EY675446

Overview
NameEY675446
Unique NameEY675446
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1015
Libraries
Library NameType
Sweet orange leaf, infected with CiLVcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH6_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 6 OS=Arabidopsis thaliana GN=XTH6 PE=2 SV=2)

HSP 1 Score: 317.39 bits (812), Expect = 8.412e-86
Identity = 153/209 (73.21%), Postives = 177/209 (84.69%), Query Frame = 2
Query:    2 PATFLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKN--SGRAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTFEY*RGGPVPGPT 622
            PATF++DFK  WS++H+RQ+E G+AIQLVLDQ++GCGFASKR+YLFGRVSMKIKL+PGDSAGTVTAFYMNS+T  VRDELDFEFLGNR+GQPY+VQTNI+A+GKGDREQRVNLWFDP+ DYH YTILW+H HIVFYVDDVPIR YKN  +    +P +QPMGVYSTLWEADDWATRG   EK + +K  F    K F+   G PVPGPT
Sbjct:   32 PATFVEDFKAAWSESHIRQMEDGKAIQLVLDQSTGCGFASKRKYLFGRVSMKIKLIPGDSAGTVTAFYMNSDTATVRDELDFEFLGNRSGQPYSVQTNIFAHGKGDREQRVNLWFDPSMDYHTYTILWSHKHIVFYVDDVPIREYKNNEAKNIAYPTSQPMGVYSTLWEADDWATRG-GLEKIDWSKAPFYAYYKDFDI-EGCPVPGPT 238          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH7_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 7 OS=Arabidopsis thaliana GN=XTH7 PE=2 SV=2)

HSP 1 Score: 310.842 bits (795), Expect = 7.873e-84
Identity = 150/210 (71.43%), Postives = 174/210 (82.86%), Query Frame = 2
Query:    2 PATFLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKN--SGRAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTFEY*RGGPVPGPTN 625
            PA F DDF++ WSD H+ QI+GGRAIQL LD +SGCGFASK+QYLFGRVSMKIKL+PGDSAGTVTAFYMNS+T++VRDELDFEFLGNR+GQPYTVQTN++A+GKGDREQRVNLWFDP+ D+H Y I WNH  IVFYVD+VPIRVYKN  + + P+P  QPMGVYSTLWEADDWATRG   EK N ++  F    K F+   G PVPGP +
Sbjct:   31 PAKFEDDFRIAWSDTHITQIDGGRAIQLKLDPSSGCGFASKKQYLFGRVSMKIKLIPGDSAGTVTAFYMNSDTDSVRDELDFEFLGNRSGQPYTVQTNVFAHGKGDREQRVNLWFDPSRDFHEYAISWNHLRIVFYVDNVPIRVYKNNEARKVPYPRFQPMGVYSTLWEADDWATRG-GIEKINWSRAPFYAYYKDFDI-EGCPVPGPAD 238          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH_TOBAC (Probable xyloglucan endotransglucosylase/hydrolase protein OS=Nicotiana tabacum GN=XTH PE=2 SV=1)

HSP 1 Score: 229.565 bits (584), Expect = 2.748e-60
Identity = 104/174 (59.77%), Postives = 131/174 (75.29%), Query Frame = 2
Query:   11 FLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSG--RAPFPMNQPMGVYSTLWEADDWATRG 526
            F  +++ +W+  H++ + GG  + LVLD++SG GF SK+ YLFG  SMK+KLV GDSAG VTAFY++SN     DE+DFEFLGNRTGQPY +QTN++  GKGDREQR+ LWFDP   YH Y++LWN   IV +VDDVPIR +KNS      FP NQPM +YS+LW+ADDWATRG
Sbjct:   32 FWKNYEPSWASHHIKYLSGGSTVDLVLDRSSGAGFQSKKSYLFGHFSMKLKLVGGDSAGVVTAFYLSSNNAE-HDEIDFEFLGNRTGQPYILQTNVFTGGKGDREQRIYLWFDPTKGYHSYSVLWNTFQIVIFVDDVPIRAFKNSKDLGVKFPFNQPMKIYSSLWDADDWATRG 204          

HSP 2 Score: 23.483 bits (49), Expect = 2.748e-60
Identity = 9/16 (56.25%), Postives = 11/16 (68.75%), Query Frame = 1
Query:  538 KIEWSKAPFYA*YKNF 585
            K +WS APF A Y +F
Sbjct:  208 KTDWSNAPFTASYTSF 223          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTHA_PHAAN (Xyloglucan endotransglucosylase/hydrolase protein A OS=Phaseolus angularis GN=XTHA PE=1 SV=1)

HSP 1 Score: 231.491 bits (589), Expect = 6.070e-60
Identity = 113/187 (60.43%), Postives = 137/187 (73.26%), Query Frame = 2
Query:   32 TWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSG--RAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTF 586
            TW+  H++ + GG  IQL LD+ +G GF SK  YLFG  SM IKLVPGDSAGTVTAFY++S T    DE+DFEFLGNRTGQPY +QTN++  GKGDREQR+ LWFDP   YH Y++LWN + IVFYVDD PIRV+KNS      FP NQPM +Y++LW ADDWATRG   EK + +K  F+ + K F
Sbjct:   38 TWAFDHIKYLNGGSEIQLHLDKYTGTGFQSKGSYLFGHFSMYIKLVPGDSAGTVTAFYLSS-TNAEHDEIDFEFLGNRTGQPYILQTNVFTGGKGDREQRIYLWFDPTTQYHRYSVLWNMYQIVFYVDDYPIRVFKNSNDLGVKFPFNQPMKIYNSLWNADDWATRG-GLEKTDWSKAPFIASYKGF 222          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH5_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 5 OS=Arabidopsis thaliana GN=XTH5 PE=2 SV=1)

HSP 1 Score: 229.95 bits (585), Expect = 1.766e-59
Identity = 110/187 (58.82%), Postives = 137/187 (73.26%), Query Frame = 2
Query:   32 TWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSG--RAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTF 586
            TW+  H++ + GG  + LVLD+ +G GF SK  YLFG  SM IK+V GDSAGTVTAFY++S      DE+DFEFLGNRTGQPY +QTN++  G G+REQR+NLWFDP+ DYH Y++LWN + IVF+VDDVPIRV+KNS      FP NQPM +YS+LW ADDWATRG   EK N  K  F+ + + F
Sbjct:   39 TWAFDHIKYLNGGSEVHLVLDKYTGTGFQSKGSYLFGHFSMHIKMVAGDSAGTVTAFYLSSQNSE-HDEIDFEFLGNRTGQPYILQTNVFTGGAGNREQRINLWFDPSKDYHSYSVLWNMYQIVFFVDDVPIRVFKNSKDVGVKFPFNQPMKIYSSLWNADDWATRG-GLEKTNWEKAPFVASYRGF 223          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH25_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 25 OS=Arabidopsis thaliana GN=XTH25 PE=2 SV=2)

HSP 1 Score: 217.624 bits (553), Expect = 3.258e-58
Identity = 100/184 (54.35%), Postives = 131/184 (71.20%), Query Frame = 2
Query:    8 TFLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSGR--APFPMNQPMGVYSTLWEADDWATRG------WSK 535
            TF  +F +TW D   + +  G  + L LD+ SG GF +K++YLFG++ M++KLVPG+SAGTVTA+Y+ S  +   DE+DFEFLGN TG PYT+ TN+Y  GKGDREQ+ +LWFDP AD+H Y++LWN HHIVF VDD+P+R +KN       +P  QPM +YS+LW AD WATRG      WSK
Sbjct:   29 TFDTEFDITWGDGRGKVLNNGELLTLSLDRASGSGFQTKKEYLFGKIDMQLKLVPGNSAGTVTAYYLKSKGDTW-DEIDFEFLGNLTGDPYTMHTNVYTQGKGDREQQFHLWFDPTADFHTYSVLWNPHHIVFMVDDIPVREFKNLQHMGIQYPKLQPMRLYSSLWNADQWATRGGLVKTDWSK 211          

HSP 2 Score: 28.4906 bits (62), Expect = 3.258e-58
Identity = 11/16 (68.75%), Postives = 13/16 (81.25%), Query Frame = 1
Query:  538 KIEWSKAPFYA*YKNF 585
            K +WSKAPF A Y+NF
Sbjct:  206 KTDWSKAPFTASYRNF 221          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH1_SOLLC (Probable xyloglucan endotransglucosylase/hydrolase 1 OS=Solanum lycopersicum GN=XTH1 PE=2 SV=1)

HSP 1 Score: 225.328 bits (573), Expect = 4.350e-58
Identity = 107/194 (55.15%), Postives = 139/194 (71.65%), Query Frame = 2
Query:   11 FLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSG--RAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTF 586
            F  +++ +W+  H++ + GG    L+LD++SG GF SK+ YLFG  SMK++LV GDSAG VTAFY++SN     DE+DFEFLGNRTGQPY +QTN++  GKG+REQR+ LWFDP   YH Y++LWN + IV +VDDVPIR +KNS      FP NQPM +YS+LW+ADDWATRG   EK N A   F  +  +F
Sbjct:   33 FWKNYEPSWASHHIKFLNGGTTTDLILDRSSGAGFQSKKSYLFGHFSMKMRLVGGDSAGVVTAFYLSSNNAE-HDEIDFEFLGNRTGQPYILQTNVFTGGKGNREQRIYLWFDPTKGYHSYSVLWNTYLIVIFVDDVPIRAFKNSKDLGVKFPFNQPMKIYSSLWDADDWATRG-GLEKTNWANAPFTASYTSF 224          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH_WHEAT (Probable xyloglucan endotransglucosylase/hydrolase OS=Triticum aestivum GN=XTH PE=2 SV=1)

HSP 1 Score: 224.942 bits (572), Expect = 5.682e-58
Identity = 107/194 (55.15%), Postives = 140/194 (72.16%), Query Frame = 2
Query:   11 FLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSG--RAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTF 586
            F  ++  TW+  H+  + GGR +QL LD+ +G GF ++  YLFG  SM IKLV GDSAGTVTAFY++S      DE+DFEFLGNRTGQPY +QTN+++ GKGDREQR+ LWFDP  DYH Y++LWN + I F+VDD PIRV+KNS      +P +QPM +YS+LW ADDWATRG  +EK + +K  F+ + + F
Sbjct:   32 FDKNYVPTWAQDHIHYVNGGREVQLSLDKTTGTGFQTRGSYLFGHFSMHIKLVGGDSAGTVTAFYLSSQNSE-HDEIDFEFLGNRTGQPYILQTNVFSGGKGDREQRIYLWFDPTKDYHSYSVLWNLYMIAFFVDDTPIRVFKNSKDLGVRYPFDQPMKLYSSLWNADDWATRG-GREKTDWSKAPFVASYRGF 223          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH_SOYBN (Probable xyloglucan endotransglucosylase/hydrolase (Fragment) OS=Glycine max GN=XTH PE=2 SV=1)

HSP 1 Score: 224.172 bits (570), Expect = 9.691e-58
Identity = 110/187 (58.82%), Postives = 136/187 (72.73%), Query Frame = 2
Query:   32 TWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSG--RAPFPMNQPMGVYSTLWEADDWATRGWSKEK*NGAKLLFMLNTKTF 586
            TW+  H++   GG  IQL LD+ +G GF SK  YLFG  SM IK+VPGDSAGTVTAFY++S      DE+DFEFLGNRTGQPY +QTN++  GKGDREQR+ LWFDP  +YH Y+ILWN + IVF+VD+VPIRV+KNS      FP +QPM +Y++LW ADDWATRG   EK + +K  F+   K F
Sbjct:   40 TWAFDHIKYFNGGSDIQLHLDKYTGTGFQSKGSYLFGHFSMYIKMVPGDSAGTVTAFYLSSQNAE-HDEIDFEFLGNRTGQPYILQTNVFTGGKGDREQRIYLWFDPTKEYHRYSILWNLYQIVFFVDEVPIRVFKNSKDLGVKFPFDQPMKIYNSLWNADDWATRG-GLEKTDWSKAPFIAAYKGF 224          
BLAST of EY675446 vs. ExPASy Swiss-Prot
Match: XTH15_ARATH (Probable xyloglucan endotransglucosylase/hydrolase protein 15 OS=Arabidopsis thaliana GN=XTH15 PE=1 SV=1)

HSP 1 Score: 218.009 bits (554), Expect = 1.227e-57
Identity = 100/185 (54.05%), Postives = 133/185 (71.89%), Query Frame = 2
Query:    5 ATFLDDFKVTWSDAHLRQIEGGRAIQLVLDQNSGCGFASKRQYLFGRVSMKIKLVPGDSAGTVTAFYMNSNTENVRDELDFEFLGNRTGQPYTVQTNIYANGKGDREQRVNLWFDPAADYHLYTILWNHHHIVFYVDDVPIRVYKNSGR--APFPMNQPMGVYSTLWEADDWATRG------WSK 535
            + F D+F +TW D   +   GG  + L LDQ SG GF SK++YLFGR+ M++KLV G+SAGTVTA+Y++S      DE+DFEFLGN TG+PY + TN++A GKGDREQ+  LWFDP  ++H Y+I+W   HI+F VD++PIRV+ N+ +   PFP +QPM +YS+LW ADDWATRG      WSK
Sbjct:   26 SNFFDEFDLTWGDHRGKIFNGGNMLSLSLDQVSGSGFKSKKEYLFGRIDMQLKLVAGNSAGTVTAYYLSSQGAT-HDEIDFEFLGNETGKPYVLHTNVFAQGKGDREQQFYLWFDPTKNFHTYSIVWRPQHIIFLVDNLPIRVFNNAEKLGVPFPKSQPMRIYSSLWNADDWATRGGLVKTDWSK 209          

HSP 2 Score: 26.1794 bits (56), Expect = 1.227e-57
Identity = 10/16 (62.50%), Postives = 12/16 (75.00%), Query Frame = 1
Query:  538 KIEWSKAPFYA*YKNF 585
            K +WSKAPF A Y+ F
Sbjct:  204 KTDWSKAPFTAYYRGF 219          
The following BLAST results are available for this feature:
BLAST of EY675446 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 44
Match NameE-valueIdentityDescription
XTH6_ARATH8.412e-8673.21Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH7_ARATH7.873e-8471.43Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH_TOBAC2.748e-6059.77Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTHA_PHAAN6.070e-6060.43Xyloglucan endotransglucosylase/hydrolase protein ... [more]
XTH5_ARATH1.766e-5958.82Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH25_ARATH3.258e-5854.35Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH1_SOLLC4.350e-5855.15Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH_WHEAT5.682e-5855.15Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH_SOYBN9.691e-5858.82Probable xyloglucan endotransglucosylase/hydrolase... [more]
XTH15_ARATH1.227e-5754.05Probable xyloglucan endotransglucosylase/hydrolase... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-401-010-F05-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY675446 ID=EY675446; Name=EY675446; organism=Citrus sinensis; type=EST; length=1015bp
ACCGGCTACTTTTCTTGACGATTTTAAAGTCACATGGTCCGATGCCCATC
TCAGGCAAATCGAGGGAGGGAGGGCCATCCAGCTAGTTCTAGACCAAAAT
TCAGGATGTGGGTTTGCTTCCAAAAGGCAGTACTTGTTCGGGCGTGTAAG
CATGAAGATCAAGCTCGTTCCTGGAGACTCTGCTGGAACTGTCACTGCAT
TTTATATGAATTCCAACACCGAAAATGTGAGAGACGAGCTTGATTTTGAG
TTCCTGGGAAACCGAACGGGTCAGCCTTACACGGTCCAGACCAATATCTA
TGCAAATGGAAAAGGCGATAGAGAACAGAGAGTGAACCTCTGGTTTGACC
CTGCTGCAGATTATCACCTTTACACTATTCTCTGGAACCATCACCATATT
GTCTTCTATGTGGATGATGTTCCCATCAGGGTTTACAAAAACAGTGGAAG
GGCTCCATTTCCAATGAACCAACCCATGGGGGTGTATTCGACGCTGTGGG
AAGCAGATGACTGGGCAACAAGAGGGTGGTCTAAAGAAAAATAGAATGGA
GCAAAGCTCCTTTTTATGCTTAATACAAAAACTTTTGAATATTGAAGGGG
CGGCCCAGTCCCGGGACCAACAAATGGTGGCCTCAAACCCAGGTAAAACG
GGGGGGGGAAGGAAAATAAATTATAAAACCCCCCACCCCCCAAGAAAAAA
AAAAAAAAAAACAAAAAAGGGTTTCGTCCCCCACCCCCCCATGAATCCTA
GATTTTATTGGCCCTGGAAAAAATCAAGAAAACCCCAAAACCCCCCCCGG
AAAAGCCCTTGGCCGGGAATAAAAATTTAAACCCCCCCCCAATTTAAATA
AAACCAGTTGGGGAGAGGGGACCCCCCCAAATCCCCCTAATTAAAACCCA
GGGTTGAAATATTACCTCTCCCCGGGTGACCACGCCCCCCTTTTTTGGTT
TTTTTTATTCCCCCCCCTTTTCTCCGTGGGGGAGAAAAAAAAAACCAAAT
ATTATTATAAACCAC
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