EL492680

Overview
NameEL492680
Unique NameEL492680
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length179
Libraries
Library NameType
Citrus sinensis flesh cDNA-AFLP Librarycdna_library
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
Csv1_Contig12932 contig Csv1_Contig12932:188..366. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA2_ARATH (Phospholipase D alpha 2 OS=Arabidopsis thaliana GN=PLDALPHA2 PE=2 SV=1)

HSP 1 Score: 91.6633 bits (226), Expect = 5.671e-21
Identity = 39/45 (86.67%), Postives = 41/45 (91.11%), Query Frame = 3 
Query:   45 GYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            GYQPYHLSTR PARGQIHGFRMSLWYEHLGMLD+TFL P S EC+
Sbjct:  696 GYQPYHLSTRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSQECI 740

HSP 2 Score: 28.4906 bits (62), Expect = 5.671e-21
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  682 RSMDGARDSEIAMG 695
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_ORYSJ (Phospholipase D alpha 1 OS=Oryza sativa subsp. japonica GN=PLD1 PE=1 SV=2)

HSP 1 Score: 88.5817 bits (218), Expect = 4.645e-20
Identity = 38/45 (84.44%), Postives = 41/45 (91.11%), Query Frame = 3 
Query:   45 GYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            GYQPYHL+TR PARGQIHGFRM+LWYEHLGMLDD F +PES ECV
Sbjct:  698 GYQPYHLATRQPARGQIHGFRMALWYEHLGMLDDVFQRPESLECV 742

HSP 2 Score: 28.4906 bits (62), Expect = 4.645e-20
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  684 RSMDGARDSEIAMG 697
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_RICCO (Phospholipase D alpha 1 OS=Ricinus communis GN=PLD1 PE=1 SV=1)

HSP 1 Score: 88.1965 bits (217), Expect = 6.041e-20
Identity = 38/44 (86.36%), Postives = 41/44 (93.18%), Query Frame = 3 
Query:   48 YQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            YQP+HLSTR PARGQIHGFRMSLWYEHLGMLD++FL PES ECV
Sbjct:  695 YQPHHLSTRQPARGQIHGFRMSLWYEHLGMLDESFLNPESEECV 738

HSP 2 Score: 28.4906 bits (62), Expect = 6.041e-20
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  680 RSMDGARDSEIAMG 693
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_TOBAC (Phospholipase D alpha 1 OS=Nicotiana tabacum GN=PLD1 PE=2 SV=2)

HSP 1 Score: 87.4261 bits (215), Expect = 1.022e-19
Identity = 37/44 (84.09%), Postives = 41/44 (93.18%), Query Frame = 3 
Query:   48 YQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            YQP+HL+TR PARGQIHGFRM+LWYEHLGMLD+TFL PES ECV
Sbjct:  695 YQPHHLATREPARGQIHGFRMALWYEHLGMLDETFLHPESEECV 738

HSP 2 Score: 28.4906 bits (62), Expect = 1.022e-19
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  680 RSMDGARDSEIAMG 693
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_BRAOC (Phospholipase D alpha 1 OS=Brassica oleracea var. capitata GN=PLD1 PE=2 SV=1)

HSP 1 Score: 86.2705 bits (212), Expect = 2.247e-19
Identity = 37/45 (82.22%), Postives = 40/45 (88.89%), Query Frame = 3 
Query:   45 GYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            GYQP+HLS R PARGQIHGFRMSLWYEHLGMLD+TFL P S EC+
Sbjct:  696 GYQPHHLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSVECI 740

HSP 2 Score: 28.4906 bits (62), Expect = 2.247e-19
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  682 RSMDGARDSEIAMG 695
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_ARATH (Phospholipase D alpha 1 OS=Arabidopsis thaliana GN=PLDALPHA1 PE=1 SV=2)

HSP 1 Score: 86.2705 bits (212), Expect = 2.247e-19
Identity = 37/45 (82.22%), Postives = 40/45 (88.89%), Query Frame = 3 
Query:   45 GYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            GYQP+HLS R PARGQIHGFRMSLWYEHLGMLD+TFL P S EC+
Sbjct:  696 GYQPHHLSHRQPARGQIHGFRMSLWYEHLGMLDETFLDPSSLECI 740

HSP 2 Score: 28.4906 bits (62), Expect = 2.247e-19
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  682 RSMDGARDSEIAMG 695
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA2_BRAOC (Phospholipase D alpha 2 OS=Brassica oleracea var. capitata GN=PLD2 PE=1 SV=2)

HSP 1 Score: 85.8853 bits (211), Expect = 2.922e-19
Identity = 36/45 (80.00%), Postives = 40/45 (88.89%), Query Frame = 3 
Query:   45 GYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            GYQP+HLS R PARGQ+HGFRMSLWYEHLGMLD+TFL P S EC+
Sbjct:  698 GYQPHHLSHRQPARGQVHGFRMSLWYEHLGMLDETFLDPSSLECI 742

HSP 2 Score: 28.4906 bits (62), Expect = 2.922e-19
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  684 RSMDGARDSEIAMG 697
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_MAIZE (Phospholipase D alpha 1 OS=Zea mays GN=PLD1 PE=2 SV=1)

HSP 1 Score: 85.8853 bits (211), Expect = 2.922e-19
Identity = 37/44 (84.09%), Postives = 40/44 (90.91%), Query Frame = 3 
Query:   48 YQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            YQPYHL+TR PARGQIHGFRMSLWYEHLGML+D F +PES ECV
Sbjct:  699 YQPYHLATRQPARGQIHGFRMSLWYEHLGMLEDVFQRPESVECV 742

HSP 2 Score: 28.4906 bits (62), Expect = 2.922e-19
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  684 RSMDGARDSEIAMG 697
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_VIGUN (Phospholipase D alpha 1 OS=Vigna unguiculata GN=PLD1 PE=2 SV=1)

HSP 1 Score: 82.4185 bits (202), Expect = 3.105e-18
Identity = 34/45 (75.56%), Postives = 39/45 (86.67%), Query Frame = 3 
Query:   45 GYQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            GYQPYHL+   PARGQ++GFRMSLWYEHLGML DTF +PES EC+
Sbjct:  695 GYQPYHLANTQPARGQVYGFRMSLWYEHLGMLHDTFQRPESEECI 739

HSP 2 Score: 28.4906 bits (62), Expect = 3.105e-18
Identity = 12/14 (85.71%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GARD+EIAMG
Sbjct:  681 RSMDGARDSEIAMG 694
[Close]
BLAST of EL492680 vs. ExPASy Swiss-Prot
Match: PLDA1_PIMBR (Phospholipase D alpha 1 OS=Pimpinella brachycarpa GN=PLD1 PE=2 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 4.038e-18
Identity = 36/44 (81.82%), Postives = 39/44 (88.64%), Query Frame = 3 
Query:   48 YQPYHLSTRVPARGQIHGFRMSLWYEHLGMLDDTFLQPESSECV 179
            YQP+HL+TR PARGQIHGFRMSLWYEHLGMLDDT   PES +CV
Sbjct:  695 YQPHHLATREPARGQIHGFRMSLWYEHLGMLDDTLALPESVDCV 738

HSP 2 Score: 27.335 bits (59), Expect = 4.038e-18
Identity = 11/14 (78.57%), Postives = 14/14 (100.00%), Query Frame = 2 
Query:    2 RSMEGARDTEIAMG 43
            RSM+GA+D+EIAMG
Sbjct:  680 RSMDGAKDSEIAMG 693
The following BLAST results are available for this feature:
BLAST of EL492680 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 13
Match NameE-valueIdentityDescription
PLDA2_ARATH5.671e-2186.67Phospholipase D alpha 2 OS=Arabidopsis thaliana GN... [more]
PLDA1_ORYSJ4.645e-2084.44Phospholipase D alpha 1 OS=Oryza sativa subsp. jap... [more]
PLDA1_RICCO6.041e-2086.36Phospholipase D alpha 1 OS=Ricinus communis GN=PLD... [more]
PLDA1_TOBAC1.022e-1984.09Phospholipase D alpha 1 OS=Nicotiana tabacum GN=PL... [more]
PLDA1_BRAOC2.247e-1982.22Phospholipase D alpha 1 OS=Brassica oleracea var. ... [more]
PLDA1_ARATH2.247e-1982.22Phospholipase D alpha 1 OS=Arabidopsis thaliana GN... [more]
PLDA2_BRAOC2.922e-1980.00Phospholipase D alpha 2 OS=Brassica oleracea var. ... [more]
PLDA1_MAIZE2.922e-1984.09Phospholipase D alpha 1 OS=Zea mays GN=PLD1 PE=2 S... [more]
PLDA1_VIGUN3.105e-1875.56Phospholipase D alpha 1 OS=Vigna unguiculata GN=PL... [more]
PLDA1_PIMBR4.038e-1881.82Phospholipase D alpha 1 OS=Pimpinella brachycarpa ... [more]

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Properties
Property NameValue
Genbank descriptionC5450 Citrus sinensis flesh cDNA-AFLP Library Citrus sinensis cDNA clone ClC5450, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EL492680 ID=EL492680; Name=EL492680; organism=Citrus sinensis; type=EST; length=179bp
GCGATCAATGGAGGGTGCTAGGGACACTGAGATAGCCATGGGTAGGCTAC
CAACCATATCATTTGTCAACCAGGGTGCCAGCACGAGGCCAGATACACGG
CTTCCGTATGTCACTATGGTACGAGCACCTAGGCATGCTCGATGACACCT
TCCTCCAGCCAGAAAGTAGCGAATGTGTT
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