CN181614

Overview
NameCN181614
Unique NameCN181614
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length730
Libraries
Library NameType
Ruby Orange Developing Flower cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_CHICK (Eukaryotic translation initiation factor 2 subunit 1 OS=Gallus gallus GN=EIF2S1 PE=2 SV=2)

HSP 1 Score: 100.523 bits (249), Expect = 2.830e-23
Identity = 45/109 (41.28%), Postives = 75/109 (68.81%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +K+A+R        + P+KI L+APP YV+TT TL++ +G+SVLN+A+A   E IE+ +G   V+ EP+ V++ D+  LA  + +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLEREN 297

HSP 2 Score: 28.1054 bits (61), Expect = 2.830e-23
Identity = 12/22 (54.55%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L +TEE +  L+ NI RR+TPQ
Sbjct:  166 LDLTEEERRVLIDNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_XENLA (Eukaryotic translation initiation factor 2 subunit 1 OS=Xenopus laevis GN=eif2s1 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 6.234e-23
Identity = 45/109 (41.28%), Postives = 76/109 (69.72%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +KDA+R   +    + P+KI L+APP YV+TT TL++ +G+SVLN+A++   E IE+ +G   V+ EP+ V++ D+  LA  + +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKDALRAGLSCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMSVIKERIEEKRGVFNVQMEPKVVTDTDETELARQLERLEKEN 297

HSP 2 Score: 25.409 bits (54), Expect = 6.234e-23
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L ++E+ +  L+ NI RR+TPQ
Sbjct:  166 LDLSEDERRVLIDNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_XENTR (Eukaryotic translation initiation factor 2 subunit 1 OS=Xenopus tropicalis GN=eif2s1 PE=2 SV=1)

HSP 1 Score: 102.064 bits (253), Expect = 8.112e-23
Identity = 45/109 (41.28%), Postives = 76/109 (69.72%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +KDA+R   +    + P+KI L+APP YV+TT TL++ +G+SVLN+A++   E IE+ +G   V+ EP+ V++ D+  LA  + +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKDALRAGLSCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMSVIKERIEEKRGVFNVQMEPKVVTDTDETELARQLERLEKEN 297

HSP 2 Score: 25.0238 bits (53), Expect = 8.112e-23
Identity = 10/22 (45.45%), Postives = 15/22 (68.18%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L + E+ +  L+ NI RR+TPQ
Sbjct:  166 LDLNEDERRVLIDNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_RAT (Eukaryotic translation initiation factor 2 subunit 1 OS=Rattus norvegicus GN=Eif2s1 PE=1 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 1.055e-22
Identity = 45/109 (41.28%), Postives = 74/109 (67.89%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +K+A+R          P+KI L+APP YV+TT TL++ +G+SVLN+A+A   E IE+ +G   V+ EP+ V++ D+  LA  + +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKEALRAGLNCSTETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLEREN 297

HSP 2 Score: 26.9498 bits (58), Expect = 1.055e-22
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L + E+ ++ L+ NI RR+TPQ
Sbjct:  166 LDLNEDEREVLINNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_PONAB (Eukaryotic translation initiation factor 2 subunit 1 OS=Pongo abelii GN=EIF2S1 PE=2 SV=1)

HSP 1 Score: 99.7525 bits (247), Expect = 1.055e-22
Identity = 45/109 (41.28%), Postives = 75/109 (68.81%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +K+A+R        + P+KI L+APP YV+TT TL++ +G+SVL++A+A   E IE+ +G   V+ EP+ V++ D+  LA  M +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLEREN 297

HSP 2 Score: 26.9498 bits (58), Expect = 1.055e-22
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L + E+ ++ L+ NI RR+TPQ
Sbjct:  166 LDLNEDEREVLINNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_MOUSE (Eukaryotic translation initiation factor 2 subunit 1 OS=Mus musculus GN=Eif2s1 PE=1 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 1.055e-22
Identity = 45/109 (41.28%), Postives = 74/109 (67.89%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +K+A+R          P+KI L+APP YV+TT TL++ +G+SVLN+A+A   E IE+ +G   V+ EP+ V++ D+  LA  + +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKEALRAGLNCSTETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLEREN 297

HSP 2 Score: 26.9498 bits (58), Expect = 1.055e-22
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L + E+ ++ L+ NI RR+TPQ
Sbjct:  166 LDLNEDEREVLINNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_HUMAN (Eukaryotic translation initiation factor 2 subunit 1 OS=Homo sapiens GN=EIF2S1 PE=1 SV=3)

HSP 1 Score: 99.7525 bits (247), Expect = 1.055e-22
Identity = 45/109 (41.28%), Postives = 75/109 (68.81%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +K+A+R        + P+KI L+APP YV+TT TL++ +G+SVL++A+A   E IE+ +G   V+ EP+ V++ D+  LA  M +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKEALRAGLNCSTENMPIKINLIAPPRYVMTTTTLERTEGLSVLSQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQMERLEREN 297

HSP 2 Score: 26.9498 bits (58), Expect = 1.055e-22
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L + E+ ++ L+ NI RR+TPQ
Sbjct:  166 LDLNEDEREVLINNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_BOVIN (Eukaryotic translation initiation factor 2 subunit 1 OS=Bos taurus GN=EIF2S1 PE=2 SV=2)

HSP 1 Score: 99.7525 bits (247), Expect = 1.055e-22
Identity = 45/109 (41.28%), Postives = 74/109 (67.89%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRADIE+ C+ ++G+  +K+A+R          P+KI L+APP YV+TT TL++ +G+SVLN+A+A   E IE+ +G   V+ EP+ V++ D+  LA  + +L ++N
Sbjct:  189 VKIRADIEVACYGYEGIDAVKEALRAGLNCSTETMPIKINLIAPPRYVMTTTTLERTEGLSVLNQAMAVIKEKIEEKRGVFNVQMEPKVVTDTDETELARQLERLEREN 297

HSP 2 Score: 26.9498 bits (58), Expect = 1.055e-22
Identity = 10/22 (45.45%), Postives = 16/22 (72.73%), Query Frame = -2 
Query:  643 LAVTEEVKDALVKNIRRRMTPQ 708
            L + E+ ++ L+ NI RR+TPQ
Sbjct:  166 LDLNEDEREVLINNINRRLTPQ 187
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_SCHPO (Eukaryotic translation initiation factor 2 subunit alpha OS=Schizosaccharomyces pombe GN=tif211 PE=1 SV=1)

HSP 1 Score: 102.834 bits (255), Expect = 5.123e-22
Identity = 51/109 (46.79%), Postives = 72/109 (66.06%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRAD+E+ CF ++G+  IK A++ AE     + P+K+KLVAPPLYVL T  LDK  G+  L +AI A  ++I    G  TVK +P+AVSE D+  LA+ M K  ++N
Sbjct:  183 IKIRADVEVTCFGYEGINAIKAALKAAEDVHTEEVPIKVKLVAPPLYVLLTNALDKSLGLKKLEEAIGAIEKSITASNGTCTVKMKPKAVSETDELELADLMKKFEKEN 291

HSP 2 Score: 21.557 bits (44), Expect = 5.123e-22
Identity = 8/15 (53.33%), Postives = 11/15 (73.33%), Query Frame = -2 
Query:  634 LVKNIRRRMTPQPFE 678
            L+  I RR+TPQP +
Sbjct:  170 LLAQISRRLTPQPIK 184
[Close]
BLAST of CN181614 vs. ExPASy Swiss-Prot
Match: IF2A_YEAST (Eukaryotic translation initiation factor 2 subunit alpha OS=Saccharomyces cerevisiae GN=SUI2 PE=1 SV=1)

HSP 1 Score: 95.5153 bits (236), Expect = 3.076e-19
Identity = 49/109 (44.95%), Postives = 66/109 (60.55%), Query Frame = -3 
Query:  312 LKIRADIEMKCFQFDGVLHIKDAMRKAEAAGNNDCPVKIKLVAPPLYVLTTQTLDKEQGISVLNKAIAACTEAIEKHKGKLTVKEEPRAVSERDDKLLAEHMAKLHQDN 638
            +KIRAD+E+ CF ++G+  IKDA++ AE        VK+KLVA PLYVLTTQ LDK++GI  L  AI   TE I K+ G   +   P+AV+  +D  L   +     DN
Sbjct:  182 VKIRADVEVSCFSYEGIDAIKDALKSAEDMSTEQMQVKVKLVAAPLYVLTTQALDKQKGIEQLESAIEKITEVITKYGGVCNITMPPKAVTATEDAELQALLESKELDN 290
The following BLAST results are available for this feature:
BLAST of CN181614 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 12
Match NameE-valueIdentityDescription
IF2A_CHICK2.830e-2341.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_XENLA6.234e-2341.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_XENTR8.112e-2341.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_RAT1.055e-2241.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_PONAB1.055e-2241.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_MOUSE1.055e-2241.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_HUMAN1.055e-2241.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_BOVIN1.055e-2241.28Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_SCHPO5.123e-2246.79Eukaryotic translation initiation factor 2 subunit... [more]
IF2A_YEAST3.076e-1944.95Eukaryotic translation initiation factor 2 subunit... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS04_0001D11_f Ruby Orange Developing Flower cDNA Library Citrus sinensis cDNA clone CS_REb0001D11, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN181614 ID=CN181614; Name=CN181614; organism=Citrus sinensis; type=EST; length=730bp
CCTTTTTTTAGAGAACAGCTAGTTTTTCACATAATTATGAACTCAGTTGT
TGCCAACTATAATATATTCTTGTATTTTCCTTGCTCTAATCCGCCTCATG
AATTGCTAAACATGAAAAACAACTCCAACCATACAATGGTTTAAAAATTG
TCTTAAAGAACCAAAAAAAGAAAAAAAAATATTGGAAACAAAAAAATCAA
GCTACACTGCACTTTTCAATCCATAAACCCAGAACTTGAATTTTCCACAT
CAACTTCTCCCATTCCTGTGTCTTCCTCTTCTTCGCTATCTTCATCACCA
CTTACCTCCTCATTATCTTGATGTAGCTTAGCCATGTGCTCGGCGAGCAA
TTTGTCATCTCGTTCACTCACCGCTCTGGGCTCTTCCTTCACGGTAAGTT
TTCCCTTGTGCTTCTCTATAGCTTCAGTGCAAGCTGCAATTGCTTTATTG
AGAACTGAGATCCCTTGCTCCTTGTCAAGGGTCTGAGTAGTAAGCACATA
CAAGGGGGGAGCAACCAATTTGATTTTCACAGGGCAGTCATTGTTGCCAG
CAGCCTCAGCTTTCCGCATGGCATCCTTAATATGAAGAACTCCATCAAAC
TGGAAGCATTTCATTTCAATGTCAGCTCGAATCTTCAAATGGCTGTGGGG
TCATTCTTCTCCTAATATTTTTTACCAAAGCATCTTTCACTTCCTCCGTC
ACAGCAAGAACCACCTTAGTTACCTCCTGT
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