MEDSA9.949e-2773.85Malate dehydrogenase [NADP], chloroplastic OS=Medi... [more]
MDHP_PEA1.686e-2675.38Malate dehydrogenase [NADP], chloroplastic OS=Pisu... [more]
MDHP_MESCR1.487e-2483.64Malate dehydrogenase [NADP], chloroplastic OS=Mese... [more]
MDHP_FLABI2.878e-2275.81Malate dehydrogenase [NADP], chloroplastic OS=Flav... [more]
MDHP_SPIOL8.804e-2178.18Malate dehydrogenase [NADP], chloroplastic OS=Spin... [more]
MDHP1_SORBI1.490e-2081.82Malate dehydrogenase [NADP] 1, chloroplastic OS=So... [more]
MDHP2_SORBI7.213e-2080.00Malate dehydrogenase [NADP] 2, chloroplastic OS=So... [more]
MDHP_MAIZE2.064e-1978.18Malate dehydrogenase [NADP], chloroplastic OS=Zea ... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C1-100-127-A11-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY658843 ID=EY658843; Name=EY658843; organism=Citrus sinensis; type=EST; length=957bp
CATAAGCCTTCCGAATCTTTCTCTCAAGACAAGACTTTTTAGTTTTAAAT
CTTTTCTGTTCACCTGCTGTTTCTTTTTCTCGAAATACTTTCTTTATATA
CCTCTGCTGGCTGCTGACATGGCGGTGGTGGCACAGCTCTCACCATCTTC
ATACACCGAAACCACCCGTCTCAGCTCTTCACAGCTCTCACTCTCATCGA
CCCACCTTTCATCTCTCCGTCGCCGTGCTTTTCGACCCATCATCCGACCC
AGAAACCCCACCATCTCTTGCTCTGTAAATCAAGTTCAAGCCCCGGTCGC
CGTTGAACAAGATGATCCTAAGAGCAAGACTAACGATTGCTATGGTGTTT
TCTGTCTCACATAATGATCTCAAAGCTGAAGAGGAGACAAAATCCTGGAA
GAAAATGGTTAATATTGCTGTGTCGGGTGCTGCTGGGATGATTGCTAATC
ATTTACTTTTCAAACTCGCAGCTGGGGAGGTTTTAAGGCCAGATCCACCG
ATTGCATTGAAACTACTGGGATCTGAAAAGGTCCTTAAAGGGCCCCTTTG
AAGGAGTGGGCGATGGAAATTTGAGGATTTCCTTTGTTTCCTTTGTTGAA
GGGGAGGGGAAAATCGGAAATAGATCCCAAATAAAGCTGTTTTAAAAAAG
CCAAATGGGCCCTTTTCTGATTGGAAAAAAAAACCACCATGGGGCCAGAA
AAGGGAAAAAACCCGGCTTTATTTAAAATTACATGGGGCCCAAATATTTG
GGAGAAACAAGGGAAAAAACTTTTTCAAAGGCCTGTTACCATACCCCGAA
ATGGGCCAAAAGGTAAAAAATTATTGGGGGGAAAACCCCATGATAACACC
ACAAAGGTCCATTTAATATTTGTTTTAGATAAAAGAGGTCCCGCACCCAC
AAAAACCTCCCGCAAAAAAAAAAACTATTTTAATGGGCTTTTTTAAAAAC
AAAAAAA
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