EY650013 | Citrus Genome Database

Overview


Name	EY650013
Unique Name	EY650013
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	936

Libraries


Library Name	Type
Sweet orange leaf, greenhouse plant	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY650013_ssr80	microsatellite	EY650013_ssr80:80..89.	BLAST: Citrus ESTs to Prunus persica proteins V1
EY650013_ssr104	microsatellite	EY650013_ssr104:104..113.	BLAST: Citrus ESTs to Prunus persica proteins V1
EY650013_ssr208	microsatellite	EY650013_ssr208:208..221.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: VILI3_ARATH (Villin-3 OS=Arabidopsis thaliana GN=VLN3 PE=1 SV=1)

HSP 1 Score: 125.946 bits (315), Expect = 2.424e-43
Identity = 56/72 (77.78%), Postives = 63/72 (87.50%), Query Frame = 1

Query:  337 MATSAKSLDPAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            M+ S K LDPAFQG GQ+ GTEIWRIENF+PVP+PKSEHGKFYMGD YIVL+TT  KGGAYL DIHFW+GK+
Sbjct:    1 MSGSTKVLDPAFQGVGQKPGTEIWRIENFEPVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKD 72

HSP 2 Score: 69.3218 bits (168), Expect = 2.424e-43
Identity = 34/49 (69.39%), Postives = 38/49 (77.55%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE GTAA+KTVELDA LGGRAVQ+RE+QG  SDKFL Y K  +I   GG
Sbjct:   76 DEAGTAAVKTVELDAALGGRAVQYREIQGHESDKFLSYFKPCIIPLEGG 124

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: VILI2_ARATH (Villin-2 OS=Arabidopsis thaliana GN=VLN2 PE=1 SV=2)

HSP 1 Score: 122.865 bits (307), Expect = 5.362e-43
Identity = 55/69 (79.71%), Postives = 61/69 (88.41%), Query Frame = 1

Query:  346 SAKSLDPAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            S K LDPAFQGAGQ+ GTEIWRIENF+ VP+PKSEHGKFYMGD YIVL+TT  KGGAYL DIHFW+GK+
Sbjct:    2 STKVLDPAFQGAGQKPGTEIWRIENFEAVPVPKSEHGKFYMGDTYIVLQTTQNKGGAYLFDIHFWIGKD 70

HSP 2 Score: 71.2478 bits (173), Expect = 5.362e-43
Identity = 35/49 (71.43%), Postives = 38/49 (77.55%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE GTAA+KTVELDA LGGRAVQHRE+QG  SDKFL Y K  +I   GG
Sbjct:   74 DEAGTAAVKTVELDAVLGGRAVQHREIQGHESDKFLSYFKPCIIPLEGG 122

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: VILI4_ARATH (Villin-4 OS=Arabidopsis thaliana GN=VLN4 PE=1 SV=1)

HSP 1 Score: 102.449 bits (254), Expect = 2.049e-35
Identity = 46/72 (63.89%), Postives = 55/72 (76.39%), Query Frame = 1

Query:  337 MATSAKSLDPAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            M+ S + LDPAFQGAGQ+ G EIWRIENF P P+PKS  GKF+ GD YIVL+TT  K GA   DIH+W+GK+
Sbjct:    1 MSVSMRDLDPAFQGAGQKAGIEIWRIENFIPTPIPKSSIGKFFTGDSYIVLKTTALKTGALRHDIHYWLGKD 72

HSP 2 Score: 66.2402 bits (160), Expect = 2.049e-35
Identity = 32/49 (65.31%), Postives = 38/49 (77.55%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE GTAA+KTVELDA LGGRAVQ+RE+QG  ++KFL Y K  +I   GG
Sbjct:   76 DEAGTAAVKTVELDAALGGRAVQYREVQGHETEKFLSYFKPCIIPQEGG 124

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS_HOMAM (Gelsolin, cytoplasmic OS=Homarus americanus PE=1 SV=1)

HSP 1 Score: 74.7146 bits (182), Expect = 2.876e-23
Identity = 35/63 (55.56%), Postives = 42/63 (66.67%), Query Frame = 1

Query:  364 PAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            PAF+GAG   G  IWRIENF+ VP PK ++G+FY GD YIVL T    G     D+HFW+G E
Sbjct:    3 PAFEGAGAVEGLTIWRIENFEVVPYPKEKYGQFYQGDSYIVLYTRDVNGNLSW-DLHFWLGSE 64

HSP 2 Score: 53.1434 bits (126), Expect = 2.876e-23
Identity = 28/49 (57.14%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE GTAAIKTVELD  LGG  VQHRE++G  +  FL   K+ V +  GG
Sbjct:   68 DEAGTAAIKTVELDDQLGGVPVQHREVEGHETSLFLSRFKKGVRYLKGG 116

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS_DROME (Gelsolin OS=Drosophila melanogaster GN=Gel PE=1 SV=2)

HSP 1 Score: 75.8702 bits (185), Expect = 5.195e-22
Identity = 35/66 (53.03%), Postives = 44/66 (66.67%), Query Frame = 1

Query:  358 LDPAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLD-DIHFWMGKE 552
            + P+F  AG+  G EIWRIENF+PV  PK+ +GKFY GD +IVL T   K    L  D+HFW+G E
Sbjct:   59 MHPSFANAGRTPGLEIWRIENFEPVIYPKTNYGKFYTGDSFIVLNTIENKKDKKLSWDVHFWLGLE 124

HSP 2 Score: 47.7506 bits (112), Expect = 5.195e-22
Identity = 25/49 (51.02%), Postives = 29/49 (59.18%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE G AAI TV+LD  L G  VQHRE+Q   S  FL Y K  + +  GG
Sbjct:  128 DEAGAAAILTVQLDDLLNGGPVQHREVQDHESQLFLSYFKNGIRYEQGG 176

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS_CHICK (Gelsolin OS=Gallus gallus GN=GSN PE=2 SV=1)

HSP 1 Score: 67.0106 bits (162), Expect = 1.936e-21
Identity = 29/61 (47.54%), Postives = 39/61 (63.93%), Query Frame = 1

Query:  370 FQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            F  AG+  G +IWRIE F  VP+PK+ +G F+ GD Y+VL T   + G    D+HFW+G E
Sbjct:   55 FSKAGKEPGLQIWRIEKFDLVPVPKNLYGDFFTGDSYLVLNTIRQRSGNLQYDLHFWLGDE 115

HSP 2 Score: 54.6842 bits (130), Expect = 1.936e-21
Identity = 27/49 (55.10%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE G AAI TV++D  L G+AVQHRE+QG  S  FLGY K  + +  GG
Sbjct:  119 DERGAAAIFTVQMDDYLQGKAVQHREVQGHESSTFLGYFKSGIKYKAGG 167

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS1_LUMTE (Gelsolin-like protein 1 OS=Lumbricus terrestris GN=AM PE=1 SV=1)

HSP 1 Score: 70.8626 bits (172), Expect = 4.320e-21
Identity = 31/66 (46.97%), Postives = 44/66 (66.67%), Query Frame = 1

Query:  361 DPAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLET--TPGKGGAYLDDIHFWMGKE 552
            +PA++GAGQ+ G +IWRI NF+    P+++HGKFY GD YI+L T     K      D+HFW+G +
Sbjct:   37 EPAWKGAGQKEGLKIWRIVNFKVTEWPQNQHGKFYNGDSYIILNTYKPDPKSNELAYDVHFWIGSQ 102

HSP 2 Score: 49.6766 bits (117), Expect = 4.320e-21
Identity = 26/49 (53.06%), Postives = 31/49 (63.27%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE GTAA KTVELD  L  + VQHRE+QG  S+ F  Y K+ +    GG
Sbjct:  106 DEYGTAAYKTVELDTFLDDKPVQHREVQGYESELFRNYFKQGLTILEGG 154

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS_HORSE (Gelsolin OS=Equus caballus GN=GSN PE=1 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 5.550e-21
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1

Query:  364 PAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            P F  AG+  G +IWR+E F  VP+P + +G F+ GD Y++L+T   + G    D+H+W+G E
Sbjct:    6 PEFLKAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGILQYDLHYWLGNE 68

HSP 2 Score: 53.9138 bits (128), Expect = 5.550e-21
Identity = 29/49 (59.18%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE G AAI TV+LD  L GRAVQHRE+QG  S  FLGY K  + +  GG
Sbjct:   72 DESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGG 120

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS_HUMAN (Gelsolin OS=Homo sapiens GN=GSN PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.211e-21
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1

Query:  364 PAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            P F  AG+  G +IWR+E F  VP+P + +G F+ GD Y++L+T   + G    D+H+W+G E
Sbjct:   57 PEFLKAGKEPGLQIWRVEKFDLVPVPTNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNE 119

HSP 2 Score: 53.9138 bits (128), Expect = 7.211e-21
Identity = 29/49 (59.18%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE G AAI TV+LD  L GRAVQHRE+QG  S  FLGY K  + +  GG
Sbjct:  123 DESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGG 171

[Close]

BLAST of EY650013 vs. ExPASy Swiss-Prot
Match: GELS_PIG (Gelsolin (Fragment) OS=Sus scrofa GN=GSN PE=1 SV=1)

HSP 1 Score: 65.855 bits (159), Expect = 7.212e-21
Identity = 26/63 (41.27%), Postives = 40/63 (63.49%), Query Frame = 1

Query:  364 PAFQGAGQRVGTEIWRIENFQPVPLPKSEHGKFYMGDCYIVLETTPGKGGAYLDDIHFWMGKE 552
            P F  AG+  G +IWR+E F  VP+P + +G F+ GD Y++L+T   + G    D+H+W+G E
Sbjct:   47 PEFLKAGKEPGLQIWRVEKFDLVPVPPNLYGDFFTGDAYVILKTVQLRNGNLQYDLHYWLGNE 109

HSP 2 Score: 53.9138 bits (128), Expect = 7.212e-21
Identity = 29/49 (59.18%), Postives = 33/49 (67.35%), Query Frame = 2

Query:  560 DEXGTAAIKTVELDACLGGRAVQHRELQGRGSDKFLGYLKRWVIHWGGG 706
            DE G AAI TV+LD  L GRAVQHRE+QG  S  FLGY K  + +  GG
Sbjct:  113 DESGAAAIFTVQLDDYLNGRAVQHREVQGFESATFLGYFKSGLKYKKGG 161

The following BLAST results are available for this feature:

BLAST of EY650013 vs. ExPASy Swiss-Prot

BLAST of EY650013 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 29


Match Name	E-value	Identity	Description
VILI3_ARATH	2.424e-43	77.78	Villin-3 OS=Arabidopsis thaliana GN=VLN3 PE=1 SV=1	[more]
VILI2_ARATH	5.362e-43	79.71	Villin-2 OS=Arabidopsis thaliana GN=VLN2 PE=1 SV=2	[more]
VILI4_ARATH	2.049e-35	63.89	Villin-4 OS=Arabidopsis thaliana GN=VLN4 PE=1 SV=1	[more]
GELS_HOMAM	2.876e-23	55.56	Gelsolin, cytoplasmic OS=Homarus americanus PE=1 S...	[more]
GELS_DROME	5.195e-22	53.03	Gelsolin OS=Drosophila melanogaster GN=Gel PE=1 SV...	[more]
GELS_CHICK	1.936e-21	47.54	Gelsolin OS=Gallus gallus GN=GSN PE=2 SV=1	[more]
GELS1_LUMTE	4.320e-21	46.97	Gelsolin-like protein 1 OS=Lumbricus terrestris GN...	[more]
GELS_HORSE	5.550e-21	41.27	Gelsolin OS=Equus caballus GN=GSN PE=1 SV=2	[more]
GELS_HUMAN	7.211e-21	41.27	Gelsolin OS=Homo sapiens GN=GSN PE=1 SV=1	[more]
GELS_PIG	7.212e-21	41.27	Gelsolin (Fragment) OS=Sus scrofa GN=GSN PE=1 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-100-006-C02-CT.F Sweet orange leaf, greenhouse plant Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY650013 ID=EY650013; Name=EY650013; organism=Citrus sinensis; type=EST; length=936bp
TTCATTTTTCTCTCTCTATCTCTTTCGGGAGGGCGGGGGGCTGAAAAGAC
CCCCAACGAATTAAAAAAAAAAAAAAGTTAGAGAGAGAGCCCAGAACCCA
CAGCACACACACAACACAACCTCCTCAATTATTCACTTTTCTTTCTCTTT
TCCCCAGTATCTTTTATTTGCCAGCTTTTCCTTTTTTCGGAGTGCATCAT
CACTCTTCACACACACACACATTGAGATCCCTCTCTCTGGGTCTTTAAGT
GAATATTACAGTTTCGAGGTAGCAAAATCATCGAGTCTTTATTCTGTTTG
TGATTATAATAACAGTAATAAAGAAGGATTTTGAGGATGGCTACCTCTGC
AAAATCTTTGGATCCTGCATTTCAAGGAGCGGGTCAGAGAGTTGGTACTG
AAATTTGGCGAATTGAGAATTTTCAACCGGTTCCATTGCCTAAGTCTGAA
CATGGAAAATTCTATATGGGGGATTGCTACATCGTCTTGGAGACAACACC
TGGCAAGGGAGGGGCTTATCTGGATGACATACATTTCTGGATGGGAAAAG
AACAAGTAGGATGAANCTGGAACAGCAGCTATTAAAACTGTGGAACTTGA
TGCCTGTCTTGGAGGGCGGGCAGTTCAACACCGGGAACTTCAAGGGCGTG
GATCTGACAAATTTTTGGGCTATTTGAAGCGCTGGGTTATACACTGGGGG
GGGGGGGGGTGACTCGCGGGTGTAGAAAAACGGAGGGACAGAAATCGAAA
GAGGGGTGGCTCTCTGCAGGGGAAACGAGTCTGCAAAAAAAGCGAGGGCC
TTGTCGGTGGTCTGCACTGATCAAGAGGGAGGGGTGTTTCGGGGACCTAG
GGAGGGGTCTTGCGTTGAGGGGCGCAATGTCATGGCGGGAAGGGGCGGGG
CGGAGGAGGATGACCGTTCGGGGAGAAGCCCGGCGG