EY665532

Overview
NameEY665532
Unique NameEY665532
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length910
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Homology
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: YI01_SCHPO (Putative amidase C869.01 OS=Schizosaccharomyces pombe GN=SPAC869.01 PE=2 SV=1)

HSP 1 Score: 182.956 bits (463), Expect = 1.420e-50
Identity = 96/189 (50.79%), Postives = 130/189 (68.78%), Query Frame = 3 
Query:  102 IREATINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPDALSQADEADCERRVKAPGSLPG-LHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPS 665
            + +ATI+ LQ   +   LTS  +V  YL    ++NP +NG++++NPD L+ A E D ER   A G + G LHGIP ++KDN ATKDKM+TTAGSYALLGS+VPRDA VV +LREAGA++ G A+LSEWA+ RS++   G+ ARGGQ + P+ L+ +P             N++A ALGTETDGSI+ P+
Sbjct:   66 LEDATIDQLQNYMENGILTSTDIVHCYLDRYLQVNPYVNGILQLNPDVLTIASELDDER---ANGIIRGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTETDGSIIDPA 251

HSP 2 Score: 38.891 bits (89), Expect = 1.420e-50
Identity = 18/29 (62.07%), Postives = 21/29 (72.41%), Query Frame = 2 
Query:  671 NSVVGIKPTVGLTRRAGGIPLTPRQDSVG 757
            N VVG+KPTVGLT R G IP +  QD+ G
Sbjct:  254 NGVVGLKPTVGLTSRYGVIPESEHQDTTG 282
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_THEPX (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) GN=gatA PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.531e-19
Identity = 65/186 (34.95%), Postives = 102/186 (54.84%), Query Frame = 3 
Query:  114 TINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            TI++L+   K+ ++++ ++ + YL  I  + P ++ ++ +  D AL +A EAD   ++K  G    L GIP+++KDNI+T+  + TT  S  L   + P +A VV KL E G IILGK++L E+A   SS   + F       KNP+ LS  P             +  A ALG++T GSI  P+S
Sbjct:    7 TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEAD--EKIKK-GEDTALTGIPVIIKDNISTEG-IKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNLDEFA-MGSSTENSAFKTT----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQPAS 183

HSP 2 Score: 26.9498 bits (58), Expect = 6.531e-19
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  677 VVGIKPTVGLTRRAGGIPLTPRQDSVG 757
            VVG+KPT GL  R G +      D +G
Sbjct:  187 VVGMKPTYGLVSRYGLVAFASSLDQIG 213
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_THEP3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) GN=gatA PE=3 SV=1)

HSP 1 Score: 88.5817 bits (218), Expect = 6.531e-19
Identity = 66/186 (35.48%), Postives = 102/186 (54.84%), Query Frame = 3 
Query:  114 TINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            TI++L+   K+ ++++ ++ + YL  I  + P ++ +I +  D AL +A EAD   ++K  G    L GIP+++KDNI+T+  + TT  S  L   + P +A VV KL E G IILGK++L E+A   SS   + F       KNP+ LS  P             +  A ALG++T GSI  P+S
Sbjct:    7 TIHELRELLKKREVSALEVTKSYLERIKEVEPKIDALITITEDFALQRAKEAD--EKIKN-GEDTALTGIPVIIKDNISTEG-IKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNLDEFA-MGSSTENSAFKTT----KNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQPAS 183

HSP 2 Score: 26.9498 bits (58), Expect = 6.531e-19
Identity = 12/27 (44.44%), Postives = 15/27 (55.56%), Query Frame = 2 
Query:  677 VVGIKPTVGLTRRAGGIPLTPRQDSVG 757
            VVG+KPT GL  R G +      D +G
Sbjct:  187 VVGMKPTYGLVSRYGLVAFASSLDQIG 213
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_CHRSD (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Chromohalobacter salexigens (strain DSM 3043 / ATCC BAA-138 / NCIMB 13768) GN=gatA PE=3 SV=1)

HSP 1 Score: 93.9745 bits (232), Expect = 1.290e-18
Identity = 68/190 (35.79%), Postives = 98/190 (51.58%), Query Frame = 3 
Query:  102 IREATINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            + + T+ +L    +  + +S +L +  L  I RL+  LN  I V  + AL+ AD AD   + +A G    L G+P+ LKD   T+  + T+ GS  L   V P DA VV K+R+AG + LGK ++ E+A    S+  N F    G  KNP+ LSA P              LV  ALGT+T GSI  P++
Sbjct:    1 MHDKTLTELAAGLRAGEFSSRELCQSALDRIQRLDGDLNSFISVTAEQALADADAAD---QARASGDAAALAGLPLALKDIFCTQG-VRTSCGSKMLDSFVAPYDATVVEKIRQAGGVSLGKTNMDEFA--MGSSNENSFY---GPVKNPWDLSAVPGGSSGGSAAAVAAGLVPAALGTDTGGSIRQPAA 181
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_PROMT (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Prochlorococcus marinus (strain NATL2A) GN=gatA PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.436e-18
Identity = 64/186 (34.41%), Postives = 99/186 (53.23%), Query Frame = 3 
Query:  114 TINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            T  DL+   K  +++S +LV+  +  I+ L+P LN  + VN + ALS+A+  D  +++ +   LP L GIPI +KDN+ TK  + TT  S  L   V P ++ V  KL  AGAI++GK ++ E+A   SS   + F    G   NP+ ++  P              L   +LG++T GSI  P+S
Sbjct:    2 TFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHID--KQIASGDHLPPLSGIPIAIKDNLCTKG-IKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMDEFA-MGSSTETSAF----GPTLNPWNITKVPGGSSGGSAASVAAGLCYGSLGSDTGGSIRQPAS 179

HSP 2 Score: 25.0238 bits (53), Expect = 1.436e-18
Identity = 13/27 (48.15%), Postives = 14/27 (51.85%), Query Frame = 2 
Query:  677 VVGIKPTVGLTRRAGGIPLTPRQDSVG 757
            VVG+KPT G   R G I      D VG
Sbjct:  183 VVGMKPTYGRVSRWGLIAFASSLDQVG 209
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_PROM1 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Prochlorococcus marinus (strain NATL1A) GN=gatA PE=3 SV=1)

HSP 1 Score: 89.3521 bits (220), Expect = 1.436e-18
Identity = 64/186 (34.41%), Postives = 99/186 (53.23%), Query Frame = 3 
Query:  114 TINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            T  DL+   K  +++S +LV+  +  I+ L+P LN  + VN + ALS+A+  D  +++ +   LP L GIPI +KDN+ TK  + TT  S  L   V P ++ V  KL  AGAI++GK ++ E+A   SS   + F    G   NP+ ++  P              L   +LG++T GSI  P+S
Sbjct:    2 TFEDLRQKLKSGEVSSKELVQEKINRINELDPTLNSFLIVNTELALSKAEHID--KQIASGDHLPPLSGIPIAIKDNLCTKG-IKTTCASKILDNFVPPYESTVTKKLLNAGAIMIGKTNMDEFA-MGSSTETSAF----GPTLNPWNITKVPGGSSGGSAASVAAGLCYGSLGSDTGGSIRQPAS 179

HSP 2 Score: 25.0238 bits (53), Expect = 1.436e-18
Identity = 13/27 (48.15%), Postives = 14/27 (51.85%), Query Frame = 2 
Query:  677 VVGIKPTVGLTRRAGGIPLTPRQDSVG 757
            VVG+KPT G   R G I      D VG
Sbjct:  183 VVGMKPTYGRVSRWGLIAFASSLDQVG 209
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_COXBU (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii GN=gatA PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.684e-18
Identity = 68/190 (35.79%), Postives = 97/190 (51.05%), Query Frame = 3 
Query:  102 IREATINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            + + TI +L+   +   ++S +L + +L  I  +NP LN  I +  + AL+QA  AD      A G    L GIPI  KD   TKD + T+ GS  L   + P DA VV +L +AGAI++GK ++ E+A   SSN  + F    G  KNP+ L   P              LV  A GT+T GSI  P++
Sbjct:    1 MHQKTIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARL---AKGEATSLTGIPIAQKDIFCTKD-IKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDEFA-MGSSNENSYF----GAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAA 181
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_COXBR (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain RSA 331 / Henzerling II) GN=gatA PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.684e-18
Identity = 68/190 (35.79%), Postives = 97/190 (51.05%), Query Frame = 3 
Query:  102 IREATINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            + + TI +L+   +   ++S +L + +L  I  +NP LN  I +  + AL+QA  AD      A G    L GIPI  KD   TKD + T+ GS  L   + P DA VV +L +AGAI++GK ++ E+A   SSN  + F    G  KNP+ L   P              LV  A GT+T GSI  P++
Sbjct:    1 MHQKTIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARL---AKGEATSLTGIPIAQKDIFCTKD-IKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDEFA-MGSSNENSYF----GAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAA 181
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_COXBN (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain Dugway 5J108-111) GN=gatA PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.684e-18
Identity = 68/190 (35.79%), Postives = 97/190 (51.05%), Query Frame = 3 
Query:  102 IREATINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            + + TI +L+   +   ++S +L + +L  I  +NP LN  I +  + AL+QA  AD      A G    L GIPI  KD   TKD + T+ GS  L   + P DA VV +L +AGAI++GK ++ E+A   SSN  + F    G  KNP+ L   P              LV  A GT+T GSI  P++
Sbjct:    1 MHQKTIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARL---AKGEATSLTGIPIAQKDIFCTKD-IKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDEFA-MGSSNENSYF----GAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAA 181
[Close]
BLAST of EY665532 vs. ExPASy Swiss-Prot
Match: GATA_COXB2 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Coxiella burnetii (strain CbuG_Q212) GN=gatA PE=3 SV=1)

HSP 1 Score: 93.5893 bits (231), Expect = 1.684e-18
Identity = 68/190 (35.79%), Postives = 97/190 (51.05%), Query Frame = 3 
Query:  102 IREATINDLQLAFKQNKLTSWQLVEFYLGEIHRLNPVLNGVIEVNPD-ALSQADEADCERRVKAPGSLPGLHGIPILLKDNIATKDKMNTTAGSYALLGSVVPRDAGVVTKLREAGAIILGKASLSEWANFRSSNAPNGFCARGGQGKNPYVLSADPWXXXXXXXXXXXXNLVAVALGTETDGSILSPSS 668
            + + TI +L+   +   ++S +L + +L  I  +NP LN  I +  + AL+QA  AD      A G    L GIPI  KD   TKD + T+ GS  L   + P DA VV +L +AGAI++GK ++ E+A   SSN  + F    G  KNP+ L   P              LV  A GT+T GSI  P++
Sbjct:    1 MHQKTIAELKQDLRDKTISSVELTQHFLDRIKTINPTLNSFISITEEYALTQAKAADARL---AKGEATSLTGIPIAQKDIFCTKD-IKTSCGSKMLDNFIAPYDATVVEQLNKAGAILIGKTNMDEFA-MGSSNENSYF----GAVKNPWDLERVPGGSSGGSAAAVAARLVPGATGTDTGGSIRQPAA 181
The following BLAST results are available for this feature:
BLAST of EY665532 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 246
Match NameE-valueIdentityDescription
YI01_SCHPO1.420e-5050.79Putative amidase C869.01 OS=Schizosaccharomyces po... [more]
GATA_THEPX6.531e-1934.95Glutamyl-tRNA(Gln) amidotransferase subunit A OS=T... [more]
GATA_THEP36.531e-1935.48Glutamyl-tRNA(Gln) amidotransferase subunit A OS=T... [more]
GATA_CHRSD1.290e-1835.79Glutamyl-tRNA(Gln) amidotransferase subunit A OS=C... [more]
GATA_PROMT1.436e-1834.41Glutamyl-tRNA(Gln) amidotransferase subunit A OS=P... [more]
GATA_PROM11.436e-1834.41Glutamyl-tRNA(Gln) amidotransferase subunit A OS=P... [more]
GATA_COXBU1.684e-1835.79Glutamyl-tRNA(Gln) amidotransferase subunit A OS=C... [more]
GATA_COXBR1.684e-1835.79Glutamyl-tRNA(Gln) amidotransferase subunit A OS=C... [more]
GATA_COXBN1.684e-1835.79Glutamyl-tRNA(Gln) amidotransferase subunit A OS=C... [more]
GATA_COXB21.684e-1835.79Glutamyl-tRNA(Gln) amidotransferase subunit A OS=C... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-102-001-D08-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 2 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY665532 ID=EY665532; Name=EY665532; organism=Citrus sinensis; type=EST; length=910bp
CCACGCGTCCGAATAATGTGTCATTGACCTTTCCTTACTTTTCACATCTA
CTCCTGATTCTTCTAGCCATGTCAACCGATCGATCCGACGATGAATTCCC
AATAAGAGAGGCAACTATCAACGATCTCCAGCTCGCTTTCAAGCAAAACA
AGCTTACGTCCTGGCAGCTGGTTGAATTTTACCTCGGAGAAATCCACAGG
CTCAACCCGGTGCTCAACGGAGTCATAGAGGTGAACCCTGATGCACTATC
CCAAGCTGATGAAGCTGACTGCGAGCGCAGGGTTAAGGCACCAGGTTCAC
TGCCCGGTTTACATGGGATTCCTATTCTTCTCAAGGATAACATTGCAACG
AAGGACAAGATGAACACCACTGCTGGCTCGTATGCTCTGCTCGGCTCAGT
CGTGCCCCGGGATGCTGGTGTGGTGACCAAATTGAGGGAAGCTGGTGCCA
TTATTCTGGGAAAGGCTAGCTTGAGTGAGTGGGCTAATTTTAGATCCTCA
AATGCACCCAATGGTTTCTGTGCTAGGGGTGGCCAGGGAAAGAATCCATA
TGTTCTATCAGCAGATCCCTGGGGGTCGAGCAGTGGCTCAGCAATATCGG
TAGCAGGCAACTTGGTTGCAGTGGCACTGGGAACTGAAACTGATGGGTCA
ATTCTCTCTCCTTCCAGTCCAACTCAGTAGTGGGAATCAAACCAACGGTT
GGGCTCACCCGCAGAGCCGGCGGCATCCCTTTAACTCCTAGGCAGGACTC
CGTTGGGGAATGGGAACCTTTGCCATATCATTTTGATGGCCGTTACCCTT
TAATTGACAAGGGACTATCTGCCCCGGGAGGCCTTATTTGCAAACTGGGA
GCGGAGGCTGCTTTATGGTGCTGGACGCCACTGGCGGGGATTGGTTCCCG
AGGAGCCCGG
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