EY666315

Overview
NameEY666315
Unique NameEY666315
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length870
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC2_PEA (Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pisum sativum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 141.354 bits (355), Expect = 6.552e-33
Identity = 91/177 (51.41%), Postives = 106/177 (59.89%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAILD-IPALSQKKGLWFKGRDP-PGTCQKVPNACKWAKKLTLLL--KGGVPLKWN-PGGPPGGLKRENPRGW-GAPHGGEG 521
            M DFLKAL+KRLK N  AYENYHRI+VP+G P K   KEPLRVNV+FQHIQKMLSSETAVIAETGDSWF  QKLKLP   G       G IGW+VGA L    A+ +K+ +   G      T Q V    +  +K  + L   GG  ++     GP   +K  N  G   A H GEG
Sbjct:  167 MNDFLKALAKRLKHNNVAYENYHRIFVPDGTPLKSASKEPLRVNVMFQHIQKMLSSETAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIACIGDGSFQVTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEG 343
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC1_TOBAC (Pyruvate decarboxylase isozyme 1 (Fragment) OS=Nicotiana tabacum GN=PDC1 PE=2 SV=1)

HSP 1 Score: 140.584 bits (353), Expect = 1.118e-32
Identity = 71/108 (65.74%), Postives = 80/108 (74.07%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAILDIPALSQKK 332
            M+DFL AL+KRLK N TA+ENYHRIYVPEG P KCEPKE LRVNVLFQHIQ MLS ++ VIAETGDSWF  QKLKLP+  G       G IGW+VGA L     + +K
Sbjct:  272 MRDFLAALAKRLKHNPTAFENYHRIYVPEGHPLKCEPKEALRVNVLFQHIQNMLSGDSVVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAAPEK 379
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC2_ORYSJ (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. japonica GN=PDC2 PE=2 SV=1)

HSP 1 Score: 134.42 bits (337), Expect = 8.009e-31
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAIL 305
            MK+FL  L+KR+  NTTAYENY RI+VPEGQP + EP EPLRVNVLF+H+QKML+S++AVIAETGDSWF  QKLKLP   G       G IGW+VGA+L
Sbjct:  368 MKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALL 466
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC2_ORYSI (Pyruvate decarboxylase isozyme 2 OS=Oryza sativa subsp. indica GN=PDC2 PE=2 SV=2)

HSP 1 Score: 134.42 bits (337), Expect = 8.009e-31
Identity = 66/99 (66.67%), Postives = 78/99 (78.79%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAIL 305
            MK+FL  L+KR+  NTTAYENY RI+VPEGQP + EP EPLRVNVLF+H+QKML+S++AVIAETGDSWF  QKLKLP   G       G IGW+VGA+L
Sbjct:  368 MKEFLSELAKRVNKNTTAYENYKRIFVPEGQPLESEPNEPLRVNVLFKHVQKMLNSDSAVIAETGDSWFNCQKLKLPEGCGYEFQMQYGSIGWSVGALL 466
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC2_TOBAC (Pyruvate decarboxylase isozyme 2 OS=Nicotiana tabacum GN=PDC2 PE=2 SV=1)

HSP 1 Score: 132.88 bits (333), Expect = 2.330e-30
Identity = 93/203 (45.81%), Postives = 107/203 (52.71%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*-TSNAL--------------------------GIIGWAVGAILDIPALSQKKGLWFKGRDP--PGTCQKVPN--ACKWAKKLTLLLKGGVPLKWN-PGGPPGGLKRENPRGW-GAPHGGEG 521
            MKDFL  L+K++K N TAYENY RI+VPEG P K EP EPLRVNVLFQHIQKMLS ETAVIAETGDSWF  QKLKLP   G  T+N+L                          G IGW+VGA L       KK +     D     T Q V     C+    + L+  GG  ++     GP   +K  N  G   A H GEG
Sbjct:  350 MKDFLSELAKKIKKNETAYENYRRIFVPEGTPLKSEPNEPLRVNVLFQHIQKMLSDETAVIAETGDSWFNCQKLKLPEGCGYVTNNSLSAWYPFYLQTLEEKSSCCRYEFQMQYGSIGWSVGATLGYAQSVPKKRVISCIGDGSFQVTAQDVSTMIRCEQKNIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGEG 552
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC1_ORYSJ (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. japonica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 6.346e-28
Identity = 61/108 (56.48%), Postives = 78/108 (72.22%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAILDIPALSQKK 332
            M +FL AL+KRL  NTTAY+NY RI++P+ +PP  +P EPLRVN+LF+HI++MLS +TAVIAETGDSWF  QKL+LP   G       G IGW+VGA L     ++ K
Sbjct:  367 MTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDK 474
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC1_ORYSI (Pyruvate decarboxylase isozyme 1 OS=Oryza sativa subsp. indica GN=PDC1 PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 6.346e-28
Identity = 61/108 (56.48%), Postives = 78/108 (72.22%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAILDIPALSQKK 332
            M +FL AL+KRL  NTTAY+NY RI++P+ +PP  +P EPLRVN+LF+HI++MLS +TAVIAETGDSWF  QKL+LP   G       G IGW+VGA L     ++ K
Sbjct:  367 MTEFLDALAKRLDRNTTAYDNYRRIFIPDREPPNGQPDEPLRVNILFKHIKEMLSGDTAVIAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDK 474
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC1_MAIZE (Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PDC1 PE=2 SV=1)

HSP 1 Score: 124.79 bits (312), Expect = 6.346e-28
Identity = 61/108 (56.48%), Postives = 79/108 (73.15%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAILDIPALSQKK 332
            M +FL+AL+KRL+ NTTAY+NY RI+VP+ +PP  +P EPLRVNVLF+HI+ MLS ++AV+AETGDSWF  QKL+LP   G       G IGW+VGA L     ++ K
Sbjct:  372 MPEFLRALAKRLRRNTTAYDNYRRIFVPDREPPNGKPNEPLRVNVLFKHIKGMLSGDSAVVAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGATLGYAQAAKDK 479
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC1_PEA (Pyruvate decarboxylase isozyme 1 OS=Pisum sativum GN=PDC1 PE=2 SV=1)

HSP 1 Score: 122.094 bits (305), Expect = 4.113e-27
Identity = 63/99 (63.64%), Postives = 73/99 (73.74%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSETAVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAIL 305
            M DFL AL+K++K+NTTA ENY RIYVP G P K E  EPLRVNVLF+HIQ ++S +TAVIAETGDSWF  QKL+LP   G       G IGW+VGA L
Sbjct:  355 MADFLTALAKKVKTNTTAVENYRRIYVPPGIPLKREKDEPLRVNVLFKHIQALISGDTAVIAETGDSWFNCQKLRLPENCGYEFQMQYGSIGWSVGATL 453
[Close]
BLAST of EY666315 vs. ExPASy Swiss-Prot
Match: PDC3_ORYSJ (Pyruvate decarboxylase isozyme 3 OS=Oryza sativa subsp. japonica GN=PDC3 PE=2 SV=1)

HSP 1 Score: 119.013 bits (297), Expect = 3.482e-26
Identity = 61/110 (55.45%), Postives = 77/110 (70.00%), Query Frame = 3 
Query:   12 MKDFLKALSKRLKSNTTAYENYHRIYVPEGQPPKCEPKEPLRVNVLFQHIQKMLSSET--AVIAETGDSWFXLQKLKLPRXAG*T-SNALGIIGWAVGAILDIPALSQKK 332
            M+DFL  L+KR++ NTTA++NY RI+VPEGQ P+CE  E LRVNVLF+HIQ+M+      AV+AETGDSWF  QKL+LP   G       G IGW+VGA+L      QK+
Sbjct:  348 MRDFLSELAKRVRKNTTAFDNYKRIFVPEGQLPECEAGEALRVNVLFKHIQRMIGGTEIGAVMAETGDSWFNCQKLRLPEGCGYEFQMQYGSIGWSVGALLGYAQAVQKR 457
The following BLAST results are available for this feature:
BLAST of EY666315 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
PDC2_PEA6.552e-3351.41Pyruvate decarboxylase isozyme 2 (Fragment) OS=Pis... [more]
PDC1_TOBAC1.118e-3265.74Pyruvate decarboxylase isozyme 1 (Fragment) OS=Nic... [more]
PDC2_ORYSJ8.009e-3166.67Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s... [more]
PDC2_ORYSI8.009e-3166.67Pyruvate decarboxylase isozyme 2 OS=Oryza sativa s... [more]
PDC2_TOBAC2.330e-3045.81Pyruvate decarboxylase isozyme 2 OS=Nicotiana taba... [more]
PDC1_ORYSJ6.346e-2856.48Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s... [more]
PDC1_ORYSI6.346e-2856.48Pyruvate decarboxylase isozyme 1 OS=Oryza sativa s... [more]
PDC1_MAIZE6.346e-2856.48Pyruvate decarboxylase isozyme 1 OS=Zea mays GN=PD... [more]
PDC1_PEA4.113e-2763.64Pyruvate decarboxylase isozyme 1 OS=Pisum sativum ... [more]
PDC3_ORYSJ3.482e-2655.45Pyruvate decarboxylase isozyme 3 OS=Oryza sativa s... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-021-E07-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY666315 ID=EY666315; Name=EY666315; organism=Citrus sinensis; type=EST; length=870bp
CCACGCGTCCGATGAAGGATTTCCTCAAAGCACTTTCAAAGCGGCTCAAA
AGCAACACTACTGCTTATGAGAATTACCACAGGATTTATGTTCCTGAGGG
GCAACCTCCGAAGTGTGAACCCAAAGAGCCCTTGAGAGTGAATGTTCTGT
TCCAACATATACAGAAGATGCTGTCTAGTGAAACTGCTGTGATTGCTGAG
ACAGGGGACTCTTGGTTTNNACTGCAGAAACTGAAATTGCCTAGGNATGC
GGGTTGAACTTCCAATGCATTGGGGATCATTGGGTGGGCTGTGGGGGCTA
TCCTTGATATTCCCGCTTTGTCCCAAAAAAAGGGCCTTTGGTTCAAAGGG
AGGGACCCTCCCGGAACTTGCCAGAAGGTTCCAAATGCTTGCAAGTGGGC
AAAAAAATTAACTCTCCTGTTAAAAGGGGGGGTCCCCCTTAAGTGGAACC
CCGGGGGGCCCCCAGGTGGTTTAAAAAGGGAAAACCCCCGGGGGTGGGGG
GCCCCCCACGGGGGAGAGGGTGGTGTAACACCAGGTTTTCTTTGGGGGAG
GTCCCACACACCTTTAAACACCCCCGCCCCCAAAGGGGTTGTGGCCTTTT
AAAAAGTGTGTCCCCGAGGAAAAACGCAAATTTTTTTTTGGGGGGTGCCT
GCCCTCTATCGTAAGCCGCCCCCCCAATTTGAAAAAAGGGGGGGGCGTTT
TTTAGTGCGCGCACAACAATATAAGAATCACGGCGGGACGGTGGTGGGTA
GGTCGGTGGAAGACGAAGAGACAAACAGAGTCGGCGCCCTGTTTGTTCTC
GTTTTTAAGAAAGAAGAAAGAGAGCGGACGCCCAAACCCATTCTGTGCAA
GCCGTGCGGCCTCCCACTTT
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