EY665609

Overview
NameEY665609
Unique NameEY665609
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length849
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY665609_ssr93 microsatellite EY665609_ssr93:93..106. BLAST: Citrus ESTs to Prunus persica proteins V1
Csv1_Contig1286 contig Csv1_Contig1286:19..869. BLAST: Citrus Unigene V1 Contigs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1_TOBAC (Glutamate--cysteine ligase, chloroplastic OS=Nicotiana tabacum GN=GSH1 PE=2 SV=2)

HSP 1 Score: 200.29 bits (508), Expect = 3.052e-61
Identity = 108/178 (60.67%), Postives = 122/178 (68.54%), Query Frame = 2 
Query:  203 MTLISRASPSCRVLTDVTRCTIRRSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            M L+S+A  S  + ++  +C    SG   +  N E  +MK+ C   + SSR S+K  Q ++LD             VAASPPTEDAVV  EPLTKEDLV YLASGCK KEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLN IAERFDWEKVMEGD IIGLKQGKQ+  L   G   L
Sbjct:    1 MALMSQAGSSHCIYSEKMKCI---SGHSSITSNMEMLKMKDICFG-NISSRNSSKPMQGIYLDRVGVERRRGRLAIVAASPPTEDAVVAAEPLTKEDLVAYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNGIAERFDWEKVMEGDNIIGLKQGKQSISLEPGGQFEL 174

HSP 2 Score: 56.9954 bits (136), Expect = 3.052e-61
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  176 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIG 206
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1_SOLLC (Glutamate--cysteine ligase, chloroplastic OS=Solanum lycopersicum GN=GSH1 PE=2 SV=1)

HSP 1 Score: 197.978 bits (502), Expect = 1.499e-60
Identity = 105/178 (58.99%), Postives = 122/178 (68.54%), Query Frame = 2 
Query:  203 MTLISRASPSCRVLTDVTRCTIRRSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            M L+S+A  S  + ++  RC    SG   +  N +  RM+E C     SSR +++  Q  +L++            VAASPPTEDAVV  EPLTKEDLVGYLASGCK KEKWRIGTEHEKFGFEFGTLRPMKY+QIA+LLN IAERFDWEKVMEGD IIGLKQGKQ+  L   G   L
Sbjct:    1 MALMSQAGSSHCIYSEKVRCI---SGHRSIINNMDMFRMREICFGVDISSRNASRRVQGNYLNHIGVGSRRGDLTIVAASPPTEDAVVAAEPLTKEDLVGYLASGCKSKEKWRIGTEHEKFGFEFGTLRPMKYDQIADLLNGIAERFDWEKVMEGDKIIGLKQGKQSISLEPGGQFEL 175

HSP 2 Score: 56.9954 bits (136), Expect = 1.499e-60
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  177 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIG 207
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1_ARATH (Glutamate--cysteine ligase, chloroplastic OS=Arabidopsis thaliana GN=GSH1 PE=1 SV=2)

HSP 1 Score: 174.096 bits (440), Expect = 2.076e-53
Identity = 100/179 (55.87%), Postives = 120/179 (67.04%), Query Frame = 2 
Query:  203 MTLISRASPSCRVL-TDVTRCTIRRSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            M L+S+A  S  V+ + V      ++ V G  +N +  RMKE   +F SS  YS  +S    L ++           VAASPPTE+AVV TEPLT+EDL+ YLASGCK K+K+RIGTEHEKFGFE  TLRPMKY+QIAELLN IAERF+WEKVMEGD IIGLKQGKQ+  L   G   L
Sbjct:    1 MALLSQAGGSYTVVPSGVCSKAGTKAVVSGGVRNLDVLRMKE---AFGSS--YSRSLSTKSMLLHSVKRSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKTKDKYRIGTEHEKFGFEVNTLRPMKYDQIAELLNGIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFEL 174

HSP 2 Score: 56.9954 bits (136), Expect = 2.076e-53
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  176 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIG 206
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1_BRAJU (Glutamate--cysteine ligase, chloroplastic OS=Brassica juncea GN=GSH1 PE=1 SV=1)

HSP 1 Score: 172.94 bits (437), Expect = 4.599e-53
Identity = 93/155 (60.00%), Postives = 104/155 (67.10%), Query Frame = 2 
Query:  272 RSGVCGLAKNAEASRMKETCVSFSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            R+G   ++      RMKET VS           S+ L   +            VAASPPTE+AVV TEPLT+EDL+ YLASGCK KEKWRIGTEHEKFGFE  TLRPMKY+QIAELLNSIAERF+WEKVMEGD IIGLKQGKQ+  L   G   L
Sbjct:   21 RTGTKTVSGCVNVLRMKETYVS---------SYSRTLSTKSMLKRSKRGHQLIVAASPPTEEAVVATEPLTREDLIAYLASGCKSKEKWRIGTEHEKFGFEVNTLRPMKYDQIAELLNSIAERFEWEKVMEGDKIIGLKQGKQSISLEPGGQFEL 166

HSP 2 Score: 56.9954 bits (136), Expect = 4.599e-53
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  168 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIG 198
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1_MEDTR (Glutamate--cysteine ligase, chloroplastic OS=Medicago truncatula GN=GSH1 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.328e-52
Identity = 89/142 (62.68%), Postives = 104/142 (73.24%), Query Frame = 2 
Query:  317 MKETCVS--FSSSSRYSAKISQVLHLDNAXXXXXXXXXXXVAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            +++T +S  FS SS+ S    ++L    +           VAASPPTEDAVV TEPLTK+DL+ YLASGCK K+KWRIGTEHEKFGFE G+LRPMKYEQI+ELLN IAERFDW+KVMEGD IIGLKQGKQ+  L   G   L
Sbjct:   26 IRKTSISNTFSFSSKNSLSFKRILTSGGSRRFI-------VAASPPTEDAVVATEPLTKQDLIDYLASGCKTKDKWRIGTEHEKFGFELGSLRPMKYEQISELLNGIAERFDWDKVMEGDNIIGLKQGKQSISLEPGGQFEL 160

HSP 2 Score: 56.9954 bits (136), Expect = 1.328e-52
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  162 GAPLETLHQTCAEVNSHLYQVKAVAEEMGIG 192
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSJ (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-1 PE=2 SV=1)

HSP 1 Score: 154.451 bits (389), Expect = 3.408e-47
Identity = 71/102 (69.61%), Postives = 84/102 (82.35%), Query Frame = 2 
Query:  431 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            VAASPPTE+AV +TEPLTKEDLV YL SGCKPKE WRIGTEHEKFGFE  TLRP+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQ+  L   G   L
Sbjct:   43 VAASPPTEEAVQMTEPLTKEDLVAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFEL 144

HSP 2 Score: 55.8398 bits (133), Expect = 3.408e-47
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  146 GAPLETLHQTCAEVNSHLYQVKAVGEEMGIG 176
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1A_ORYSI (Glutamate--cysteine ligase A, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-1 PE=2 SV=2)

HSP 1 Score: 154.451 bits (389), Expect = 3.408e-47
Identity = 71/102 (69.61%), Postives = 84/102 (82.35%), Query Frame = 2 
Query:  431 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            VAASPPTE+AV +TEPLTKEDLV YL SGCKPKE WRIGTEHEKFGFE  TLRP+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQ+  L   G   L
Sbjct:   43 VAASPPTEEAVQMTEPLTKEDLVAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFEL 144

HSP 2 Score: 55.8398 bits (133), Expect = 3.408e-47
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  146 GAPLETLHQTCAEVNSHLYQVKAVGEEMGIG 176
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSJ (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. japonica GN=GSH1-2 PE=3 SV=1)

HSP 1 Score: 153.295 bits (386), Expect = 7.544e-47
Identity = 70/102 (68.63%), Postives = 84/102 (82.35%), Query Frame = 2 
Query:  431 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            VAASPPTE+AV +TEPLTKEDL+ YL SGCKPKE WRIGTEHEKFGFE  TLRP+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQ+  L   G   L
Sbjct:   47 VAASPPTEEAVQMTEPLTKEDLMAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFEL 148

HSP 2 Score: 55.8398 bits (133), Expect = 7.544e-47
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  150 GAPLETLHQTCAEVNSHLYQVKAVGEEMGIG 180
[Close]
BLAST of EY665609 vs. ExPASy Swiss-Prot
Match: GSH1B_ORYSI (Glutamate--cysteine ligase B, chloroplastic OS=Oryza sativa subsp. indica GN=GSH1-2 PE=3 SV=2)

HSP 1 Score: 153.295 bits (386), Expect = 7.544e-47
Identity = 70/102 (68.63%), Postives = 84/102 (82.35%), Query Frame = 2 
Query:  431 VAASPPTEDAVVVTEPLTKEDLVGYLASGCKPKEKWRIGTEHEKFGFEFGTLRPMKYEQIAELLNSIAERFDWEKVMEGDYIIGLKQGKQAYHLNLAGSLSL 736
            VAASPPTE+AV +TEPLTKEDL+ YL SGCKPKE WRIGTEHEKFGFE  TLRP+KY+QI ++LN +AERFDW+K++E + +IGLKQGKQ+  L   G   L
Sbjct:   47 VAASPPTEEAVQMTEPLTKEDLMAYLVSGCKPKENWRIGTEHEKFGFEVDTLRPIKYDQIRDILNGLAERFDWDKIVEENNVIGLKQGKQSISLEPGGQFEL 148

HSP 2 Score: 55.8398 bits (133), Expect = 7.544e-47
Identity = 27/31 (87.10%), Postives = 27/31 (87.10%), Query Frame = 3 
Query:  738 GAPLETLHQTCAEVISHLYQGKACCEEMGIG 830
            GAPLETLHQTCAEV SHLYQ KA  EEMGIG
Sbjct:  150 GAPLETLHQTCAEVNSHLYQVKAVGEEMGIG 180
The following BLAST results are available for this feature:
BLAST of EY665609 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 9
Match NameE-valueIdentityDescription
GSH1_TOBAC3.052e-6160.67Glutamate--cysteine ligase, chloroplastic OS=Nicot... [more]
GSH1_SOLLC1.499e-6058.99Glutamate--cysteine ligase, chloroplastic OS=Solan... [more]
GSH1_ARATH2.076e-5355.87Glutamate--cysteine ligase, chloroplastic OS=Arabi... [more]
GSH1_BRAJU4.599e-5360.00Glutamate--cysteine ligase, chloroplastic OS=Brass... [more]
GSH1_MEDTR1.328e-5262.68Glutamate--cysteine ligase, chloroplastic OS=Medic... [more]
GSH1A_ORYSJ3.408e-4769.61Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
GSH1A_ORYSI3.408e-4769.61Glutamate--cysteine ligase A, chloroplastic OS=Ory... [more]
GSH1B_ORYSJ7.544e-4768.63Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
GSH1B_ORYSI7.544e-4768.63Glutamate--cysteine ligase B, chloroplastic OS=Ory... [more]
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Properties
Property NameValue
Genbank descriptionCS00-C1-102-070-E09-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665609 ID=EY665609; Name=EY665609; organism=Citrus sinensis; type=EST; length=849bp
GTTTTTCGGGGGGGCGAAGCGCAGTACGGTCGGGATTCCGGGTCGACCCC
GCGTCCGTCTCTCTGCATTCTGCAAGTGAGTCTTCGCTGTCCTATATATA
TATATAACTCAACGAGTGTTTTATCTTCTACGAAATTCGAAACCCTAGTT
TTTAAGATTCCAATTGTGGTAATTCGTTCAGCGTTAACCAAGCGGGAGCA
GTATGACTCTCATTTCGCGGGCCAGTCCATCATGTCGTGTATTGACTGAT
GTAACACGATGTACAATCAGACGAAGTGGGGTTTGTGGCTTGGCAAAAAA
TGCTGAGGCATCTAGGATGAAGGAAACTTGTGTTAGTTTCTCTTCCTCAT
CACGCTATTCTGCCAAGATATCACAAGTTTTACATCTAGACAATGCAGGG
ATAGGAATTCGAAGAGGACGTCGATCTATTGTTGCAGCTAGTCCTCCCAC
GGAGGATGCTGTTGTTGTAACGGAGCCATTGACCAAAGAGGATCTAGTTG
GATACCTTGCCTCTGGGTGCAAGCCTAAGGAAAAGTGGAGAATTGGTACA
GAACATGAGAAGTTTGGTTTTGAGTTTGGAACTTTACGTCCTATGAAATA
CGAACAAATAGCCGAGTTGCTTAACAGCATTGCTGAGAGGTTCGATTGGG
AGAAAGTAATGGAAGGAGATTACATCATAGGACTGAAACAGGGGAAACAA
GCATATCACTTGAACCTGGCGGGCAGTTTGAGCTTGTGGTGCACCTCTTG
AAACTCTGCATCAAACTTGTGCTGAAGTTATTTCACACCTATATCAGGGG
AAAGCTTGTTGCGAGGAAATGGGAATTGGGATTTTTAAGAATTGGGCTC
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