CX673309 | Citrus Genome Database

Overview


Name	CX673309
Unique Name	CX673309
Type	EST
Organism	Citrus sinensis (Sweet orange)
Sequence length	899

Libraries


Library Name	Type
Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10	cdna_library

Analyses

This EST is derived from or has results from the following analyses


Analysis Name	Date Performed
BLAST: Citrus ESTs to Prunus persica proteins V1	2010-05-10
BLAST: Citrus ESTs to Populus V2 proteins	2010-05-10
BLAST: Citrus ESTs to TAIR9	2010-05-10
BLAST: Citrus ESTs to SwissProt	2010-05-10

Homology

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_SCHPO (Probable T-complex protein 1 subunit beta OS=Schizosaccharomyces pombe GN=cct2 PE=2 SV=1)

HSP 1 Score: 152.525 bits (384), Expect = 3.006e-36
Identity = 80/117 (68.38%), Postives = 93/117 (79.49%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            +ISKVQDDEVGDGTTSV V A ELLR+AE +V AKIHP  II G+R+A + A DAL    +DN  +  KF+SDL  IA TTLSSKILSQ+K HF QLAVDAV+RLKGSTNL++IQII
Sbjct:   82 NISKVQDDEVGDGTTSVCVFAAELLRQAEIMVNAKIHPQVIIDGYRIATKTAIDALRASSIDNSSDPAKFRSDLENIARTTLSSKILSQNKNHFAQLAVDAVLRLKGSTNLDNIQII 198

HSP 2 Score: 103.605 bits (257), Expect = 1.602e-21
Identity = 52/83 (62.65%), Postives = 66/83 (79.52%), Query Frame = 2

Query:   62 SMGVERIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLVGI 310
            S+   +IF +   +E+GE AR++SFVGA+A+ DLVK+TLGPKGMDKILQS   G ++ VTNDGATILKS+ +DN AAKVLV I
Sbjct:    2 SLNPHQIFNESGIQERGENARLSSFVGAIAVGDLVKSTLGPKGMDKILQSNSSG-DIVVTNDGATILKSIALDNAAAKVLVNI 83

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_YEAST (T-complex protein 1 subunit beta OS=Saccharomyces cerevisiae GN=CCT2 PE=1 SV=1)

HSP 1 Score: 152.14 bits (383), Expect = 3.926e-36
Identity = 79/118 (66.95%), Postives = 95/118 (80.51%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLV-AAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            +ISKVQDDEVGDGTTSV VL+ ELLREAEKL+  +KIHP TII G+R+A+  A DAL +  VDN  +   F+ DL+ IA TTLSSKILSQDK+HF +LA +A++RLKGSTNLE IQII
Sbjct:   79 NISKVQDDEVGDGTTSVTVLSAELLREAEKLIDQSKIHPQTIIEGYRLASAAALDALTKAAVDNSHDKTMFREDLIHIAKTTLSSKILSQDKDHFAELATNAILRLKGSTNLEHIQII 196

HSP 2 Score: 99.7525 bits (247), Expect = 2.313e-20
Identity = 50/78 (64.10%), Postives = 62/78 (79.49%), Query Frame = 2

Query:   77 RIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLVGI 310
            +IF D+  EE+ E AR+++FVGA+A+ DLVK+TLGPKGMDK+LQS        VTNDGATILKS+ +DNPAAKVLV I
Sbjct:    4 QIFGDQVTEERAENARLSAFVGAIAVGDLVKSTLGPKGMDKLLQSAS-SNTCMVTNDGATILKSIPLDNPAAKVLVNI 80

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_MACFA (T-complex protein 1 subunit beta OS=Macaca fascicularis GN=CCT2 PE=2 SV=3)

HSP 1 Score: 149.443 bits (376), Expect = 2.545e-35
Identity = 77/117 (65.81%), Postives = 94/117 (80.34%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            D+S+VQDDEVGDGTTSV VLA ELLREAE L+A KIHP TII+G+R A + AR+ALL   VD+  +  KF+ DLM IA TTLSSK+L+  K+HF +LAV+AV+RLKGS NLE+I II
Sbjct:   86 DMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHII 202

HSP 2 Score: 105.916 bits (263), Expect = 3.227e-22
Identity = 53/81 (65.43%), Postives = 65/81 (80.25%), Query Frame = 2

Query:   62 SMGVERIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLV 304
            S+    IFK  A+EE+ E AR+ SF+GA+AI DLVK+TLGPKGMDKIL S+GR   + VTNDGATILK++ +DNPAAKVLV
Sbjct:    5 SLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLV 85

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_HUMAN (T-complex protein 1 subunit beta OS=Homo sapiens GN=CCT2 PE=1 SV=4)

HSP 1 Score: 149.443 bits (376), Expect = 2.545e-35
Identity = 77/117 (65.81%), Postives = 94/117 (80.34%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            D+S+VQDDEVGDGTTSV VLA ELLREAE L+A KIHP TII+G+R A + AR+ALL   VD+  +  KF+ DLM IA TTLSSK+L+  K+HF +LAV+AV+RLKGS NLE+I II
Sbjct:   86 DMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHII 202

HSP 2 Score: 105.916 bits (263), Expect = 3.227e-22
Identity = 53/81 (65.43%), Postives = 65/81 (80.25%), Query Frame = 2

Query:   62 SMGVERIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLV 304
            S+    IFK  A+EE+ E AR+ SF+GA+AI DLVK+TLGPKGMDKIL S+GR   + VTNDGATILK++ +DNPAAKVLV
Sbjct:    5 SLAPVNIFKAGADEERAETARLTSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLV 85

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_BOVIN (T-complex protein 1 subunit beta OS=Bos taurus GN=CCT2 PE=2 SV=3)

HSP 1 Score: 148.288 bits (373), Expect = 5.670e-35
Identity = 76/117 (64.96%), Postives = 93/117 (79.49%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            D+S+VQDDEVGDGTTSV VLA ELLREAE L+A KIHP TII+G+R A + AR ALL   VD+  +  KF+ DLM IA TTLSSK+L+  K+HF +LAV+AV+RLKGS NLE+I +I
Sbjct:   86 DMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAARQALLNSAVDHGSDEVKFRQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHVI 202

HSP 2 Score: 106.301 bits (264), Expect = 2.471e-22
Identity = 53/81 (65.43%), Postives = 66/81 (81.48%), Query Frame = 2

Query:   62 SMGVERIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLV 304
            S+    IFK  A+EE+ E AR++SF+GA+AI DLVK+TLGPKGMDKIL S+GR   + VTNDGATILK++ +DNPAAKVLV
Sbjct:    5 SLAPVNIFKAGADEERAETARLSSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLV 85

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_MOUSE (T-complex protein 1 subunit beta OS=Mus musculus GN=Cct2 PE=1 SV=4)

HSP 1 Score: 147.517 bits (371), Expect = 9.671e-35
Identity = 76/117 (64.96%), Postives = 93/117 (79.49%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            D+S+VQDDEVGDGTTSV VLA ELLREAE L+A KIHP TIISG+R A + AR+ALL   VD+  +  +F  DLM IA TTLSSK+L+  K+HF +LAV+AV+RLKGS NLE+I +I
Sbjct:   86 DMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIISGWREATKAAREALLSSAVDHGSDEARFWQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHVI 202

HSP 2 Score: 105.916 bits (263), Expect = 3.227e-22
Identity = 53/81 (65.43%), Postives = 66/81 (81.48%), Query Frame = 2

Query:   62 SMGVERIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLV 304
            S+    IFK  A+EE+ E AR++SF+GA+AI DLVK+TLGPKGMDKIL S+GR   + VTNDGATILK++ +DNPAAKVLV
Sbjct:    5 SLAPVNIFKAGADEERAETARLSSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDAALMVTNDGATILKNIGVDNPAAKVLV 85

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_RAT (T-complex protein 1 subunit beta OS=Rattus norvegicus GN=Cct2 PE=1 SV=3)

HSP 1 Score: 147.132 bits (370), Expect = 1.263e-34
Identity = 76/117 (64.96%), Postives = 93/117 (79.49%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            D+S+VQDDEVGDGTTSV VLA ELLREAE L+A KIHP TII+G+R A + AR+ALL   VD+  +  KF  DLM IA TTLSSK+L+  K+HF +LAV+AV+RLKGS NLE+I +I
Sbjct:   86 DMSRVQDDEVGDGTTSVTVLAAELLREAESLIAKKIHPQTIIAGWREATKAAREALLSSAVDHGSDEVKFWQDLMNIAGTTLSSKLLTHHKDHFTKLAVEAVLRLKGSGNLEAIHVI 202

HSP 2 Score: 106.301 bits (264), Expect = 2.471e-22
Identity = 53/81 (65.43%), Postives = 66/81 (81.48%), Query Frame = 2

Query:   62 SMGVERIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLV 304
            S+    IFK  A+EE+ E AR++SF+GA+AI DLVK+TLGPKGMDKIL S+GR   + VTNDGATILK++ +DNPAAKVLV
Sbjct:    5 SLAPVNIFKAGADEERAETARLSSFIGAIAIGDLVKSTLGPKGMDKILLSSGRDASLMVTNDGATILKNIGVDNPAAKVLV 85

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_DICDI (T-complex protein 1 subunit beta OS=Dictyostelium discoideum GN=cct2 PE=1 SV=1)

HSP 1 Score: 144.821 bits (364), Expect = 6.269e-34
Identity = 73/117 (62.39%), Postives = 89/117 (76.07%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            DIS+ QDDEVGDGTTSV VLAGELLRE E+LV  K+HP TIISG+R+A E AR AL     D+  +  KF+ DL+ IA TTLSSKIL  +K+HF  + VDAV+RL G+TNL++I II
Sbjct:   85 DISRTQDDEVGDGTTSVCVLAGELLREGERLVQQKVHPQTIISGWRLALETARAALQSSTQDHSSDKVKFREDLLNIARTTLSSKILHTEKDHFANMVVDAVLRLNGNTNLDNIHII 201

HSP 2 Score: 104.375 bits (259), Expect = 9.389e-22
Identity = 53/82 (64.63%), Postives = 63/82 (76.83%), Query Frame = 2

Query:   80 IFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTGRGREVTVTNDGATILKSLHIDNPAAKVLVGILITVD 325
            I    A+EE+GE AR++SFVGA+AI DLVKTTLGPKGMDKIL S     E+ +TNDGATIL  ++IDNPAAK+LV I  T D
Sbjct:   10 ILNQNASEERGEIARLSSFVGAIAITDLVKTTLGPKGMDKILISASNPNELIITNDGATILTKIYIDNPAAKILVDISRTQD 91

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPB_CAEEL (T-complex protein 1 subunit beta OS=Caenorhabditis elegans GN=cct-2 PE=2 SV=2)

HSP 1 Score: 138.658 bits (348), Expect = 4.492e-32
Identity = 74/117 (63.25%), Postives = 91/117 (77.78%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVDNKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQII 897
            D+S  QD EVGDGTTSV VLA ELL+EAEKLV  +IHP TIISG+R A   A+++L +  +++ +N    + DL+KIA TTL SKILSQ KEHF QLAVDAV+RLKGS NL++IQII
Sbjct:   83 DMSMTQDHEVGDGTTSVTVLAAELLKEAEKLVNQRIHPQTIISGYRRALGIAQESLKKSSIESGDN---IRDDLLKIARTTLGSKILSQHKEHFAQLAVDAVLRLKGSGNLDAIQII 196

HSP 2 Score: 102.834 bits (255), Expect = 2.732e-21
Identity = 55/85 (64.71%), Postives = 66/85 (77.65%), Query Frame = 2

Query:   77 RIFKDEANEEKGERARMASFVGAMAIADLVKTTLGPKGMDKILQSTG--RGREVTVTNDGATILKSLHIDNPAAKVLVGILITVD 325
            +I KD A EE+GE AR++SFVGA+AI DLVK+TLGPKGMDKIL S        + VTNDGATILKS+ +DNPAAKVLV + +T D
Sbjct:    5 QILKDNAQEERGESARLSSFVGAIAIGDLVKSTLGPKGMDKILISGNPESAGGIKVTNDGATILKSIGVDNPAAKVLVDMSMTQD 89

[Close]

BLAST of CX673309 vs. ExPASy Swiss-Prot
Match: TCPH_PLAF7 (T-complex protein 1 subunit eta OS=Plasmodium falciparum (isolate 3D7) GN=PFC0350c PE=3 SV=1)

HSP 1 Score: 96.6709 bits (239), Expect = 1.958e-19
Identity = 57/117 (48.72%), Postives = 79/117 (67.52%), Query Frame = 1

Query:  547 DISKVQDDEVGDGTTSVVVLAGELLREAEKLVAAKIHPMTIISGFRMAAECARDALLQKVVD-NKENAEKFKSDLMKIAMTTLSSKILSQDKEHFGQLAVDAVMRLKGSTNLESIQI 894
            DI+K QDDEVGDGTTSVVV+AGELL EA+ L+   I P  II GFR A   A + L +  ++ + +N E+ +S L+K A T L+SK++S  KE FG+L V+AV +L  + +  +I I
Sbjct:   83 DIAKSQDDEVGDGTTSVVVVAGELLNEAKGLLNDGIEPNMIIDGFRNACNVAINKLNELSLNFSNKNEEEKRSILLKCAQTALNSKLVSNHKEFFGELVVNAVYKLGDNLDKSNIGI 199

The following BLAST results are available for this feature:

BLAST of CX673309 vs. ExPASy Swiss-Prot

BLAST of CX673309 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 104


Match Name	E-value	Identity	Description
TCPB_SCHPO	3.006e-36	68.38	Probable T-complex protein 1 subunit beta OS=Schiz...	[more]
TCPB_YEAST	3.926e-36	66.95	T-complex protein 1 subunit beta OS=Saccharomyces ...	[more]
TCPB_MACFA	2.545e-35	65.81	T-complex protein 1 subunit beta OS=Macaca fascicu...	[more]
TCPB_HUMAN	2.545e-35	65.81	T-complex protein 1 subunit beta OS=Homo sapiens G...	[more]
TCPB_BOVIN	5.670e-35	64.96	T-complex protein 1 subunit beta OS=Bos taurus GN=...	[more]
TCPB_MOUSE	9.671e-35	64.96	T-complex protein 1 subunit beta OS=Mus musculus G...	[more]
TCPB_RAT	1.263e-34	64.96	T-complex protein 1 subunit beta OS=Rattus norvegi...	[more]
TCPB_DICDI	6.269e-34	62.39	T-complex protein 1 subunit beta OS=Dictyostelium ...	[more]
TCPB_CAEEL	4.492e-32	63.25	T-complex protein 1 subunit beta OS=Caenorhabditis...	[more]
TCPH_PLAF7	1.958e-19	48.72	T-complex protein 1 subunit eta OS=Plasmodium falc...	[more]

Pages

Properties


Property Name	Value
Genbank description	UCRCS10_21H02_b Madame Vinous Sweet Orange Multiple Pathogen-Infected cDNA Library UCRCS10 Citrus sinensis cDNA clone UCRCS10-21H02-O3-5.b, mRNA sequence.

Sequences

The following sequences are available for this feature:

EST sequence

EST sequence

>CX673309 ID=CX673309; Name=CX673309; organism=Citrus sinensis; type=EST; length=899bp
AAAACCTAGCAAGGGTTTCATTTTAGCATCCAGGTTTTAGTTGCCTTCAT
TTACATTTTCTTCGATGGGGGTAGAGAGAATTTTTAAAGATGAAGCTAAC
GAAGAGAAAGGAGAGCGTGCCAGAATGGCATCATTTGTTGGTGCAATGGC
AATTGCTGACTTGGTTAAGACAACCTTAGGGCCAAAGGGAATGGATAAAA
TTCTACAGTCAACCGGCAGAGGACGTGAAGTCACTGTTACGAATGACGGG
GCCACTATACTTAAGTCCCTACACATTGATAATCCAGCTGCTAAAGTCCT
TGTTGGTATCCTTATTACTGTTGATTTGCTGCAATGGTGGGTTAATGTAT
TACTGTTGATTTGTTGTAATGGTGCGCTGATGTTTATTTTGTTTTCCTGG
AAAAAAAAAAAATACTGTGTAAGCATTTTTTCAGGAAAAACTCTTTAAGT
GGTGCAATTAGCCACGCCAGTAGAATAGTTACACATAACCCATAGAAAAA
AGATTATTCCTTCTTCCTTTGCTTTATGATCCTTAATCAAGGGTCAGACA
TCTCTAAGGTTCAGGATGATGAAGTTGGTGATGGGACAACTTCAGTTGTT
GTTTTGGCTGGGGAGCTTCTGAGGGAGGCAGAGAAGCTGGTGGCTGCTAA
GATTCACCCAATGACCATAATATCAGGTTTCCGGATGGCAGCTGAATGCG
CTCGCGATGCTTTGTTGCAAAAGGTTGTGGATAACAAAGAGAATGCAGAA
AAATTCAAGTCAGACTTGATGAAGATTGCGATGACTACTTTGAGTTCAAA
AATTCTCTCACAGGATAAAGAACATTTTGGTCAACTGGCTGTGGATGCTG
TTATGAGGCTTAAGGGCAGCACAAATTTAGAGTCCATCCAAATTATCAA