CN185479 | Citrus Genome Database

Overview


Name	CN185479
Unique Name	CN185479
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	577

Libraries


Library Name	Type
Washington Navel Orange Stored Fruit Pulp cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
CN185479_ssr300	microsatellite	CN185479_ssr300:300..314.	BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig760	contig	Csv1_Contig760:78..651.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJH_CUCSA (DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 160.614 bits (405), Expect = 2.603e-62
Identity = 81/94 (86.17%), Postives = 84/94 (89.36%), Query Frame = 3

Query:   54 MFGRAPKKSDNPKYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            MFGR PKKSDN KYYEILGVSKNASQDDLKKAYR+AAI+NHPDKGGDPEKFKELAQAYEVLSDP+KREIYDQYGEDALKE        HDPFDI
Sbjct:    1 MFGR-PKKSDNTKYYEILGVSKNASQDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYGEDALKE-GMGGGGGHDPFDI 92

HSP 2 Score: 98.5969 bits (244), Expect = 2.603e-62
Identity = 45/56 (80.36%), Postives = 51/56 (91.07%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTVS 576
            DV+HPL+ S+EDLY GTSKKLSLSRN +C+KC+GKGSKSGASMKC GCQGSGM VS
Sbjct:  118 DVIHPLKVSLEDLYNGTSKKLSLSRNVICSKCKGKGSKSGASMKCPGCQGSGMKVS 173

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJH2_ALLPO (DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE=2 SV=1)

HSP 1 Score: 159.073 bits (401), Expect = 6.285e-61
Identity = 73/94 (77.66%), Postives = 82/94 (87.23%), Query Frame = 3

Query:   54 MFGRAPKKSDNPKYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            MFGRAPKKSDN KYYE+LGVSKNA+ +DLKKAYR+AAI+NHPDKGGDPEKFKE+ QAYEVL+DP+KREIYDQYGE+ LKE        HDPFDI
Sbjct:    1 MFGRAPKKSDNTKYYEVLGVSKNATPEDLKKAYRKAAIKNHPDKGGDPEKFKEIGQAYEVLNDPEKREIYDQYGEEGLKEGMGGGGGVHDPFDI 94

HSP 2 Score: 95.5153 bits (236), Expect = 6.285e-61
Identity = 44/56 (78.57%), Postives = 50/56 (89.29%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTVS 576
            DV+HPL+ S+EDLY GTSKKLSLSRN +CTKC+GKGSKSGASM C+ CQGSGM VS
Sbjct:  120 DVVHPLKVSLEDLYNGTSKKLSLSRNVLCTKCKGKGSKSGASMNCASCQGSGMKVS 175

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJH_ATRNU (DnaJ protein homolog ANJ1 OS=Atriplex nummularia PE=2 SV=1)

HSP 1 Score: 156.762 bits (395), Expect = 5.249e-60
Identity = 74/94 (78.72%), Postives = 82/94 (87.23%), Query Frame = 3

Query:   54 MFGRAPKKSDNPKYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            MFGRAPKKSD+ +YYEILGV K+AS +DLKKAY++AAI+NHPDKGGDPEKFKELA AYEVLSDP+KREIYDQYGEDALKE        HDPFDI
Sbjct:    1 MFGRAPKKSDSTRYYEILGVPKDASPEDLKKAYKKAAIKNHPDKGGDPEKFKELAHAYEVLSDPEKREIYDQYGEDALKEGMGGGGGMHDPFDI 94

HSP 2 Score: 94.7449 bits (234), Expect = 5.249e-60
Identity = 43/56 (76.79%), Postives = 51/56 (91.07%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTVS 576
            DV+HPL+ S+EDL+ GT+KKLSLSRN +C+KC GKGSKSGASMKCSGCQG+GM VS
Sbjct:  120 DVVHPLKVSLEDLFTGTTKKLSLSRNVICSKCTGKGSKSGASMKCSGCQGTGMKVS 175

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ3_ARATH (Chaperone protein dnaJ 3 OS=Arabidopsis thaliana GN=ATJ3 PE=1 SV=2)

HSP 1 Score: 154.451 bits (389), Expect = 7.451e-59
Identity = 75/95 (78.95%), Postives = 82/95 (86.32%), Query Frame = 3

Query:   54 MFGRAP-KKSDNPKYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            MFGR P KKSDN K+YEILGV K+AS +DLKKAY++AAI+NHPDKGGDPEKFKELAQAYEVLSDP+KREIYDQYGEDALKE        HDPFDI
Sbjct:    1 MFGRGPSKKSDNTKFYEILGVPKSASPEDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYGEDALKEGMGGGGGGHDPFDI 95

HSP 2 Score: 93.2041 bits (230), Expect = 7.451e-59
Identity = 42/56 (75.00%), Postives = 49/56 (87.50%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTVS 576
            DV+HPL+ S+ED+Y GT KKLSLSRN +C+KC GKGSKSGAS+KC GCQGSGM VS
Sbjct:  120 DVVHPLKVSLEDVYLGTMKKLSLSRNALCSKCNGKGSKSGASLKCGGCQGSGMKVS 175

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNAJ2_ARATH (Chaperone protein dnaJ 2 OS=Arabidopsis thaliana GN=ATJ2 PE=1 SV=2)

HSP 1 Score: 151.754 bits (382), Expect = 8.110e-58
Identity = 73/95 (76.84%), Postives = 81/95 (85.26%), Query Frame = 3

Query:   54 MFGRAP-KKSDNPKYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            MFGR P +KSDN K+YEILGV K A+ +DLKKAY++AAI+NHPDKGGDPEKFKELAQAYEVLSDP+KREIYDQYGEDALKE        HDPFDI
Sbjct:    1 MFGRGPSRKSDNTKFYEILGVPKTAAPEDLKKAYKKAAIKNHPDKGGDPEKFKELAQAYEVLSDPEKREIYDQYGEDALKEGMGGGGGGHDPFDI 95

HSP 2 Score: 92.4337 bits (228), Expect = 8.110e-58
Identity = 41/56 (73.21%), Postives = 49/56 (87.50%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTVS 576
            DV+HPL+ S+ED+Y GT+KKLSLSR  +C+KC GKGSKSGASMKC GCQGSGM +S
Sbjct:  121 DVVHPLKVSLEDVYLGTTKKLSLSRKALCSKCNGKGSKSGASMKCGGCQGSGMKIS 176

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJH1_ALLPO (DnaJ protein homolog 1 (Fragment) OS=Allium porrum GN=DNAJ1 PE=2 SV=1)

HSP 1 Score: 126.716 bits (317), Expect = 1.458e-50
Identity = 60/73 (82.19%), Postives = 64/73 (87.67%), Query Frame = 3

Query:  117 KNASQDDLKKAYREAAIQNHPDKGGDPEKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            KNAS DDLKKAYR+AAI+NHPDKGGDPEKFKELAQAY+VLSDP+KREIYDQYGEDALKE        HDPFDI
Sbjct:    1 KNASPDDLKKAYRKAAIKNHPDKGGDPEKFKELAQAYDVLSDPEKREIYDQYGEDALKEGMGGGGGDHDPFDI 73

HSP 2 Score: 93.2041 bits (230), Expect = 1.458e-50
Identity = 42/56 (75.00%), Postives = 50/56 (89.29%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTVS 576
            DV+HPL+ S+E+LY GTSKKLSLSRN +C+KC GKGSKSGASM+C+ CQGSGM VS
Sbjct:   99 DVVHPLKVSLEELYNGTSKKLSLSRNVICSKCNGKGSKSGASMRCASCQGSGMKVS 154

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJA4_MOUSE (DnaJ homolog subfamily A member 4 OS=Mus musculus GN=Dnaja4 PE=2 SV=1)

HSP 1 Score: 92.8189 bits (229), Expect = 5.001e-31
Identity = 46/83 (55.42%), Postives = 60/83 (72.29%), Query Frame = 3

Query:   90 KYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            +YY+ILGV  +AS +++KKAYR+ A++ HPDK  D  EKFK ++QAYEVLSDP+KR+IYDQ GE A+KE          P DI
Sbjct:    6 QYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGEKFKLISQAYEVLSDPKKRDIYDQGGEQAIKEGGSGSPSFSSPMDI 88

HSP 2 Score: 61.6178 bits (148), Expect = 5.001e-31
Identity = 27/55 (49.09%), Postives = 37/55 (67.27%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTV 573
            +V+H L  ++EDLY G +KKL+L +N +C KC G G K G+  KC  C+G GM V
Sbjct:  107 NVVHQLSVTLEDLYNGITKKLALQKNVICEKCEGIGGKKGSVEKCPLCKGRGMQV 161

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJA4_HUMAN (DnaJ homolog subfamily A member 4 OS=Homo sapiens GN=DNAJA4 PE=1 SV=1)

HSP 1 Score: 92.4337 bits (228), Expect = 5.001e-31
Identity = 45/83 (54.22%), Postives = 60/83 (72.29%), Query Frame = 3

Query:   90 KYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKEXXXXXXXAHDPFDI 335
            +YY+ILGV  +AS +++KKAYR+ A++ HPDK  D  EKFK ++QAYEVLSDP+KR++YDQ GE A+KE          P DI
Sbjct:    6 QYYDILGVKPSASPEEIKKAYRKLALKYHPDKNPDEGEKFKLISQAYEVLSDPKKRDVYDQGGEQAIKEGGSGSPSFSSPMDI 88

HSP 2 Score: 62.003 bits (149), Expect = 5.001e-31
Identity = 26/55 (47.27%), Postives = 37/55 (67.27%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTV 573
            +V+H L  ++EDLY G +KKL+L +N +C KC G G K G+  KC  C+G GM +
Sbjct:  107 NVVHQLSVTLEDLYNGVTKKLALQKNVICEKCEGVGGKKGSVEKCPLCKGRGMQI 161

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJA2_RAT (DnaJ homolog subfamily A member 2 OS=Rattus norvegicus GN=Dnaja2 PE=2 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.509e-31
Identity = 40/69 (57.97%), Postives = 55/69 (79.71%), Query Frame = 3

Query:   90 KYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            K Y+ILGV   AS+++LKKAYR+ A + HPDK  +  +KFKE++ AYEVLS+P+KRE+YD+YGE  L+E
Sbjct:    8 KLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLRE 76

HSP 2 Score: 70.0922 bits (170), Expect = 6.509e-31
Identity = 29/55 (52.73%), Postives = 41/55 (74.55%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTV 573
            D++HPL+ S+EDLY G + KL LS+N +C+ C G+G KSGA  KCS C+G G+ +
Sbjct:  115 DMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRI 169

[Close]

BLAST of CN185479 vs. ExPASy Swiss-Prot
Match: DNJA2_MOUSE (DnaJ homolog subfamily A member 2 OS=Mus musculus GN=Dnaja2 PE=1 SV=1)

HSP 1 Score: 83.9593 bits (206), Expect = 6.509e-31
Identity = 40/69 (57.97%), Postives = 55/69 (79.71%), Query Frame = 3

Query:   90 KYYEILGVSKNASQDDLKKAYREAAIQNHPDKGGDP-EKFKELAQAYEVLSDPQKREIYDQYGEDALKE 293
            K Y+ILGV   AS+++LKKAYR+ A + HPDK  +  +KFKE++ AYEVLS+P+KRE+YD+YGE  L+E
Sbjct:    8 KLYDILGVPPGASENELKKAYRKLAKEYHPDKNPNAGDKFKEISFAYEVLSNPEKRELYDRYGEQGLRE 76

HSP 2 Score: 70.0922 bits (170), Expect = 6.509e-31
Identity = 29/55 (52.73%), Postives = 41/55 (74.55%), Query Frame = 1

Query:  409 DVLHPLEGSVEDLYKGTSKKLSLSRNGVCTKCRGKGSKSGASMKCSGCQGSGMTV 573
            D++HPL+ S+EDLY G + KL LS+N +C+ C G+G KSGA  KCS C+G G+ +
Sbjct:  115 DMMHPLKVSLEDLYNGKTTKLQLSKNVLCSACSGQGGKSGAVQKCSACRGRGVRI 169

The following BLAST results are available for this feature:

BLAST of CN185479 vs. ExPASy Swiss-Prot

BLAST of CN185479 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500


Match Name	E-value	Identity	Description
DNJH_CUCSA	2.603e-62	86.17	DnaJ protein homolog OS=Cucumis sativus GN=DNAJ1 P...	[more]
DNJH2_ALLPO	6.285e-61	77.66	DnaJ protein homolog 2 OS=Allium porrum GN=LDJ2 PE...	[more]
DNJH_ATRNU	5.249e-60	78.72	DnaJ protein homolog ANJ1 OS=Atriplex nummularia P...	[more]
DNAJ3_ARATH	7.451e-59	78.95	Chaperone protein dnaJ 3 OS=Arabidopsis thaliana G...	[more]
DNAJ2_ARATH	8.110e-58	76.84	Chaperone protein dnaJ 2 OS=Arabidopsis thaliana G...	[more]
DNJH1_ALLPO	1.458e-50	82.19	DnaJ protein homolog 1 (Fragment) OS=Allium porrum...	[more]
DNJA4_MOUSE	5.001e-31	55.42	DnaJ homolog subfamily A member 4 OS=Mus musculus ...	[more]
DNJA4_HUMAN	5.001e-31	54.22	DnaJ homolog subfamily A member 4 OS=Homo sapiens ...	[more]
DNJA2_RAT	6.509e-31	57.97	DnaJ homolog subfamily A member 2 OS=Rattus norveg...	[more]
DNJA2_MOUSE	6.509e-31	57.97	DnaJ homolog subfamily A member 2 OS=Mus musculus ...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS05_0001G11_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0001G11, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN185479 ID=CN185479; Name=CN185479; organism=Citrus sinensis; type=EST; length=577bp
AGTGCCGTCTCGCGTCTACTCTGAAGATCTGAAATTTTTCTAGATACAAG
AAGATGTTCGGAAGAGCGCCCAAGAAAAGCGATAACCCAAAGTACTATGA
GATTCTCGGAGTCTCGAAAAACGCTTCGCAAGATGATCTGAAGAAGGCTT
ATAGGGAAGCCGCCATCCAAAACCATCCTGACAAGGGCGGTGATCCTGAG
AAGTTTAAAGAGTTGGCGCAAGCGTATGAGGTTCTGAGTGACCCCCAGAA
GCGTGAGATATATGATCAATACGGCGAGGATGCGCTTAAAGAGGGAATGG
GTGGTGGTGGTGGTGCCCATGACCCATTCGACATATTCCAATCCTTCTTC
CGTGGCAGCCCATTTGGGGGTGGGAGCAGCAGAGGAGAGAAGGCGAAGGA
GGGGAGAGGACGTACTCCATCCCCTCGAGGGTTCGGTGGAGGATCTGTAC
AAGGGGACATCTAAGAAGCTCTCGCTCTCCCGCAATGGAGTCTGCACCAA
GTGCAGAGGGAAGGGGTCTAAGTCAGGGGCATCGATGAAATGTTCTGGGT
GCCAGGGATCTGGAATGACAGTTTCAA