CN184778 | Citrus Genome Database

Overview


Name	CN184778
Unique Name	CN184778
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	729

Libraries


Library Name	Type
Ruby Orange Developing Flower cDNA Library	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 136.732 bits (343), Expect = 1.402e-54
Identity = 61/105 (58.10%), Postives = 74/105 (70.48%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQ-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K ++  FFAQT HETTGGW  AP G YAWGYC++R+                 PG+ Y+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFKSA+WFW
Sbjct:  127 KREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFW 231

HSP 2 Score: 91.2781 bits (225), Expect = 1.402e-54
Identity = 47/88 (53.41%), Postives = 59/88 (67.05%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTD--GGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 367
            G +   CG G  C  +  G  T   GG+LG IIS  MF+ +L +RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFF
Sbjct:   48 GNTNDYCGPGN-CQSQCPGGPTPPGGGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFF 134

HSP 3 Score: 25.7942 bits (55), Expect = 1.402e-54
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MT Q PKPSCH+
Sbjct:  232 MTPQSPKPSCHD 243

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 134.42 bits (337), Expect = 1.824e-54
Identity = 59/105 (56.19%), Postives = 75/105 (71.43%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQ-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K+++  FF QT HETTGGW  AP G Y+WGYC+ ++ +PAS             G+ YYGRGP+QL+WN NY  CG A+GVDLLNNPDL+A D V++FK+AIWFW
Sbjct:  138 KKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLLNNPDLVANDAVIAFKAAIWFW 242

HSP 2 Score: 88.9669 bits (219), Expect = 1.824e-54
Identity = 39/62 (62.90%), Postives = 50/62 (80.65%), Query Frame = 2

Query:  182 GELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 367
            G+L  IIS   F+D+L +RNDA CPARGFYTY+AFI AA++FPGFG +G+   RK+E+AAFF
Sbjct:   84 GDLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFF 145

HSP 3 Score: 30.0314 bits (66), Expect = 1.824e-54
Identity = 11/11 (100.00%), Postives = 11/11 (100.00%), Query Frame = 1

Query:  658 MTAQPPKPSCH 690
            MTAQPPKPSCH
Sbjct:  243 MTAQPPKPSCH 253

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 136.732 bits (343), Expect = 5.233e-54
Identity = 61/105 (58.10%), Postives = 74/105 (70.48%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQ-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K ++  FFAQT HETTGGW  AP G YAWGYC++R+                 PG+ Y+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFKSA+WFW
Sbjct:  132 KREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSALWFW 236

HSP 2 Score: 89.3521 bits (220), Expect = 5.233e-54
Identity = 40/63 (63.49%), Postives = 50/63 (79.37%), Query Frame = 2

Query:  179 GGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 367
            GG+LG IIS  MF+ +L +RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFF
Sbjct:   77 GGDLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFF 139

HSP 3 Score: 25.7942 bits (55), Expect = 5.233e-54
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MT Q PKPSCH+
Sbjct:  237 MTPQSPKPSCHD 248

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHIT_PHAVU (Endochitinase OS=Phaseolus vulgaris PE=1 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 6.810e-54
Identity = 60/111 (54.05%), Postives = 76/111 (68.47%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWNDCTATK 677
            K ++  F  QT HETTGGW  AP G YAWGYC++R+ +P++              QYYGRGPIQ++WN NY +CG A+GVDLLN PDL+ATD V+SFKSA+WFW    + K
Sbjct:  128 KREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERNPSTYCSATPQFPCAPGQQYYGRGPIQISWNYNYGQCGRAIGVDLLNKPDLVATDSVISFKSALWFWMTAQSPK 238

HSP 2 Score: 88.9669 bits (219), Expect = 6.810e-54
Identity = 43/85 (50.59%), Postives = 55/85 (64.71%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTDGGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 364
            G +   CG G  C  +  G      +L  +ISR  F+ +L +RND  CPA+GFYTYDAFI AA+A+P FGN+G+   RKREIAAF
Sbjct:   52 GSTTDYCGPG--CQSQCGGPSPAPTDLSALISRSTFDQMLKHRNDGACPAKGFYTYDAFIAAAKAYPSFGNTGDTATRKREIAAF 134

HSP 3 Score: 23.8682 bits (50), Expect = 6.810e-54
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MTAQ PKPS H+
Sbjct:  232 MTAQSPKPSSHD 243

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 134.806 bits (338), Expect = 6.812e-54
Identity = 57/105 (54.29%), Postives = 77/105 (73.33%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQ-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K+++  FF QT HETTGGW  AP G Y+WGYC+ ++ +P+S+            G+ YYGRGP+QL+WN NY +CG A+G DLLNNPDL++ DPV++FK+AIWFW
Sbjct:  123 KKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCASGKSYYGRGPMQLSWNYNYGQCGRAIGSDLLNNPDLVSNDPVIAFKAAIWFW 227

HSP 2 Score: 91.6633 bits (226), Expect = 6.812e-54
Identity = 41/62 (66.13%), Postives = 50/62 (80.65%), Query Frame = 2

Query:  182 GELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 367
            G+L  IISR  F+D+L +RND  CPARGFYTYDAFI AA++FPGFG +G+   RK+EIAAFF
Sbjct:   69 GDLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKEIAAFF 130

HSP 3 Score: 25.0238 bits (53), Expect = 6.812e-54
Identity = 9/11 (81.82%), Postives = 9/11 (81.82%), Query Frame = 1

Query:  658 MTAQPPKPSCH 690
            MT Q PKPSCH
Sbjct:  228 MTPQSPKPSCH 238

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 130.568 bits (327), Expect = 6.816e-54
Identity = 59/105 (56.19%), Postives = 74/105 (70.48%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIR-QVSPASSDXXXXXXXXXLPGQYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K ++  F AQT HETTGGW  AP G YAWGYCY+R Q SP +              +YYGRGPIQ+++N NY + G+A+GVDL+NNPDL+ATD V+SFK+A WFW
Sbjct:  117 KREIAAFLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGPIQISYNYNYGQAGKAIGVDLVNNPDLVATDAVISFKTAFWFW 221

HSP 2 Score: 95.9005 bits (237), Expect = 6.816e-54
Identity = 46/85 (54.12%), Postives = 59/85 (69.41%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTDGGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 364
            G +A  CGSG       TG      ++G++I+R MF D+L +RN+  CP +GFYTYDAFI AA+AFPGFG +G+ T RKREIAAF
Sbjct:   45 GNTADYCGSGCQSQCSSTG------DIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKREIAAF 123

HSP 3 Score: 25.0238 bits (53), Expect = 6.816e-54
Identity = 9/11 (81.82%), Postives = 9/11 (81.82%), Query Frame = 1

Query:  658 MTAQPPKPSCH 690
            MT Q PKPSCH
Sbjct:  222 MTPQSPKPSCH 232

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHI5_PHAVU (Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 138.658 bits (348), Expect = 4.305e-53
Identity = 60/111 (54.05%), Postives = 76/111 (68.47%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFWNDCTATK 677
            K ++  F  QT HETTGGW  AP G YAWGYC++R+ +P++              QYYGRGPIQ++WN NY +CG A+GVDLLN PDL+ATD V+SFKSA+WFW    + K
Sbjct:  127 KREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERNPSAYCSATPQFPCAPGQQYYGRGPIQISWNYNYGQCGRAIGVDLLNKPDLVATDSVISFKSALWFWMTAQSPK 237

HSP 2 Score: 86.2705 bits (212), Expect = 4.305e-53
Identity = 42/85 (49.41%), Postives = 54/85 (63.53%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTDGGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 364
            G +   CG    C  +  G      +L  +ISR  F+ +L +RND  CPA+GFYTYDAFI AA+A+P FGN+G+   RKREIAAF
Sbjct:   51 GSTTDYCGKD--CQSQCGGPSPAPTDLSALISRSTFDQVLKHRNDGACPAKGFYTYDAFIAAAKAYPSFGNTGDTATRKREIAAF 133

HSP 3 Score: 23.8682 bits (50), Expect = 4.305e-53
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MTAQ PKPS H+
Sbjct:  231 MTAQSPKPSSHD 242

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHI2_PEA (Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=2)

HSP 1 Score: 142.51 bits (358), Expect = 4.306e-53
Identity = 60/104 (57.69%), Postives = 76/104 (73.08%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQYYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K ++  F  QT HETTGGW  AP G YAWGYC++R+ +P++              QYYGRGPIQ++WN NY +CG A+GVDLLNNPDL+ATDPV+SFK+A+WFW
Sbjct:  120 KREIAAFLGQTSHETTGGWPTAPDGPYAWGYCFLREQNPSTYCQASSEFPCASGKQYYGRGPIQISWNYNYGQCGRAIGVDLLNNPDLVATDPVISFKTALWFW 223

HSP 2 Score: 80.4925 bits (197), Expect = 4.306e-53
Identity = 39/85 (45.88%), Postives = 55/85 (64.71%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTDGGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 364
            G +++ CG G  C  + +G  + GG L  +IS + F ++L +RND  C  + FYTYDAF+ AA+AFP F N G+   +KREIAAF
Sbjct:   45 GSTSEYCGDG--CQSQCSG-SSGGGTLSSLISGDTFNNMLKHRNDNACQGKPFYTYDAFLSAAKAFPNFANKGDTATKKREIAAF 126

HSP 3 Score: 25.7942 bits (55), Expect = 4.306e-53
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MT Q PKPSCH+
Sbjct:  224 MTPQSPKPSCHD 235

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 135.576 bits (340), Expect = 1.606e-52
Identity = 60/105 (57.14%), Postives = 74/105 (70.48%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQ-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K ++  FFAQT HETTGGW  AP G YAWGYC++R+                 PG+ Y+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFK+A+WFW
Sbjct:  131 KREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPGDYCPPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKTALWFW 235

HSP 2 Score: 85.5001 bits (210), Expect = 1.606e-52
Identity = 44/89 (49.44%), Postives = 57/89 (64.04%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTD---GGELGKIISREMFEDLLPYRNDARCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 367
            G +   CGSG  C  +  G G     GG+LG  IS  MF+ +L +RN+  C  + FY+Y+AFI AA++FPGFG SG+   RKREIAAFF
Sbjct:   51 GNTNDYCGSGN-CQSQCPGGGPGPGPGGDLGSAISNSMFDQMLKHRNENSCQGKNFYSYNAFINAARSFPGFGTSGDINARKREIAAFF 138

HSP 3 Score: 25.7942 bits (55), Expect = 1.606e-52
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MT Q PKPSCH+
Sbjct:  236 MTPQSPKPSCHD 247

[Close]

BLAST of CN184778 vs. ExPASy Swiss-Prot
Match: CHI4_SOLTU (Endochitinase 4 (Fragment) OS=Solanum tuberosum GN=CHTB4 PE=2 SV=1)

HSP 1 Score: 137.502 bits (345), Expect = 3.544e-52
Identity = 62/105 (59.05%), Postives = 74/105 (70.48%), Query Frame = 3

Query:  345 KEKLLPFFAQTGHETTGGWTDAPGGEYAWGYCYIRQVSPASSDXXXXXXXXXLPGQ-YYGRGPIQLTWNCNYLRCGEALGVDLLNNPDLLATDPVLSFKSAIWFW 656
            K ++  FFAQT HETTGGW  AP G YAWGYC++R+                 PG+ Y+GRGPIQ++ N NY  CG A+GVDLLNNPDL+ATDPV+SFKSAIWFW
Sbjct:  131 KREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLLNNPDLVATDPVISFKSAIWFW 235

HSP 2 Score: 82.4185 bits (202), Expect = 3.544e-52
Identity = 41/87 (47.13%), Postives = 54/87 (62.07%), Query Frame = 2

Query:  110 GGSAQNCGSGVVCGERDTGHGTDGGELGKIISREMFEDLLPYRNDARCPARG-FYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 367
            G     C SG        G G  GG++G +IS  MF+ +L +RND  C  +  FY+Y+AFI AA++F GFG +G+ T RKREIAAFF
Sbjct:   52 GKCQSQCPSGPSPKPPTPGPGPSGGDIGSVISNSMFDQMLKHRNDNACQGKNNFYSYNAFINAARSFGGFGTTGDTTARKREIAAFF 138

HSP 3 Score: 25.7942 bits (55), Expect = 3.544e-52
Identity = 9/12 (75.00%), Postives = 10/12 (83.33%), Query Frame = 1

Query:  658 MTAQPPKPSCHE 693
            MT Q PKPSCH+
Sbjct:  236 MTPQSPKPSCHD 247

The following BLAST results are available for this feature:

BLAST of CN184778 vs. ExPASy Swiss-Prot

BLAST of CN184778 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55


Match Name	E-value	Identity	Description
CHI2_TOBAC	1.402e-54	58.10	Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1...	[more]
CHIB_ARATH	1.824e-54	56.19	Basic endochitinase B OS=Arabidopsis thaliana GN=C...	[more]
CHI1_TOBAC	5.233e-54	58.10	Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1...	[more]
CHIT_PHAVU	6.810e-54	54.05	Endochitinase OS=Phaseolus vulgaris PE=1 SV=1	[more]
CHI2_BRANA	6.812e-54	54.29	Endochitinase CH25 OS=Brassica napus PE=2 SV=1	[more]
CHIB_VITVI	6.816e-54	56.19	Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE...	[more]
CHI5_PHAVU	4.305e-53	54.05	Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1	[more]
CHI2_PEA	4.306e-53	57.69	Endochitinase A2 OS=Pisum sativum GN=CHI2 PE=1 SV=...	[more]
CHIT_SOLTU	1.606e-52	57.14	Endochitinase OS=Solanum tuberosum PE=2 SV=1	[more]
CHI4_SOLTU	3.544e-52	59.05	Endochitinase 4 (Fragment) OS=Solanum tuberosum GN...	[more]

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Properties


Property Name	Value
Genbank description	UCRCS04_0006B10_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0006B10, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CN184778 ID=CN184778; Name=CN184778; organism=Citrus sinensis; type=EST; length=729bp
CCCCCCCGGGCGGCTTGCAGGGATATTCGCGCACAGAAGGCTCTTGCTTT
CAAAATGAGGCTCATNTGGGTCCTTACTAATTTTCTCTCTAGTTTTATCT
TTTGTACTAGGAGGTTCAGCACAGAATTGTGGAAGCGGCGTTGTGTGCGG
TGAACGTGATACTGGCCACGGGACGGATGGCGGTGAACTGGGCAAGATCA
TCTCAAGGGAGATGTTTGAAGACTTGCTTCCGTATAGAAATGATGCCCGA
TGCCCTGCCAGAGGCTTCTACACTTATGATGCTTTTATAGAGGCAGCCCA
AGCTTTTCCAGGCTTTGGTAACTCTGGAAATGAAACCATGCGTAAAAGAG
AAATTGCTGCCTTTTTTTGCCCAAACTGGCCATGAAACTACTGGGGGATG
GACCGATGCACCTGGTGGAGAATATGCTTGGGGATATTGCTATATTAGGC
AAGTTAGCCCTGCGTCGTCCGACTACTGTTATCCTTACTGTCCATGTCCC
TTGCCAGGGCAATACTATGGCCGAGGTCCAATTCAACTCACTTGGAATTG
CAATTACTTAAGGTGCGGAGAAGCCTTGGGGGTGGACTTGTTGAACAATC
CAGACCTACTTGCCACTGATCCAGTACTATCATTCAAGTCAGCAATCTGG
TTCTGGAATGACTGCACAGCCACCAAAGCCATCGTGCCACGAAAGTCATT
ATCGACGAATGGAAACCATCAGCAAATGA