EY674965 | Citrus Genome Database

Overview


Name	EY674965
Unique Name	EY674965
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	992

Libraries


Library Name	Type
Sweet orange leaf, infected with CiLV	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB21_SINAL (Chlorophyll a-b binding protein 1, chloroplastic OS=Sinapis alba GN=CAB1 PE=3 SV=1)

HSP 1 Score: 172.555 bits (436), Expect = 3.254e-42
Identity = 87/150 (58.00%), Postives = 96/150 (64.00%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEPKPDPRSKXXXXXXXXXXXXXXXXKVQNCWRAAPEKRKNQCTPKDNFDPMG*AEDREVY 451
            LGPFSGEPPSYLTGEFPGDYGWDTAGLSADP+TFA+NRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P                         +        + ++   P  +FDP+G A D E +
Sbjct:   58 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPETFARNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNPSLVHAQSILAIWATQVILMGAVEGYRVAGEGPLGEAEDLLYPGGSFDPLGLATDPEAF 207

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB2B_PINSY (Chlorophyll a-b binding protein type 2 member 1B, chloroplastic OS=Pinus sylvestris PE=2 SV=1)

HSP 1 Score: 171.014 bits (432), Expect = 5.225e-42
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGEPPSYLTGEFPGDYGWDTAGLSADP+ FAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   67 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPENFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGAQIFSEGGLDYLGNP 158

HSP 2 Score: 22.3274 bits (46), Expect = 5.225e-42
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  178 GAVEGYRIAGGPLGEVTDPIYPGGSFDPLGLAEDPEA 214

HSP 3 Score: 84.3445 bits (207), Expect = 1.165e-15
Identity = 40/50 (80.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + GA IFSEG LDYLGNPSL+HAQSILAIWACQV+LMG    YRIAGGPL
Sbjct:  141 KAGAQIFSEGGLDYLGNPSLVHAQSILAIWACQVILMGAVEGYRIAGGPL 190

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB22_MAIZE (Chlorophyll a-b binding protein, chloroplastic OS=Zea mays PE=2 SV=1)

HSP 1 Score: 170.629 bits (431), Expect = 1.157e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGP SGEPPSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   58 LGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 149

HSP 2 Score: 21.557 bits (44), Expect = 1.157e-41
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  169 GAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLADDPEA 205

HSP 3 Score: 83.9593 bits (206), Expect = 1.522e-15
Identity = 41/50 (82.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSLIHAQSILAIWACQVVLMG    YRIAGGPL
Sbjct:  132 KAGSQIFSEGGLDYLGNPSLIHAQSILAIWACQVVLMGAVEGYRIAGGPL 181

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB2B_SOLLC (Chlorophyll a-b binding protein 1B, chloroplastic OS=Solanum lycopersicum GN=CAB1B PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.157e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGE PSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   58 LGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 149

HSP 2 Score: 22.3274 bits (46), Expect = 1.157e-41
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  169 GAVEGYRIAGGPLGEVVDPLYPGGSFDPLGLAEDPEA 205

HSP 3 Score: 83.5741 bits (205), Expect = 1.988e-15
Identity = 40/50 (80.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSL+HAQSILAIWACQVVLMG    YRIAGGPL
Sbjct:  132 KAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGAVEGYRIAGGPL 181

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB21_WHEAT (Chlorophyll a-b binding protein, chloroplastic OS=Triticum aestivum GN=WHAB1.6 PE=3 SV=1)

HSP 1 Score: 167.162 bits (422), Expect = 1.157e-41
Identity = 78/92 (84.78%), Postives = 79/92 (85.87%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGP SGEPPSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEA WFKAG      G    LG P
Sbjct:   59 LGPLSGEPPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAGWFKAGSQIFSDGGLDYLGNP 150

HSP 2 Score: 25.0238 bits (53), Expect = 1.157e-41
Identity = 12/37 (32.43%), Postives = 18/37 (48.65%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL  RP++
Sbjct:  170 GAVEGYRIAGGPLGEIVDPLYPGGSFDPLGLAERPQA 206

HSP 3 Score: 81.6481 bits (200), Expect = 7.553e-15
Identity = 38/50 (76.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFS+G LDYLGNPSL+HAQS+LAIWACQVVLMG    YRIAGGPL
Sbjct:  133 KAGSQIFSDGGLDYLGNPSLVHAQSLLAIWACQVVLMGAVEGYRIAGGPL 182

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB27_TOBAC (Chlorophyll a-b binding protein 7, chloroplastic OS=Nicotiana tabacum GN=CAB7 PE=3 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.507e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGE PSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   60 LGPFSGEAPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 151

HSP 2 Score: 21.9422 bits (45), Expect = 1.507e-41
Identity = 12/37 (32.43%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G IEG  +  G  GE  +P+Y        GL   P +
Sbjct:  171 GAIEGYRVAGGPLGEVTDPLYPGGSFDPLGLADDPEA 207

HSP 3 Score: 83.1889 bits (204), Expect = 2.596e-15
Identity = 39/50 (78.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSL+HAQSILAIWACQVVLMG    YR+AGGPL
Sbjct:  134 KAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGAIEGYRVAGGPL 183

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB25_TOBAC (Chlorophyll a-b binding protein 50, chloroplastic OS=Nicotiana tabacum GN=CAB50 PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.507e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGE PSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   60 LGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 151

HSP 2 Score: 21.9422 bits (45), Expect = 1.507e-41
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  171 GAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEA 207

HSP 3 Score: 83.1889 bits (204), Expect = 2.596e-15
Identity = 39/50 (78.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSL+HAQSILAIWACQVVLMG    YR+AGGPL
Sbjct:  134 KAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGAVEGYRVAGGPL 183

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB24_TOBAC (Chlorophyll a-b binding protein 40, chloroplastic OS=Nicotiana tabacum GN=CAB40 PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.507e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGE PSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   60 LGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 151

HSP 2 Score: 21.9422 bits (45), Expect = 1.507e-41
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  171 GAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEA 207

HSP 3 Score: 83.1889 bits (204), Expect = 2.596e-15
Identity = 39/50 (78.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSL+HAQSILAIWACQVVLMG    YR+AGGPL
Sbjct:  134 KAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGAVEGYRVAGGPL 183

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB22_TOBAC (Chlorophyll a-b binding protein 21, chloroplastic OS=Nicotiana tabacum GN=CAB21 PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.507e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGE PSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   58 LGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 149

HSP 2 Score: 21.9422 bits (45), Expect = 1.507e-41
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  169 GAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEA 205

HSP 3 Score: 82.8037 bits (203), Expect = 3.391e-15
Identity = 38/50 (76.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSL+HAQSILAIWACQV+LMG    YR+AGGPL
Sbjct:  132 KAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVILMGAVEGYRVAGGPL 181

[Close]

BLAST of EY674965 vs. ExPASy Swiss-Prot
Match: CB21_TOBAC (Chlorophyll a-b binding protein 16, chloroplastic OS=Nicotiana tabacum GN=CAB16 PE=2 SV=1)

HSP 1 Score: 169.859 bits (429), Expect = 1.507e-41
Identity = 79/92 (85.87%), Postives = 80/92 (86.96%), Query Frame = 2

Query:    2 LGPFSGEPPSYLTGEFPGDYGWDTAGLSADPKTFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGGPYLQRGQA*LLGEP 277
            LGPFSGE PSYLTGEFPGDYGWDTAGLSADP+TFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAG      G    LG P
Sbjct:   59 LGPFSGESPSYLTGEFPGDYGWDTAGLSADPETFAKNRELEVIHCRWAMLGALGCVFPELLARNGVKFGEAVWFKAGSQIFSEGGLDYLGNP 150

HSP 2 Score: 21.9422 bits (45), Expect = 1.507e-41
Identity = 11/37 (29.73%), Postives = 16/37 (43.24%), Query Frame = 1

Query:  337 GPIEGTELLEGRSGEEKEPMYAKRQL*SNGLGRRPRS 447
            G +EG  +  G  GE  +P+Y        GL   P +
Sbjct:  170 GAVEGYRVAGGPLGEVVDPLYPGGSFDPLGLAEDPEA 206

HSP 3 Score: 83.1889 bits (204), Expect = 2.596e-15
Identity = 39/50 (78.00%), Postives = 43/50 (86.00%), Query Frame = 3

Query:  225 RRGAHIFSEGRLDYLGNPSLIHAQSILAIWACQVVLMGTH*RYRIAGGPL 374
            + G+ IFSEG LDYLGNPSL+HAQSILAIWACQVVLMG    YR+AGGPL
Sbjct:  133 KAGSQIFSEGGLDYLGNPSLVHAQSILAIWACQVVLMGAVEGYRVAGGPL 182

The following BLAST results are available for this feature:

BLAST of EY674965 vs. ExPASy Swiss-Prot

BLAST of EY674965 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 65


Match Name	E-value	Identity	Description
CB21_SINAL	3.254e-42	58.00	Chlorophyll a-b binding protein 1, chloroplastic O...	[more]
CB2B_PINSY	5.225e-42	85.87	Chlorophyll a-b binding protein type 2 member 1B, ...	[more]
CB22_MAIZE	1.157e-41	85.87	Chlorophyll a-b binding protein, chloroplastic OS=...	[more]
CB2B_SOLLC	1.157e-41	85.87	Chlorophyll a-b binding protein 1B, chloroplastic ...	[more]
CB21_WHEAT	1.157e-41	84.78	Chlorophyll a-b binding protein, chloroplastic OS=...	[more]
CB27_TOBAC	1.507e-41	85.87	Chlorophyll a-b binding protein 7, chloroplastic O...	[more]
CB25_TOBAC	1.507e-41	85.87	Chlorophyll a-b binding protein 50, chloroplastic ...	[more]
CB24_TOBAC	1.507e-41	85.87	Chlorophyll a-b binding protein 40, chloroplastic ...	[more]
CB22_TOBAC	1.507e-41	85.87	Chlorophyll a-b binding protein 21, chloroplastic ...	[more]
CB21_TOBAC	1.507e-41	85.87	Chlorophyll a-b binding protein 16, chloroplastic ...	[more]

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Properties


Property Name	Value
Genbank description	CS00-C1-401-004-A01-CT.F Sweet orange leaf, infected with CiLV Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY674965 ID=EY674965; Name=EY674965; organism=Citrus sinensis; type=EST; length=992bp
CTTGGGTCCATTCTCTGGCGAGCCCCCAAGCTACTTGACTGGTGAGTTCC
CTGGTGACTACGGTTGGGACACTGCTGGGCTTTCAGCTGACCCAAAAACC
TTTGCCAAGAACCGTGAACTCGAAGTCATCCACTGCAGATGGGCCATGTT
GGGAGCTCTTGGATGCGTCTTCCCGGAGCTCTTGGCCCGCAACGGAGTCA
AGTTCGGTGAGGCCGTGTGGTTCAAGGCGGGGGGCCCATATCTTCAGCGA
GGGCAGGCTTGACTACTTGGGGAACCCAAGCCTGATCCACGCTCAAAGCA
TCTTGGCCATCTGGGCTTGCCAGGTGGTCTTGATGGGGACCCATTGAAGG
TACAGAATTGCTGGAGGGCCGCTCCGGAGAAGAGAAAGAACCAATGTACG
CCAAAAGACAACTTTGATCCAATGGGTTAGGCAGAAGACCGAGAAGTTTA
TGGTAGAGCCCAAGGATAAGAGAAACAAGAAAAGGATAGATGGGGAAGTG
AAGACGTGGTGTTGGGTATCAGTCCTTTAAAGGGAATTTAAAAAAGGAGA
GGGGAACAATAAAAAAAAACTTGGGATAAACACGTTGGGAAAGGATCGTA
AGGAGAAGAAGGCCTTGAGAACGAAAGAACCTAAAAAATGGCCCACGAGG
AAAAAGAGAAAACCAGGAGAGAATAAAAAGAAAAAGACAACTTTTGGGGG
ATGAAGACGAAACACAAGCAGCAAGAAGTAGAGAGGGGAAAGAGCGCAGG
TGGGGACAGGGTGAAGACTTGGATAGAGAGACTCCTACATAATTAAGTGG
AAAAAATGGACAAATATTAAGAAAGTAAATAGATCACAAAGATAGGATGA
ACAATCAGGTAATGATAAAATATGAGAAAACACCGGAAGTAGCTAGGAGT
GGAAGCATGCAAAAAAGAGGGCGATATATTGGCAGAATGAAGAATGTAGA
GAAATAATGTGAATAAAAAAGAGGAATTGTAGTGAGTGGAGA