CN184382

Overview
NameCN184382
Unique NameCN184382
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length532
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_ARATH (Aminomethyltransferase, mitochondrial OS=Arabidopsis thaliana GN=GDCST PE=1 SV=1)

HSP 1 Score: 162.54 bits (410), Expect = 9.334e-46
Identity = 77/96 (80.21%), Postives = 85/96 (88.54%), Query Frame = -3 
Query:  138 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYKPS 425
            IL+QL +GP +RRVGFF+ GPPARSHS+VHDE GN IGEITSGGFSP LKKNIAMGYVKSG HK GTKVKI VRGK Y+G+ITKMPFV TKYYKP+
Sbjct:  313 ILQQLKDGPTIRRVGFFSSGPPARSHSEVHDESGNKIGEITSGGFSPNLKKNIAMGYVKSGQHKTGTKVKILVRGKPYEGSITKMPFVATKYYKPT 408

HSP 2 Score: 40.817 bits (94), Expect = 9.334e-46
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH++PVEAGLTWAIGKRR +
Sbjct:  283 EQHISPVEAGLTWAIGKRRRA 303
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_SOLTU (Aminomethyltransferase, mitochondrial OS=Solanum tuberosum GN=GDCST PE=2 SV=1)

HSP 1 Score: 161.384 bits (407), Expect = 1.585e-45
Identity = 73/95 (76.84%), Postives = 83/95 (87.37%), Query Frame = -3 
Query:  141 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYKP 425
            ILKQ++EGP +RRVGFF+ GPP RSHS++ D  G  IGEITSGGFSPCLKKNIAMGYVK+G HKAGT VKI +RGK+YDG +TKMPFVPTKYYKP
Sbjct:  312 ILKQIEEGPKIRRVGFFSSGPPPRSHSEIQDSNGQNIGEITSGGFSPCLKKNIAMGYVKTGNHKAGTNVKIVIRGKSYDGVVTKMPFVPTKYYKP 406

HSP 2 Score: 41.2022 bits (95), Expect = 1.585e-45
Identity = 17/21 (80.95%), Postives = 19/21 (90.48%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH TPVEAGLTWAIGKRR +
Sbjct:  282 EQHTTPVEAGLTWAIGKRRRA 302
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_FLATR (Aminomethyltransferase, mitochondrial OS=Flaveria trinervia GN=GDCST PE=3 SV=1)

HSP 1 Score: 160.229 bits (404), Expect = 1.585e-45
Identity = 71/96 (73.96%), Postives = 85/96 (88.54%), Query Frame = -3 
Query:  138 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYKPS 425
            ILKQ+ +GP +RRVG F+ GPPARSHS++ +EQG  IGE+TSGGFSPCLKKNI MGYVKSGLHK GTK+KI +RGK Y+G++TKMPFVPTKYYKP+
Sbjct:  312 ILKQIADGPAIRRVGLFSTGPPARSHSEIQNEQGENIGEVTSGGFSPCLKKNIGMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMPFVPTKYYKPA 407

HSP 2 Score: 42.3578 bits (98), Expect = 1.585e-45
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH+TPVEAGLTWAIGKRR +
Sbjct:  282 EQHITPVEAGLTWAIGKRRRA 302
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_PEA (Aminomethyltransferase, mitochondrial OS=Pisum sativum GN=GDCST PE=1 SV=2)

HSP 1 Score: 159.844 bits (403), Expect = 2.692e-45
Identity = 74/96 (77.08%), Postives = 84/96 (87.50%), Query Frame = -3 
Query:  138 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYKPS 425
            ILKQL +GP +RRVGF + GPP RSHS++ DE GN IGE+TSGGFSPCLKKNIA+GYVKSGLHKAGTKVKI +RGK  +G +TKMPFVPTKYYKPS
Sbjct:  313 ILKQLADGPSIRRVGFISSGPPPRSHSEIQDEGGNNIGEVTSGGFSPCLKKNIAIGYVKSGLHKAGTKVKIIIRGKQNEGVVTKMPFVPTKYYKPS 408

HSP 2 Score: 41.9726 bits (97), Expect = 2.692e-45
Identity = 16/21 (76.19%), Postives = 20/21 (95.24%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH+TP+EAGLTWAIGKRR +
Sbjct:  283 EQHITPIEAGLTWAIGKRRRA 303
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_FLAPR (Aminomethyltransferase, mitochondrial OS=Flaveria pringlei GN=GDCST PE=2 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.573e-45
Identity = 70/96 (72.92%), Postives = 85/96 (88.54%), Query Frame = -3 
Query:  138 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYKPS 425
            ILKQ+ +GP +RRVG F+ GPPARSHS++ +E+G  IGE+TSGGFSPCLKKNI MGYVKSGLHK GTK+KI +RGK Y+G++TKMPFVPTKYYKP+
Sbjct:  312 ILKQIADGPAIRRVGLFSTGPPARSHSEIQNEKGENIGEVTSGGFSPCLKKNIGMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMPFVPTKYYKPA 407

HSP 2 Score: 42.3578 bits (98), Expect = 4.573e-45
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH+TPVEAGLTWAIGKRR +
Sbjct:  282 EQHITPVEAGLTWAIGKRRRA 302
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_FLAAN (Aminomethyltransferase, mitochondrial OS=Flaveria anomala GN=GDCST PE=3 SV=1)

HSP 1 Score: 158.688 bits (400), Expect = 4.573e-45
Identity = 70/96 (72.92%), Postives = 85/96 (88.54%), Query Frame = -3 
Query:  138 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYKPS 425
            ILKQ+ +GP +RRVG F+ GPPARSHS++ +E+G  IGE+TSGGFSPCLKKNI MGYVKSGLHK GTK+KI +RGK Y+G++TKMPFVPTKYYKP+
Sbjct:  312 ILKQIADGPAIRRVGLFSTGPPARSHSEIQNEKGENIGEVTSGGFSPCLKKNIGMGYVKSGLHKPGTKLKIVIRGKTYEGSVTKMPFVPTKYYKPA 407

HSP 2 Score: 42.3578 bits (98), Expect = 4.573e-45
Identity = 17/21 (80.95%), Postives = 20/21 (95.24%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH+TPVEAGLTWAIGKRR +
Sbjct:  282 EQHITPVEAGLTWAIGKRRRA 302
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_MESCR (Aminomethyltransferase, mitochondrial OS=Mesembryanthemum crystallinum GN=GDCST PE=2 SV=1)

HSP 1 Score: 154.836 bits (390), Expect = 1.097e-43
Identity = 72/94 (76.60%), Postives = 81/94 (86.17%), Query Frame = -3 
Query:  144 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYK 425
            ILKQ+ +GPP RRVGF + GPPAR HS++ +E+G  IGEITSGGFSPCLKKNIAMGYVKSG HKAGTKV I VRGK Y+G +TKMPFVPTKYYK
Sbjct:  313 ILKQIADGPPQRRVGFISSGPPARGHSEIQNEKGESIGEITSGGFSPCLKKNIAMGYVKSGNHKAGTKVNILVRGKPYEGVVTKMPFVPTKYYK 406

HSP 2 Score: 41.5874 bits (96), Expect = 1.097e-43
Identity = 15/21 (71.43%), Postives = 20/21 (95.24%), Query Frame = -2 
Query:  454 KQHVTPVEAGLTWAIGKRRES 516
            +QH+TP+EAGLTWA+GKRR +
Sbjct:  283 EQHITPIEAGLTWAVGKRRRA 303
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_CHICK (Aminomethyltransferase, mitochondrial OS=Gallus gallus GN=AMT PE=2 SV=2)

HSP 1 Score: 90.5077 bits (223), Expect = 1.496e-21
Identity = 44/93 (47.31%), Postives = 61/93 (65.59%), Query Frame = -3 
Query:  147 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYY 425
            I++Q+ E P  +RVG  + GPP R  + +   +G P+G +TSG  SP L KNIAMGYV++   + GT + +EVR K +   +TKMPFVPT YY
Sbjct:  297 IMEQVKEKPKRKRVGLTSVGPPLRPPAAILGPEGTPVGTVTSGCPSPSLGKNIAMGYVQAAHSRPGTTLTVEVRKKQHPALVTKMPFVPTHYY 389

HSP 2 Score: 31.5722 bits (70), Expect = 1.496e-21
Identity = 12/20 (60.00%), Postives = 15/20 (75.00%), Query Frame = -2 
Query:  454 QHVTPVEAGLTWAIGKRRES 513
            +  TPVEAGL W +GKRR +
Sbjct:  268 ESTTPVEAGLLWTLGKRRRT 287
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_SCHPO (Probable aminomethyltransferase, mitochondrial OS=Schizosaccharomyces pombe GN=gcv1 PE=2 SV=1)

HSP 1 Score: 94.3597 bits (233), Expect = 3.295e-21
Identity = 47/95 (49.47%), Postives = 61/95 (64.21%), Query Frame = -3 
Query:  144 KILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYYK 428
            +ILK+L +GP  RRVGF     PAR H    +  G  +G++TSG  SP L KNIAMGY+ +GLH+ GT   I+VR K +   + +MPFV T YYK
Sbjct:  294 RILKELKDGPSRRRVGFIVEKVPAR-HGSAVEVDGVEVGQVTSGCPSPTLGKNIAMGYISTGLHQVGTPAHIKVRNKLHPAQVVRMPFVETHYYK 387

HSP 2 Score: 26.5646 bits (57), Expect = 3.295e-21
Identity = 10/15 (66.67%), Postives = 12/15 (80.00%), Query Frame = -2 
Query:  460 TPVEAGLTWAIGKRR 504
            +PVE  L+W IGKRR
Sbjct:  269 SPVEGSLSWIIGKRR 283
[Close]
BLAST of CN184382 vs. ExPASy Swiss-Prot
Match: GCST_CANFA (Aminomethyltransferase, mitochondrial OS=Canis familiaris GN=AMT PE=3 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.074e-20
Identity = 45/93 (48.39%), Postives = 57/93 (61.29%), Query Frame = -3 
Query:  147 ILKQLDEGPPVRRVGFFAGGPPARSHSKVHDEQGNPIGEITSGGFSPCLKKNIAMGYVKSGLHKAGTKVKIEVRGKAYDGNITKMPFVPTKYY 425
            I+ QL      RRVG    G P R+HS + + +G  IG +TSG  SPCLKKN+AMGYV S   + GT + +EVR K     ++KMPFV T YY
Sbjct:  308 IIAQLKGKVQRRRVGLTCEGAPVRAHSPILNMEGTVIGTVTSGCPSPCLKKNVAMGYVPSEYSRPGTPLLVEVRRKQQMAVVSKMPFVTTNYY 400

HSP 2 Score: 31.9574 bits (71), Expect = 2.074e-20
Identity = 11/20 (55.00%), Postives = 15/20 (75.00%), Query Frame = -2 
Query:  454 QHVTPVEAGLTWAIGKRRES 513
            +H TPVE  L+W +GKRR +
Sbjct:  279 EHTTPVEGSLSWTLGKRRRA 298
The following BLAST results are available for this feature:
BLAST of CN184382 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 17
Match NameE-valueIdentityDescription
GCST_ARATH9.334e-4680.21Aminomethyltransferase, mitochondrial OS=Arabidops... [more]
GCST_SOLTU1.585e-4576.84Aminomethyltransferase, mitochondrial OS=Solanum t... [more]
GCST_FLATR1.585e-4573.96Aminomethyltransferase, mitochondrial OS=Flaveria ... [more]
GCST_PEA2.692e-4577.08Aminomethyltransferase, mitochondrial OS=Pisum sat... [more]
GCST_FLAPR4.573e-4572.92Aminomethyltransferase, mitochondrial OS=Flaveria ... [more]
GCST_FLAAN4.573e-4572.92Aminomethyltransferase, mitochondrial OS=Flaveria ... [more]
GCST_MESCR1.097e-4376.60Aminomethyltransferase, mitochondrial OS=Mesembrya... [more]
GCST_CHICK1.496e-2147.31Aminomethyltransferase, mitochondrial OS=Gallus ga... [more]
GCST_SCHPO3.295e-2149.47Probable aminomethyltransferase, mitochondrial OS=... [more]
GCST_CANFA2.074e-2048.39Aminomethyltransferase, mitochondrial OS=Canis fam... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0005G14_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005G14, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184382 ID=CN184382; Name=CN184382; organism=Citrus sinensis; type=EST; length=532bp
CAAGAATCATAAGAATTTGCAGAATTAAAGAACTGCTAGCAATAAAGACA
GTAATCTCGAGCAGTCAATGTTTATGAAACAAGTCAAAACAGGAATCGAG
TAGAGCTTCCATAAGCAGAACCAACAAAGATGTACTAAGACGGCTTGTAG
TATTTTGTTGGCACAAATGGCATTTTAGTGATGTTTCCATCGTAGGCTTT
TCCTCGAACCTCAATCTTAACTTTTGTGCCTGCCTTGTGCAATCCAGATT
TTACATATCCCATGGCTATATTCTTCTTGAGGCAGGGGCTGAATCCTCCA
CTGGTAATTTCTCCAATGGGATTCCCTTGCTCATCGTGAACCTTACTGTG
TGATCTGGCAGGCGGACCTCCAGCGAAGAATCCAACACGTCTGACTGGCG
GACCTTCGTCAAGTTGTTTGAGTATTTTCTCAGCACCTAGAAAGCCACCT
TCTGCTCTCCCTCCTCTTTCCTATGGCCCATGTCAGTCCAGCTTCTACAG
GTGTCACGTGTTGTTTCCATGTCATTACCATA
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