EY703406 | Citrus Genome Database

Overview


Name	EY703406
Unique Name	EY703406
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	915

Libraries


Library Name	Type
Sweet orange fruit, development stadium (2 of 6)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UEV1D_ARATH (Ubiquitin-conjugating enzyme E2 variant 1D OS=Arabidopsis thaliana GN=UEV1D PE=1 SV=1)

HSP 1 Score: 238.039 bits (606), Expect = 3.414e-72
Identity = 107/120 (89.17%), Postives = 113/120 (94.17%), Query Frame = 3

Query:  210 KSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDGTYF 569
            K IGDGTVSYGMDDGDDIYMRSWTGT+IGPHNTVHEGRIYQLKLFCDKDYPEKPP+VRFHSR+NM CVNHETGVV+PKKFGLLANWQREYTMEDIL QL KEM+  HNRKLVQPP+GT F
Sbjct:   27 KGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLFCDKDYPEKPPTVRFHSRVNMACVNHETGVVDPKKFGLLANWQREYTMEDILVQLKKEMSTSHNRKLVQPPEGTCF 146

HSP 2 Score: 55.8398 bits (133), Expect = 3.414e-72
Identity = 27/27 (100.00%), Postives = 27/27 (100.00%), Query Frame = 1

Query:  133 MTLGSGGSSVVVPRNFRLLEELERGEK 213
            MTLGSGGSSVVVPRNFRLLEELERGEK
Sbjct:    1 MTLGSGGSSVVVPRNFRLLEELERGEK 27

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UEV1C_ARATH (Ubiquitin-conjugating enzyme E2 variant 1C OS=Arabidopsis thaliana GN=UEV1C PE=1 SV=1)

HSP 1 Score: 240.35 bits (612), Expect = 8.264e-71
Identity = 108/120 (90.00%), Postives = 116/120 (96.67%), Query Frame = 3

Query:  210 KSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDGTYF 569
            K IGDGTVSYGMDDGDDIYMRSWTGT+IGPHNTVHEGRIYQLKLFCDKDYPEKPP+VRFHSRINMTCVNH+TGVV+ KKFG+LANWQR+YTMEDILTQL KEMAA HNRKLVQPP+GT+F
Sbjct:   26 KGIGDGTVSYGMDDGDDIYMRSWTGTIIGPHNTVHEGRIYQLKLFCDKDYPEKPPTVRFHSRINMTCVNHDTGVVDSKKFGVLANWQRQYTMEDILTQLKKEMAASHNRKLVQPPEGTFF 145

HSP 2 Score: 48.9062 bits (115), Expect = 8.264e-71
Identity = 26/27 (96.30%), Postives = 26/27 (96.30%), Query Frame = 1

Query:  133 MTLGSGGSSVVVPRNFRLLEELERGEK 213
            MTLGSG SSVVVPRNFRLLEELERGEK
Sbjct:    1 MTLGSG-SSVVVPRNFRLLEELERGEK 26

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UEV1A_ARATH (Ubiquitin-conjugating enzyme E2 variant 1A OS=Arabidopsis thaliana GN=UEV1A PE=1 SV=1)

HSP 1 Score: 191.43 bits (485), Expect = 1.598e-53
Identity = 85/117 (72.65%), Postives = 98/117 (83.76%), Query Frame = 3

Query:  210 KSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDG 560
            K IGDGTVSYGMDD DDIYM+SWTGT++GP NT +EG+I+QLKLFC K+YPE PP+VRF +RINM CVN ETGVVEP  F +L NW+REYTMEDIL +L KEM   HNRKL QPP+G
Sbjct:   25 KGIGDGTVSYGMDDADDIYMQSWTGTILGPPNTAYEGKIFQLKLFCGKEYPESPPTVRFQTRINMACVNPETGVVEPSLFPMLTNWRREYTMEDILVKLKKEMMTSHNRKLAQPPEG 141

HSP 2 Score: 40.0466 bits (92), Expect = 1.598e-53
Identity = 19/25 (76.00%), Postives = 21/25 (84.00%), Query Frame = 1

Query:  139 LGSGGSSVVVPRNFRLLEELERGEK 213
            + S  + VVVPRNFRLLEELERGEK
Sbjct:    1 MSSEEAKVVVPRNFRLLEELERGEK 25

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UEV1B_ARATH (Ubiquitin-conjugating enzyme E2 variant 1B OS=Arabidopsis thaliana GN=UEV1B PE=1 SV=1)

HSP 1 Score: 184.882 bits (468), Expect = 3.848e-52
Identity = 82/117 (70.09%), Postives = 97/117 (82.91%), Query Frame = 3

Query:  210 KSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDG 560
            K IGDGTVSYGMDD DDI M+SWTGT++GPHNT +EG+I+QLKLFC KDYPE PP+VRF SRINM CVN E GVV+P  F +L+NW+RE+TMED+L QL KEM +  NRKL QP +G
Sbjct:   25 KGIGDGTVSYGMDDADDILMQSWTGTILGPHNTAYEGKIFQLKLFCGKDYPESPPTVRFQSRINMACVNPENGVVDPSHFPMLSNWRREFTMEDLLIQLKKEMMSSQNRKLAQPLEG 141

HSP 2 Score: 41.9726 bits (97), Expect = 3.848e-52
Identity = 20/25 (80.00%), Postives = 21/25 (84.00%), Query Frame = 1

Query:  139 LGSGGSSVVVPRNFRLLEELERGEK 213
            +GS    VVVPRNFRLLEELERGEK
Sbjct:    1 MGSEEEKVVVPRNFRLLEELERGEK 25

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UBE2V_DICDI (Probable ubiquitin-conjugating enzyme E2 variant OS=Dictyostelium discoideum GN=ube2v PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 3.859e-39
Identity = 65/120 (54.17%), Postives = 91/120 (75.83%), Query Frame = 3

Query:  210 KSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDGTYF 569
            KS GDGT+SYG++  DDI + SW GT+IGP NT  + R Y LKL+CDKDYP KPP+VRF ++IN+ CVN + GVV+PK   +L+NW  +  +++IL++L +EM++  N+KLVQPP+G  F
Sbjct:   19 KSTGDGTISYGLESPDDILLSSWIGTIIGPQNTPFDNRFYSLKLYCDKDYPNKPPTVRFTTKINIGCVNQQNGVVDPKNLPILSNWTSKNRIQNILSELRREMSSSSNKKLVQPPEGANF 138

HSP 2 Score: 30.0314 bits (66), Expect = 3.859e-39
Identity = 13/16 (81.25%), Postives = 14/16 (87.50%), Query Frame = 1

Query:  166 VPRNFRLLEELERGEK 213
            +PRNF LLEELE GEK
Sbjct:    4 IPRNFVLLEELESGEK 19

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: MMS2_SCHPO (Ubiquitin-conjugating enzyme spm2 OS=Schizosaccharomyces pombe GN=spm2 PE=1 SV=1)

HSP 1 Score: 135.961 bits (341), Expect = 4.040e-35
Identity = 61/120 (50.83%), Postives = 83/120 (69.17%), Query Frame = 3

Query:  210 KSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDGTYF 569
            K +G+ + SYG+ + DDI +  W  T++GP ++VHE RIY LK+ CD +YP+ PP V F SRIN+  V+ ETG V P K   L +W+REY+ME +L  L KEMA+  NRKL QPP+G+ F
Sbjct:   19 KGLGESSCSYGLTNADDITLSDWNATILGPAHSVHENRIYSLKIHCDANYPDAPPIVTFVSRINLPGVDGETGKVNPHKIDCLRHWKREYSMETVLLDLKKEMASSSNRKLPQPPEGSTF 138

HSP 2 Score: 33.8834 bits (76), Expect = 4.040e-35
Identity = 15/18 (83.33%), Postives = 17/18 (94.44%), Query Frame = 1

Query:  166 VPRNFRLLEELERGEKVL 219
            VPRNF+LLEELE+GEK L
Sbjct:    4 VPRNFKLLEELEKGEKGL 21

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UB2V1_HUMAN (Ubiquitin-conjugating enzyme E2 variant 1 OS=Homo sapiens GN=UBE2V1 PE=1 SV=2)

HSP 1 Score: 129.028 bits (323), Expect = 4.352e-34
Identity = 56/118 (47.46%), Postives = 81/118 (68.64%), Query Frame = 3

Query:  207 RKSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDG 560
            +K +GDGTVS+G++D +D+ +  WTG +IGP  T++E RIY LK+ C   YPE PP VRF ++INM  VN   GVV+P+   +LA WQ  Y+++ +L +L + M +  N KL QPP+G
Sbjct:   25 QKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEG 142

HSP 2 Score: 37.3502 bits (85), Expect = 4.352e-34
Identity = 17/21 (80.95%), Postives = 18/21 (85.71%), Query Frame = 1

Query:  151 GSSVVVPRNFRLLEELERGEK 213
            GS V VPRNFRLLEELE G+K
Sbjct:    6 GSGVKVPRNFRLLEELEEGQK 26

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UB2V1_BOVIN (Ubiquitin-conjugating enzyme E2 variant 1 OS=Bos taurus GN=UBE2V1 PE=2 SV=1)

HSP 1 Score: 129.028 bits (323), Expect = 4.352e-34
Identity = 56/118 (47.46%), Postives = 81/118 (68.64%), Query Frame = 3

Query:  207 RKSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDG 560
            +K +GDGTVS+G++D +D+ +  WTG +IGP  T++E RIY LK+ C   YPE PP VRF ++INM  VN   GVV+P+   +LA WQ  Y+++ +L +L + M +  N KL QPP+G
Sbjct:   25 QKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEG 142

HSP 2 Score: 37.3502 bits (85), Expect = 4.352e-34
Identity = 17/21 (80.95%), Postives = 18/21 (85.71%), Query Frame = 1

Query:  151 GSSVVVPRNFRLLEELERGEK 213
            GS V VPRNFRLLEELE G+K
Sbjct:    6 GSGVKVPRNFRLLEELEEGQK 26

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UB2V1_MOUSE (Ubiquitin-conjugating enzyme E2 variant 1 OS=Mus musculus GN=Ube2v1 PE=1 SV=1)

HSP 1 Score: 128.257 bits (321), Expect = 7.383e-34
Identity = 56/118 (47.46%), Postives = 83/118 (70.34%), Query Frame = 3

Query:  207 RKSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDG 560
            +K +GDGTVS+G++D +D+ +  WTG +IGP  T++E RIY LK+ C   YPE PPSVRF +R+NM+ V+   GVV+P+   +LA WQ  ++++ IL +L + M +  N KL QPP+G
Sbjct:   25 QKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKYPEAPPSVRFVTRVNMSGVSSSNGVVDPRATAVLAKWQNSHSIKVILQELRRLMMSKENMKLPQPPEG 142

HSP 2 Score: 37.3502 bits (85), Expect = 7.383e-34
Identity = 17/21 (80.95%), Postives = 18/21 (85.71%), Query Frame = 1

Query:  151 GSSVVVPRNFRLLEELERGEK 213
            GS V VPRNFRLLEELE G+K
Sbjct:    6 GSGVKVPRNFRLLEELEEGQK 26

[Close]

BLAST of EY703406 vs. ExPASy Swiss-Prot
Match: UB2V1_PONAB (Ubiquitin-conjugating enzyme E2 variant 1 OS=Pongo abelii GN=UBE2V1 PE=2 SV=1)

HSP 1 Score: 125.561 bits (314), Expect = 4.695e-33
Identity = 55/118 (46.61%), Postives = 80/118 (67.80%), Query Frame = 3

Query:  207 RKSIGDGTVSYGMDDGDDIYMRSWTGTVIGPHNTVHEGRIYQLKLFCDKDYPEKPPSVRFHSRINMTCVNHETGVVEPKKFGLLANWQREYTMEDILTQLTKEMAAPHNRKLVQPPDG 560
            +K +GDGTVS+G++D +D+ +  WTG +IGP  T++E RIY LK+ C    PE PP VRF ++INM  VN   GVV+P+   +LA WQ  Y+++ +L +L + M +  N KL QPP+G
Sbjct:   25 QKGVGDGTVSWGLEDDEDMTLTRWTGMIIGPPRTIYENRIYSLKIECGPKCPEAPPFVRFVTKINMNGVNSSNGVVDPRAISVLAKWQNSYSIKVVLQELRRLMMSKENMKLPQPPEG 142

HSP 2 Score: 37.3502 bits (85), Expect = 4.695e-33
Identity = 17/21 (80.95%), Postives = 18/21 (85.71%), Query Frame = 1

Query:  151 GSSVVVPRNFRLLEELERGEK 213
            GS V VPRNFRLLEELE G+K
Sbjct:    6 GSGVKVPRNFRLLEELEEGQK 26

The following BLAST results are available for this feature:

BLAST of EY703406 vs. ExPASy Swiss-Prot

BLAST of EY703406 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 20


Match Name	E-value	Identity	Description
UEV1D_ARATH	3.414e-72	89.17	Ubiquitin-conjugating enzyme E2 variant 1D OS=Arab...	[more]
UEV1C_ARATH	8.264e-71	90.00	Ubiquitin-conjugating enzyme E2 variant 1C OS=Arab...	[more]
UEV1A_ARATH	1.598e-53	72.65	Ubiquitin-conjugating enzyme E2 variant 1A OS=Arab...	[more]
UEV1B_ARATH	3.848e-52	70.09	Ubiquitin-conjugating enzyme E2 variant 1B OS=Arab...	[more]
UBE2V_DICDI	3.859e-39	54.17	Probable ubiquitin-conjugating enzyme E2 variant O...	[more]
MMS2_SCHPO	4.040e-35	50.83	Ubiquitin-conjugating enzyme spm2 OS=Schizosacchar...	[more]
UB2V1_HUMAN	4.352e-34	47.46	Ubiquitin-conjugating enzyme E2 variant 1 OS=Homo ...	[more]
UB2V1_BOVIN	4.352e-34	47.46	Ubiquitin-conjugating enzyme E2 variant 1 OS=Bos t...	[more]
UB2V1_MOUSE	7.383e-34	47.46	Ubiquitin-conjugating enzyme E2 variant 1 OS=Mus m...	[more]
UB2V1_PONAB	4.695e-33	46.61	Ubiquitin-conjugating enzyme E2 variant 1 OS=Pongo...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C3-701-019-B11-CT.F Sweet orange fruit, development stadium (2 of 6) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY703406 ID=EY703406; Name=EY703406; organism=Citrus sinensis; type=EST; length=915bp
CCACGCGTCCGCTTTAGGCTCGTCTTTGCTTGCCTTCCTCTTTTCCTGCA
AAGAACTCTTCTCTTCCTCCGACCCCTCTCCCCCTTCCGTGCTCTCTTAG
ATCTCAGCTTACCCATCATCAAAATCGCAACCATGACGCTAGGCTCAGGA
GGATCCAGTGTCGTGGTCCCTCGGAACTTCAGATTGTTGGAGGAACTTGA
ACGCGGAGAAAAAGTATTGGAGATGGCACTGTCAGCTATGGAATGGATGA
TGGAGATGACATTTACATGCGCTCTTGGACTGGCACCGTTATCGGTCCTC
ACAATACTGTACATGAAGGTAGAATTTATCAGTTGAAGCTGTTCTGTGAT
AAAGATTATCCAGAGAAGCCACCAAGTGTTCGTTTTCATTCACGAATCAA
CATGACTTGTGTTAACCATGAAACTGGAGTGGTGGAACCTAAAAAGTTTG
GACTTCTTGCGAATTGGCAGCGAGAGTACACCATGGAGGATATACTCACA
CAACTGACAAAGGAGATGGCAGCCCCACACAATCGGAAGCTGGTTCAACC
TCCTGATGGTACCTACTTCTAGCTTCTAAGATCATATAGGTATCATGGGG
GCTATTGCATATCACAATATGCAATATATAGATTGTATTCCTGTGTTGAT
GTTAAGAACATANAAGGAAATGCCTGAAAAGGCCTTTTNCATTACATCTN
CATGGCTTCATCATNCGTTTGGCTCTTTCTTTGGCTTTTTCCAACTGGCA
ATGGAGGATGCTGTTTGATTAAGGGGATTTCTGTGTGTCAATATTAAAAA
AAAAAAAAAAAAAAAGGCCGGCCGCTTTTAAGATCAAACTACCTACGCTG
CATGCGAGTTTAGTTTTTTATGGGAACCCAATTCATTCACGGCCCGCTTA
ACACGCGGAAAGGAA