CN184199

Overview
NameCN184199
Unique NameCN184199
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length754
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B2_ARATH (Transcription initiation factor IIB-2 OS=Arabidopsis thaliana GN=TFIIB2 PE=2 SV=1)

HSP 1 Score: 381.333 bits (978), Expect = 3.004e-105
Identity = 184/193 (95.34%), Postives = 193/193 (100.00%), Query Frame = 1 
Query:  175 MTDAFCSDCKKHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ 753
            M+DAFCSDCK+HTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGL+TVI+KPNG+SG+FLSSSLGRWQNRGSNPDRGLI+AFKTIATM+DRLGLVATIKDRANEIYK+VEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ
Sbjct:    1 MSDAFCSDCKRHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLTTVISKPNGSSGDFLSSSLGRWQNRGSNPDRGLIVAFKTIATMADRLGLVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ 193
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_SOYBN (Transcription initiation factor IIB OS=Glycine max GN=TFIIB1 PE=2 SV=1)

HSP 1 Score: 367.081 bits (941), Expect = 5.863e-101
Identity = 182/194 (93.81%), Postives = 186/194 (95.88%), Query Frame = 1 
Query:  175 MTDAFCSDCKKHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASG-EFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ 753
            M+DAFCSDCK+ TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDP RVGGP+NPLL DGGLSTVIAKPNG  G EFLSSSLGRWQNRGSNPDR LI AFKTIATMSDRLGLVATIKDRANEIYK+VEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ
Sbjct:    1 MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPNRVGGPSNPLLTDGGLSTVIAKPNGGGGGEFLSSSLGRWQNRGSNPDRALIQAFKTIATMSDRLGLVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ 194
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_ORYSJ (Transcription initiation factor IIB OS=Oryza sativa subsp. japonica GN=TFIIB PE=1 SV=1)

HSP 1 Score: 362.844 bits (930), Expect = 1.106e-99
Identity = 174/193 (90.16%), Postives = 184/193 (95.34%), Query Frame = 1 
Query:  175 MTDAFCSDCKKHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVKQ 753
            M+D+FC DCKKHTEV FDHSAGDTVC+ECGLVLE+HS+DETSEWRTFANES DNDPVRVGGPTNPLL DGGLSTVIAKPNGA GEFLSSSLGRWQNRGSNPDR LILAF+TIA M+DRLGLVATIKDRANEIYKKVED KS RGRNQDA+LAACLYIACRQED+PRTVKEICSVANGATKKEIGRAKE+IVKQ
Sbjct:    1 MSDSFCPDCKKHTEVAFDHSAGDTVCTECGLVLEAHSVDETSEWRTFANESSDNDPVRVGGPTNPLLTDGGLSTVIAKPNGAQGEFLSSSLGRWQNRGSNPDRSLILAFRTIANMADRLGLVATIKDRANEIYKKVEDLKSIRGRNQDAILAACLYIACRQEDRPRTVKEICSVANGATKKEIGRAKEFIVKQ 193
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B1_ARATH (Transcription initiation factor IIB-1 OS=Arabidopsis thaliana GN=TFIIB1 PE=1 SV=1)

HSP 1 Score: 342.813 bits (878), Expect = 1.184e-93
Identity = 164/192 (85.42%), Postives = 181/192 (94.27%), Query Frame = 1 
Query:  175 MTDAFCSDCKKHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            M+DA+C+DCKK TE+V DHSAGDT+CSECGLVLESHSIDETSEWRTFANES ++DP RVGGPTNPLLAD  L+TVIAKPNG+SG+FLSSSLGRWQNR SN DRGLI AFKTIATMS+RLGLVATIKDRANE+YK++EDQKSSRGRNQDAL AACLYIACRQEDKPRT+KEIC +ANGATKKEIGRAK+YIVK
Sbjct:    1 MSDAYCTDCKKETELVVDHSAGDTLCSECGLVLESHSIDETSEWRTFANESSNSDPNRVGGPTNPLLADSALTTVIAKPNGSSGDFLSSSLGRWQNRNSNSDRGLIQAFKTIATMSERLGLVATIKDRANELYKRLEDQKSSRGRNQDALYAACLYIACRQEDKPRTIKEICVIANGATKKEIGRAKDYIVK 192
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_DROME (Transcription initiation factor IIB OS=Drosophila melanogaster GN=TfIIB PE=1 SV=1)

HSP 1 Score: 179.874 bits (455), Expect = 1.328e-44
Identity = 98/188 (52.13%), Postives = 126/188 (67.02%), Query Frame = 1 
Query:  199 CKKHTE--VVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRG--SNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            C  H E  ++ D+ AGD +CSECGLV+    ID  SEWRTF+NE    DP RVGGP NPLL+ G LST+I  P   S  F +    ++QNR   S+ DR LI AFK I++M+DR+ L  TI DRAN ++K+V D K+ +GR+ DA  +ACLYIACRQE  PRT KEIC+V+   +KKEIGR  +  +K
Sbjct:   14 CYAHPESPLIEDYRAGDMICSECGLVVGDRVIDVGSEWRTFSNEKSGVDPSRVGGPENPLLSGGDLSTIIG-PGTGSASFDAFGAPKYQNRRTMSSSDRSLISAFKEISSMADRINLPKTIVDRANNLFKQVHDGKNLKGRSNDAKASACLYIACRQEGVPRTFKEICAVSK-ISKKEIGRCFKLTLK 199
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_RAT (Transcription initiation factor IIB OS=Rattus norvegicus GN=Gtf2b PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 3.270e-43
Identity = 94/188 (50.00%), Postives = 123/188 (65.43%), Query Frame = 1 
Query:  199 CKKHTEVVF--DHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRG--SNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            C  H + +   D+ AGD +C ECGLV+    ID  SEWRTF+N+    DP RVG   NPLL+DG LST+I K  GA+  F      ++QNR   S+ DR ++ AFK I TM+DR+ L   I DR N ++K+V +QKS +GR  DA+ +ACLYIACRQE  PRT KEIC+V+   +KKEIGR  + I+K
Sbjct:   15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAAS-FDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSR-ISKKEIGRCFKLILK 200
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_PONAB (Transcription initiation factor IIB OS=Pongo abelii GN=GTF2B PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 3.270e-43
Identity = 94/188 (50.00%), Postives = 123/188 (65.43%), Query Frame = 1 
Query:  199 CKKHTEVVF--DHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRG--SNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            C  H + +   D+ AGD +C ECGLV+    ID  SEWRTF+N+    DP RVG   NPLL+DG LST+I K  GA+  F      ++QNR   S+ DR ++ AFK I TM+DR+ L   I DR N ++K+V +QKS +GR  DA+ +ACLYIACRQE  PRT KEIC+V+   +KKEIGR  + I+K
Sbjct:   15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAAS-FDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSR-ISKKEIGRCFKLILK 200
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_MOUSE (Transcription initiation factor IIB OS=Mus musculus GN=Gtf2b PE=1 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 3.270e-43
Identity = 94/188 (50.00%), Postives = 123/188 (65.43%), Query Frame = 1 
Query:  199 CKKHTEVVF--DHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRG--SNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            C  H + +   D+ AGD +C ECGLV+    ID  SEWRTF+N+    DP RVG   NPLL+DG LST+I K  GA+  F      ++QNR   S+ DR ++ AFK I TM+DR+ L   I DR N ++K+V +QKS +GR  DA+ +ACLYIACRQE  PRT KEIC+V+   +KKEIGR  + I+K
Sbjct:   15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAAS-FDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSR-ISKKEIGRCFKLILK 200
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_MACFA (Transcription initiation factor IIB OS=Macaca fascicularis GN=GTF2B PE=2 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 3.270e-43
Identity = 94/188 (50.00%), Postives = 123/188 (65.43%), Query Frame = 1 
Query:  199 CKKHTEVVF--DHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRG--SNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            C  H + +   D+ AGD +C ECGLV+    ID  SEWRTF+N+    DP RVG   NPLL+DG LST+I K  GA+  F      ++QNR   S+ DR ++ AFK I TM+DR+ L   I DR N ++K+V +QKS +GR  DA+ +ACLYIACRQE  PRT KEIC+V+   +KKEIGR  + I+K
Sbjct:   15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAAS-FDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSR-ISKKEIGRCFKLILK 200
[Close]
BLAST of CN184199 vs. ExPASy Swiss-Prot
Match: TF2B_HUMAN (Transcription initiation factor IIB OS=Homo sapiens GN=GTF2B PE=1 SV=1)

HSP 1 Score: 175.252 bits (443), Expect = 3.270e-43
Identity = 94/188 (50.00%), Postives = 123/188 (65.43%), Query Frame = 1 
Query:  199 CKKHTEVVF--DHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRG--SNPDRGLILAFKTIATMSDRLGLVATIKDRANEIYKKVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATKKEIGRAKEYIVK 750
            C  H + +   D+ AGD +C ECGLV+    ID  SEWRTF+N+    DP RVG   NPLL+DG LST+I K  GA+  F      ++QNR   S+ DR ++ AFK I TM+DR+ L   I DR N ++K+V +QKS +GR  DA+ +ACLYIACRQE  PRT KEIC+V+   +KKEIGR  + I+K
Sbjct:   15 CPNHPDAILVEDYRAGDMICPECGLVVGDRVIDVGSEWRTFSNDKATKDPSRVGDSQNPLLSDGDLSTMIGKGTGAAS-FDEFGNSKYQNRRTMSSSDRAMMNAFKEITTMADRINLPRNIVDRTNNLFKQVYEQKSLKGRANDAIASACLYIACRQEGVPRTFKEICAVSR-ISKKEIGRCFKLILK 200
The following BLAST results are available for this feature:
BLAST of CN184199 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 67
Match NameE-valueIdentityDescription
TF2B2_ARATH3.004e-10595.34Transcription initiation factor IIB-2 OS=Arabidops... [more]
TF2B_SOYBN5.863e-10193.81Transcription initiation factor IIB OS=Glycine max... [more]
TF2B_ORYSJ1.106e-9990.16Transcription initiation factor IIB OS=Oryza sativ... [more]
TF2B1_ARATH1.184e-9385.42Transcription initiation factor IIB-1 OS=Arabidops... [more]
TF2B_DROME1.328e-4452.13Transcription initiation factor IIB OS=Drosophila ... [more]
TF2B_RAT3.270e-4350.00Transcription initiation factor IIB OS=Rattus norv... [more]
TF2B_PONAB3.270e-4350.00Transcription initiation factor IIB OS=Pongo abeli... [more]
TF2B_MOUSE3.270e-4350.00Transcription initiation factor IIB OS=Mus musculu... [more]
TF2B_MACFA3.270e-4350.00Transcription initiation factor IIB OS=Macaca fasc... [more]
TF2B_HUMAN3.270e-4350.00Transcription initiation factor IIB OS=Homo sapien... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0005C10_r Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0005C10, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN184199 ID=CN184199; Name=CN184199; organism=Citrus sinensis; type=EST; length=754bp
AGGCTCTTTCTTCCTCTCTTCATTTCTTCACCTAAAAAGTTTCGCACAAC
AATTCCACCGGAATCCCAAAGAACAGGGCTATAATATTAAATTAAAATTA
AAAATTTAAATAATTTTAAAAAATTAAAAGAAGAAACGGAAATACGTAGG
GGATTAAATTAAATCGAAGGGGAAATGACGGACGCCTTTTGCTCGGACTG
CAAGAAGCACACGGAGGTGGTGTTCGACCACTCGGCGGGGGACACGGTGT
GCTCGGAGTGCGGCCTCGTGTTGGAGTCGCACTCCATCGACGAGACCTCC
GAGTGGCGTACCTTCGCCAACGAATCCGGCGACAACGACCCCGTCCGTGT
CGGCGGCCCCACCAACCCGCTCCTGGCCGACGGCGGCCTCTCCACCGTCA
TCGCCAAGCCTAATGGAGCTTCCGGAGAATTCTTGTCGTCGTCGCTCGGA
CGCTGGCAGAATCGCGGCTCGAATCCGGATCGCGGCTTGATTTTGGCTTT
TAAAACAATTGCTACCATGTCTGATAGGTTAGGCCTCGTTGCAACCATTA
AGGATCGGGCAAATGAGATATATAAGAAGGTGGAAGATCAAAAGTCTAGT
AGAGGCAGAAATCAAGACGCATTATTGGCTGCTTGCCTGTACATTGCTTG
TCGACAAGAAGACAAGCCACGCACTGTTAAGGAAATCTGCTCTGTCGCCA
ATGGAGCTACAAAGAAGGAAATTGGCCGAGCAAAAGAATACATTGTGAAA
CAGC
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