EY666420 | Citrus Genome Database

Overview


Name	EY666420
Unique Name	EY666420
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	845

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CSLB4_ARATH (Cellulose synthase-like protein B4 OS=Arabidopsis thaliana GN=CSLB4 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 7.807e-33
Identity = 36/88 (40.91%), Postives = 47/88 (53.41%), Query Frame = 2

Query:   56 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            MA + S   PL E++ HK+ + RA                          W +AFLCE+CFTF+W+LI N KW+P  YKTYP+RL ER
Sbjct:    1 MAESSSPLPPLCERISHKSYFLRAVDLTILGLLLSLLLYRILHVNQKDTVWIVAFLCETCFTFVWLLITNIKWSPADYKTYPERLDER 88

HSP 2 Score: 63.929 bits (154), Expect = 7.807e-33
Identity = 29/40 (72.50%), Postives = 34/40 (85.00%), Query Frame = 1

Query:  430 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPVH 549
            R+ ELPPVDMFVTTAD   EPP+I VNTVLSLLA++YP +
Sbjct:   88 RVHELPPVDMFVTTADPVREPPLIVVNTVLSLLAVNYPAN 127

HSP 3 Score: 39.6614 bits (91), Expect = 7.807e-33
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            SL E SKFAK+WVPFCKKY
Sbjct:  145 SLKEASKFAKIWVPFCKKY 163

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CSLB2_ARATH (Cellulose synthase-like protein B2 OS=Arabidopsis thaliana GN=CSLB2 PE=2 SV=1)

HSP 1 Score: 69.3218 bits (168), Expect = 1.600e-30
Identity = 33/88 (37.50%), Postives = 44/88 (50.00%), Query Frame = 2

Query:   56 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            MA++  S  PLYE + +K+   RA                      +G  W +AFLCESCF+F+W+L    KW+P   K YP RL ER
Sbjct:    1 MADSSCSLPPLYENISYKSYILRAVDLTILGLLFSLLLHRILYMSQNGIIWLVAFLCESCFSFVWLLSTCTKWSPAETKPYPDRLDER 88

HSP 2 Score: 56.6102 bits (135), Expect = 1.600e-30
Identity = 26/40 (65.00%), Postives = 32/40 (80.00%), Query Frame = 1

Query:  430 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPVH 549
            R+ +LP VDMFV TAD   EPPI+ VNTVLSLLA++YP +
Sbjct:   88 RVYDLPSVDMFVPTADPVREPPIMVVNTVLSLLAVNYPAN 127

HSP 3 Score: 39.6614 bits (91), Expect = 1.600e-30
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            SL E SKFAK+WVPFCKKY
Sbjct:  145 SLKEASKFAKIWVPFCKKY 163

HSP 4 Score: 28.8758 bits (63), Expect = 1.600e-30
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD CSPL +
Sbjct:  131 CYVSDDGCSPLTY 143

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CSLB6_ARATH (Cellulose synthase-like protein B6 OS=Arabidopsis thaliana GN=CSLB6 PE=2 SV=2)

HSP 1 Score: 67.781 bits (164), Expect = 1.268e-29
Identity = 33/88 (37.50%), Postives = 45/88 (51.14%), Query Frame = 2

Query:   56 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            MA++ SS LPL E++ HK+   R                       +   W +AFLCESCF+F+W++I   KW+P   K YP RL ER
Sbjct:    1 MADSSSSLLPLCERISHKSYILRIVDLTILVLLFSLLWYRILHMCENNTIWLVAFLCESCFSFMWLIITCIKWSPAEDKPYPNRLDER 88

HSP 2 Score: 58.151 bits (139), Expect = 1.268e-29
Identity = 27/40 (67.50%), Postives = 32/40 (80.00%), Query Frame = 1

Query:  430 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPVH 549
            R+ +LP VDMFV TAD   EPPII VNTVLSLLA++YP +
Sbjct:   88 RVHDLPSVDMFVPTADPVREPPIIVVNTVLSLLAVNYPAN 127

HSP 3 Score: 36.5798 bits (83), Expect = 1.268e-29
Identity = 14/19 (73.68%), Postives = 15/19 (78.95%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            SL E SKF K+W PFCKKY
Sbjct:  145 SLKEASKFVKIWAPFCKKY 163

HSP 4 Score: 28.8758 bits (63), Expect = 1.268e-29
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD CSPL +
Sbjct:  131 CYVSDDGCSPLTY 143

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CSLB3_ARATH (Cellulose synthase-like protein B3 OS=Arabidopsis thaliana GN=CSLB3 PE=2 SV=1)

HSP 1 Score: 74.3294 bits (181), Expect = 1.984e-29
Identity = 35/88 (39.77%), Postives = 49/88 (55.68%), Query Frame = 2

Query:   56 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            MA++ SS  PL EK+ +KN + R                       +   W +AFLCES F+FIW+LI + KW+P +YK+YP+RL ER
Sbjct:    1 MADSSSSLPPLCEKISYKNYFLRVVDLTILGFLFSLLLYRILLMNQNNSVWVVAFLCESFFSFIWLLITSIKWSPASYKSYPERLDER 88

HSP 2 Score: 56.9954 bits (136), Expect = 1.984e-29
Identity = 25/40 (62.50%), Postives = 32/40 (80.00%), Query Frame = 1

Query:  430 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPVH 549
            R+ +LP VDMFVTTAD   EPPI+  NT+LSLLA++YP +
Sbjct:   88 RVHDLPSVDMFVTTADPVREPPILVANTLLSLLAVNYPAN 127

HSP 3 Score: 39.6614 bits (91), Expect = 1.984e-29
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            SL E SKFAK+WVPFCKKY
Sbjct:  145 SLKEASKFAKIWVPFCKKY 163

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CSLB1_ARATH (Cellulose synthase-like protein B1 OS=Arabidopsis thaliana GN=CSLB1 PE=2 SV=2)

HSP 1 Score: 55.0694 bits (131), Expect = 6.361e-26
Identity = 28/88 (31.82%), Postives = 40/88 (45.45%), Query Frame = 2

Query:   56 MANTKSSCLPLYEKVFHKNTYHRAXXXXXXXXXXXXXXXXXXXXKNHGFAWFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            MA++  S  PL E++ + N + RA                      +   W +AF CESCF  + +LI   KW+P   K +P RL ER
Sbjct:    1 MADSSFSLPPLCERISYTNYFLRAVYLTVLGLFFSLLLHRIRHTSEYDNVWLVAFFCESCFFLVCLLITCLKWSPADTKPFPDRLDER 88

HSP 2 Score: 55.0694 bits (131), Expect = 6.361e-26
Identity = 25/40 (62.50%), Postives = 32/40 (80.00%), Query Frame = 1

Query:  430 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPVH 549
            R+ +LP VDMFV TAD   EPPI+ V+TVLSLLA++YP +
Sbjct:   88 RVHDLPSVDMFVPTADPVREPPIMVVDTVLSLLAVNYPAN 127

HSP 3 Score: 39.6614 bits (91), Expect = 6.361e-26
Identity = 16/19 (84.21%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            SL E SKFAK+WVPFCKKY
Sbjct:  145 SLKEASKFAKIWVPFCKKY 163

HSP 4 Score: 28.8758 bits (63), Expect = 6.361e-26
Identity = 10/13 (76.92%), Postives = 11/13 (84.62%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD CSPL +
Sbjct:  131 CYVSDDGCSPLTY 143

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CSLH1_ORYSJ (Cellulose synthase-like protein H1 OS=Oryza sativa subsp. japonica GN=CSLH1 PE=2 SV=2)

HSP 1 Score: 66.2402 bits (160), Expect = 6.555e-24
Identity = 31/38 (81.58%), Postives = 34/38 (89.47%), Query Frame = 1

Query:  430 RIQELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYP 543
            RI ELP VDMFVTTAD  LEPP++TVNTVLSLLA+DYP
Sbjct:   87 RIDELPAVDMFVTTADPVLEPPLVTVNTVLSLLALDYP 124

HSP 2 Score: 48.521 bits (114), Expect = 6.555e-24
Identity = 18/35 (51.43%), Postives = 26/35 (74.29%), Query Frame = 2

Query:  215 AFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            A  CE+ FTF+W+L VN KW+P+ + T+P+ L ER
Sbjct:   53 ALACEAWFTFMWLLNVNAKWSPVRFDTFPENLAER 87

HSP 3 Score: 29.261 bits (64), Expect = 6.555e-24
Identity = 9/19 (47.37%), Postives = 16/19 (84.21%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            +L E ++FA+ WVPFC+++
Sbjct:  146 ALREAARFARTWVPFCRRH 164

HSP 4 Score: 27.7202 bits (60), Expect = 6.555e-24
Identity = 10/11 (90.91%), Postives = 10/11 (90.91%), Query Frame = 2

Query:  560 CYVSDDACSPL 592
            CYVSDD CSPL
Sbjct:  132 CYVSDDGCSPL 142

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CESA2_ORYSJ (Probable cellulose synthase A catalytic subunit 2 [UDP-forming] OS=Oryza sativa subsp. japonica GN=CESA2 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.095e-17
Identity = 23/36 (63.89%), Postives = 30/36 (83.33%), Query Frame = 1

Query:  439 ELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPV 546
            +L PVD+FV+T D   EPP++T NTVLS+LA+DYPV
Sbjct:  344 QLAPVDIFVSTVDPMKEPPLVTANTVLSILAVDYPV 379

HSP 2 Score: 37.3502 bits (85), Expect = 1.095e-17
Identity = 14/20 (70.00%), Postives = 18/20 (90.00%), Query Frame = 3

Query:  600 NSLVETSKFAKLWVPFCKKY 659
            ++L ETS+FA+ WVPFCKKY
Sbjct:  397 DALAETSEFARKWVPFCKKY 416

HSP 3 Score: 36.5798 bits (83), Expect = 1.095e-17
Identity = 16/38 (42.11%), Postives = 21/38 (55.26%), Query Frame = 2

Query:  206 WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            W L+ +CE  F   WIL    KW+P+  +TY  RL  R
Sbjct:  298 WLLSVICEIWFALSWILDQFPKWSPINRETYLDRLALR 335

HSP 4 Score: 21.9422 bits (45), Expect = 1.095e-17
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD  + L F
Sbjct:  384 CYVSDDGAAMLTF 396

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CESA2_ORYSI (Probable cellulose synthase A catalytic subunit 2 [UDP-forming] OS=Oryza sativa subsp. indica GN=CESA2 PE=2 SV=1)

HSP 1 Score: 54.299 bits (129), Expect = 1.095e-17
Identity = 23/36 (63.89%), Postives = 30/36 (83.33%), Query Frame = 1

Query:  439 ELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPV 546
            +L PVD+FV+T D   EPP++T NTVLS+LA+DYPV
Sbjct:  344 QLAPVDIFVSTVDPMKEPPLVTANTVLSILAVDYPV 379

HSP 2 Score: 37.3502 bits (85), Expect = 1.095e-17
Identity = 14/20 (70.00%), Postives = 18/20 (90.00%), Query Frame = 3

Query:  600 NSLVETSKFAKLWVPFCKKY 659
            ++L ETS+FA+ WVPFCKKY
Sbjct:  397 DALAETSEFARKWVPFCKKY 416

HSP 3 Score: 36.5798 bits (83), Expect = 1.095e-17
Identity = 16/38 (42.11%), Postives = 21/38 (55.26%), Query Frame = 2

Query:  206 WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            W L+ +CE  F   WIL    KW+P+  +TY  RL  R
Sbjct:  298 WLLSVICEIWFALSWILDQFPKWSPINRETYLDRLALR 335

HSP 4 Score: 21.9422 bits (45), Expect = 1.095e-17
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD  + L F
Sbjct:  384 CYVSDDGAAMLTF 396

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CESA4_ARATH (Cellulose synthase A catalytic subunit 4 [UDP-forming] OS=Arabidopsis thaliana GN=CESA4 PE=1 SV=1)

HSP 1 Score: 54.6842 bits (130), Expect = 1.415e-17
Identity = 24/37 (64.86%), Postives = 31/37 (83.78%), Query Frame = 1

Query:  439 ELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPVH 549
            +L PVD+FV+T D   EPPIIT NT+LS+LA+DYPV+
Sbjct:  290 KLAPVDVFVSTVDPLKEPPIITANTILSILAVDYPVN 326

HSP 2 Score: 37.7354 bits (86), Expect = 1.415e-17
Identity = 15/26 (57.69%), Postives = 21/26 (80.77%), Query Frame = 3

Query:  582 AHLSISNSLVETSKFAKLWVPFCKKY 659
            A + + ++L ETS+FA+ WVPFCKKY
Sbjct:  337 ASMLLFDTLSETSEFARRWVPFCKKY 362

HSP 3 Score: 35.4242 bits (80), Expect = 1.415e-17
Identity = 15/38 (39.47%), Postives = 20/38 (52.63%), Query Frame = 2

Query:  206 WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            W ++ +CE  F   WIL    KW P+  +TY  RL  R
Sbjct:  244 WLISVICEIWFALSWILDQFPKWFPINRETYLDRLSMR 281

HSP 4 Score: 21.9422 bits (45), Expect = 1.415e-17
Identity = 9/13 (69.23%), Postives = 9/13 (69.23%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD  S L F
Sbjct:  330 CYVSDDGASMLLF 342

[Close]

BLAST of EY666420 vs. ExPASy Swiss-Prot
Match: CESA3_ORYSJ (Probable cellulose synthase A catalytic subunit 3 [UDP-forming] OS=Oryza sativa subsp. japonica GN=CESA3 PE=2 SV=1)

HSP 1 Score: 53.1434 bits (126), Expect = 1.083e-16
Identity = 22/36 (61.11%), Postives = 29/36 (80.56%), Query Frame = 1

Query:  439 ELPPVDMFVTTADAELEPPIITVNTVLSLLAIDYPV 546
            +L P+D FV+T D   EPP++T NTVLS+LA+DYPV
Sbjct:  354 QLAPIDFFVSTVDPLKEPPLVTANTVLSILAVDYPV 389

HSP 2 Score: 37.3502 bits (85), Expect = 1.083e-16
Identity = 15/19 (78.95%), Postives = 17/19 (89.47%), Query Frame = 3

Query:  603 SLVETSKFAKLWVPFCKKY 659
            +L ETS+FAK WVPFCKKY
Sbjct:  408 ALSETSEFAKKWVPFCKKY 426

HSP 3 Score: 34.2686 bits (77), Expect = 1.083e-16
Identity = 15/38 (39.47%), Postives = 20/38 (52.63%), Query Frame = 2

Query:  206 WFLAFLCESCFTFIWILIVNCKWTPLTYKTYPQRLQER 319
            W ++ +CE  F   WIL    KW P+  +TY  RL  R
Sbjct:  308 WLISVICEIWFAMSWILDQFPKWFPIERETYLDRLTLR 345

HSP 4 Score: 21.9422 bits (45), Expect = 1.083e-16
Identity = 8/13 (61.54%), Postives = 9/13 (69.23%), Query Frame = 2

Query:  560 CYVSDDACSPLNF 598
            CYVSDD  + L F
Sbjct:  394 CYVSDDGAAMLTF 406

The following BLAST results are available for this feature:

BLAST of EY666420 vs. ExPASy Swiss-Prot

BLAST of EY666420 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 42


Match Name	E-value	Identity	Description
CSLB4_ARATH	7.807e-33	40.91	Cellulose synthase-like protein B4 OS=Arabidopsis ...	[more]
CSLB2_ARATH	1.600e-30	37.50	Cellulose synthase-like protein B2 OS=Arabidopsis ...	[more]
CSLB6_ARATH	1.268e-29	37.50	Cellulose synthase-like protein B6 OS=Arabidopsis ...	[more]
CSLB3_ARATH	1.984e-29	39.77	Cellulose synthase-like protein B3 OS=Arabidopsis ...	[more]
CSLB1_ARATH	6.361e-26	31.82	Cellulose synthase-like protein B1 OS=Arabidopsis ...	[more]
CSLH1_ORYSJ	6.555e-24	81.58	Cellulose synthase-like protein H1 OS=Oryza sativa...	[more]
CESA2_ORYSJ	1.095e-17	63.89	Probable cellulose synthase A catalytic subunit 2 ...	[more]
CESA2_ORYSI	1.095e-17	63.89	Probable cellulose synthase A catalytic subunit 2 ...	[more]
CESA4_ARATH	1.415e-17	64.86	Cellulose synthase A catalytic subunit 4 [UDP-form...	[more]
CESA3_ORYSJ	1.083e-16	61.11	Probable cellulose synthase A catalytic subunit 3 ...	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-017-F09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY666420 ID=EY666420; Name=EY666420; organism=Citrus sinensis; type=EST; length=845bp
CCCACGCGTCCGAAACACCCCATATAAAAAAAACGAGTACTTGTTTTTCA
AGCTCATGGCCAATACAAAGTCGTCATGTCTCCCTCTATACGAAAAAGTT
TTTCATAAAAACACATATCATAGGGCTTTAGATGTTATCATCTTGTTCCT
TCTTCTATGTCTGCTCTTCTATCGGCTCTCAACGTTGAAGAATCATGGAT
TTGCTTGGTTTCTTGCTTTTCTATGTGAGTCTTGTTTTACTTTCATCTGG
ATTCTCATCGTCAACTGCAAGTGGACTCCTTTGACCTACAAAACATATCC
CCAACGTCTCCAAGAAAGGTAACAAATCAAATAACGTTAGCATTAATAAT
TTCCGCATCATTTGCAATTGTCTTTGTCATTTTGCAAGTTACTACACACA
CACACACACACACATGTGTACGACTCAACAGGATACAGGAGCTGCCCCCA
GTGGACATGTTTGTGACAACTGCTGATGCTGAACTCGAGCCGCCAATCAT
CACTGTAAACACAGTGCTGTCTTTGTTGGCAATTGATTATCCAGTTCACA
AGCTCGCCTTGCTATGTTTCTGATGATGCCTGCTCACCTCTCAATTTCTA
ACTCTCTTGTTGAAACTTCTAAATTCGCTAAACTTTGGGTTCCATTCTGT
AAAAAGTATAAAGGTTCAAATTAGAACTCCCTTTAAAATACTTCCCAAAA
AGTGAGCCCTCCAATTCCAGTTAAGGGGGAAATTCCTGCAAAAATGGGAA
AAAACTGAAAGGAAGGAATGAACGGTTTTCTCCCAAACAAAAAACGAGTG
AATCACATCCACACTAACCGAAGCTTTTCTCGGCGCAACCCAAGC