DY305547

Overview
NameDY305547
Unique NameDY305547
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length1087
Libraries
Library NameType
SLHcdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO1C_ORYSJ (Protein argonaute 1C OS=Oryza sativa subsp. japonica GN=AGO1C PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 5.151e-15
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  462 QVRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            +V G NTVL+D V  RIPLVTDRP IIFG DVTHP  GEDS+ SI+
Sbjct:  692 KVGGRNTVLVDAVSRRIPLVTDRPTIIFGADVTHPHPGEDSSPSIA 737

HSP 2 Score: 40.817 bits (94), Expect = 5.151e-15
Identity = 20/42 (47.62%), Postives = 30/42 (71.43%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRN-TRGVV 798
            VVAS DW E+  Y GLVSAQA+ +E+ + L+ ++++  RG V
Sbjct:  739 VVASQDWPEVTKYAGLVSAQAHRQELIEDLYKIWQDPQRGTV 780
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGOL_ARATH (Argonaute-like protein At2g27880 OS=Arabidopsis thaliana GN=At2g27880 PE=2 SV=1)

HSP 1 Score: 55.8398 bits (133), Expect = 5.153e-15
Identity = 28/43 (65.12%), Postives = 32/43 (74.42%), Query Frame = 3 
Query:  471 G*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            G NTVL D +R  IPL+TDRP II G DVTHP  GEDS+ SI+
Sbjct:  694 GRNTVLNDAIRRNIPLITDRPTIIMGADVTHPQPGEDSSPSIA 736

HSP 2 Score: 46.9802 bits (110), Expect = 5.153e-15
Identity = 25/42 (59.52%), Postives = 32/42 (76.19%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRN-TRGVV 798
            VVASMDW E+  Y+GLVSAQA+ EEI Q L+ L ++  RG+V
Sbjct:  738 VVASMDWPEINKYRGLVSAQAHREEIIQDLYKLVQDPQRGLV 779
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO1A_ORYSJ (Protein argonaute 1A OS=Oryza sativa subsp. japonica GN=AGO1A PE=2 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 6.682e-15
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  462 QVRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            +V G NTVL+D +  RIPLV+DRP IIFG DVTHP  GEDS+ SI+
Sbjct:  763 KVGGRNTVLVDALTRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIA 808

HSP 2 Score: 43.1282 bits (100), Expect = 6.682e-15
Identity = 22/42 (52.38%), Postives = 30/42 (71.43%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRN-TRGVV 798
            VVAS DW E+  Y GLVSAQA+ +E+ Q LF ++++  RG V
Sbjct:  810 VVASQDWPEVTKYAGLVSAQAHRQELIQDLFKVWKDPQRGTV 851
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO1B_ORYSJ (Protein argonaute 1B OS=Oryza sativa subsp. japonica GN=AGO1B PE=2 SV=3)

HSP 1 Score: 59.3066 bits (142), Expect = 8.676e-15
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  462 QVRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            +V G NTVL+D +  RIPLV+DRP IIFG DVTHP  GEDS+ SI+
Sbjct:  800 KVGGRNTVLVDALTRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIA 845

HSP 2 Score: 42.743 bits (99), Expect = 8.676e-15
Identity = 22/42 (52.38%), Postives = 30/42 (71.43%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRNT-RGVV 798
            VVAS DW E+  Y GLVSAQA+ +E+ Q LF ++++  RG V
Sbjct:  847 VVASQDWPEVTKYAGLVSAQAHRQELIQDLFKVWQDPHRGTV 888
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO1D_ORYSJ (Protein argonaute 1D OS=Oryza sativa subsp. japonica GN=AGO1D PE=2 SV=1)

HSP 1 Score: 62.003 bits (149), Expect = 1.467e-14
Identity = 32/46 (69.57%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  462 QVRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            +V G NTVL+D V  RIPLVTDRP IIFG DVTHP  GEDS+ SI+
Sbjct:  723 KVGGRNTVLVDAVSRRIPLVTDRPTIIFGADVTHPHPGEDSSPSIA 768

HSP 2 Score: 39.2762 bits (90), Expect = 1.467e-14
Identity = 17/33 (51.52%), Postives = 24/33 (72.73%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNL 774
            VVAS DW E+  Y GLVSAQ++ +E+   L+N+
Sbjct:  770 VVASQDWPEVTKYAGLVSAQSHRQELIDDLYNI 802
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO1_ARATH (Protein argonaute OS=Arabidopsis thaliana GN=AGO1 PE=1 SV=1)

HSP 1 Score: 59.3066 bits (142), Expect = 4.176e-14
Identity = 30/46 (65.22%), Postives = 36/46 (78.26%), Query Frame = 3 
Query:  462 QVRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            +V G NTVL+D +  RIPLV+DRP IIFG DVTHP  GEDS+ SI+
Sbjct:  730 KVGGRNTVLVDALSRRIPLVSDRPTIIFGADVTHPHPGEDSSPSIA 775

HSP 2 Score: 40.4318 bits (93), Expect = 4.176e-14
Identity = 21/42 (50.00%), Postives = 29/42 (69.05%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRN-TRGVV 798
            VVAS DW E+  Y GLV AQA+ +E+ Q LF  +++  +GVV
Sbjct:  777 VVASQDWPEITKYAGLVCAQAHRQELIQDLFKEWKDPQKGVV 818
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: PNH1_ORYSJ (Protein argonaute PNH1 OS=Oryza sativa subsp. japonica GN=PHN1 PE=2 SV=1)

HSP 1 Score: 56.225 bits (134), Expect = 1.251e-12
Identity = 28/43 (65.12%), Postives = 33/43 (76.74%), Query Frame = 3 
Query:  471 G*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            G NTVL+D +  RIPLV+D P IIFG DVTHP  GEDS+ SI+
Sbjct:  677 GRNTVLLDAISWRIPLVSDIPTIIFGADVTHPETGEDSSPSIA 719

HSP 2 Score: 38.5058 bits (88), Expect = 1.251e-12
Identity = 20/42 (47.62%), Postives = 27/42 (64.29%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRN-TRGVV 798
            VVAS DW E+  Y GLV AQA+ +E+ Q L+  + +  RG V
Sbjct:  721 VVASQDWPEVTKYAGLVCAQAHRQELIQDLYKTWHDPQRGTV 762
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: PINH_ARATH (Protein PINHEAD OS=Arabidopsis thaliana GN=At5g43810 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 2.738e-12
Identity = 27/43 (62.79%), Postives = 33/43 (76.74%), Query Frame = 3 
Query:  471 G*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            G NTVL+D +  RIPLV+D P IIFG DVTHP  GE+S+ SI+
Sbjct:  682 GRNTVLVDAISCRIPLVSDIPTIIFGADVTHPENGEESSPSIA 724

HSP 2 Score: 39.2762 bits (90), Expect = 2.738e-12
Identity = 20/42 (47.62%), Postives = 28/42 (66.67%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRN-TRGVV 798
            VVAS DW E+  Y GLV AQA+ +E+ Q L+  +++  RG V
Sbjct:  726 VVASQDWPEVTKYAGLVCAQAHRQELIQDLYKTWQDPVRGTV 767
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO12_ORYSJ (Protein argonaute 12 OS=Oryza sativa subsp. japonica GN=AGO12 PE=2 SV=2)

HSP 1 Score: 54.299 bits (129), Expect = 7.764e-12
Identity = 26/46 (56.52%), Postives = 35/46 (76.09%), Query Frame = 3 
Query:  462 QVRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            +V G NTVL D +  +IP++TDRP I+FG DVTHP  GED++ SI+
Sbjct:  747 KVGGRNTVLEDALHKKIPILTDRPTIVFGADVTHPSPGEDASPSIA 792

HSP 2 Score: 37.7354 bits (86), Expect = 7.764e-12
Identity = 18/31 (58.06%), Postives = 22/31 (70.97%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLF 768
            VVASMDW E+  YK LVS Q++ EEI   L+
Sbjct:  794 VVASMDWPEVTKYKCLVSTQSHREEIISNLY 824
[Close]
BLAST of DY305547 vs. ExPASy Swiss-Prot
Match: AGO11_ORYSJ (Protein argonaute 11 OS=Oryza sativa subsp. japonica GN=AGO11 PE=2 SV=2)

HSP 1 Score: 54.6842 bits (130), Expect = 1.705e-11
Identity = 28/45 (62.22%), Postives = 33/45 (73.33%), Query Frame = 3 
Query:  465 VRG*NTVLIDIVR*RIPLVTDRPAIIFGVDVTHP*LGEDSNLSIS 599
            V G NTVL D +  RIPL+TD P +IFG DVTHP  GEDS+ SI+
Sbjct:  581 VGGRNTVLEDALHRRIPLLTDMPTMIFGADVTHPPAGEDSSPSIA 625

HSP 2 Score: 36.1946 bits (82), Expect = 1.705e-11
Identity = 18/38 (47.37%), Postives = 26/38 (68.42%), Query Frame = 1 
Query:  676 VVASMDWLELIMYKGLVSAQAYHEEINQGLFNLFRNTR 789
            VVASMDW E+  YK  VS+Q++ EEI   LF   ++++
Sbjct:  627 VVASMDWPEVSKYKCSVSSQSHREEIIADLFTEVKDSQ 664
The following BLAST results are available for this feature:
BLAST of DY305547 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 10
Match NameE-valueIdentityDescription
AGO1C_ORYSJ5.151e-1569.57Protein argonaute 1C OS=Oryza sativa subsp. japoni... [more]
AGOL_ARATH5.153e-1565.12Argonaute-like protein At2g27880 OS=Arabidopsis th... [more]
AGO1A_ORYSJ6.682e-1565.22Protein argonaute 1A OS=Oryza sativa subsp. japoni... [more]
AGO1B_ORYSJ8.676e-1565.22Protein argonaute 1B OS=Oryza sativa subsp. japoni... [more]
AGO1D_ORYSJ1.467e-1469.57Protein argonaute 1D OS=Oryza sativa subsp. japoni... [more]
AGO1_ARATH4.176e-1465.22Protein argonaute OS=Arabidopsis thaliana GN=AGO1 ... [more]
PNH1_ORYSJ1.251e-1265.12Protein argonaute PNH1 OS=Oryza sativa subsp. japo... [more]
PINH_ARATH2.738e-1262.79Protein PINHEAD OS=Arabidopsis thaliana GN=At5g438... [more]
AGO12_ORYSJ7.764e-1256.52Protein argonaute 12 OS=Oryza sativa subsp. japoni... [more]
AGO11_ORYSJ1.705e-1162.22Protein argonaute 11 OS=Oryza sativa subsp. japoni... [more]
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Properties
Property NameValue
Genbank descriptionKN0AAM1BE12RM1 SLH Citrus sinensis cDNA 5, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>DY305547 ID=DY305547; Name=DY305547; organism=Citrus sinensis; type=EST; length=1087bp
ATTTAATACATGATTAAATTTTTATATCAGCATTGATTTTTTCAACATTA
TCGGCTTTCTACTTGCAGGGAGAATTAAGTGAGTTTGTGAAATGAAACTT
GGGATTGTTTCACAATGCTATTAGCCTAAGCATGCATCAAGGCTCAATAT
GCAGTATTTTGAAAATGTGGCTCTTAAAATCAATGCGAAGGTTGAGTTTA
CCCCTATTTTTGTACCTGTTTAATATTCTTGTTAATATGGTGAGAACAAA
TATAAGGGTTAATGAGAAAACATATAGGAGATGATTTCTTGTTGCAATTT
AATTTAAGACTTGATTTGCTGGAGTTACTGTAATTTTTTTTCAAATAATT
AAATTTTAATGTTCAAGATTTTTTTTTAAGTTGGGAGTGTCAAATTTCTT
GTTAAACTGATAGGTTTGTTTTCTTGGTAATGAAGATTAAGTTTGCTATT
TGTAATTTTATCAAGTTAGGGGATGAAACACTGTGCTCATTGATATTGTT
CGGTAAAGAATTCCTCTTGTGACTGATAGACCTGCAATTATATTTGGTGT
GGATGTCACCCATCCATAACTAGGGGAAGACTCTAATCTTTCAATAAGTG
TCTATATGTACACTCATATTCTATTTCTGTGTCCTGATAATTCACTCTGT
TAAAAGATTGAATTGACTGAAATAGGTGGTGGCCTCAATGGATTGGCTAG
AATTAATCATGTACAAAGGACTTGTCTCTGCTCAGGCCTACCATGAGGAA
ATTAATCAAGGTCTCTTTAATCTATTCAGGAATACAAGAGGGGTAGTATT
CCGGAAGAATGATCAGGGAGGATGATCCCCCCCCACAGCTCACTAAATCT
GAAATGATAAAATGGGCCAATTTAAGGAATCANGAAAAGATCTTTTTGTG
GAATCCCCCCAAATCTACCCAANNNGAAAAAAACCCCTTTCCCANNAACT
TATTNTACAGAAGGCCCNAACCCCCTCTCCCCAAAAGTTTTTNNTTNAAN
ANNNNTCTTAAAAAAAANNGGCGGGCAATTTGGGNNTTGTANTTTTTTNN
AATCACNNGAAAAAAAAANCCCNTTAAAATTGNTNCC
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