CN185141

Overview
NameCN185141
Unique NameCN185141
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length524
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK_BRARP (S-adenosylmethionine synthetase OS=Brassica rapa subsp. pekinensis GN=SAMS PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 3.191e-72
Identity = 126/130 (96.92%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 130

HSP 2 Score: 28.1054 bits (61), Expect = 3.191e-72
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET E MPLSHVL
Sbjct:  131 TDETPELMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK5_BRAJU (S-adenosylmethionine synthetase 5 OS=Brassica juncea GN=MSAMS5 PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 3.191e-72
Identity = 126/130 (96.92%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 130

HSP 2 Score: 28.1054 bits (61), Expect = 3.191e-72
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET E MPLSHVL
Sbjct:  131 TDETPELMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK3_BRAJU (S-adenosylmethionine synthetase 3 OS=Brassica juncea GN=MSAMS3 PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 3.191e-72
Identity = 126/130 (96.92%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 130

HSP 2 Score: 28.1054 bits (61), Expect = 3.191e-72
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET E MPLSHVL
Sbjct:  131 TDETPELMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK1_BRAJU (S-adenosylmethionine synthetase 1 OS=Brassica juncea GN=SAMS1 PE=2 SV=1)

HSP 1 Score: 264.618 bits (675), Expect = 3.191e-72
Identity = 126/130 (96.92%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 130

HSP 2 Score: 28.1054 bits (61), Expect = 3.191e-72
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET E MPLSHVL
Sbjct:  131 TDETPELMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK2_ARATH (S-adenosylmethionine synthetase 2 OS=Arabidopsis thaliana GN=SAM2 PE=1 SV=1)

HSP 1 Score: 264.233 bits (674), Expect = 4.162e-72
Identity = 125/130 (96.15%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA +DYEKIVRDTCRSIGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATIDYEKIVRDTCRSIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 130

HSP 2 Score: 28.1054 bits (61), Expect = 4.162e-72
Identity = 12/14 (85.71%), Postives = 12/14 (85.71%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET E MPLSHVL
Sbjct:  131 TDETPELMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK1_CATRO (S-adenosylmethionine synthetase 1 OS=Catharanthus roseus GN=SAMS1 PE=1 SV=1)

HSP 1 Score: 261.922 bits (668), Expect = 4.162e-72
Identity = 125/130 (96.15%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPE+IGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKATVDYEKIVRDTCRSIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEEIGAGDQGHMFGYA 130

HSP 2 Score: 30.4166 bits (67), Expect = 4.162e-72
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET EFMPLSHVL
Sbjct:  131 TDETPEFMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK2_PEA (S-adenosylmethionine synthetase 2 OS=Pisum sativum GN=SAMS2 PE=2 SV=1)

HSP 1 Score: 261.922 bits (668), Expect = 4.163e-72
Identity = 125/129 (96.90%), Postives = 127/129 (98.45%), Query Frame = 3 
Query:   90 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR+IGFVSDDVGLDAD+CKVLVNIEQQSPDIAQGVHGH TKRPEDIGAGDQGHMFGYA
Sbjct:    4 ETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRNIGFVSDDVGLDADNCKVLVNIEQQSPDIAQGVHGHLTKRPEDIGAGDQGHMFGYA 132

HSP 2 Score: 30.4166 bits (67), Expect = 4.163e-72
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET EFMPLSHVL
Sbjct:  133 TDETPEFMPLSHVL 146
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK_CARPA (S-adenosylmethionine synthetase OS=Carica papaya GN=SAMS PE=2 SV=1)

HSP 1 Score: 261.151 bits (666), Expect = 7.073e-72
Identity = 125/130 (96.15%), Postives = 127/130 (97.69%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACL QDP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCRSIGFVSDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLAQDPDSKVACETCTKTNMVMVFGEITTKADVDYEKIVRDTCRSIGFVSDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYA 130

HSP 2 Score: 30.4166 bits (67), Expect = 7.073e-72
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET EFMPLSHVL
Sbjct:  131 TDETPEFMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK1_POPTR (S-adenosylmethionine synthetase 1 OS=Populus trichocarpa GN=METK1 PE=2 SV=1)

HSP 1 Score: 262.307 bits (669), Expect = 7.078e-72
Identity = 123/130 (94.62%), Postives = 128/130 (98.46%), Query Frame = 3 
Query:   87 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDP+SKVACETCTKTNMVMVFGEITTK K+DYEKIVRDTCR+IGF+SDDVGLDAD CKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYA
Sbjct:    1 METFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPDSKVACETCTKTNMVMVFGEITTKGKIDYEKIVRDTCRNIGFISDDVGLDADKCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYA 130

HSP 2 Score: 29.261 bits (64), Expect = 7.078e-72
Identity = 12/14 (85.71%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET E+MPLSHVL
Sbjct:  131 TDETPEYMPLSHVL 144
[Close]
BLAST of CN185141 vs. ExPASy Swiss-Prot
Match: METK4_HORVU (S-adenosylmethionine synthetase 4 OS=Hordeum vulgare GN=SAM4 PE=2 SV=1)

HSP 1 Score: 259.225 bits (661), Expect = 2.670e-71
Identity = 122/131 (93.13%), Postives = 128/131 (97.71%), Query Frame = 3 
Query:   84 KMETFLFTSESVNEGHPDKLCDQISDAVLDACLEQDPESKVACETCTKTNMVMVFGEITTKAKVDYEKIVRDTCRSIGFVSDDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEDIGAGDQGHMFGYA 476
            ++ETFLFTSESVNEGHPDKLCDQISDAVLDACL +DP+SKVACETCTKTNMVMVFGEITTKA VDYEKIVRDTCR IGFVS+DVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPE+IGAGDQGHMFGYA
Sbjct:    3 EVETFLFTSESVNEGHPDKLCDQISDAVLDACLAEDPDSKVACETCTKTNMVMVFGEITTKANVDYEKIVRDTCRGIGFVSNDVGLDADHCKVLVNIEQQSPDIAQGVHGHFTKRPEEIGAGDQGHMFGYA 133

HSP 2 Score: 30.4166 bits (67), Expect = 2.670e-71
Identity = 13/14 (92.86%), Postives = 13/14 (92.86%), Query Frame = 1 
Query:  478 TDETSEFMPLSHVL 519
            TDET EFMPLSHVL
Sbjct:  134 TDETPEFMPLSHVL 147
The following BLAST results are available for this feature:
BLAST of CN185141 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 500
Match NameE-valueIdentityDescription
METK_BRARP3.191e-7296.92S-adenosylmethionine synthetase OS=Brassica rapa s... [more]
METK5_BRAJU3.191e-7296.92S-adenosylmethionine synthetase 5 OS=Brassica junc... [more]
METK3_BRAJU3.191e-7296.92S-adenosylmethionine synthetase 3 OS=Brassica junc... [more]
METK1_BRAJU3.191e-7296.92S-adenosylmethionine synthetase 1 OS=Brassica junc... [more]
METK2_ARATH4.162e-7296.15S-adenosylmethionine synthetase 2 OS=Arabidopsis t... [more]
METK1_CATRO4.162e-7296.15S-adenosylmethionine synthetase 1 OS=Catharanthus ... [more]
METK2_PEA4.163e-7296.90S-adenosylmethionine synthetase 2 OS=Pisum sativum... [more]
METK_CARPA7.073e-7296.15S-adenosylmethionine synthetase OS=Carica papaya G... [more]
METK1_POPTR7.078e-7294.62S-adenosylmethionine synthetase 1 OS=Populus trich... [more]
METK4_HORVU2.670e-7193.13S-adenosylmethionine synthetase 4 OS=Hordeum vulga... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0006N06_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006N06, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN185141 ID=CN185141; Name=CN185141; organism=Citrus sinensis; type=EST; length=524bp
GAGGGTTCCGCTTTGCTATTGAGGTCTGGTCTTAAGTCTGAATCTTAGAT
CTGCGCCGAAACACTTGCCAGCCACAGCTTGATAAGATGGAGACCTTTCT
ATTCACCTCTGAATCTGTGAACGAGGGTCACCCCGACAAGTTGTGCGACC
AGATCTCTGATGCGGTGCTTGACGCCTGCCTTGAGCAGGATCCTGAGAGC
AAGGTTGCCTGTGAGACATGCACCAAGACCAACATGGTCATGGTCTTTGG
AGAGATTACCACCAAGGCCAAGGTAGACTATGAGAAGATTGTTCGCGACA
CTTGCCGTTCCATTGGATTTGTTTCTGATGATGTGGGTCTTGACGCCGAT
CACTGCAAAGTCCTGGTCAATATTGAGCAACAAAGCCCGGATATTGCCCA
GGGTGTTCATGGCCACTTTACAAAGCGCCCTGAGGACATTGGTGCTGGTG
ACCAGGGCCATATGTTTGGTTATGCCTACTGATGAGACCTCTGAATTTAT
GCCTTTGAGCCATGTCCTTTGCCA
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