EY665835 | Citrus Genome Database

Overview


Name	EY665835
Unique Name	EY665835
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	874

Libraries


Library Name	Type
Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2)	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
EY665835_ssr27	microsatellite	EY665835_ssr27:27..41.	BLAST: Citrus ESTs to Prunus persica proteins V1

Homology

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BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CYSP_PEA (Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1)

HSP 1 Score: 228.794 bits (582), Expect = 3.139e-59
Identity = 124/219 (56.62%), Postives = 146/219 (66.67%), Query Frame = 3

Query:   75 NDDDAMIRQVVPSDGEQSEDHLLNAEHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQFLGLNRRLRLPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGGSALLIDGR--ACQPQGATACGLVITSGDSKGNLGHVTLGAMVGLWTLPFDTYSRVGGVNEKKGYPSPGTKG 725
            N+DD +IRQVV ++    EDHLLNAEHHF+ FKSKFSK+YAT+EEHDYRF VFK+NL +AK  Q  DPTA HG+TKFSDLT SEFRRQFLGL +RLRLPA AQKAPILPT +LP DFDWR+ GAVT VKDQG+CGSCW+FS T    G+  L  G+  +   Q    C  V     +    G    G   GL    F+     GGV ++K Y   G  G
Sbjct:   24 NNDDFIIRQVVDNE----EDHLLNAEHHFTSFKSKFSKSYATKEEHDYRFGVFKSNLIKAKLHQNRDPTAEHGITKFSDLTASEFRRQFLGLKKRLRLPAHAQKAPILPTTNLPEDFDWREKGAVTPVKDQGSCGSCWAFSTTGALEGAHYLATGKLVSLSEQQLVDCDHVCDPEQA----GSCDSGCNGGLMNNAFEYLLESGGVVQEKDYAYTGRDG 234

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: RD19A_ARATH (Cysteine proteinase RD19a OS=Arabidopsis thaliana GN=RD19A PE=2 SV=1)

HSP 1 Score: 210.305 bits (534), Expect = 1.155e-53
Identity = 123/248 (49.60%), Postives = 150/248 (60.48%), Query Frame = 3

Query:   81 DDAMIRQVVPSDGEQSEDHLLNAEHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQFLGLNRRLRLPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGGSALLIDGR--------------ACQPQGATACGLVITSGDSKGNLGHVTLGAMVGLWTLPFDTYSRVGGVNEKKGYPSPGTKGGFLAILTKNQNWVALGSNF 782
            DD +IRQVV      +E  +L +E HFSLFK KF K YA+ EEHDYRF VFKANLRRA+R Q LDP+A HGVT+FSDLT SEFR++ LG+    +LP DA KAPILPT +LP DFDWRDHGAVT VK+QG+CGSCWSFSAT    G+  L  G+               C P+ A +C       DS         G   GL    F+   + GG+ +++ YP  G K G    L K++  VA  SNF
Sbjct:   29 DDLVIRQVVGG----AEPQVLTSEDHFSLFKRKFGKVYASNEEHDYRFSVFKANLRRARRHQKLDPSATHGVTQFSDLTRSEFRKKHLGVRSGFKLPKDANKAPILPTENLPEDFDWRDHGAVTPVKNQGSCGSCWSFSATGALEGANFLATGKLVSLSEQQLVDCDHECDPEEADSC-------DS---------GCNGGLMNSAFEYTLKTGGLMKEEDYPYTG-KDGKTCKLDKSK-IVASVSNF 254

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: A494_ARATH (Probable cysteine proteinase A494 OS=Arabidopsis thaliana GN=At2g21430 PE=2 SV=2)

HSP 1 Score: 199.134 bits (505), Expect = 2.663e-50
Identity = 113/240 (47.08%), Postives = 143/240 (59.58%), Query Frame = 3

Query:   78 DDDAMIRQVVPSDGEQSEDHLLNAEHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQFLGLNRRLRLPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGGSALLIDGR--ACQPQGATACGLVITSGDSKGNLGHVTLGAMVGLWTLPFDTYSRVGGVNEKKGYPSPGTKGGFLAILTKNQNWVALGSNFQGV 791
            D+D +IRQVV    +++E  +L++E HF+LFK KF K Y + EEH YRF VFKANL RA R Q +DP+A HGVT+FSDLT SEFRR+ LG+    +LP DA +APILPT +LP +FDWRD GAVT VK+QG+CGSCWSFS T    G+  L  G+  +   Q    C       +     G    G   GL    F+   + GG+  +K YP  GT GG   +       VA  SNF  V
Sbjct:   25 DEDVLIRQVV----DETEPKVLSSEDHFTLFKKKFGKVYGSIEEHYYRFSVFKANLLRAMRHQKMDPSARHGVTQFSDLTRSEFRRKHLGVKGGFKLPKDANQAPILPTQNLPEEFDWRDRGAVTPVKNQGSCGSCWSFSTTGALEGAHFLATGKLVSLSEQQLVDCDHECDPEEE----GSCDSGCNGGLMNSAFEYTLKTGGLMREKDYPYTGTDGGSCKL--DRSKIVASVSNFSVV 254

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CYSP1_MAIZE (Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=1)

HSP 1 Score: 191.045 bits (484), Expect = 7.252e-48
Identity = 107/222 (48.20%), Postives = 130/222 (58.56%), Query Frame = 3

Query:   81 DDAMIRQVVPSDGEQSEDHLLNAEHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQFLGLNRRLR-----LPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGGSALLIDGR--ACQPQGATACGLVITSGDSKGNLGHVTLGAMVGLWTLPFDTYSRVGGVNEKKGYPSPGTKG 725
            +D +IRQVVP  G    D  LNAE HF  F  +F K+Y   +EH YR  VFK NLRRA+R QLLDP+A HGVTKFSDLTP+EFRR +LGL +  R     L   A +AP+LPT+ LP DFDWRDHGAV  VK+QG+CGSCWSFSA+    G+  L  G+      Q    C     S +          G   GL T  F    + GG+  +K YP  G+ G
Sbjct:   24 EDPLIRQVVP--GGDDNDLELNAESHFLSFVQRFGKSYKDADEHAYRLSVFKDNLRRARRHQLLDPSAEHGVTKFSDLTPAEFRRTYLGLRKSRRALLRELGESAHEAPVLPTDGLPDDFDWRDHGAVGPVKNQGSCGSCWSFSASGALEGAHYLATGKLEVLSEQQFVDCDHECDSSEPDS----CDSGCNGGLMTTAFSYLQKAGGLESEKDYPYTGSDG 239

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CYSP_TRYBB (Cysteine proteinase OS=Trypanosoma brucei brucei PE=1 SV=1)

HSP 1 Score: 95.9005 bits (237), Expect = 1.988e-24
Identity = 53/130 (40.77%), Postives = 68/130 (52.31%), Query Frame = 3

Query:  150 EHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQ-------FLGLNRRLRLPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGG 518
            E  F+ FK K+ K Y   +E  +RFR F+ N+ +AK +   +P A  GVT FSD+T  EFR +       F    +RLR      K   + T   P   DWR+ GAVT VK QG CGSCW+FS      G
Sbjct:   38 EMRFAAFKKKYGKVYKDAKEEAFRFRAFEENMEQAKIQAAANPYATFGVTPFSDMTREEFRARYRNGASYFAAAQKRLR------KTVNVTTGRAPAAVDWREKGAVTPVKVQGQCGSCWAFSTIGNIEG 161

HSP 2 Score: 36.965 bits (84), Expect = 1.988e-24
Identity = 18/43 (41.86%), Postives = 26/43 (60.47%), Query Frame = 2

Query:  620 DSGCNGGVMDSAF*YLLQGGGGERKKGIPFPWDQRGVPGNFDQ 748
            DSGCNGG+MD+AF +++   GG       +P+    V GN +Q
Sbjct:  185 DSGCNGGLMDNAFNWIVNSNGGNVFTEASYPY----VSGNGEQ 223

HSP 3 Score: 21.1718 bits (43), Expect = 1.988e-24
Identity = 11/23 (47.83%), Postives = 14/23 (60.87%), Query Frame = 1

Query:  505 GALEGAHFLSTGELVSLREQQLV 573
            G +EG   ++   LVSL EQ LV
Sbjct:  157 GNIEGQWQVAGNPLVSLSEQMLV 179

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CPR1_DROME (Putative cysteine proteinase CG12163 OS=Drosophila melanogaster GN=CG12163 PE=2 SV=2)

HSP 1 Score: 91.2781 bits (225), Expect = 1.637e-22
Identity = 47/126 (37.30%), Postives = 74/126 (58.73%), Query Frame = 3

Query:  150 EHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDP-TAVHGVTKFSDLTPSEFRRQFLGLNRRLRLPADAQKAPILPT--NDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGG 518
            +H F  F+ +F + Y +  E   R R+F+ NL+  +     +  +A +G+T+F+D+T SE++ +  GL +R    A    A ++P    +LP +FDWR   AVT VK+QG+CGSCW+FS T    G
Sbjct:  305 DHLFYKFQVRFGRRYVSTAERQMRLRIFRQNLKTIEELNANEMGSAKYGITEFADMTSSEYKER-TGLWQRDEAKATGGSAAVVPAYHGELPKEFDWRQKDAVTQVKNQGSCGSCWAFSVTGNIEG 429

HSP 2 Score: 29.6462 bits (65), Expect = 1.637e-22
Identity = 13/30 (43.33%), Postives = 19/30 (63.33%), Query Frame = 2

Query:  620 DSGCNGGVMDSAF*YLLQGGGGERKKGIPF 709
            DS CNGG+MD+A+  +   GG E +   P+
Sbjct:  453 DSACNGGLMDNAYKAIKDIGGLEYEAEYPY 482

HSP 3 Score: 26.5646 bits (57), Expect = 1.637e-22
Identity = 11/24 (45.83%), Postives = 16/24 (66.67%), Query Frame = 1

Query:  505 GALEGAHFLSTGELVSLREQQLVD 576
            G +EG + + TGEL    EQ+L+D
Sbjct:  425 GNIEGLYAVKTGELKEFSEQELLD 448

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: LMCPB_LEIME (Cysteine proteinase B OS=Leishmania mexicana GN=LMCPB PE=2 SV=2)

HSP 1 Score: 85.1149 bits (209), Expect = 8.137e-21
Identity = 47/124 (37.90%), Postives = 62/124 (50.00%), Query Frame = 3

Query:  159 FSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQFLG----LNRRLRLPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGG 518
            F  FK  + + Y T  E   R   F+ NL   +  Q  +P A  G+TKF DL+ +EF  ++L          R  A   +      + +P   DWR+ GAVT VKDQGACGSCW+FSA     G
Sbjct:   38 FEEFKRTYGRAYETLAEEQQRLANFERNLELMREHQARNPHAQFGITKFFDLSEAEFAARYLNGAAYFAAAKRHAAQHYRKARADLSAVPDAVDWREKGAVTPVKDQGACGSCWAFSAVGNIEG 161

HSP 2 Score: 28.4906 bits (62), Expect = 8.137e-21
Identity = 15/37 (40.54%), Postives = 20/37 (54.05%), Query Frame = 2

Query:  614 SCDS---GCNGGVMDSAF*YLLQGGGGERKKGIPFPW 715
            SCD    GC+GG+M  AF +LLQ   G       +P+
Sbjct:  180 SCDDMNDGCDGGLMLQAFDWLLQNTNGHLHTEDSYPY 216

HSP 3 Score: 28.1054 bits (61), Expect = 8.137e-21
Identity = 14/23 (60.87%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  505 GALEGAHFLSTGELVSLREQQLV 573
            G +EG  +L+  ELVSL EQQLV
Sbjct:  157 GNIEGQWYLAGHELVSLSEQQLV 179

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CYSP2_LEIPI (Cysteine proteinase 2 OS=Leishmania pifanoi GN=CYS2 PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 8.137e-21
Identity = 47/124 (37.90%), Postives = 62/124 (50.00%), Query Frame = 3

Query:  159 FSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDPTAVHGVTKFSDLTPSEFRRQFLG----LNRRLRLPADAQKAPILPTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGG 518
            F  FK  + + Y T  E   R   F+ NL   +  Q  +P A  G+TKF DL+ +EF  ++L          R  A   +      + +P   DWR+ GAVT VKDQGACGSCW+FSA     G
Sbjct:   38 FEEFKRTYGRAYETLAEEQQRLANFERNLELMREHQARNPHAQFGITKFFDLSEAEFAARYLNGAAYFAAAKRHAAQHYRKARADLSAVPDAVDWREKGAVTPVKDQGACGSCWAFSAVGNIEG 161

HSP 2 Score: 28.4906 bits (62), Expect = 8.137e-21
Identity = 15/37 (40.54%), Postives = 20/37 (54.05%), Query Frame = 2

Query:  614 SCDS---GCNGGVMDSAF*YLLQGGGGERKKGIPFPW 715
            SCD    GC+GG+M  AF +LLQ   G       +P+
Sbjct:  180 SCDDMNDGCDGGLMLQAFDWLLQNTNGHLHTEDSYPY 216

HSP 3 Score: 28.1054 bits (61), Expect = 8.137e-21
Identity = 14/23 (60.87%), Postives = 17/23 (73.91%), Query Frame = 1

Query:  505 GALEGAHFLSTGELVSLREQQLV 573
            G +EG  +L+  ELVSL EQQLV
Sbjct:  157 GNIEGQWYLAGHELVSLSEQQLV 179

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CATL_SCHMA (Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=1)

HSP 1 Score: 92.0485 bits (227), Expect = 1.186e-20
Identity = 50/124 (40.32%), Postives = 75/124 (60.48%), Query Frame = 3

Query:  144 NAEHHFSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQL-LDPTAVHGVTKFSDLTPSEFRRQFLGLNRRLRLPADAQKAPIL---PTNDLPTDFDWRDHGAVTGVKDQGACGSCWSFSAT 503
            N +  +  FK K+ K Y   E+ + RF +FK+N+ +A+  Q+ +  +A++GVT +SDLT  EF R  L  +    +P+     P       N++P +FDWR+ GAVT VK+QG CGSCW+FS T
Sbjct:   15 NVDEKYVQFKLKYRKQYHETED-EIRFNIFKSNILKAQLYQVFVRGSAIYGVTPYSDLTTDEFARTHLTAS--WVVPSSRSNTPTSLGKEVNNIPKNFDWREKGAVTEVKNQGMCGSCWAFSTT 135

HSP 2 Score: 29.261 bits (64), Expect = 1.186e-20
Identity = 14/24 (58.33%), Postives = 17/24 (70.83%), Query Frame = 1

Query:  505 GALEGAHFLSTGELVSLREQQLVD 576
            G +E   F  TG+L+SL EQQLVD
Sbjct:  136 GNVESQWFRKTGKLLSLSEQQLVD 159

[Close]

BLAST of EY665835 vs. ExPASy Swiss-Prot
Match: CATF_MOUSE (Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1)

HSP 1 Score: 100.523 bits (249), Expect = 1.289e-20
Identity = 57/129 (44.19%), Postives = 78/129 (60.47%), Query Frame = 3

Query:  159 FSLFKSKFSKTYATQEEHDYRFRVFKANLRRAKRRQLLDP-TAVHGVTKFSDLTPSEFRRQFLGLNRRLRLPADAQKAPILPTNDL-PTDFDWRDHGAVTGVKDQGACGSCWSFSATRRFGGSALLIDG 539
            F  F + +++TY ++EE  +R  VF  N+ RA++ Q LD  TA +G+TKFSDLT  EF   +L  N  L+  +  + +P    NDL P ++DWR  GAVT VK+QG CGSCW+FS T    G   L  G
Sbjct:  165 FKDFMTTYNRTYESREEAQWRLTVFARNMIRAQKIQALDRGTAQYGITKFSDLTEEEFHTIYL--NPLLQKESGRKMSPAKSINDLAPPEWDWRKKGAVTEVKNQGMCGSCWAFSVTGNVEGQWFLNRG 291

The following BLAST results are available for this feature:

BLAST of EY665835 vs. ExPASy Swiss-Prot

BLAST of EY665835 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 102


Match Name	E-value	Identity	Description
CYSP_PEA	3.139e-59	56.62	Cysteine proteinase 15A OS=Pisum sativum PE=2 SV=1	[more]
RD19A_ARATH	1.155e-53	49.60	Cysteine proteinase RD19a OS=Arabidopsis thaliana ...	[more]
A494_ARATH	2.663e-50	47.08	Probable cysteine proteinase A494 OS=Arabidopsis t...	[more]
CYSP1_MAIZE	7.252e-48	48.20	Cysteine proteinase 1 OS=Zea mays GN=CCP1 PE=2 SV=...	[more]
CYSP_TRYBB	1.988e-24	40.77	Cysteine proteinase OS=Trypanosoma brucei brucei P...	[more]
CPR1_DROME	1.637e-22	37.30	Putative cysteine proteinase CG12163 OS=Drosophila...	[more]
LMCPB_LEIME	8.137e-21	37.90	Cysteine proteinase B OS=Leishmania mexicana GN=LM...	[more]
CYSP2_LEIPI	8.137e-21	37.90	Cysteine proteinase 2 OS=Leishmania pifanoi GN=CYS...	[more]
CATL_SCHMA	1.186e-20	40.32	Cathepsin L OS=Schistosoma mansoni GN=CL1 PE=2 SV=...	[more]
CATF_MOUSE	1.289e-20	44.19	Cathepsin F OS=Mus musculus GN=Ctsf PE=2 SV=1	[more]

Pages

Properties


Property Name	Value
Genbank description	CS00-C1-102-006-B06-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>EY665835 ID=EY665835; Name=EY665835; organism=Citrus sinensis; type=EST; length=874bp
AAATGGAGCGCCTTATTCTCTCCTCTCTCCTCCTCCTCCTCCTATCTTCT
GTGCTTGCGTCCGCTGTAGCTGTCAACGACGACGATGCTATGATCAGACA
GGTCGTGCCGTCAGACGGCGAACAATCCGAAGATCATCTCCTGAACGCGG
AGCACCACTTCTCCCTCTTCAAGTCCAAATTCTCCAAGACTTACGCCACC
CAGGAGGAGCACGATTACCGATTCCGCGTGTTCAAGGCTAATCTGCGCCG
AGCAAAGCGACGCCAGCTCCTGGACCCCACTGCTGTCCACGGTGTCACCA
AGTTCTCCGACTTAACGCCGTCTGAATTCCGCCGTCAGTTCCTTGGCTTG
AATAGGCGGCTTCGGCTGCCAGCTGACGCTCAAAAGGCTCCTATTCTCCC
CACCAACGATCTTCCTACTGACTTTGACTGGCGTGATCACGGCGCCGTTA
CTGGCGTCAAAGACCAGGGCGCATGTGGATCGTGCTGGTCGTTTAGTGCA
ACCCGGCGCTTTGGAGGGAGCGCACTTCTTATCGACGGGCGAGCTTGTCA
GCCTCAGGGAGCAACAGCTTGTGGACTGGTGATCACGAGTGGTGATTCCA
AAGGAAATCTGGGTCATGTGACTCTGGGTGCAATGGTGGGGTTATGGACT
CTGCCTTTTGATACTTACTCCAGGGTGGGGGGGGTGAACGAAAAAAAGGG
ATACCCTTCCCCTGGGACCAAAGGGGGGTTCCTGGCAATTTTGACCAAAA
ACCAAAATTGGGTGGCACTTGGTTCTAATTTTCAGGGGGTTTTTTCCCCT
TGAGGAAAAGACAGAAGGGGGGGCCAATTTTGGGGGGAAAACTTGGCCCC
CCCCGGCGCGGGGGGGACACATCG