EY664338

Overview
NameEY664338
Unique NameEY664338
TypeEST
OrganismCitrus sinensis (Sweet orange)
Sequence length899
Analyses
This EST is derived from or has results from the following analyses
Analysis NameDate Performed
BLAST: Citrus ESTs to Prunus persica proteins V12010-05-10
BLAST: Citrus ESTs to Populus V2 proteins2010-05-10
BLAST: Citrus ESTs to TAIR92010-05-10
BLAST: Citrus ESTs to SwissProt2010-05-10
Alignments
Feature NameTypeLocationAnalysis
EY664338_ssr18 microsatellite EY664338_ssr18:18..27. BLAST: Citrus ESTs to Prunus persica proteins V1
Homology
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSBL7_HUMAN (Oxysterol-binding protein-related protein 7 OS=Homo sapiens GN=OSBPL7 PE=2 SV=1)

HSP 1 Score: 78.9518 bits (193), Expect = 1.307e-23
Identity = 41/87 (47.13%), Postives = 60/87 (68.97%), Query Frame = 3 
Query:  303 WKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFA--YRRAWKPFNPVLGETFE 557
            W +++  IG D+ S V++PV ++EP + LQR+ E +EYS LLD A    DP  R+VY +++A+S Y +  +R   KPFNPVLGET+E
Sbjct:  480 WNILRNNIGKDL-SKVSMPVQLNEPLNTLQRLCEELEYSSLLDQASRIADPCERMVYIAAFAVSAYSSTYHRAGCKPFNPVLGETYE 565

HSP 2 Score: 52.373 bits (124), Expect = 1.307e-23
Identity = 26/58 (44.83%), Postives = 36/58 (62.07%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDVYPVERHV*HLKRWG 744
            G  FI+EQ SHHPP+SA HA +E+F +    K + KF G S+++ PV      L R+G
Sbjct:  572 GFRFISEQVSHHPPISACHAESENFAFWQDMKWKNKFWGKSLEIVPVGTVNVSLPRFG 629
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSBL6_HUMAN (Oxysterol-binding protein-related protein 6 OS=Homo sapiens GN=OSBPL6 PE=1 SV=1)

HSP 1 Score: 83.1889 bits (204), Expect = 2.210e-23
Identity = 43/89 (48.31%), Postives = 62/89 (69.66%), Query Frame = 3 
Query:  297 NYWKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFA--YRRAWKPFNPVLGETFE 557
            N W +++  IG D+ S V++PV ++EP + LQ + E MEYS LLD A E +DPY R+V  +++A+S Y +  +R   KPFNPVLGET+E
Sbjct:  570 NLWNILRNNIGKDL-SKVSMPVELNEPLNTLQHLCEEMEYSELLDKASETDDPYERMVLVAAFAVSGYCSTYFRAGSKPFNPVLGETYE 657

HSP 2 Score: 47.3654 bits (111), Expect = 2.210e-23
Identity = 19/47 (40.43%), Postives = 31/47 (65.96%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDVYPV 711
            G  F +EQ SHHPP+SA H  +++F++    + + KF G S+++ PV
Sbjct:  664 GFRFFSEQVSHHPPISACHCESKNFVFWQDIRWKNKFWGKSMEILPV 710
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSBL3_HUMAN (Oxysterol-binding protein-related protein 3 OS=Homo sapiens GN=OSBPL3 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 1.816e-22
Identity = 41/87 (47.13%), Postives = 59/87 (67.82%), Query Frame = 3 
Query:  303 WKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFA--YRRAWKPFNPVLGETFE 557
            W +++  IG D+ S V +PV ++EP + LQR+ E +EYS LLD A +   P  R+VY +++A+S Y +  YR   KPFNPVLGET+E
Sbjct:  529 WNILRNNIGKDL-SKVAMPVELNEPLNTLQRLCEELEYSELLDKAAQIPSPLERMVYVAAFAISAYASSYYRAGSKPFNPVLGETYE 614

HSP 2 Score: 48.1358 bits (113), Expect = 1.816e-22
Identity = 19/47 (40.43%), Postives = 31/47 (65.96%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDVYPV 711
            G  F +EQ SHHPP+SA HA + +F++    + + KF G S+++ P+
Sbjct:  621 GFQFFSEQVSHHPPISACHAESRNFVFWQDVRWKNKFWGKSMEIVPI 667
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: YB35_SCHPO (Oxysterol-binding protein homolog C2F12.05c OS=Schizosaccharomyces pombe GN=SPBC2F12.05c PE=1 SV=2)

HSP 1 Score: 82.0333 bits (201), Expect = 2.348e-22
Identity = 42/87 (48.28%), Postives = 61/87 (70.11%), Query Frame = 3 
Query:  303 WKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFA-YRRAWKPFNPVLGETFEW 560
            W +++  IG D+  + TLPV  +EP S+LQR+AE MEY+ LLD A   +DPY R++Y +++A S Y +   R  KPFNP+LGET+E+
Sbjct:  939 WGILKGMIGKDMTKM-TLPVSFNEPTSLLQRVAEDMEYTELLDQASHNKDPYQRILYVAAFAASEYASTLNRVAKPFNPLLGETYEF 1024

HSP 2 Score: 45.0542 bits (105), Expect = 2.348e-22
Identity = 19/47 (40.43%), Postives = 29/47 (61.70%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDVYPV 711
            G  FI EQ SHHPP+ A ++ + ++ Y     V++KF G S D+ P+
Sbjct: 1030 GFRFIVEQVSHHPPVGAAYSESANWKYYGESSVKSKFYGKSFDISPL 1076
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSBL3_MOUSE (Oxysterol-binding protein-related protein 3 OS=Mus musculus GN=Osbpl3 PE=1 SV=1)

HSP 1 Score: 77.7962 bits (190), Expect = 4.001e-22
Identity = 40/87 (45.98%), Postives = 58/87 (66.67%), Query Frame = 3 
Query:  303 WKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFA--YRRAWKPFNPVLGETFE 557
            W +++  IG D+ S V +PV ++EP + LQR+ E +EYS LLD A     P  R+VY +++A+S Y +  +R   KPFNPVLGET+E
Sbjct:  497 WSILRNNIGKDL-SKVAMPVELNEPLNTLQRLCEELEYSELLDKASRIPSPLERMVYVAAFAISAYASSYFRAGSKPFNPVLGETYE 582

HSP 2 Score: 48.521 bits (114), Expect = 4.001e-22
Identity = 20/47 (42.55%), Postives = 31/47 (65.96%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDVYPV 711
            G  F AEQ SHHPP+SA HA + +F++    + + KF G S+++ P+
Sbjct:  589 GFQFFAEQVSHHPPISACHAESGNFVFWQDVRWKNKFWGKSMEIVPI 635
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSB8_DICDI (Oxysterol-binding protein 8 OS=Dictyostelium discoideum GN=osbH PE=1 SV=1)

HSP 1 Score: 85.1149 bits (209), Expect = 8.934e-22
Identity = 47/124 (37.90%), Postives = 74/124 (59.68%), Query Frame = 3 
Query:  189 NGLLGY-EGLEVVNPEGSTEDAEDEARRGRWKQEERDNYWKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFAYRRAWKPFNPVLGETFE 557
            +G L Y +GL   N     E++ D   +G  + E+R    K +  Y+G D+ SL++LPV+I EP S LQ M+E ++Y+ LL  A + +  ++ L Y +++  ++Y    R  KPFNP+LGETFE
Sbjct:    3 SGALNYMKGLVGTNENLQVEESGDN--KGDIEPEQRKGLLKQLSSYVGKDITSLISLPVWIFEPVSFLQVMSEPLQYNALLSKASKQDSEFLCLAYLAAFNCALYSTAVRTRKPFNPILGETFE 124

HSP 2 Score: 40.0466 bits (92), Expect = 8.934e-22
Identity = 17/45 (37.78%), Postives = 26/45 (57.78%), Query Frame = 1 
Query:  568 GGXTFIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDV 702
            G   F+AEQ SHHPP+      +E +   +   +++KF GNS +V
Sbjct:  130 GEFRFLAEQVSHHPPIGVSETISEDYTLQLETLLKSKFYGNSSEV 174
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSB11_DICDI (Oxysterol-binding protein 11 OS=Dictyostelium discoideum GN=osbK PE=3 SV=1)

HSP 1 Score: 80.8777 bits (198), Expect = 5.959e-20
Identity = 36/92 (39.13%), Postives = 60/92 (65.22%), Query Frame = 3 
Query:  285 EERDNYWKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFAYRRAWKPFNPVLGETFEW 560
            E++ + WK +   +G D  SLV+LPV+  EP +VL+   E + +  L++ A  C D   RL+Y +++ ++++ +Y R  KPFNP+LGETFE+
Sbjct:   88 EDKKSLWKKVGGLVGKDPMSLVSLPVYFFEPLTVLECQLEPLRFVELIEKASTCSDSIDRLMYLTAFNIAVFSSYTRTAKPFNPLLGETFEY 179

HSP 2 Score: 38.1206 bits (87), Expect = 5.959e-20
Identity = 17/39 (43.59%), Postives = 22/39 (56.41%), Query Frame = 1 
Query:  589 EQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDVY 705
            EQ SHHPP+      +E F       + TKF GNS+DV+
Sbjct:  191 EQVSHHPPIGIAQTTSEIFDLQQESWITTKFWGNSLDVF 229
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSBL1_MOUSE (Oxysterol-binding protein-related protein 1 OS=Mus musculus GN=Osbpl1a PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 5.127e-18
Identity = 35/86 (40.70%), Postives = 57/86 (66.28%), Query Frame = 3 
Query:  303 WKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALS-IYFAYRRAWKPFNPVLGETFE 557
            W +++K IG ++ S +T+PV  +EP S LQR+ E ME+++L+  A    DP  R+   +++A+S +   + R  KPFNP+LGET+E
Sbjct:  550 WSILRKCIGMEL-SKITMPVIFNEPLSFLQRLTEYMEHTYLIHKASSLSDPVERMQCVAAFAVSAVASQWERTGKPFNPLLGETYE 634

HSP 2 Score: 41.9726 bits (97), Expect = 5.127e-18
Identity = 21/48 (43.75%), Postives = 28/48 (58.33%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHAR--NEHFIYDVTXKVRTKFLGNSIDVYP 708
            G   I+EQ SHHPP+SA HA   N  FI+  +   + KF G S++  P
Sbjct:  641 GFRLISEQVSHHPPISAFHAEGLNNDFIFHGSIYPKLKFWGKSVEAEP 688
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSBL1_HUMAN (Oxysterol-binding protein-related protein 1 OS=Homo sapiens GN=OSBPL1A PE=1 SV=2)

HSP 1 Score: 70.4774 bits (171), Expect = 5.127e-18
Identity = 35/86 (40.70%), Postives = 57/86 (66.28%), Query Frame = 3 
Query:  303 WKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALS-IYFAYRRAWKPFNPVLGETFE 557
            W +++K IG ++ S +T+PV  +EP S LQR+ E ME+++L+  A    DP  R+   +++A+S +   + R  KPFNP+LGET+E
Sbjct:  550 WSILRKCIGMEL-SKITMPVIFNEPLSFLQRLTEYMEHTYLIHKASSLSDPVERMQCVAAFAVSAVASQWERTGKPFNPLLGETYE 634

HSP 2 Score: 41.9726 bits (97), Expect = 5.127e-18
Identity = 21/48 (43.75%), Postives = 28/48 (58.33%), Query Frame = 1 
Query:  571 GXTFIAEQXSHHPPMSAGHAR--NEHFIYDVTXKVRTKFLGNSIDVYP 708
            G   I+EQ SHHPP+SA HA   N  FI+  +   + KF G S++  P
Sbjct:  641 GFRLISEQVSHHPPISAFHAEGLNNDFIFHGSIYPKLKFWGKSVEAEP 688
[Close]
BLAST of EY664338 vs. ExPASy Swiss-Prot
Match: OSH2_YEAST (Oxysterol-binding protein homolog 2 OS=Saccharomyces cerevisiae GN=OSH2 PE=1 SV=1)

HSP 1 Score: 79.337 bits (194), Expect = 6.627e-18
Identity = 49/120 (40.83%), Postives = 73/120 (60.83%), Query Frame = 3 
Query:  216 EVVNPEGSTEDAEDEARRGRWKQEERD----NYWKMMQKYIGADVQSLVTLPVFISEPNSVLQRMAELMEYSHLLDLADECEDPYMRLVYASSWALSIYFA-YRRAWKPFNPVLGETFEW 560
            E +  EGS    ED  R+ R   ++ D    + W +++  +G D+  + TLPV  +EP S+LQR+AE +EYS LLD A   ED  +R +Y +++  S Y +  +R  KPFNP+LGETFE+
Sbjct:  867 EYLLKEGSYLGYEDGIRK-RLSMDKDDRPKISLWAVLKSMVGKDMTRM-TLPVTFNEPTSLLQRVAEDLEYSELLDQAATFEDSTLRTLYVAAFTASSYASTTKRVAKPFNPLLGETFEY 984

HSP 2 Score: 32.7278 bits (73), Expect = 6.627e-18
Identity = 17/41 (41.46%), Postives = 22/41 (53.66%), Query Frame = 1 
Query:  580 FIAEQXSHHPPMSAGHARNEHFIYDVTXKVRTKFLGNSIDV 702
            F  EQ SHHPP+SA    +  + +     V TKF G S +V
Sbjct:  993 FFTEQVSHHPPISATWTESPRWDFWGESFVDTKFNGRSFNV 1033
The following BLAST results are available for this feature:
BLAST of EY664338 vs. ExPASy Swiss-Prot
Analysis Date: 2010-05-10 (BLAST: Citrus ESTs to SwissProt)
Total hits: 28
Match NameE-valueIdentityDescription
OSBL7_HUMAN1.307e-2347.13Oxysterol-binding protein-related protein 7 OS=Hom... [more]
OSBL6_HUMAN2.210e-2348.31Oxysterol-binding protein-related protein 6 OS=Hom... [more]
OSBL3_HUMAN1.816e-2247.13Oxysterol-binding protein-related protein 3 OS=Hom... [more]
YB35_SCHPO2.348e-2248.28Oxysterol-binding protein homolog C2F12.05c OS=Sch... [more]
OSBL3_MOUSE4.001e-2245.98Oxysterol-binding protein-related protein 3 OS=Mus... [more]
OSB8_DICDI8.934e-2237.90Oxysterol-binding protein 8 OS=Dictyostelium disco... [more]
OSB11_DICDI5.959e-2039.13Oxysterol-binding protein 11 OS=Dictyostelium disc... [more]
OSBL1_MOUSE5.127e-1840.70Oxysterol-binding protein-related protein 1 OS=Mus... [more]
OSBL1_HUMAN5.127e-1840.70Oxysterol-binding protein-related protein 1 OS=Hom... [more]
OSH2_YEAST6.627e-1840.83Oxysterol-binding protein homolog 2 OS=Saccharomyc... [more]

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Properties
Property NameValue
Genbank descriptionCS00-C1-101-065-E06-CT.F Sweet orange leaf, infected with Xylella fastidiosa (stage 1 of 2) Citrus sinensis cDNA, mRNA sequence.
Sequences
The following sequences are available for this feature:

EST sequence

>EY664338 ID=EY664338; Name=EY664338; organism=Citrus sinensis; type=EST; length=899bp
CCACGCGTCCGAAAACGTCTCTCTCTCTATCCTCTCTAAGCTACGTATTC
ATTCGATAGCTGCGAGAATTTTCTCCTGAGATTGTTAATTGAGACATGGC
TTCCGATGGATCTAAGCAAGGCGGCGGTTTCTTCGCCTCCATCGCCGCCG
GTTGGTCGACTTTCGGCTCCGCCATGAGTAAATCGGTCAACGGTTTATTG
GGCTATGAAGGGCTGGAAGTTGTTAATCCTGAAGGGAGCACGGAAGATGC
TGAAGATGAAGCAAGAAGAGGAAGATGGAAACAGGAGGAAAGGGACAATT
ACTGGAAGATGATGCAAAAGTATATAGGTGCTGATGTTCAATCGTTGGTG
ACTCTTCCTGTATTTATTTCAGAGCCAAATTCAGTGCTGCAGAGAATGGC
AGAGTTGATGGAGTACTCCCACCTTCTGGATCTGGCAGATGAGTGCGAGG
ATCCATACATGAGATTGGTGTATGCTTCATCATGGGCTTTATCAATCTAC
TTTGCCTATCGACGGGCCTGGAAGCCATTTAATCCAGTTCTTGGTGAAAC
TTTTGAATGGTAATCATGGNGGTGNTACATTTATAGCTGAACAAGNTAGT
CATCACCCCCCAATGAGTGCTGGTCATGCCCGAAATGAGCACTTCATTTA
TGACGTGACTTNCAAAGTGAGAACAAAATTTTTAGGAAACTCGATTGATG
TTTATCCAGTTGAAAGGCACGTGTGACACTTAAAGAGATGGGGTGGGCCT
CGATTTGGTGCCCCCTCCACAAAGGTTCCAATTAATTTTTGGACAACCTG
GATGGTCACCGGGGGAATTGGCATGACTACTTGACCCCGGCGAAAAATGT
GGGTTTTTTTTAACCTCCCGATGGTTGGACCGGCCCTTAAAAGGGAGGA
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