CN188048

Overview
NameCN188048
Unique NameCN188048
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length779
Libraries
Library NameType
Washington Navel Orange Stored Fruit Pulp cDNA Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
CN188048_ssr12 microsatellite CN188048_ssr12:12..55. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
BLAST of CN188048 vs. ExPASy Swiss-Prot
Match: DGK5_CAEEL (Putative diacylglycerol kinase K06A1.6 OS=Caenorhabditis elegans GN=dgk-5 PE=2 SV=2)

HSP 1 Score: 73.1738 bits (178), Expect = 1.832e-12
Identity = 59/194 (30.41%), Postives = 85/194 (43.81%), Query Frame = 1
Query:  187 KRSFAGNQTEMRDKKGFFNSVASGSGTILRPLHDLLHRSSEEAAATPKSKILNNYYI--PNYILVSGSEVQRSSLIPSCPVLVFINXXXXXXXXXXXXXTYRSLLNENQVIDLGEKAPDKVLHQLYVTLEKFKAAGDVFASEIEKRLRLIVAGGDGTASWLLGVVSDLKLPHSPPVATVPLGTGNNIPFSFGWG 762
            K  FA ++ E R  +G    +      ILR  +    RSS   +   K K  +  ++  P  +  SG         PS P+LVF+N             T   LLN  QV D+      K        LE F+        ++  +LR++V GGDGT  W+L  + +L  P  PP+A +PLGTGN++    GWG
Sbjct:  350 KNCFARDKLEERCNRGALKEMIVPPTWILRLANR--KRSSRTPSHPRKHKKSHRQFVVKPTDLWSSG---------PSQPLLVFVNPKSGGNKGSKALHTLCWLLNPRQVFDITSLKGPKF------GLEMFR--------KVVTQLRILVCGGDGTVGWVLSTLDNLNWPAYPPMAIMPLGTGNDLARCMGWG 518          
BLAST of CN188048 vs. ExPASy Swiss-Prot
Match: DGKA_DICDI (Diacylglycerol kinase A OS=Dictyostelium discoideum GN=dgkA PE=1 SV=3)

HSP 1 Score: 69.3218 bits (168), Expect = 2.645e-11
Identity = 47/159 (29.56%), Postives = 72/159 (45.28%), Query Frame = 1
Query:  289 LLHRSSEEAAATPKSKILNNYYIPNYILVSGSEVQRSSLIPSCPVLVFINXXXXXXXXXXXXXTYRSLLNENQVIDLGE-KAPDKVLHQLYVTLEKFKAAGDVFASEIEKRLRLIVAGGDGTASWLLGVVSDLKLPHSPPVATVPLGTGNNIPFSFGWG 762
            ++     + +++P S  LN     N   +   E+  +S +P   + VF+N                SLLN  Q+IDL +   PD  L  +   L K          E   R R++V GGDGT  WL   ++   +P + P+  +PLGTGN++  S GWG
Sbjct:  291 IIEEQQPQLSSSPSSPRLNINNNNNNNEIIEWELIENSNMPEKVLFVFVNSKSGGQFGSTLIRKLSSLLNPLQIIDLIKCGGPDSTLQMINRYLAKHP--------EQTNRFRILVCGGDGTVGWLFKQMTKHLVPSTIPIGIIPLGTGNDLARSLGWG 441          
BLAST of CN188048 vs. ExPASy Swiss-Prot
Match: DGKI_HUMAN (Diacylglycerol kinase iota OS=Homo sapiens GN=DGKI PE=1 SV=1)

HSP 1 Score: 68.9366 bits (167), Expect = 3.454e-11
Identity = 37/115 (32.17%), Postives = 55/115 (47.83%), Query Frame = 1
Query:  418 PVLVFINXXXXXXXXXXXXXTYRSLLNENQVIDLGEKAPDKVLHQLYVTLEKFKAAGDVFASEIEKRLRLIVAGGDGTASWLLGVVSDLKLPHSPPVATVPLGTGNNIPFSFGWG 762
            P+LVF+N              +   LN  QV DL ++ P   L +LY  +                 LR++  GGDGT  W+L ++ +L+L   PPV  +PLGTGN++  +  WG
Sbjct:  376 PLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDAL-ELYRKVPN---------------LRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWG 474          
BLAST of CN188048 vs. ExPASy Swiss-Prot
Match: DGKA_HUMAN (Diacylglycerol kinase alpha OS=Homo sapiens GN=DGKA PE=1 SV=3)

HSP 1 Score: 68.9366 bits (167), Expect = 3.454e-11
Identity = 43/131 (32.82%), Postives = 62/131 (47.33%), Query Frame = 1
Query:  373 VSGSEVQRSSLIPSC-PVLVFINXXXXXXXXXXXXXTYRSLLNENQVIDLGEKAPDKVLHQLYVTLEKFKAAGDVFASEIEKRLRLIVAGGDGTASWLLGVVSDLKLPHSPPVATVPLGTGNNIPFSFGWG 762
            +S SE  R   +P+  P+LVF+N              ++ +LN  QV +L +  P+       + L  FK   D          R++V GGDGT  W+L  +    LP  PPVA +PLGTGN++     WG
Sbjct:  360 LSTSEALRIDPVPNTHPLLVFVNPKSGGKQGQRVLWKFQYILNPRQVFNLLKDGPE-------IGLRLFKDVPDS---------RILVCGGDGTVGWILETIDKANLPVLPPVAVLPLGTGNDLARCLRWG 474          
BLAST of CN188048 vs. ExPASy Swiss-Prot
Match: DGKZ_HUMAN (Diacylglycerol kinase zeta OS=Homo sapiens GN=DGKZ PE=1 SV=3)

HSP 1 Score: 67.781 bits (164), Expect = 7.696e-11
Identity = 37/115 (32.17%), Postives = 55/115 (47.83%), Query Frame = 1
Query:  418 PVLVFINXXXXXXXXXXXXXTYRSLLNENQVIDLGEKAPDKVLHQLYVTLEKFKAAGDVFASEIEKRLRLIVAGGDGTASWLLGVVSDLKLPHSPPVATVPLGTGNNIPFSFGWG 762
            P+LVF+N             ++   LN  QV DL +  P + L ++Y  +                 LR++  GGDGT  W+L  +  L+L   PPVA +PLGTGN++  +  WG
Sbjct:  484 PLLVFVNPKSGGNQGAKIIQSFLWYLNPRQVFDLSQGGPKEAL-EMYRKVHN---------------LRILACGGDGTVGWILSTLDQLRLKPPPPVAILPLGTGNDLARTLNWG 582          
The following BLAST results are available for this feature:
BLAST of CN188048 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 5
Match NameE-valueIdentityDescription
DGK5_CAEEL1.832e-1230.41Putative diacylglycerol kinase K06A1.6 OS=Caenorha... [more]
DGKA_DICDI2.645e-1129.56Diacylglycerol kinase A OS=Dictyostelium discoideu... [more]
DGKI_HUMAN3.454e-1132.17Diacylglycerol kinase iota OS=Homo sapiens GN=DGKI... [more]
DGKA_HUMAN3.454e-1132.82Diacylglycerol kinase alpha OS=Homo sapiens GN=DGK... [more]
DGKZ_HUMAN7.696e-1132.17Diacylglycerol kinase zeta OS=Homo sapiens GN=DGKZ... [more]
back to top
Properties
Property NameValue
Genbank descriptionUCRCS05_0005G18_r Washington Navel Orange Stored Fruit Pulp cDNA Library Citrus sinensis cDNA clone CS_WEb0005G18, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CN188048 ID=CN188048; Name=CN188048; organism=Citrus sinensis; type=EST; length=779bp
GAGGCTGAAACATATATATATATATATATATATATATATATATATATATA
TATATGTATATGAGAAATTACCTAACTCAAACCAGAAAGTACATAACGAA
ACAACAGACCAGAGTAGTTGATTTAAGACGAAACAATAAGAATAAGAGCA
AACGTCACGATAACATGATTCATGAAAAACAACAACAAAAGAAGCTTCGC
AGGTAACCAAACAGAGATGAGAGACAAAAAGGGATTCTTTAATAGCGTTG
CCTCTGGTTCTGGGACTATCTTGCGTCCCTTACATGATCTCCTACACAGA
AGTTCAGAGGAAGCGGCGGCTACTCCCAAATCAAAGATTTTAAACAACTA
TTACATTCCAAATTACATTCTTGTATCTGGATCGGAGGTTCAAAGATCTA
GCCTCATACCATCTTGTCCGGTGCTAGTGTTCATCAACTCAAAAAGTGGC
GGCCAGTTGGGCGGCAAGCTTCTGCTCACTTACCGCTCCCTTCTTAATGA
AAACCAGGTTATTGACTTGGGAGAAAAGGCACCTGATAAAGTGTTACATC
AGCTTTATGTCACTTTAGAGAAGTTCAAGGCTGCAGGAGATGTTTTTGCT
TCTGAAATTGAGAAGAGGTTGAGATTAATTGTTGCAGGCGGAGATGGTAC
GGCTAGCTGGCTCCTTGGAGTTGTTAGTGATCTAAAACTACCTCATTCAC
CTCCAGTTGCGACAGTGCCACTAGGAACAGGAAACAACATCCCGTTCTCA
TTTGGGTGGGGAAAAGAAAAATCCAAACA
back to top