CN184794 | Citrus Genome Database

Overview


Name	CN184794
Unique Name	CN184794
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	381

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig12605	contig	Csv1_Contig12605:489..867.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_MAIZE (Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2 SV=2)

HSP 1 Score: 175.637 bits (444), Expect = 5.946e-55
Identity = 83/92 (90.22%), Postives = 87/92 (94.57%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP KN+TCLTRLDHNRALGQISE+LNVQVSDVKNVIIWGNHSSSQYPDVNHATV T+ GEKPVRELV DD WLNGEFITTVQQRGA
Sbjct:  142 KEFAPSIPEKNVTCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSSQYPDVNHATVKTSTGEKPVRELVSDDEWLNGEFITTVQQRGA 233

HSP 2 Score: 58.5362 bits (140), Expect = 5.946e-55
Identity = 29/35 (82.86%), Postives = 29/35 (82.86%), Query Frame = -1

Query:  265 SALEQHAAPNCKVLVVANPANTNALILKEICAINP 369
            SALE HAAPNCKVLVVANPANTNALILKE     P
Sbjct:  115 SALEAHAAPNCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_MEDSA (Malate dehydrogenase, cytoplasmic OS=Medicago sativa GN=CMDH PE=2 SV=1)

HSP 1 Score: 174.481 bits (441), Expect = 6.466e-54
Identity = 82/92 (89.13%), Postives = 89/92 (96.74%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP +NI+CLTRLDHNRALGQISE+LNVQVSDVKNVIIWGNHSS+QYPDVNHATVNT AGEKPVR+LV DDAWLNGEFI+TVQQRGA
Sbjct:  142 KEFAPSIPERNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVNTPAGEKPVRQLVSDDAWLNGEFISTVQQRGA 233

HSP 2 Score: 56.225 bits (134), Expect = 6.466e-54
Identity = 28/35 (80.00%), Postives = 29/35 (82.86%), Query Frame = -1

Query:  265 SALEQHAAPNCKVLVVANPANTNALILKEICAINP 369
            SALE+HAA NCKVLVVANPANTNALILKE     P
Sbjct:  115 SALEKHAAANCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_MESCR (Malate dehydrogenase, cytoplasmic OS=Mesembryanthemum crystallinum GN=MDH PE=2 SV=1)

HSP 1 Score: 169.474 bits (428), Expect = 6.466e-54
Identity = 81/92 (88.04%), Postives = 86/92 (93.48%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP KNI+CLTRLDHNRALGQISE+LNVQVSDVKNVIIWGNHSS+QYPDVNHATV T   +KPVRELV DDAWLNGEFITTVQQRGA
Sbjct:  142 KEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVIIWGNHSSTQYPDVNHATVKTQGVDKPVRELVADDAWLNGEFITTVQQRGA 233

HSP 2 Score: 61.2326 bits (147), Expect = 6.466e-54
Identity = 30/39 (76.92%), Postives = 32/39 (82.05%), Query Frame = -1

Query:  265 RLRLSALEQHAAPNCKVLVVANPANTNALILKEICAINP 381
            + + SALEQHAAPNCKVLVVANPANTNALILKE     P
Sbjct:  111 KAQASALEQHAAPNCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC2_ARATH (Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis thaliana GN=At5g43330 PE=2 SV=1)

HSP 1 Score: 168.703 bits (426), Expect = 4.137e-53
Identity = 79/92 (85.87%), Postives = 87/92 (94.57%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP KNITCLTRLDHNRALGQ+SE+L+V VSDVKNVIIWGNHSS+QYPDVNHATV T+ GEKPVRELVK+D WLNGEFI+TVQQRGA
Sbjct:  142 KEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNVIIWGNHSSTQYPDVNHATVKTSVGEKPVRELVKNDEWLNGEFISTVQQRGA 233

HSP 2 Score: 59.3066 bits (142), Expect = 4.137e-53
Identity = 29/35 (82.86%), Postives = 30/35 (85.71%), Query Frame = -1

Query:  265 SALEQHAAPNCKVLVVANPANTNALILKEICAINP 369
            SALE+HAAPNCKVLVVANPANTNALILKE     P
Sbjct:  115 SALEKHAAPNCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC1_ARATH (Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis thaliana GN=At1g04410 PE=1 SV=2)

HSP 1 Score: 168.703 bits (426), Expect = 9.163e-53
Identity = 80/92 (86.96%), Postives = 88/92 (95.65%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP KNI+CLTRLDHNRALGQISE+L+V VSDVKNVIIWGNHSSSQYPDVNHA V T++GEKPVRELVKDDAWL+GEFI+TVQQRGA
Sbjct:  142 KEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNVIIWGNHSSSQYPDVNHAKVQTSSGEKPVRELVKDDAWLDGEFISTVQQRGA 233

HSP 2 Score: 58.151 bits (139), Expect = 9.163e-53
Identity = 28/35 (80.00%), Postives = 30/35 (85.71%), Query Frame = -1

Query:  265 SALEQHAAPNCKVLVVANPANTNALILKEICAINP 369
            +ALE+HAAPNCKVLVVANPANTNALILKE     P
Sbjct:  115 AALEKHAAPNCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_ORYSJ (Malate dehydrogenase, cytoplasmic OS=Oryza sativa subsp. japonica GN=Os10g0478200 PE=1 SV=3)

HSP 1 Score: 167.933 bits (424), Expect = 1.194e-52
Identity = 80/92 (86.96%), Postives = 85/92 (92.39%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP KNITCLTRLDHNRALGQISEKLNVQV+DVKN IIWGNHSS+QYPDVNHATV T +GEKPVRELV DD WLN EFI+TVQQRGA
Sbjct:  142 KEFAPSIPEKNITCLTRLDHNRALGQISEKLNVQVTDVKNAIIWGNHSSTQYPDVNHATVKTPSGEKPVRELVADDEWLNTEFISTVQQRGA 233

HSP 2 Score: 58.5362 bits (140), Expect = 1.194e-52
Identity = 29/35 (82.86%), Postives = 29/35 (82.86%), Query Frame = -1

Query:  265 SALEQHAAPNCKVLVVANPANTNALILKEICAINP 369
            SALE HAAPNCKVLVVANPANTNALILKE     P
Sbjct:  115 SALEAHAAPNCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_BETVU (Malate dehydrogenase, cytoplasmic OS=Beta vulgaris GN=NR1 PE=1 SV=1)

HSP 1 Score: 167.548 bits (423), Expect = 1.557e-52
Identity = 81/92 (88.04%), Postives = 84/92 (91.30%), Query Frame = -2

Query:   12 KKFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 287
            K+FAPSIP KNITCLTRLDHNRALGQISE+LN QVSDVKNVIIWGNHSSSQYPDVN  TV T +GEK VRELV DDAWLNGEFITTVQQRGA
Sbjct:  142 KEFAPSIPEKNITCLTRLDHNRALGQISERLNAQVSDVKNVIIWGNHSSSQYPDVNPCTVKTGSGEKAVRELVADDAWLNGEFITTVQQRGA 233

HSP 2 Score: 58.5362 bits (140), Expect = 1.557e-52
Identity = 29/35 (82.86%), Postives = 30/35 (85.71%), Query Frame = -1

Query:  265 SALEQHAAPNCKVLVVANPANTNALILKEICAINP 369
            SALEQ+AAPNCKVLVVANPANTNALILKE     P
Sbjct:  115 SALEQYAAPNCKVLVVANPANTNALILKEFAPSIP 149

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_PIG (Malate dehydrogenase, cytoplasmic OS=Sus scrofa GN=MDH1 PE=1 SV=4)

HSP 1 Score: 133.265 bits (334), Expect = 1.344e-34
Identity = 66/91 (72.53%), Postives = 71/91 (78.02%), Query Frame = -2

Query:   12 KFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 284
            K APSIP +N +CLTRLDHNRA  QI+ KL V   DVKNVIIWGNHSS+QYPDVNHA V   A E  V E VKDD+WL GEFITTVQQRGA
Sbjct:  142 KSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVIIWGNHSSTQYPDVNHAKVKLQAKEVGVYEAVKDDSWLKGEFITTVQQRGA 232

HSP 2 Score: 32.7278 bits (73), Expect = 1.344e-34
Identity = 14/25 (56.00%), Postives = 20/25 (80.00%), Query Frame = -1

Query:  295 SALEQHAAPNCKVLVVANPANTNAL 369
            +AL+++A  + KV+VV NPANTN L
Sbjct:  114 AALDKYAKKSVKVIVVGNPANTNCL 138

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_RAT (Malate dehydrogenase, cytoplasmic OS=Rattus norvegicus GN=Mdh1 PE=1 SV=3)

HSP 1 Score: 131.339 bits (329), Expect = 2.281e-34
Identity = 64/91 (70.33%), Postives = 70/91 (76.92%), Query Frame = -2

Query:   12 KFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 284
            K APSIP +N +CLTRLDHNRA  QI+ KL V   DVKNVIIWGNHSS+QYPDVNHA V     E  V E +KDD+WL GEFITTVQQRGA
Sbjct:  142 KSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFITTVQQRGA 232

HSP 2 Score: 33.8834 bits (76), Expect = 2.281e-34
Identity = 15/25 (60.00%), Postives = 20/25 (80.00%), Query Frame = -1

Query:  295 SALEQHAAPNCKVLVVANPANTNAL 369
            +ALE++A  + KV+VV NPANTN L
Sbjct:  114 AALEKYAKKSVKVIVVGNPANTNCL 138

[Close]

BLAST of CN184794 vs. ExPASy Swiss-Prot
Match: MDHC_MOUSE (Malate dehydrogenase, cytoplasmic OS=Mus musculus GN=Mdh1 PE=1 SV=3)

HSP 1 Score: 131.339 bits (329), Expect = 2.281e-34
Identity = 64/91 (70.33%), Postives = 70/91 (76.92%), Query Frame = -2

Query:   12 KFAPSIPAKNITCLTRLDHNRALGQISEKLNVQVSDVKNVIIWGNHSSSQYPDVNHATVNTAAGEKPVRELVKDDAWLNGEFITTVQQRGA 284
            K APSIP +N +CLTRLDHNRA  QI+ KL V   DVKNVIIWGNHSS+QYPDVNHA V     E  V E +KDD+WL GEFITTVQQRGA
Sbjct:  142 KSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVIIWGNHSSTQYPDVNHAKVKLQGKEVGVYEALKDDSWLKGEFITTVQQRGA 232

HSP 2 Score: 33.8834 bits (76), Expect = 2.281e-34
Identity = 15/25 (60.00%), Postives = 20/25 (80.00%), Query Frame = -1

Query:  295 SALEQHAAPNCKVLVVANPANTNAL 369
            +ALE++A  + KV+VV NPANTN L
Sbjct:  114 TALEKYAKKSVKVIVVGNPANTNCL 138

The following BLAST results are available for this feature:

BLAST of CN184794 vs. ExPASy Swiss-Prot

BLAST of CN184794 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 185


Match Name	E-value	Identity	Description
MDHC_MAIZE	5.946e-55	90.22	Malate dehydrogenase, cytoplasmic OS=Zea mays PE=2...	[more]
MDHC_MEDSA	6.466e-54	89.13	Malate dehydrogenase, cytoplasmic OS=Medicago sati...	[more]
MDHC_MESCR	6.466e-54	88.04	Malate dehydrogenase, cytoplasmic OS=Mesembryanthe...	[more]
MDHC2_ARATH	4.137e-53	85.87	Malate dehydrogenase, cytoplasmic 2 OS=Arabidopsis...	[more]
MDHC1_ARATH	9.163e-53	86.96	Malate dehydrogenase, cytoplasmic 1 OS=Arabidopsis...	[more]
MDHC_ORYSJ	1.194e-52	86.96	Malate dehydrogenase, cytoplasmic OS=Oryza sativa ...	[more]
MDHC_BETVU	1.557e-52	88.04	Malate dehydrogenase, cytoplasmic OS=Beta vulgaris...	[more]
MDHC_PIG	1.344e-34	72.53	Malate dehydrogenase, cytoplasmic OS=Sus scrofa GN...	[more]
MDHC_RAT	2.281e-34	70.33	Malate dehydrogenase, cytoplasmic OS=Rattus norveg...	[more]
MDHC_MOUSE	2.281e-34	70.33	Malate dehydrogenase, cytoplasmic OS=Mus musculus ...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS04_0006B22_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006B22, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN184794 ID=CN184794; Name=CN184794; organism=Citrus sinensis; type=EST; length=381bp
TTTGATGATTGCGCACCACGTTGTTGGACTGTGGTTATGAATTCTCCATT
CAACCATGCATCATCCTTGACAAGCTCACGGACAGGCTTTTCCCCAGCTG
CAGTATTGACAGTTGCATGGTTGACATCAGGGTACTGAGATGATGAATGA
TTTCCCCAAATAATCACATTCTTAACATCAGATACTTGGACATTCAGTTT
CTCAGATATTTGTCCCAATGCCCTGTTATGATCCAATCTGGTCAAGCATG
TAATGTTCTTAGCAGGGATTGATGGCGCAAATTTCTTTCAGAATCAATGC
ATTGGTGTTTGCAGGGTTAGCGACAACTAGAACCTTGCAGTTCGGAGCTG
CGTGCTGCTCCAGGGCAGAAAGCCTGAGCCT