CN184477

Overview
NameCN184477
Unique NameCN184477
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length366
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPPS_CATRO (Geranylgeranyl pyrophosphate synthetase, chloroplastic OS=Catharanthus roseus GN=GGPS1 PE=3 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 4.318e-24
Identity = 47/93 (50.54%), Postives = 56/93 (60.22%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSL+HDDLP +DNDDLRRGKPTNHKVFGE              FEH+A  T GVS++R+VR + E             QV D+ SEG
Sbjct:  134 VEMIHTMSLMHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLAFAFEHIATATKGVSSERIVRVVGELAKCIGSEGLVAGQVVDVCSEG 226

HSP 2 Score: 36.965 bits (84), Expect = 4.318e-24
Identity = 18/28 (64.29%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  282 DVSLSDLEYILVHKTAKLLEASVVCGVI 365
            DV L  LE+I +HKTA LLE SVV G I
Sbjct:  229 DVGLEHLEFIHIHKTAALLEGSVVLGAI 256
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPPS_HEVBR (Geranylgeranyl pyrophosphate synthetase, chloroplastic OS=Hevea brasiliensis GN=GGPS PE=1 SV=1)

HSP 1 Score: 93.2041 bits (230), Expect = 1.237e-23
Identity = 47/93 (50.54%), Postives = 56/93 (60.22%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSLIHDDLP +DNDDLRRGKPTNH VFGE              FEH+A  T+ VS+ R+VRA+ E             QV D++SEG
Sbjct:  147 VEMIHTMSLIHDDLPCMDNDDLRRGKPTNHIVFGEDVAVLAGDALLAFAFEHIAVSTLNVSSARIVRAVGELAKAIGAEGLVAGQVVDINSEG 239

HSP 2 Score: 36.5798 bits (83), Expect = 1.237e-23
Identity = 17/28 (60.71%), Postives = 21/28 (75.00%), Query Frame = 3 
Query:  282 DVSLSDLEYILVHKTAKLLEASVVCGVI 365
            +V L  LE+I +HKTAKLLE +VV G I
Sbjct:  242 EVDLEKLEFIHIHKTAKLLEGAVVLGAI 269
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPP1_ARATH (Geranylgeranyl pyrophosphate synthetase 1, chloroplastic OS=Arabidopsis thaliana GN=GGPS1 PE=2 SV=2)

HSP 1 Score: 88.1965 bits (217), Expect = 4.932e-22
Identity = 49/95 (51.58%), Postives = 54/95 (56.84%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVG--VSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSLIHDDLP +DNDDLRRGKPTNHKVFGE              FEH+A  T    VS  RVVRA+ E             QV D+ SEG
Sbjct:  144 VEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVFGEDVAVLAGDALLSFAFEHLASATSSDVVSPVRVVRAVGELAKAIGTEGLVAGQVVDISSEG 238

HSP 2 Score: 36.1946 bits (82), Expect = 4.932e-22
Identity = 18/28 (64.29%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  282 DVSLSDLEYILVHKTAKLLEASVVCGVI 365
            DV L  LE+I +HKTA LLEAS V G I
Sbjct:  243 DVGLEHLEFIHLHKTAALLEASAVLGAI 270
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPPS_CAPAN (Geranylgeranyl pyrophosphate synthetase, chloroplastic OS=Capsicum annuum GN=GGPS1 PE=3 SV=1)

HSP 1 Score: 87.0409 bits (214), Expect = 6.417e-22
Identity = 42/93 (45.16%), Postives = 51/93 (54.84%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSLIHDDLP +DNDDLRRGKPTNHK++GE              FEH+   T GV+  R+V A+ E             QV D+   G
Sbjct:  146 VEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKIYGEDVAVLAGDSLLAFAFEHIVNSTAGVTPSRIVGAVAELAKSIGTEGLVAGQVADIKCTG 238

HSP 2 Score: 36.965 bits (84), Expect = 6.417e-22
Identity = 19/27 (70.37%), Postives = 21/27 (77.78%), Query Frame = 3 
Query:  285 VSLSDLEYILVHKTAKLLEASVVCGVI 365
            VSL  LE+I VHKTA LLE+SVV G I
Sbjct:  242 VSLETLEFIHVHKTAALLESSVVLGAI 268
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPPS_SINAL (Geranylgeranyl pyrophosphate synthetase, chloroplastic/chromoplastic OS=Sinapis alba GN=GGPS1 PE=1 SV=1)

HSP 1 Score: 87.8113 bits (216), Expect = 1.507e-20
Identity = 48/94 (51.06%), Postives = 54/94 (57.45%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVG-VSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSLIHDDLP +DNDDLRRGKPTNHKV+GE              FEH+A  T   VS  RVVRA+ E             QV D+ SEG
Sbjct:  140 VEMIHTMSLIHDDLPCMDNDDLRRGKPTNHKVYGEDVAVLAGDALLSFAFEHLASATSSEVSPARVVRAVGELAKAIGTEGLVAGQVVDISSEG 233

HSP 2 Score: 31.5722 bits (70), Expect = 1.507e-20
Identity = 16/28 (57.14%), Postives = 20/28 (71.43%), Query Frame = 3 
Query:  282 DVSLSDLEYILVHKTAKLLEASVVCGVI 365
            +V L  L++I +HKTA LLEAS V G I
Sbjct:  238 NVGLEHLKFIHLHKTAALLEASAVLGGI 265
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPP4_ARATH (Geranylgeranyl pyrophosphate synthetase 4 OS=Arabidopsis thaliana GN=GGPP4 PE=2 SV=1)

HSP 1 Score: 78.5666 bits (192), Expect = 1.960e-20
Identity = 43/93 (46.24%), Postives = 50/93 (53.76%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSLI DDLP +DNDDLRRGKPT HKVFGE              FEH+      VS+ ++VRA+ E             Q  DL SEG
Sbjct:  149 VEMIHTMSLIKDDLPCMDNDDLRRGKPTTHKVFGESVAILSGGALLALAFEHLT--EADVSSKKMVRAVKELAKSIGTKGLVAGQAKDLSSEG 239

HSP 2 Score: 40.4318 bits (93), Expect = 1.960e-20
Identity = 19/30 (63.33%), Postives = 21/30 (70.00%), Query Frame = 3 
Query:  276 RKDVSLSDLEYILVHKTAKLLEASVVCGVI 365
            + DV L DLEYI VHKT  LLEAS V G +
Sbjct:  242 QNDVGLEDLEYIHVHKTGSLLEASAVIGAV 271
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: CRTE_CYAPA (Geranylgeranyl pyrophosphate synthetase homolog OS=Cyanophora paradoxa GN=crtE PE=3 SV=1)

HSP 1 Score: 82.8037 bits (203), Expect = 4.615e-19
Identity = 42/94 (44.68%), Postives = 51/94 (54.26%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEGK 282
            LE  HTMSLIHDDLPA+DND  RRGKPTNH ++GE              FE +A +T  V  D +V+ I +             Q+ DL SEGK
Sbjct:   78 LEMIHTMSLIHDDLPAMDNDSYRRGKPTNHIIYGEDLAILAGDALLAYAFEFIATQTKNVPADLIVKVIAQVAHSVTTSGLVGGQIIDLSSEGK 171

HSP 2 Score: 31.5722 bits (70), Expect = 4.615e-19
Identity = 13/30 (43.33%), Postives = 20/30 (66.67%), Query Frame = 3 
Query:  276 RKDVSLSDLEYILVHKTAKLLEASVVCGVI 365
            + D +L  L +I +HKT  LLEA+V+ G +
Sbjct:  171 KSDTTLETLNFIHIHKTGALLEAAVLSGAL 200
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPP3_ARATH (Geranylgeranyl pyrophosphate synthetase 3, chloroplastic OS=Arabidopsis thaliana GN=GGPP3 PE=2 SV=1)

HSP 1 Score: 76.2554 bits (186), Expect = 6.350e-18
Identity = 41/93 (44.09%), Postives = 52/93 (55.91%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVG-VSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSE 276
            +E  HT SLIHDDLP +DN DLRRGKPTNHKV+GE              FEH+   + G V+ +R++RA+ E             Q+ DL SE
Sbjct:  134 VEMIHTSSLIHDDLPCMDNADLRRGKPTNHKVYGEDMAVLAGDALLALAFEHMTVVSSGLVAPERMIRAVVELARAIGTTGLVAGQMIDLASE 226

HSP 2 Score: 34.2686 bits (77), Expect = 6.350e-18
Identity = 17/27 (62.96%), Postives = 20/27 (74.07%), Query Frame = 3 
Query:  285 VSLSDLEYILVHKTAKLLEASVVCGVI 365
            V L  LE+I +HKTA LLEA+ V GVI
Sbjct:  233 VGLEHLEFIHLHKTAALLEAAAVLGVI 259
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: GGPP2_ARATH (Geranylgeranyl pyrophosphate synthetase 2 OS=Arabidopsis thaliana GN=GGPP2 PE=2 SV=2)

HSP 1 Score: 77.0258 bits (188), Expect = 1.072e-17
Identity = 41/93 (44.09%), Postives = 51/93 (54.84%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFEHVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEG 279
            +E  HTMSLI DDLP +DNDDLRRGKPT HKV+GE              FEH+   T  +S++R+V A+ E             Q  D+ SEG
Sbjct:  153 VEMIHTMSLIKDDLPCMDNDDLRRGKPTTHKVYGEGVAILSGGALLSLAFEHMT--TAEISSERMVWAVRELARSIGTRGLVAGQAMDISSEG 243

HSP 2 Score: 32.7278 bits (73), Expect = 1.072e-17
Identity = 16/28 (57.14%), Postives = 19/28 (67.86%), Query Frame = 3 
Query:  282 DVSLSDLEYILVHKTAKLLEASVVCGVI 365
            +V L  LE+I VHKTA LLE + V G I
Sbjct:  248 EVGLEHLEFIHVHKTAVLLETAAVLGAI 275
[Close]
BLAST of CN184477 vs. ExPASy Swiss-Prot
Match: ISPA_BACSU (Geranyltranstransferase OS=Bacillus subtilis GN=ispA PE=3 SV=2)

HSP 1 Score: 72.4034 bits (176), Expect = 1.281e-14
Identity = 40/98 (40.82%), Postives = 54/98 (55.10%), Query Frame = 1 
Query:    1 LEXTHTMSLIHDDLPALDNDDLRRGKPTNHKVFGERTXXXXXXXXXXXXFE----HVAPKTVGVSTDRVVRAITEXXXXXXXXXXXXXQVTDLDSEGK 282
            +E  HT SLIHDDLP +D+DDLRRGKPTNHKVFGE T            F+    HV+ +   VS ++ +R + E             QV D+++E +
Sbjct:   74 VEMIHTYSLIHDDLPCMDDDDLRRGKPTNHKVFGEATAVLAGDGLLTESFKLITSHVSDE---VSAEKRLRLVNELISAAGTEGMVGGQVADMEAENR 168

HSP 2 Score: 26.9498 bits (58), Expect = 1.281e-14
Identity = 14/29 (48.28%), Postives = 18/29 (62.07%), Query Frame = 3 
Query:  279 KDVSLSDLEYILVHKTAKLLEASVVCGVI 365
            + V+L +LE I   KTAKLL   V+ G I
Sbjct:  168 RQVTLEELESIHERKTAKLLGFCVIAGAI 196
The following BLAST results are available for this feature:
BLAST of CN184477 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
GGPPS_CATRO4.318e-2450.54Geranylgeranyl pyrophosphate synthetase, chloropla... [more]
GGPPS_HEVBR1.237e-2350.54Geranylgeranyl pyrophosphate synthetase, chloropla... [more]
GGPP1_ARATH4.932e-2251.58Geranylgeranyl pyrophosphate synthetase 1, chlorop... [more]
GGPPS_CAPAN6.417e-2245.16Geranylgeranyl pyrophosphate synthetase, chloropla... [more]
GGPPS_SINAL1.507e-2051.06Geranylgeranyl pyrophosphate synthetase, chloropla... [more]
GGPP4_ARATH1.960e-2046.24Geranylgeranyl pyrophosphate synthetase 4 OS=Arabi... [more]
CRTE_CYAPA4.615e-1944.68Geranylgeranyl pyrophosphate synthetase homolog OS... [more]
GGPP3_ARATH6.350e-1844.09Geranylgeranyl pyrophosphate synthetase 3, chlorop... [more]
GGPP2_ARATH1.072e-1744.09Geranylgeranyl pyrophosphate synthetase 2 OS=Arabi... [more]
ISPA_BACSU1.281e-1440.82Geranyltranstransferase OS=Bacillus subtilis GN=is... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0005J05_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005J05, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184477 ID=CN184477; Name=CN184477; organism=Citrus sinensis; type=EST; length=366bp
CTAGAGANGACTCACACGATGTCCCTCATTCATGATGATCTCCCTGCACT
GGACAACGATGATCTTAGACGAGGAAAGCCTACAAATCACAAAGTCTTTG
GTGAACGAACCGCAATTCTTGCAGGTGATGCTCTGCTTGCCCTTGCATTT
GAACATGTAGCACCCAAGACCGTTGGTGTGTCAACTGACCGCGTGGTTCG
AGCCATTACGGAGCTGGGTTCCGCACTTGGAGCAAAAGGGCTCCTGAAAG
GACAAGTTACTGACCTTGACAGTGAAGGAAAGATGTGAGTTTGAGTGATT
TGGAGTACATTCTTGTGCACAAAACCGCGAAGCTTTTAGAGGCATCTGTT
GTTTGCGGAGTGATAA
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