CX078338

Overview
NameCX078338
Unique NameCX078338
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length500
Libraries
Library NameType
Parent Washington Navel Orange Callus cDNA Library UCRCS08-2cdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: RH35_ARATH (DEAD-box ATP-dependent RNA helicase 35 OS=Arabidopsis thaliana GN=RH35 PE=2 SV=1)

HSP 1 Score: 251.521 bits (641), Expect = 1.558e-66
Identity = 118/127 (92.91%), Postives = 124/127 (97.64%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 383
            DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPME+ + I NASGVKGCAYCGGLGHRIRDCPKLEHQKS+AI++SR+DYFGSGGYRGEI
Sbjct:  465 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEEAETIANASGVKGCAYCGGLGHRIRDCPKLEHQKSVAISNSRKDYFGSGGYRGEI 591          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: RH35A_ORYSJ (DEAD-box ATP-dependent RNA helicase 35A OS=Oryza sativa subsp. japonica GN=Os02g0150100 PE=2 SV=2)

HSP 1 Score: 241.121 bits (614), Expect = 2.106e-63
Identity = 112/127 (88.19%), Postives = 119/127 (93.70%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 383
            DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQ+ETTLLDLKHLL+EAKQRIPPVLAELNDP+ED + +   SGVKGCAYCGGLGHR+ DCPKLEHQKSMAIA SRRDY+G GGYRGEI
Sbjct:  501 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQTETTLLDLKHLLKEAKQRIPPVLAELNDPLEDEETMAKESGVKGCAYCGGLGHRVTDCPKLEHQKSMAIAGSRRDYYGGGGYRGEI 627          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: RH43_ARATH (Putative DEAD-box ATP-dependent RNA helicase 43 OS=Arabidopsis thaliana GN=RH43 PE=2 SV=1)

HSP 1 Score: 234.572 bits (597), Expect = 1.971e-61
Identity = 110/127 (86.61%), Postives = 117/127 (92.13%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 383
            DIQHVINYDMP EIENYVHRIGRTGRCGKTGIATTFINKNQSE TLLDLKHLLQEAKQRIPPVLAELN PME+ + I NASGVKGCAYCGGLGHRI  CPK EHQKS+AI+SSR+D+FGS GYRGE+
Sbjct:  416 DIQHVINYDMPGEIENYVHRIGRTGRCGKTGIATTFINKNQSEITLLDLKHLLQEAKQRIPPVLAELNGPMEETETIANASGVKGCAYCGGLGHRILQCPKFEHQKSVAISSSRKDHFGSDGYRGEV 542          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: RH35B_ORYSJ (DEAD-box ATP-dependent RNA helicase 35B OS=Oryza sativa subsp. japonica GN=Os06g0697200 PE=3 SV=1)

HSP 1 Score: 201.83 bits (512), Expect = 1.416e-51
Identity = 97/128 (75.78%), Postives = 109/128 (85.16%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVD-AITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGGYRGEI 383
            DIQHVINYDMPAEIENYVHRIGRTGR GKTG+ATTFINKNQ+ETTLLDLK LL E+KQR+PP+LA+L+DP ED   AI   SGVKGCA+CGGLGHRI  CPK + Q S+ +A +R DYFG GGYRGEI
Sbjct:  492 DIQHVINYDMPAEIENYVHRIGRTGRRGKTGVATTFINKNQTETTLLDLKQLLIESKQRLPPILADLDDPQEDDKVAIAQQSGVKGCAFCGGLGHRIEACPKQQLQNSVTLARARSDYFGGGGYRGEI 619          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: DDX41_HUMAN (Probable ATP-dependent RNA helicase DDX41 OS=Homo sapiens GN=DDX41 PE=1 SV=2)

HSP 1 Score: 145.206 bits (365), Expect = 1.573e-34
Identity = 73/117 (62.39%), Postives = 89/117 (76.07%), Query Frame = 3
Query:    6 IQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIAS-SRRDY 353
            IQHVINYDMP EIENYVHRIGRTGR G TGIATTFINK   E+ L+DLK LL EAKQ++PPVL  L+   E   ++ +  G +GCA+CGGLGHRI DCPKLE  ++  +++  R+DY
Sbjct:  501 IQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVPPVLQVLHCGDE---SMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDY 614          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: DDX41_DICDI (Probable ATP-dependent RNA helicase ddx41 OS=Dictyostelium discoideum GN=ddx41 PE=1 SV=1)

HSP 1 Score: 143.28 bits (360), Expect = 5.977e-34
Identity = 71/132 (53.79%), Postives = 91/132 (68.94%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASG----------VKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGG 368
            +IQHVIN+DMP EIENY+HRIGRTGR G  G+ATTFINKN +E+ LLDLK+LL EAKQ++PP L E+ D  + +  + + +G           K C YC G GHR+ +CPKL+ Q     A  +RD+FGSGG
Sbjct:  542 EIQHVINFDMPREIENYIHRIGRTGRRGNKGVATTFINKNNTESLLLDLKYLLIEAKQKVPPALLEIPDDNQYLQKLQDRNGNTGGGADDDDTKPCEYCDGRGHRLVNCPKLKKQ-----AGPKRDFFGSGG 668          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: DDX41_MOUSE (Probable ATP-dependent RNA helicase DDX41 OS=Mus musculus GN=Ddx41 PE=1 SV=1)

HSP 1 Score: 142.51 bits (358), Expect = 1.019e-33
Identity = 72/117 (61.54%), Postives = 88/117 (75.21%), Query Frame = 3
Query:    6 IQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIAS-SRRDY 353
            IQHVINYDMP EIENYVHRIGRTGR G TGIATTFINK   E+ L+DLK LL EAKQ++PP L  L+   E   ++ +  G +GCA+CGGLGHRI DCPKLE  ++  +++  R+DY
Sbjct:  501 IQHVINYDMPEEIENYVHRIGRTGRSGNTGIATTFINKACDESVLMDLKALLLEAKQKVPPGLQVLHCGDE---SMLDIGGERGCAFCGGLGHRITDCPKLEAMQTKQVSNIGRKDY 614          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: DDX41_DROME (ATP-dependent RNA helicase abstrakt OS=Drosophila melanogaster GN=abs PE=1 SV=1)

HSP 1 Score: 129.798 bits (325), Expect = 6.838e-30
Identity = 68/121 (56.20%), Postives = 77/121 (63.64%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEH-QKSMAIASSRRDYFGS 362
            ++QHVINYDMP +IENYVHRIGRTGR    G+ATT INK   ++ LLDLKHLL E KQ +P  L EL    E        S   GC YCGGLGHRI +CPKLE  Q   A    RRDY  +
Sbjct:  496 NVQHVINYDMPDDIENYVHRIGRTGRSNTKGLATTLINKTTEQSVLLDLKHLLIEGKQEVPDFLDELAPETEHQHLDLGDS--HGCTYCGGLGHRITECPKLEAVQNKQASNIGRRDYLSN 614          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: DDX3X_MOUSE (ATP-dependent RNA helicase DDX3X OS=Mus musculus GN=Ddx3x PE=1 SV=3)

HSP 1 Score: 79.337 bits (194), Expect = 1.060e-14
Identity = 50/122 (40.98%), Postives = 67/122 (54.92%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVLAELNDPMEDVDAITNASGVKGCAYCGGLGHRIRDCPKLEHQKSMAIASSRRDYFGSGG 368
            +++HVIN+D+P++IE YVHRIGRTGR G  G+AT+F N+     T  DL  LL EAKQ +P  L   N   E     ++    K   + GG G   RD  +     S + +SSR     SGG
Sbjct:  509 NVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINIT-KDLLDLLVEAKQEVPSWLE--NMAFEHHYKGSSRGRSKSSRFSGGFG--ARDYRQSSGASSSSFSSSRASSSRSGG 625          
BLAST of CX078338 vs. ExPASy Swiss-Prot
Match: DED1_MAGGR (ATP-dependent RNA helicase DED1 OS=Magnaporthe grisea GN=DED1 PE=3 SV=2)

HSP 1 Score: 78.9518 bits (193), Expect = 1.384e-14
Identity = 35/64 (54.69%), Postives = 47/64 (73.44%), Query Frame = 3
Query:    3 DIQHVINYDMPAEIENYVHRIGRTGRCGKTGIATTFINKNQSETTLLDLKHLLQEAKQRIPPVL 194
            ++ HVINYD+P +I++YVHRIGRTGR G TGIAT F +   +     +L ++L+EAKQ IPP L
Sbjct:  517 NVTHVINYDLPTDIDDYVHRIGRTGRAGNTGIATAFFDMKDNSGVAQELLNILKEAKQDIPPFL 580          
The following BLAST results are available for this feature:
BLAST of CX078338 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 94
Match NameE-valueIdentityDescription
RH35_ARATH1.558e-6692.91DEAD-box ATP-dependent RNA helicase 35 OS=Arabidop... [more]
RH35A_ORYSJ2.106e-6388.19DEAD-box ATP-dependent RNA helicase 35A OS=Oryza s... [more]
RH43_ARATH1.971e-6186.61Putative DEAD-box ATP-dependent RNA helicase 43 OS... [more]
RH35B_ORYSJ1.416e-5175.78DEAD-box ATP-dependent RNA helicase 35B OS=Oryza s... [more]
DDX41_HUMAN1.573e-3462.39Probable ATP-dependent RNA helicase DDX41 OS=Homo ... [more]
DDX41_DICDI5.977e-3453.79Probable ATP-dependent RNA helicase ddx41 OS=Dicty... [more]
DDX41_MOUSE1.019e-3361.54Probable ATP-dependent RNA helicase DDX41 OS=Mus m... [more]
DDX41_DROME6.838e-3056.20ATP-dependent RNA helicase abstrakt OS=Drosophila ... [more]
DDX3X_MOUSE1.060e-1440.98ATP-dependent RNA helicase DDX3X OS=Mus musculus G... [more]
DED1_MAGGR1.384e-1454.69ATP-dependent RNA helicase DED1 OS=Magnaporthe gri... [more]

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Properties
Property NameValue
Genbank descriptionUCRCS08_8E03_b Parent Washington Navel Orange Callus cDNA Library UCRCS08-2 Citrus sinensis cDNA clone UCRCS08-8E03-J6-1-5.b, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>CX078338 ID=CX078338; Name=CX078338; organism=Citrus sinensis; type=EST; length=500bp
CCGATATTCAGCATGTCATTAATTATGACATGCCTGCAGAAATAGAGAAC
TATGTCCACAGAATTGGACGAACGGGAAGATGTGGGAAGACTGGAATTGC
AACAACATTTATAAACAAGAACCAAAGTGAGACGACACTCCTTGATTTGA
AGCACCTGCTGCAAGAAGCAAAACAGAGGATTCCTCCTGTGCTGGCTGAG
CTAAATGATCCGATGGAGGATGTGGATGCAATTACAAATGCAAGTGGGGT
AAAGGGATGTGCATACTGTGGTGGGCTTGGTCATCGTATCCGTGATTGTC
CCAAGTTAGAGCATCAGAAGAGCATGGCGATTGCCAGCTCTAGAAGGGAT
TATTTTGGTTCTGGAGGTTATAGAGGAGAAATTTGATGTACACTTATCAC
GGATGTATCTTCTTAAATTGATAGCTATTCTGGTAATGAATTCATCTGTA
CTTTATTTTTCTGTTTTTTTTAATAAAGAATGAAATTTCCAAAAAAAAAA
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