CK936630 | Citrus Genome Database

Overview


Name	CK936630
Unique Name	CK936630
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	771

Libraries


Library Name	Type
Developing fruit flavedo at 80 DAFB	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Alignments

Feature Name	Type	Location	Analysis
Csv1_Contig12923	contig	Csv1_Contig12923:1..759.	BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1

Homology

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHI2_BRANA (Endochitinase CH25 OS=Brassica napus PE=2 SV=1)

HSP 1 Score: 189.889 bits (481), Expect = 5.083e-69
Identity = 86/148 (58.11%), Postives = 105/148 (70.95%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K++   FF QT HETTGGW  AP G Y+WGYCF  E +P S+YC P+  +PC     YYGRGP+QLSWNYNY +C           NNPDL++ DPV++FK+AIWFWMT Q PKPSCH VI+ +W+PS  D  AGR+PGYG+ TNIIN
Sbjct:  123 KKEIAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPSSNYCSPSAEWPCASGKSYYGRGPMQLSWNYNYGQCGRAIGSDLL--NNPDLVSNDPVIAFKAAIWFWMTPQSPKPSCHAVIVGQWQPSDADRAAGRVPGYGVITNIIN 268

HSP 2 Score: 92.4337 bits (228), Expect = 5.083e-69
Identity = 41/61 (67.21%), Postives = 50/61 (81.97%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            +L  IISR  FDD+L++RND  CPARGFYTYDAFI AA++FPGFG +G+   RK+EIAAFF
Sbjct:   70 DLSGIISRSQFDDMLKHRNDNACPARGFYTYDAFINAAKSFPGFGTTGDTATRKKEIAAFF 130

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHIB_ARATH (Basic endochitinase B OS=Arabidopsis thaliana GN=CHI-B PE=1 SV=3)

HSP 1 Score: 194.897 bits (494), Expect = 6.627e-69
Identity = 90/148 (60.81%), Postives = 106/148 (71.62%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K++   FF QT HETTGGW  AP G Y+WGYCF  E +P SDYC+P+  +PC    +YYGRGP+QLSWNYNY  C           NNPDL+A D V++FK+AIWFWMTAQPPKPSCH VI  +W+PS  D  AGRLPGYG+ TNIIN
Sbjct:  138 KKEVAAFFGQTSHETTGGWATAPDGPYSWGYCFKQEQNPASDYCEPSATWPCASGKRYYGRGPMQLSWNYNYGLCGRAIGVDLL--NNPDLVANDAVIAFKAAIWFWMTAQPPKPSCHAVIAGQWQPSDADRAAGRLPGYGVITNIIN 283

HSP 2 Score: 87.0409 bits (214), Expect = 6.627e-69
Identity = 38/61 (62.30%), Postives = 49/61 (80.33%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            +L  IIS   FDD+L++RND  CPARGFYTY+AFI AA++FPGFG +G+   RK+E+AAFF
Sbjct:   85 DLSGIISSSQFDDMLKHRNDAACPARGFYTYNAFITAAKSFPGFGTTGDTATRKKEVAAFF 145

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHI1_TOBAC (Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1 SV=2)

HSP 1 Score: 191.045 bits (484), Expect = 5.543e-68
Identity = 90/148 (60.81%), Postives = 104/148 (70.27%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   FFAQT HETTGGW  AP G YAWGYC++ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PSA D  A RLPG+G+ TNIIN
Sbjct:  132 KREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSAGDRAANRLPGFGVITNIIN 277

HSP 2 Score: 87.8113 bits (216), Expect = 5.543e-68
Identity = 39/61 (63.93%), Postives = 49/61 (80.33%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            +LG IIS  MFD +L++RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFF
Sbjct:   79 DLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFF 139

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHI2_TOBAC (Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 2.091e-67
Identity = 89/148 (60.14%), Postives = 104/148 (70.27%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   FFAQT HETTGGW  AP G YAWGYC++ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFKSA+WFWMT Q PKPSCH+VII  W+PS+ D  A RLPG+G+ TNIIN
Sbjct:  127 KREIAAFFAQTSHETTGGWATAPDGPYAWGYCWLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDPVISFKSALWFWMTPQSPKPSCHDVIIGRWQPSSADRAANRLPGFGVITNIIN 272

HSP 2 Score: 87.8113 bits (216), Expect = 2.091e-67
Identity = 39/61 (63.93%), Postives = 49/61 (80.33%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            +LG IIS  MFD +L++RND  C  +GFY+Y+AFI AA++FPGFG SG+ T RKREIAAFF
Sbjct:   74 DLGSIISSSMFDQMLKHRNDNACQGKGFYSYNAFINAARSFPGFGTSGDTTARKREIAAFF 134

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHI4_SOLTU (Endochitinase 4 (Fragment) OS=Solanum tuberosum GN=CHTB4 PE=2 SV=1)

HSP 1 Score: 196.052 bits (497), Expect = 8.602e-66
Identity = 93/148 (62.84%), Postives = 105/148 (70.95%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   FFAQT HETTGGWP AP G YAWGYCF+ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFKSAIWFWMT Q PKPSCH+VII  W+PSA D  A RLPG+G+ TNIIN
Sbjct:  131 KREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSGQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDPVISFKSAIWFWMTPQSPKPSCHDVIIGRWQPSAADRAANRLPGFGVITNIIN 276

HSP 2 Score: 75.485 bits (184), Expect = 8.602e-66
Identity = 34/62 (54.84%), Postives = 47/62 (75.81%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARG-FYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            ++G +IS  MFD +L++RND  C  +  FY+Y+AFI AA++F GFG +G+ T RKREIAAFF
Sbjct:   77 DIGSVISNSMFDQMLKHRNDNACQGKNNFYSYNAFINAARSFGGFGTTGDTTARKREIAAFF 138

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHIB_VITVI (Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE=2 SV=1)

HSP 1 Score: 179.489 bits (454), Expect = 2.486e-65
Identity = 85/148 (57.43%), Postives = 99/148 (66.89%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   F AQT HETTGGW  AP G YAWGYC++ E   P  YC P+  +PC    KYYGRGPIQ+S+NYNY +            NNPDL+ATD V+SFK+A WFWMT Q PKPSCH VI   W PS  D +AGRLPG+G+ TNIIN
Sbjct:  117 KREIAAFLAQTSHETTGGWASAPDGPYAWGYCYLREQGSPGAYCVPSAQWPCAAGRKYYGRGPIQISYNYNYGQAGKAIGVDLV--NNPDLVATDAVISFKTAFWFWMTPQSPKPSCHNVITGGWTPSGADRSAGRLPGFGVITNIIN 262

HSP 2 Score: 90.5077 bits (223), Expect = 2.486e-65
Identity = 43/83 (51.81%), Postives = 58/83 (69.88%), Query Frame = 3

Query:  111 SAQNCGSGVVYXXXXXXXXXXXXELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 359
            +A  CGSG               ++G++I+R MF+D+L++RN+  CP +GFYTYDAFI AA+AFPGFG +G+ T RKREIAAF
Sbjct:   47 TADYCGSGC------QSQCSSTGDIGQLITRSMFNDMLKHRNEGSCPGKGFYTYDAFIAAAKAFPGFGTTGDTTTRKREIAAF 123

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHIT_SOLTU (Endochitinase OS=Solanum tuberosum PE=2 SV=1)

HSP 1 Score: 189.119 bits (479), Expect = 5.511e-65
Identity = 89/148 (60.14%), Postives = 103/148 (69.59%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   FFAQT HETTGGW  AP G YAWGYCF+ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATDPV+SFK+A+WFWMT Q PKPSCH+VII  W PS+ D  A RLPG+G+ TNIIN
Sbjct:  131 KREIAAFFAQTSHETTGGWASAPDGPYAWGYCFLRERGNPGDYCPPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDPVISFKTALWFWMTPQSPKPSCHDVIIGRWNPSSADRAANRLPGFGVITNIIN 276

HSP 2 Score: 79.7221 bits (195), Expect = 5.511e-65
Identity = 35/61 (57.38%), Postives = 46/61 (75.41%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            +LG  IS  MFD +L++RN+  C  + FY+Y+AFI AA++FPGFG SG+   RKREIAAFF
Sbjct:   78 DLGSAISNSMFDQMLKHRNENSCQGKNFYSYNAFINAARSFPGFGTSGDINARKREIAAFF 138

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHIT_PHAVU (Endochitinase OS=Phaseolus vulgaris PE=1 SV=1)

HSP 1 Score: 182.185 bits (461), Expect = 9.370e-65
Identity = 88/148 (59.46%), Postives = 100/148 (67.57%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCD--PNYPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   F  QT HETTGGW  AP G YAWGYCF+ E +P S YC   P +PC    +YYGRGPIQ+SWNYNY +C           N PDL+ATD V+SFKSA+WFWMTAQ PKPS H+VI   W PS+ DV A RLPGYG  TNIIN
Sbjct:  128 KREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERNP-STYCSATPQFPCAPGQQYYGRGPIQISWNYNYGQCGRAIGVDLL--NKPDLVATDSVISFKSALWFWMTAQSPKPSSHDVITSRWTPSSADVAARRLPGYGTVTNIIN 272

HSP 2 Score: 85.8853 bits (211), Expect = 9.370e-65
Identity = 38/60 (63.33%), Postives = 48/60 (80.00%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 359
            +L  +ISR  FD +L++RND  CPA+GFYTYDAFI AA+A+P FGN+G+   RKREIAAF
Sbjct:   75 DLSALISRSTFDQMLKHRNDGACPAKGFYTYDAFIAAAKAYPSFGNTGDTATRKREIAAF 134

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHI5_PHAVU (Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.593e-64
Identity = 88/148 (59.46%), Postives = 100/148 (67.57%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCD--PNYPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   F  QT HETTGGW  AP G YAWGYCF+ E +P S YC   P +PC    +YYGRGPIQ+SWNYNY +C           N PDL+ATD V+SFKSA+WFWMTAQ PKPS H+VI   W PS+ DV A RLPGYG  TNIIN
Sbjct:  127 KREIAAFLGQTSHETTGGWATAPDGPYAWGYCFVRERNP-SAYCSATPQFPCAPGQQYYGRGPIQISWNYNYGQCGRAIGVDLL--NKPDLVATDSVISFKSALWFWMTAQSPKPSSHDVITSRWTPSSADVAARRLPGYGTVTNIIN 271

HSP 2 Score: 85.5001 bits (210), Expect = 1.593e-64
Identity = 38/60 (63.33%), Postives = 48/60 (80.00%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARGFYTYDAFIEAAQAFPGFGNSGNETMRKREIAAF 359
            +L  +ISR  FD +L++RND  CPA+GFYTYDAFI AA+A+P FGN+G+   RKREIAAF
Sbjct:   74 DLSALISRSTFDQVLKHRNDGACPAKGFYTYDAFIAAAKAYPSFGNTGDTATRKREIAAF 133

[Close]

BLAST of CK936630 vs. ExPASy Swiss-Prot
Match: CHI1_SOLTU (Endochitinase 1 (Fragment) OS=Solanum tuberosum GN=CHTB1 PE=2 SV=1)

HSP 1 Score: 188.348 bits (477), Expect = 1.594e-64
Identity = 89/148 (60.14%), Postives = 102/148 (68.92%), Query Frame = 1

Query:  340 KEKSLPFFAQTGHETTGGWPDAPGGEYAWGYCFISEVSPPSDYCDPN--YPCRG--KYYGRGPIQLSWNYNYLRCXXXXXXXXXXXNNPDLLATDPVLSFKSAIWFWMTAQPPKPSCHEVIIDEWKPSANDVNAGRLPGYGLTTNIIN 771
            K +   FFAQT HETTGGWP AP G YAWGYCF+ E   P DYC P+  +PC    KY+GRGPIQ+S NYNY  C           NNPDL+ATD V+SFKSAIWFWMT Q PKPSCH+VI   W+PS  D  A R+PG+G+ TNIIN
Sbjct:  121 KREIAAFFAQTSHETTGGWPTAPDGPYAWGYCFLREQGSPGDYCTPSSQWPCAPGRKYFGRGPIQISHNYNYGPCGRAIGVDLL--NNPDLVATDSVISFKSAIWFWMTPQSPKPSCHDVITGRWQPSGVDQAANRVPGFGVITNIIN 266

HSP 2 Score: 78.9518 bits (193), Expect = 1.594e-64
Identity = 37/62 (59.68%), Postives = 47/62 (75.81%), Query Frame = 3

Query:  180 ELGKIISREMFDDLLEYRNDERCPARG-FYTYDAFIEAAQAFPGFGNSGNETMRKREIAAFF 362
            +LG +IS  MFD +L +RND  C  +G FY+Y+AFI AA +FPGFG +G+ T RKREIAAFF
Sbjct:   67 DLGGVISNSMFDQMLNHRNDNACQGKGNFYSYNAFISAAGSFPGFGTTGDITARKREIAAFF 128

The following BLAST results are available for this feature:

BLAST of CK936630 vs. ExPASy Swiss-Prot

BLAST of CK936630 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 55


Match Name	E-value	Identity	Description
CHI2_BRANA	5.083e-69	58.11	Endochitinase CH25 OS=Brassica napus PE=2 SV=1	[more]
CHIB_ARATH	6.627e-69	60.81	Basic endochitinase B OS=Arabidopsis thaliana GN=C...	[more]
CHI1_TOBAC	5.543e-68	60.81	Endochitinase A OS=Nicotiana tabacum GN=CHN48 PE=1...	[more]
CHI2_TOBAC	2.091e-67	60.14	Endochitinase B OS=Nicotiana tabacum GN=CHN50 PE=1...	[more]
CHI4_SOLTU	8.602e-66	62.84	Endochitinase 4 (Fragment) OS=Solanum tuberosum GN...	[more]
CHIB_VITVI	2.486e-65	57.43	Basic endochitinase OS=Vitis vinifera GN=CHIT1B PE...	[more]
CHIT_SOLTU	5.511e-65	60.14	Endochitinase OS=Solanum tuberosum PE=2 SV=1	[more]
CHIT_PHAVU	9.370e-65	59.46	Endochitinase OS=Phaseolus vulgaris PE=1 SV=1	[more]
CHI5_PHAVU	1.593e-64	59.46	Endochitinase CH5B OS=Phaseolus vulgaris PE=3 SV=1	[more]
CHI1_SOLTU	1.594e-64	60.14	Endochitinase 1 (Fragment) OS=Solanum tuberosum GN...	[more]

Pages

Properties


Property Name	Value
Genbank description	CGF1004520_A04 Developing fruit flavedo at 80 DAFB Citrus sinensis cDNA clone F80DAB0001_IIIF_A04 5, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CK936630 ID=CK936630; Name=CK936630; organism=Citrus sinensis; type=EST; length=771bp
GGGGGAACACCAAGCAGAGGATAATTGATATCATAACTGNNNNTTTCAAA
ATGAGGCTTATTGGGTCCTTACTAATTTTCTCTCTAGTTTTATCTTTTGT
ACTAGGAGGCTCAGCACAGAACTGCGGAAGCGGCGTTGTGTACGGTGGAC
GTGATACCGGCCATGGGACGCATGGCGGTGAACTGGGCAAGATCATCTCA
AGGGAGATGTTTGATGACTTGCTTGAGTATAGAAATGATGAACGATGCCC
TGCCAGAGGCTTCTACACTTATGATGCTTTTATAGAGGCAGCCCAAGCTT
TTCCAGGCTTTGGTAACTCCGGAAATGAAACCATGCGTAAAAGAGAAATC
GCTGCCTTTTTTTGCCCAAACTGGCCATGAAACTACTGGGGGATGGCCCG
ATGCACCTGGTGGCGAATATGCTTGGGGATATTGCTTTATAAGCGAAGTT
AGCCCTCCGTCCGACTACTGTGATCCTAACTATCCATGTCGAGGGAAGTA
CTATGGCCGAGGTCCAATTCAACTCTCTTGGAATTACAATTACTTACGGT
GCGGAGAAGGCCTAGGGCTAGGAGAGGAGTTGTTGAACAATCCAGACCTT
CTTGCTACTGATCCAGTACTATCATTCAAGTCAGCAATCTGGTTCTGGAT
GACTGCACAGCCACCAAAGCCATCGTGCCACGAAGTCATTATCGACGAAT
GGAAACCATCAGCAAATGACGTAAACGCCGGCCGGCTTCCGGGATACGGT
CTAACCACAAATATAATCAAC